Identification of Pathogenic Fungi That Infect Leaves and Fruits of Jujube

doi:10.6048/j.issn.1001-4330.2018.09.014

Xinjiang Agricultural Sciences ›› 2018, Vol. 55 ›› Issue (9): 1682-1688.DOI: 10.6048/j.issn.1001-4330.2018.09.014

Previous Articles Next Articles

Identification of Pathogenic Fungi That Infect Leaves and Fruits of Jujube

Sajidamu Aizezi¹, Mahemutijiang MIjiti¹, ZHAO Zhi-qiang¹, XU Tai-bai¹, GUO Qing-yuan¹

College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China

Received:2018-07-18 Online:2018-09-20 Published:2018-12-07
Correspondence author: GUO Qing-yuan(1962-),Sichuan people, Professor of college of Agronomy, Xinjiang Agricultural University, working on plant pathology,(E-mail)guoqingyuan3009@sina.com
Supported by:
Science and Technology Planning project of Xinjiang Uygur Autonomous Region " Integration and demonstration of Key Technologies Efficient and Safe Production of Featured Fruit Trees in Xinjiang" (201130102);Scientific Research Innovation Project for Postgraduate Students of Xinjiang Agricultural University(XJAUGRI16-019)

一种侵染红枣叶片和果实的病原真菌鉴定

萨吉达木·艾则孜, 麦合木提江·米吉提, 赵志强, 许泰百, 郭庆元

新疆农业大学,乌鲁木齐 830052

通讯作者: 郭庆元(1962-),四川人,教授,博士,研究方向为植物病理,(E-mail)guoqingyuan3009@sina.com
作者简介:萨吉达木·艾则孜(1992-),新疆人,硕士研究生,研究方向为植物病理,(E-mail)1539475398@qq.com
基金资助:
新疆维吾尔自治区科技计划项目“新疆特色果树高效安全生产关键技术研究集成与示范”(201130102);新疆农业大学研究生科研创新项目(XJAUGRI16-019)

Abstract

Abstract: 【Objective】 In order to clarify the recent occurrence of a species of pathogenic fungi that is closely related to Jujube mosaic disease in Xinjiang.【Method】The pathogen was identified by collecting disease samples, isolating pathogenic bacteria, recovering syndromes, morphological observation and rDNA-ITS sequence alignment.【Result】The spore strains were isolated by some significant mosaic and brown leaf spot samples and abnormal fruit samples. After inoculation of jujube leaves and young fruits with acupuncture, brown necrotic spots similar to the field symptoms were found, and the pathogenicity of them to the leaves and fruits of jujube was determined. The morphological characteristics of the pathogen were basically the same as that of Peyronellaea glomerata, and the homology between the rDNA-ITS sequence of three isolates and that of Peyronellaea glomerata was 99%.【Conclusion】On the basis of morphological and ITS sequence information, the pathogen of the new disease of jujube was identified as Peyronellaea glomerata in Xinjiang.

Key words: Jujube; brown necrosis spot Peyronellaea glomerata; morphology; molecular identification

摘要： 【目的】研究新疆地区近年发生的一种与枣花叶病有密切关系的病原真菌。【方法】通过病样采集、病原菌分离、回接证病,以及形态观察和rDNA-ITS序列比对等方法,鉴定病原菌。【结果】从有明显花叶症状和褐色叶斑的病叶以及有畸果症状和褐色斑点的病果中分离获得多个真菌单孢菌株,用针刺法将其接种到枣叶和幼果后出现与田间症状基本相似的褐色坏死斑,确定其对枣叶、果的致病性;该菌病原菌形态特征与头状茎点霉基本一致,3个分离菌株的rDNA-ITS序列与头状茎点霉的同源性达到99%。【结论】根据病原菌的形态特征和分子鉴定结果,将该病原菌鉴定为头状茎点霉(Peyronellaea. glomerata)。

关键词: 红枣, 褐色坏死斑, 头状茎点霉, 形态特征, 分子鉴定

CLC Number:

S665.1
S188

Sajidamu Aizezi, Mahemutijiang MIjiti, ZHAO Zhi-qiang, XU Tai-bai, GUO Qing-yuan. Identification of Pathogenic Fungi That Infect Leaves and Fruits of Jujube[J]. Xinjiang Agricultural Sciences, 2018, 55(9): 1682-1688.

萨吉达木·艾则孜, 麦合木提江·米吉提, 赵志强, 许泰百, 郭庆元. 一种侵染红枣叶片和果实的病原真菌鉴定[J]. 新疆农业科学, 2018, 55(9): 1682-1688.

