根瘤菌接种方式对复播大豆生长及结瘤的影响

doi:10.6048/j.issn.1001-4330.2022.04.004

摘要/Abstract

摘要：

【目的】研究不同根瘤菌接种方式对复播大豆生长及结瘤效果。【方法】以前期分离、鉴定、纯化的3株根瘤菌菌株为材料,对新疆南疆地区复播大豆新大豆8号进行种肥、随水滴施、拌种等接种方式试验,测定其根瘤数、根瘤干重、植株性状、产量及其构成因素的变化。【结果】接种根瘤菌均能促进大豆根系结瘤和生长发育,根瘤菌SN7-2促进效果最明显,其次是SMH12;根瘤菌接种方式中,根瘤菌拌种处理效果最好,其次是随水滴施。其中拌种SN7-2处理组合产量最佳达到3 575.23 kg/hm²,拌种SMH12处理组合产量次之,达到3 376.03 kg/hm²。【结论】较好的根瘤菌和接种方式组合分别是根瘤菌SN7-2和SMH12拌种,其次是根瘤菌SN7-2滴施。

关键词: 根瘤菌; 接种方式; 复播大豆; 结瘤; 生长

Abstract:

【Objective】 To explore the effect of different rhizobia inoculation.【Method】 On the growth and nodulation of the multiple sowing soybean, three rhizobia strains (NW5-3, SN7-2 and SMH12) were used as materials, and three inoculation methods of seed fertilizer, application with drip irrigation and seed dressing were carried out on soybean Xindou 8 in southern Xinjiang, the nodule number, nodule dry weight, plant traits, yield and the yield components were measured.【Result】 Inoculation of rhizobia could promote root nodulation and growth of soybean, and the SN7-2 performed better than the SMH12 and NW5-3.Among the inoculation methods, the seed dressing treatment of rhizobia was the most suitable, followed by application with dripping water.Among them, the optimal yield of the seed dressing SN7-2 treatment combination reached 3,575.23 kg/hm², seed dressing SMH12 treatment combination yield second reached 3,376.03 kg/hm².【Conclusion】 Under the experimental conditions, the most suitable match was the combination of seed dressing with rhizobia SN7-2 and SMH-12, followed by drip application with rhizobia SN7-2.

Key words: rhizobium; inoculation mode; multiple sowing soybean; nodulation; growth

中图分类号:

S565.1
S311

徐玥, 李玲, 胥雅馨, 黄兴军, 吴全忠, 陈国栋, 翟云龙. 根瘤菌接种方式对复播大豆生长及结瘤的影响[J]. 新疆农业科学, 2022, 59(4): 810-817.

XU Yue, LI Ling, XU Yaxin, HUANG Xingjun, WU Quanzhong, CHEN Guodong, ZHAI Yunlong. Effects of Rhizobium Inoculation Methods on Growth and Nodulation of Multiple Sowing Soybeans[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 810-817.

图/表 3

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接种方式 Inoculation method	根瘤菌 Rhizobia	单株瘤数 Number of nodules per plant (个/株)	根瘤鲜重 Nodule fresh weight per plant (g/株)	根瘤体积 Nodule volume per plant (cm³/株)	根瘤干重 Nodule dry weight per plant (g/株)
种肥 Seed manure	NW5-3	19.79^cC	1.68^bBC	1.54^abAB	0.36^bB
	SN7-2	45.62^aA	3.01^aA	3.02^aA	0.73^aA
	SMH12	34.58^bB	2.63^aAB	1.62^abAB	0.42^bB
	CK	9.83^dC	1.52^bC	0.28^bB	0.08^cC
滴施 Drip irrigation	NW5-3	15.02^bB	0.31^cB	0.34^bB	0.14^bBC
	SN7-2	38.13^aA	1.26^bA	1.28^aA	0.36^aAB
	SMH12	43.79^aA	1.73^aA	1.75^aA	0.43^aA
	CK	4.32^cC	0.14^cB	0.13^bB	0.03^bC
拌种 Seed dressing	NW5-3	26.42^bAB	2.32^aA	1.97^bA	0.63^aA
	SN7-2	58.83^aA	2.61^aA	2.36^aA	0.63^aA
	SMH12	32.41^abAB	2.24^aA	2.14^abA	0.54^aA
	CK	6.42^bB	0.29^bB	0.42^cB	0.05^bB

