不同硅处理方式对干旱胁迫下大麦萌发的影响

doi:10.6048/j.issn.1001-4330.2023.02.008

摘要/Abstract

摘要：

【目的】 研究不同处理方式下不同浓度的硅对干旱胁迫下大麦萌发的影响,综合评价各处理下大麦幼苗的抗旱性,确定最佳处理。【方法】 分别在种子发芽前使用硅溶液浸种(A处理)和在种子发芽的培养过程中向发芽盒里加硅培养液(B处理)两种方式处理新啤6号大麦种子,两种方式均设置0.0、0.5、1.0、1.5和2.0 mmol/L Na₂SiO₃·9H₂O浓度水平,研究对干旱胁迫下大麦发芽指标、形态指标和生长量的影响。【结果】 A处理下随着硅浓度的增加发芽势、发芽指数、活力指数、根长、芽长、芽鞘长、根数、根和芽的鲜重与干重均呈现先增加后降低的趋势,而根芽比呈先降低后增加的趋势,发芽率无显著变化。B处理下0.0和0.5 mmol/L Na₂SiO₃·9H₂O浓度下各指标均无显著差异,1.0~2.0浓度区间内根芽比随硅浓度增加呈降低趋势,其他各指标随硅浓度增加而增加。【结论】 1.0 mmol/L Na₂SiO₃·9H₂O浸种处理的大麦幼苗抗旱性最佳。

关键词: 硅; 干旱胁迫; 大麦(Hordeum Vulgare L.); 萌发

Abstract:

【Objective】 To study the effects of different concentrations of silicon on the germination of barley under drought stress with different treatments and comprehensively evaluate the drought resistance of barley seedlings under each treatment, so as to determine the best treatment. 【Methods】 Barley variety 'Xinpi6' was treated respectively by soaking seeds with silicon solution before germination (treatment A) and adding silicon culture solution to the germination box (treatment B).The concentration of Na₂SiO₃·9H₂O was set at 0.0, 0.5, 1.0, 1.5 and 2.0 mmol/L in both methods to study the effects of drought stress on germination indicator, morphological indicator and growth of barley. 【Result】 In the treatment A, with the increase of silicon concentration, germination potential, germination index, vigor index, root length, bud length, coleophyllum length, root number, fresh and dry weight of root and bud all increased first and then decreased, while the root-bud ratio decreased first and then increased.There was no significant difference in germination rate under different silicon concentrations.In the treatment B, there was no significant difference in all indicators at 0.0 and 0.5 mmol/L Na₂SiO₃·9H₂O concentrations.The root shoot ratio decreased with the increase of the silicon concentration from 1.0 to 2.0, while other indicators increased with the increase of silicon concentration. 【Conclusion】 It was the best for the drought resistance of barley seedlings treated by soaking of seeds with 1.0 mmol/L Na₂SiO₃·9H₂O.

Key words: silicon; drought stress; barley (Hordeum Vulgare L.); germination

中图分类号:

S512.3

宋凌宇, 齐军仓, 张松, 邱英, 尚纪霏, 冯彩军. 不同硅处理方式对干旱胁迫下大麦萌发的影响[J]. 新疆农业科学, 2023, 60(2): 317-325.

SONG Lingyu, QI Juncang, ZHANG Song, QIU Ying, SHANG Jifei, FENG Caijun. Effects of Different Silicon Treatments on Barley Germination under Drought Stress[J]. Xinjiang Agricultural Sciences, 2023, 60(2): 317-325.

图/表 8

图1 不同硅处理方式下大麦种子每日的发芽变化

Fig.1 Daily germination of barley seeds under different silicon treatments

图2 硅处理干旱胁迫下大麦发芽势、发芽率和发芽指数变化

Fig.2 Effects of silicon treatment on germination potential, germination rate and germination index of barley under drought stress

表1 硅处理干旱胁迫下大麦幼苗根长、芽长、芽鞘长和根数变化

Table 1 Effects of silicon treatment on root length, bud length, bud sheath length and root number of barley seedlings under drought stress

