Seed Germination and Growth Effects of Endophyte Infection on Melica transsilvanica under Cd Stress

doi:10.6048/j.issn.1001-4330.2018.10.019

Abstract

Abstract: 【Objective】 To study the effects of Cd on (E+) (E-) seed germination, plant growth physiology and Cd content in plants in the hope of providing theoretical basis for evaluating symbiotic microorganisms to enhance plant heavy metal tolerance and controlling heavy metal Cd pollution.【Method】 The seeds infected with Melica transsilvanica (endophyte infected, E) and non-infected Melica transsilvanica (endophyte free, E-) were used as the experimental materials and different concentrations of (cadmium) Cd were applied to carry out stress germination experiment on the seeds of Melica transsilvanica, the purpose of which was to analyze the effects of endophytic fungal infection on the germination and growth of host seeds under heavy metal Cd stress.【Result】 (1) When the concentration of Cd was lower than 30 mg/L, the germination indexes of seeds were higher than the contrast. When the concentration of Cd was higher than 30 mg/L, the germination indexes of seeds showed a downward trend. When the concentration of Cd was higher than 200 mg/L, the seed germination was strongly inhibited. (2) With the increase of stress concentration, the dry matter content of both shoots and roots increased, and the Cd transfer coefficient increased gradually. (3) When the concentration of Cd was lower than 150 mg/L, the germination rate, germination potential and vitality index of E+ were significantly higher than those of E- at the same concentration (P<0.05), and the content of heavy metals in roots was significantly higher than that of the buds at the same concentration (P<0.05). The content of Cd in E- plants was significantly higher than that in E+ plants (P<0.05).【Conclusion】 Endophyte infection effectively alleviated the toxicity of Cd on seed germination and growth, which further confirmed that symbiotic microorganisms could enhance the tolerance of Cd on the host M. transsilvanica.

Key words: Cd stress; endophyte; Melica transsilvanica; seed germination

摘要： 【目的】 研究Cd对(E+)(E-)种子萌发、植株生长生理及植株内Cd含量的变化规律,为评价共生微生物增强植物重金属耐性和重金属Cd污染防治提供理论依据。【方法】 以德兰臭草感染内生真菌(endophyte infected,E+)与不感染内生真菌(endophyte free,E-)的种子为材料,用不同浓度的(镉)Cd对德兰臭草种子进行胁迫萌发试验,分析重金属Cd胁迫下内生真菌感染对宿主种子萌发及生长的影响。【结果】 Cd胁迫浓度低于30 mg/L时,种子萌发指标均高于对照,胁迫浓度高于30 mg/L时,种子的各萌发指标呈下降趋势,胁迫浓度达到200 mg/L以上时,强烈抑制种子萌发。随着胁迫浓度的升高,无论是幼芽还是幼根,干物质的量都呈现增加的趋势,且Cd转移系数逐渐增大。胁迫浓度低于150 mg/L时,E+的发芽率、发芽势、活力指数均显著高于同一浓度下的E-(P<0.05),且德兰臭草幼根中的重金属含量显著高于同一浓度下的幼芽(P<0.05),E-植株中的Cd含量显著高于E+植株(P<0.05)。【结论】 内生真菌的侵染有效缓解了Cd对德兰臭草种子萌发和生长的毒害作用,证实了共生微生物能增强宿主德兰臭草对重金属Cd的耐受性。

关键词: 重金属Cd胁迫, 内生真菌, 德兰臭草, 种子萌发

CLC Number:

S182

LI Ke, SHI Chong , LI Hao-yu, WANG Wen-quan, LI Yang. Seed Germination and Growth Effects of Endophyte Infection on Melica transsilvanica under Cd Stress[J]. Xinjiang Agricultural Sciences, 2018, 55(10): 1916-1926.

李柯,施宠,李昊宇,王文全,李阳. 重金属Cd胁迫下内生真菌感染对德兰臭草种子萌发及生长的影响[J]. 新疆农业科学, 2018, 55(10): 1916-1926.

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