References

[1] 刘孟军.中国红枣产业的现状与发展建议[J].果农之友,2008,9(3):3-4, 26.
   LU Meng-jun. (2008). The present situation and development proposal of Chinese red dates industry [J]. Friends of the Fruit, 9(3):3-4, 26. (in Chinese)
[2] 蒋晓晓.阿克苏枣树主要害虫的发生规律与生态位研究[D].乌鲁木齐:新疆农业大学硕士论文,2014.
   JIANG Xiao-xiao. (2014). Study on Occurrence Regularity and Ecological Niche of Main Pests in Akesu Jujube [D]. Master Dissertation. Xinjiang Agricultural University, Urumqi. (in Chinese)
[3] Julaf. Morton. (1987). Frutis of warm climates [M]. Indian Jujube, Florida: Miami press: 272-275.
[4] 赵燕. 新疆红枣病害种类调查及枣缩果病病原的相关研究[D].乌鲁木齐:新疆农业大学硕士论文,2014.
   ZHAO Yan. (2014). Investigation of Chinese Jujube Disease in Xinjiang and Research on the Pathogen of Jujube Fruit Shrink Disease [D]. Master Dissertation. Xinjiang Agricultural University, Urumqi. (in Chinese)
[5] Bai, J. Y., Guo, Q. Y., Wang, X. M., & Duan, C. X. (2015). First report of jujube leaf spot caused by alternaria alternata in china. Plant Disease, 99(11): 1,643.
[6] Bai, J., Wang, X., Shi, Y., & Duan, C. (2016). Occurrence and identification of Nothophoma quercina causing brown spot of jujube in China. Canadian Journal of Plant Pathology, 38(4): 527-532.
[7] Boerema, G. H., Gruyter, J. D., Noordeloos, M. E., Hamers, M. E. C., Boerema, G. H., & Gruyter, J. D., et al. (2004). Phoma identification manual. differentiation of specific and infra-specific taxa in culture. European Journal of Plant Pathology, 111(3): 295-296.
[8] Ligoxigakis, E. K., Markakis, E. A., Papaioannou, I. A., & Typas, M. A. (2013). First report of petiole (rachis) blight ofWashingtonian filifera caused by phoma glomerata in Greece. Plant Disease, 97(11): 1,509.
[9] Aghapour, B., Fotouhifar, K. B., Ahmadpour, A., & Ghazanfari, K. (2009). First report of leaf spot disease on ficus elastica caused by Phoma glomerat in Iran. Australasian Plant Disease Notes, 4(1): 82-83.
   Zhao, D., & Kang, Y. B. (2013). First report of branch blight of tree peony caused by Phoma glomerata in China. Plant Disease, 97(8): 1,114.
[10] Wang, X., Wang, J., Gao, J., & Yang, L. (2012). First report of leaf spot disease on schisandra chinensis caused by Phoma glomerata in China. Plant Disease, 96(2): 289.
[11] Hatami N, Zamanideh H, Aminaii M M. (2008). Collection and identification of plant pathogenic fungi in cucumber greenhouses. Proceeding of the 18th Iranian Plant Protection Congress[C]. University of Buali Sina: 195.
[12] 段维军,顾建锋,张慧丽,等.进境意大利苹果苗上葡萄茎枯病菌的截获鉴定[J].植物病理学报,2014,44(5):542-545.
   DUAN Wei-jun, GU Jian-feng, ZHANG Hui-li, et al. (2014). Interception and identification ofPhoma glomerata on apple (Mallus sp) seed-lingsim ported from Italy [J]. Acta Phytopathologica Sinica, 44(5):542-545. (in Chinese)
[13] Moral, J., Lichtemberg, P. S. F., Papagelis, A., Sherman, J., & Michailides, T. J. (2018). Didymella glomerata, causing leaf blight onpistachio. European Journal of Plant Pathology: 1-5.
[14] Milatovic, I., Masten, V., & Kadic, B. (1960). An experiment on the control of flower blight, peyronellaea glomerata, on vines in Istria. Zastita Bilja: 57-60.
[15] Yang, C. D, Yao, Y. L, Zhang, Z. F, Xue, L. (2016). First Report of Leaf blight ofPoa pratensis Caused by Peyronellaea glomerata in China.Plant Disease, 100(4): 862-863.
[16] Tran, H. S., You, M. P., Lanoiselet, V., Khan, T. N., & Barbetti, M. J. (2014). First report ofPhoma glomerata associated with the ascochyta blight complex on field pea (Pisum sativum) in Australia. Plant Disease,98(3): 427.
[17] Brooks, F. T., & Searle, G. O. (1921). An investigation of some tomato diseases. Transactions of the British Mycological Society, 7(3): 173-197.
[18] Huang, S., Wang, L., Wang, T., Jiao, Z., Pang, F. H., & Tao, A., et al. (2018). First report of didymella leaf blight on cornus officinalis caused by D. glomerata in China. Plant Disease, 102(5):.