处理 Treatment	Na₂SiO₃·9H₂O浓度 Na₂SiO₃·9H₂O concentration (mmol/L)	根长 Root length (cm)	芽长 Bud length (cm)	芽鞘长 Coleophyllum length(cm)	根数 Root number (条)
	CK₁	2.38±0.35^c	0.85±0.22^c	0.85±0.22^d	4.93±0.12^c
	0.5	2.61±0.03^c	1.20±0.14^ab	1.26±0.07^b	5.13±0.12^ab
A	1.0	3.43±0.37^a	1.33±0.07^a	1.69±0.24^a	5.27±0.12^a
	1.5	3.11±0.10^ab	0.99±0.06^bc	1.13±0.03^bc	4.87±0.12^c
	2.0	2.80±0.21^bc	0.88±0.00^c	0.94±0.04^cd	5.00±0.12^bc
	CK₂	2.21±0.07^cd	0.82±0.04^c	0.83±0.05^d	4.93±0.24^a
	0.5	2.14±0.15^d	0.78±0.10^c	0.86±0.02^d	4.97±0.11^a
B	1.0	2.30±0.07^c	0.95±0.07^c	1.02±0.05^c	5.07±0.12^a
	1.5	2.70±0.25^b	1.16±0.03^b	1.20±0.03^b	5.17±0.23^a
	2.0	3.07±0.24^a	1.27±0.14^a	1.50±0.12^a	5.07±0.12^a

表2 硅处理干旱胁迫下大麦幼苗根和芽重量变化

Table 2 Effects of silicon treatment on root and bud weight of barley seedlings under drought stress

处理 Treat- ment	Na₂SiO₃·9H₂O 浓度 Na₂SiO₃·9H₂O concentration (mmol/L)	根鲜重 Root fresh weight (g)	芽鲜重 Bud fresh weight (g)	根干重 Root dry weight (g)	芽干重 Bud dry weight (g)	根芽比 Root bud ratio
	CK₁	0.462 5±0.038 5^c	0.121 2±0.022 4^c	0.094 0±0.007 7^c	0.034 1±0.005 1^b	2.755 6±0.205 8^a
	0.5	0.537 2±0.038 0^b	0.150 8±0.003 7^b	0.111 6±0.006 ${0^{a}}^{b}$	0.041 6±0.002 ${5^{a}}^{b}$	2.684 0±0.027 5^a
A	1.0	0.644 2±0.031 9^a	0.260 1±0.019 9^a	0.122 7±0.005 9^a	0.062 2±0.005 5^a	1.972 7±0.080 4^c
	1.5	0.526 6±0.048 ${6^{b}}^{c}$	0.165 3±0.004 8^b	0.104 7±0.004 ${6^{b}}^{c}$	0.045 5±0.000 ${6^{a}}^{b}$	2.299 4±0.100 4^b
	2.0	0.475 9±0.045 ${5^{b}}^{c}$	0.145 7±0.022 5^b	0.110 2±0.008 5^b	0.041 0±0.003 ${2^{a}}^{b}$	2.687 0±0.052 7^a
	CK₂	0.448 8±0.032 9^c	0.120 0±0.017 8^d	0.092 6±0.002 2^c	0.034 1±0.000 5^d	2.715 5±0.058 5^a
	0.5	0.435 5±0.018 6^c	0.115 0±0.013 9^d	0.090 4±0.000 9^c	0.033 1±0.001 5^d	2.728 3±0.137 8^a
B	1.0	0.475 6±0.017 ${5^{b}}^{c}$	0.142 7±0.008 8^c	0.093 4±0.002 6^c	0.039 4±0.001 7^c	2.373 1±0.045 3^b
	1.5	0.521 8±0.059 ${8^{a}}^{b}$	0.194 1±0.017 9^b	0.103 9±0.004 3^b	0.046 8±0.002 2^b	2.218 0±0.088 6^b
	2.0	0.567 4±0.044 3^a	0.238 7±0.024 1^a	0.112 7±0.004 9^a	0.056 8±0.003 5^a	1.982 3±0.046 8^c

表2 硅处理干旱胁迫下大麦幼苗根和芽重量变化

Table 2 Effects of silicon treatment on root and bud weight of barley seedlings under drought stress

处理 Treat- ment	Na₂SiO₃·9H₂O 浓度 Na₂SiO₃·9H₂O concentration (mmol/L)	根鲜重 Root fresh weight (g)	芽鲜重 Bud fresh weight (g)	根干重 Root dry weight (g)	芽干重 Bud dry weight (g)	根芽比 Root bud ratio
	CK₁	0.462 5±0.038 5^c	0.121 2±0.022 4^c	0.094 0±0.007 7^c	0.034 1±0.005 1^b	2.755 6±0.205 8^a
	0.5	0.537 2±0.038 0^b	0.150 8±0.003 7^b	0.111 6±0.006 ${0^{a}}^{b}$	0.041 6±0.002 ${5^{a}}^{b}$	2.684 0±0.027 5^a
A	1.0	0.644 2±0.031 9^a	0.260 1±0.019 9^a	0.122 7±0.005 9^a	0.062 2±0.005 5^a	1.972 7±0.080 4^c
	1.5	0.526 6±0.048 ${6^{b}}^{c}$	0.165 3±0.004 8^b	0.104 7±0.004 ${6^{b}}^{c}$	0.045 5±0.000 ${6^{a}}^{b}$	2.299 4±0.100 4^b
	2.0	0.475 9±0.045 ${5^{b}}^{c}$	0.145 7±0.022 5^b	0.110 2±0.008 5^b	0.041 0±0.003 ${2^{a}}^{b}$	2.687 0±0.052 7^a
	CK₂	0.448 8±0.032 9^c	0.120 0±0.017 8^d	0.092 6±0.002 2^c	0.034 1±0.000 5^d	2.715 5±0.058 5^a
	0.5	0.435 5±0.018 6^c	0.115 0±0.013 9^d	0.090 4±0.000 9^c	0.033 1±0.001 5^d	2.728 3±0.137 8^a
B	1.0	0.475 6±0.017 ${5^{b}}^{c}$	0.142 7±0.008 8^c	0.093 4±0.002 6^c	0.039 4±0.001 7^c	2.373 1±0.045 3^b
	1.5	0.521 8±0.059 ${8^{a}}^{b}$	0.194 1±0.017 9^b	0.103 9±0.004 3^b	0.046 8±0.002 2^b	2.218 0±0.088 6^b
	2.0	0.567 4±0.044 3^a	0.238 7±0.024 1^a	0.112 7±0.004 9^a	0.056 8±0.003 5^a	1.982 3±0.046 8^c

表3 硅处理下大麦幼苗主成分列表及方差贡献率

Table 3 Component list and percentage of variance of barley seedlings under silicon treatment

处理 Treatment	主成分 Component	特征值 Eigenvalue	方差贡献率 Percentage of variance(%)	累积方差贡献率 Cumulative percentage(%)
A	1	11.398	87.678	88.147
	2	1.070	8.234	95.912
B	1	12.270	94.384	94.384
	2	0.545 26	4.194	98.579

表4 硅处理下大麦幼苗主成分因子载荷矩阵

Table 4 Component factor load matrix of barley seedlings under silicon treatment

处理 Treatment	发芽指标 Germination indicator	因子载荷 Factor load		形态指标 Morphological indicator	因子载荷 Factor load		生长量 Growth	因子载荷 Factor load
处理 Treatment	发芽指标 Germination indicator	1	2	形态指标 Morphological indicator	1	2	生长量 Growth	1	2
	发芽势	0.915	-0.329	根长	0.885	-0.450	根鲜重	0.982	0.046
	发芽率	0.946	0.249	根数	0.799	0.568	芽鲜重	0.976	-0.099
A	发芽指数	0.982	-0.001	芽长	0.900	0.344	根干重	0.916	0.198
	活力指数	0.993	-0.052	芽鞘长	0.979	0.130	芽干重	0.984	-0.142
							根芽比	-0.894	0.408
	发芽势	0.991		根长	0.985		根鲜重	0.998
	发芽率	0.954		根数	0.742		芽鲜重	0.995
B	发芽指数	1.000		芽长	0.997		根干重	0.976
	活力指数	0.998		芽鞘长	0.986		芽干重	0.993
							根芽比	-0.985

表5 硅处理下大麦幼苗抗旱能力的主成分综合评价

Table 5 Comprehensive evaluation on drought resistance ability of barley seedlings under silicon treatment

处理 Treatment	Na₂SiO₃·9H₂O浓度 Na₂SiO₃·9H₂O concentration(mmol/L)	因子得分Factor score		综合效应指数 Comprehensive effect index
处理 Treatment	Na₂SiO₃·9H₂O浓度 Na₂SiO₃·9H₂O concentration(mmol/L)	1	2	综合效应指数 Comprehensive effect index
	CK₁	-3.600 3	0.005 1	-3.156 2
	0.5	0.183 9	1.387 3	0.275 5
A	1.0	5.010 8	0.038 9	4.396 5
	1.5	-0.289 8	-1.147 7	-0.348 6
	2.0	-1.304 6	-0.283 7	-1.167 2
	CK₂	-2.977 1		-2.977 1
	0.5	-3.386 9		-3.386 9
B	1.0	-0.688 2		-0.688 2
	1.5	2.318 8		2.318 8
	2.0	4.733 4		4.733 4

表6 硅处理下大麦幼苗抗旱能力的隶属函数及综合评价

Table 6 Subordination function and comprehensive evaluation of drought resistance of barley seedlings under silicon treatment