Identification of Pathogenic Fungi That Infect Leaves and Fruits of Jujube

一种侵染红枣叶片和果实的病原真菌鉴定

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

[1]	JIN Juan, LI lili, YANG Lei, FAN Dingyu, HAO Qing. Analysis on the Development status of Xinjiang Jujube Industry [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 106-110.
[2]	SHEN Yuyang, HONG Gaojie, FAN Guiqiang, CHEN Li, LEI Junjie, LI Guangkuo, GAO Haifeng. The control effect of reduced pesticides application and adjuvant addition on wheat aphids in jujube-wheat intercropping pattern [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2257-2268.
[3]	ZHAO Suya, YANG Yanai, ZHOU Ling, LYU Xifeng, YAO Jiang, LU Yaling. Preparation of jujube based carbon slow release fertilizer with starch as adhesive and its release property analysis [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2315-2322.
[4]	LI TANG, LI Chunyan, MENG Ziwei, LI Yapeng, ZHANG Wangbin. Population structure analysis of pathogen of pear valsa canker in Aral area of Xinjiang [J]. Xinjiang Agricultural Sciences, 2024, 61(3): 681-689.
[5]	MENG Yina, CHEN Jing, WANG Ziming, ZHANG Ting, HAO Qing, WU Bin, ZHANG Ping, GUO Limin. Screening of treatment methods for gray jujube storage period and establishment of prediction model [J]. Xinjiang Agricultural Sciences, 2024, 61(12): 2997-3005.
[6]	Gulidan Sailikebole, WANG Chengxin, XU Hao, HU Bolin, YANG Haoke, WEI Qi, CHEN Shengao. Research on the biology of Triplophysa strauchiiin Turks River [J]. Xinjiang Agricultural Sciences, 2024, 61(10): 2576-2582.
[7]	LI Ziqin, CHEN Ya, LI Wenqi, JIA Wenting, GUO Huijing, SONG Fangyuan, ZHAO Zhiyong, LIU Chengjiang. Effects of different precooling methods combined with H₂O₂treatment on the quality of lvtangxin winter jujube during storage [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2208-2215.
[8]	LI Kailiang, ZHANG Zhenyu, HU Hongying. Structure and diversity analysis of insect community in jujube orchard of Hami Area in Xinjiang [J]. Xinjiang Agricultural Sciences, 2023, 60(8): 2028-2037.
[9]	ZHANG Yanfei, Subina Xaokelaiti, YANG Lei, HAO Qing, JIN Juan, FAN Dingyu. SSR fingerprinting and genetic diversity analysis of 26 fresh jujube cultivars [J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1671-1678.
[10]	YANG Xiaojuan, JIN Juan, FAN Dingyu, HAO Qing, YANG Lei, GENG Wenjuan. Effects of extreme high temperature environment on photosynthetic characteristics of Junzao and Fucuimi [J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1679-1688.
[11]	LUO Siwei, JIA Yonghong, ZHANG Jinshan, WANG Kai, LI Dandan, WANG Runqi, DONG Yanxue, SHI Shubing. Effects of drip irrigation capillary spacing and emitter spacing on the spatial distribution of soil water, root morphology and yield of uniformly sown winter wheat [J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1344-1352.
[12]	WANG Guanyu, JIA Pingping, JIN Juan, Abudoukayoumu Ayimaiti, FAN Dingyu, ZHAO Xiaomei, HAO Qing, YANG Lei, GENG Wenjuan. Effects of high temperature stress on physiological characteristics on jujube flower organs [J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1485-1491.
[13]	YANG Mengqi, NAN Shanshan, ZHANG Xiaoyang, WANG Hailiang, LI Jiacheng, NIU Junli, NIE Cunxi, ZHANG Wenju. Effects of fermented cotton meal fiber on growth performance, slaughter performance and intestinal morphology of yellow feather broilers [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 1011-1019.
[14]	LI Shuang, XIE Danlu, WU Jizhou, MA Rui, XIE Aidi, SHAO Lei, GAO Juanjuan, HAN Haixia. Comparison of main functional components and antioxidant activity of jujube Ruoqiang by different processing methods [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 935-942.
[15]	YANG ZHi, DONG Mengyi, WANG Zhenlei, YAN Fenfen, WU Cuiyun, WANG Jiurui, LIU Mengjun, LIN Minjuan. TPA Method was Used to Analyze the Texture and Split of F₁ Fruit of Jujube Hybrid [J]. Xinjiang Agricultural Sciences, 2023, 60(3): 608-615.