处理 Treatment	Na₂SiO₃· 9H₂O 浓度 Na₂SiO₃· 9H₂O concentration (mmol/L)	发芽势 Germin ation potential R	发芽率 Germin ation rate R	发芽指数 Germin ation index R	活力指数 Vigor index R	根长 Root length R	芽长 Bud length R	芽鞘长 Coleop hyllum length R	根数 Root number R	根鲜重 Root fresh weight R	芽鲜重 Bud fresh weight R	根干重 Root dry weight R	芽干重 Bud dry weight R	根芽比 Root bud ratio R	R	排名 Ranking
	CK₁	0.19	0.12	0.02	0.17	0.13	0.04	0.11	0.03	1.00	0.06	0.42	0.13	0.09	0.19	8
	0.5	0.37	0.76	0.50	0.67	0.49	0.25	0.65	0.29	0.91	0.42	0.75	0.58	0.42	0.54	3
A	1.0	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	0.00	1.00	1.00	1.00	1.00	0.92	1
	1.5	0.75	0.37	0.35	0.00	0.44	0.35	0.44	0.43	0.42	0.71	0.58	0.67	0.47	0.46	5
	2.0	0.51	0.18	0.13	0.33	0.19	0.21	0.61	0.27	0.91	0.52	0.67	0.54	0.31	0.41	6
	CK₂	0.06	0.07	0.00	0.16	0.06	0.03	0.07	0.03	0.95	0.00	0.25	0.04	0.04	0.14	9
	0.5	0.00	0.00	0.04	0.25	0.00	0.00	0.00	0.00	0.97	0.06	0.00	0.00	0.00	0.10	10
B	1.0	0.13	0.31	0.23	0.50	0.19	0.19	0.09	0.21	0.51	0.29	0.42	0.23	0.17	0.27	7
	1.5	0.44	0.69	0.43	0.75	0.41	0.54	0.42	0.47	0.31	0.50	0.58	0.48	0.44	0.50	4
	2.0	0.72	0.89	0.78	0.50	0.63	0.85	0.69	0.82	0.01	0.77	0.75	0.67	0.64	0.67	2

参考文献 17

[1]	徐银萍, 潘永东, 刘强德, 等. 大麦种质资源成株期抗旱性鉴定及抗旱指标筛选[J]. 作物学报, 2020, 46(3): 448-461. DOI
	XU Yinping, PAN Yongdong, LIU Qiangde, et al. Identification of drought resistance and screening of drought resistance indexes of barley germplasm resources at mature stage[J]. Acta Agronomica Sinica, 2020, 46(3): 448-461. DOI
[2]	鞠乐, 齐军仓, 贺雪, 等. 大麦种子萌发期抗旱性鉴定指标的筛选及抗旱性评价[J]. 新疆农业科学, 2016, 53(11): 2008-2014.
	JU Le, QI Juncang, HE Xue, et al. Screening and evaluation of drought resistance of barley seeds during germination[J]. Xinjiang Agricultural Sciences, 2016, 53(11): 2008-2014.
[3]	任毅, 王仙, 张金汕, 等. 中亚大麦品种萌发期抗旱性筛选与鉴定[J]. 新疆农业科学, 2019, 56(5): 882-889. DOI
	REN Yi, WANG Xian, ZHANG Jincan, et al. Screening and identification of drought resistance of Central Asian barley varieties during germination[J]. Xinjiang Agricultural Sciences, 2019, 56(5): 882-889. DOI
[4]	谭琳元, 李先德. 基于贸易视角的中国大麦产业安全分析[J]. 中国农业资源与区划, 2020, 41(4): 117-123.
	TAN Linyuan, LI Xiande. Safety analysis of Chinese barley industry based on trade perspective[J]. Chinese Journal of Agricultural Resources and Regional Planning, 2020, 41(4): 117-123.
[5]	陈花, 王建军. 硅对荞麦种子萌发及幼苗生长发育的影响[J]. 陕西农业科学, 2016. 62(3): 5-9,21.
	CHEN Hua, WANG Jianjun. Effect of silicon on seed germination and seedling growth and development of buckwheat[J]. Shaanxi Journal of Agricultural Sciences, 2016, 62(3): 5-9,21.
[6]	赵培培, 赵长江, 于立河, 等. 低温下硅对春小麦种子萌发及抗氧化和渗透调节系统的影响[J]. 麦类作物学报, 2014, 34(6): 823-831.
	ZHAO Peipei, ZHAO Changjiang, YU lihe, et al. Effects of silicon on seed germination, antioxidant and osmotic regulation system of spring wheat at low temperature[J]. Journal of Triticeae Crops, 2014, 34(6): 823-831.
[7]	姚东伟, 吴凌云, 沈海斌, 等. 种子引发技术研究与应用进展[J]. 上海农业学报, 2020. 36(5): 153-160.
	YAO Dongwei, WU Lingyu, SHEN Haibin, et al. Progress in research and application of seed initiation technology[J]. Acta Agriculturae Shanghai, 2020, 36(5): 153-160.
[8]	朱迎树, 齐军仓, 宋瑞娇, 等. 引发对老化大麦籽粒吸水量及活力的影响[J]. 种子, 2018, 37(12): 49-55.
	ZHU Yingchun, QI Juncang, SONG Ruijiao, et al. Effects of initiation on water absorption and vigor of aging barley grains[J]. Seed, 2018, 37(12): 49-55.
[9]	Chiba Y, Mitani N, Yamaji N, et al. HvLsi1 is a silicon influx transporter in barley[J]. The Plant Journal, 2009,(57):810-818.
[10]	Mitani N, Chiba Y, Yamaji N, et al. Identification and characterization of maize and barley Lsi2-like silicon efflux transporters reveals a distinct silicon uptake system from that in rice[J]. The Plant Cell, 2009,(21): 2133-2142.
[11]	杜培兵, 张永福, 白小东, 等. 主成分分析和隶属函数法对马铃薯品种抗旱性的评价[J]. 种子, 2019, 38(8): 120-126.
	DU Peibing, ZHANG Yongfu, BAI Xiaodong, et al. Evaluation of drought resistance of potato varieties by principal component analysis and membership function method[J]. Seed, 2019, 38(8): 120-126.
[12]	字学娟, 杨石有, 李茂. 应用隶属函数法综合评价链荚豆种质抗旱性[J]. 福建农业学报, 2016, 31(8): 844-848.
	ZI Xuejuan, YANG Shiyou, LI Mao. The membership function method was used to evaluate drought resistance of germplasm of Pod bean[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 844-848.
[13]	董文科, 马晖玲, 马婷燕. 外源硅对逆境胁迫下多年生黑麦草种子萌发和幼苗抗性的影响[J]. 甘肃农业大学学报, 2017, 52(6): 90-96.
	DONG Weike, MA Huiling, MA Tingyan. Effects of exogenous silicon on seed germination and seedling resistance of perennial ryegrass under stress[J]. Journal of Gansu Agricultural University, 2017, 52(6): 90-96.
[14]	李玉梅, 杜秋红, 于洪久, 等. 稻壳灰提取硅对水稻种子萌发的影响初探[J]. 华北农学报, 2012, 27(S1): 198-201. DOI
	LI Yumei, DU Qiuhong, YU Hongjiu, et al. Effect of silicon extraction from rice husk ash on seed germination of rice[J]. Acta Agriculturae Boreali-Sinica, 2012, 27(S1): 198-201.
[15]	刘慧霞, 申晓蓉, 郭正刚. 硅对紫花苜蓿种子萌发及幼苗生长发育的影响[J]. 草业学报, 2011, 20(1): 155-160.
	LIU Huixia, SHEN Xiaorong, GUO Zhenggang. Effects of silicon on seed germination and seedling growth and development of Alfalfa[J]. Acta Prataculturae Sinica, 2011, 20(1): 155-160.
[16]	武仲兰, 夏书珍, 何天香, 等. 外源硅对盐胁迫下红豆种子萌发及幼苗生长的影响[J]. 德州学院学报, 2019, 35(6): 34-36,51.
	WU Zhonglan, XIAN Shuzhen, HE Tianxiang, et al. Effects of exogenous silicon on seed germination and seedling growth of adzuki bean under salt stress[J]. Journal of Dezhou University, 2019, 35(6): 34-36,51.
[17]	郭正刚, 王锁民, 田福平, 等. 硅对紫花苜蓿生物学特性的影响[J]. 生态学报, 2006, 26(1): 3302-3307.
	GUO Zhenggang, WANG Suomin, TIAN Fuping, et al. Effect of silicon on biological characteristics of alfalfa[J]. Acta Ecologica Sinica, 2006, 26(1): 3302-3307.

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	5	0	0	49

来源	本网站	其他网站

次数	38	16
比例	70%	30%

摘要

1077

最新录用	在线预览	正式出版

0	0	1077

来源	本网站	其他网站

次数	113	964
比例	10%	90%