接种方式 Inoculation method	根瘤菌 Rhizobia	单株瘤数 Number of nodules per plant (个/株)	根瘤鲜重 Nodule fresh weight per plant (g/株)	根瘤体积 Nodule volume per plant (cm³/株)	根瘤干重 Nodule dry weight per plant (g/株)
种肥 Seed manure	NW5-3	19.79^cC	1.68^bBC	1.54^abAB	0.36^bB
	SN7-2	45.62^aA	3.01^aA	3.02^aA	0.73^aA
	SMH12	34.58^bB	2.63^aAB	1.62^abAB	0.42^bB
	CK	9.83^dC	1.52^bC	0.28^bB	0.08^cC
滴施 Drip irrigation	NW5-3	15.02^bB	0.31^cB	0.34^bB	0.14^bBC
	SN7-2	38.13^aA	1.26^bA	1.28^aA	0.36^aAB
	SMH12	43.79^aA	1.73^aA	1.75^aA	0.43^aA
	CK	4.32^cC	0.14^cB	0.13^bB	0.03^bC
拌种 Seed dressing	NW5-3	26.42^bAB	2.32^aA	1.97^bA	0.63^aA
	SN7-2	58.83^aA	2.61^aA	2.36^aA	0.63^aA
	SMH12	32.41^abAB	2.24^aA	2.14^abA	0.54^aA
	CK	6.42^bB	0.29^bB	0.42^cB	0.05^bB

接种方式 Inoculation method	根瘤菌 Rhizobia	株高 Plant height (cm)	茎粗 Stem diameter (cm)	主茎节数 Main stalk pitch number (个/株)	单株荚数 Pods number (个/株)
种肥 Seed manure	NW5-3	44.82^abA	0.49^bA	10.78^aA	17.14^bB
	SN7-2	48.26^aA	0.59^aA	10.82^aA	21.23^aA
	SMH12	41.24^bA	0.55^abA	11.23^aA	22.08^aA
	CK	43.62^abA	0.52^abA	11.08^aA	17.42^bB
滴施 Drip irrigation	NW5-3	57.46^bB	0.72^aA	13.14^aA	33.21^aA
	SN7-2	58.16^bB	0.59^bB	11.82^bA	27.83^bB
	SMH12	54.18^bB	0.63^bAB	12.23^abA	21.43^cC
	CK	67.08^aA	0.65^abAB	12.41^abA	17.83^dD
拌种 Seed dressing	NW5-3	70.53^aA	0.62^aA	13.59^aA	24.42^abA
	SN7-2	57.64^bA	0.55^abA	13.24^aA	30.23^aA
	SMH12	68.28^aA	0.53^bA	13.18^aA	25.32^abA
	CK	55.92^bA	0.59^abA	11.63^bA	20.83^bA

接种方式 Inoculation method	根瘤菌 Rhizobia	株高 Plant height (cm)	茎粗 Stem diameter (cm)	主茎节数 Main stalk pitch number (个/株)	单株荚数 Pods number (个/株)
种肥 Seed manure	NW5-3	44.82^abA	0.49^bA	10.78^aA	17.14^bB
	SN7-2	48.26^aA	0.59^aA	10.82^aA	21.23^aA
	SMH12	41.24^bA	0.55^abA	11.23^aA	22.08^aA
	CK	43.62^abA	0.52^abA	11.08^aA	17.42^bB
滴施 Drip irrigation	NW5-3	57.46^bB	0.72^aA	13.14^aA	33.21^aA
	SN7-2	58.16^bB	0.59^bB	11.82^bA	27.83^bB
	SMH12	54.18^bB	0.63^bAB	12.23^abA	21.43^cC
	CK	67.08^aA	0.65^abAB	12.41^abA	17.83^dD
拌种 Seed dressing	NW5-3	70.53^aA	0.62^aA	13.59^aA	24.42^abA
	SN7-2	57.64^bA	0.55^abA	13.24^aA	30.23^aA
	SMH12	68.28^aA	0.53^bA	13.18^aA	25.32^abA
	CK	55.92^bA	0.59^abA	11.63^bA	20.83^bA

接种方式 Inoculation method	根瘤菌 Rhizobia	单株粒数 Grain number per plant (个/株)	百粒重 100-seed weight(g)	产量 Yield(kg/hm²)
种肥 Seed manure	NW5-3	42.23^bB	13.56^bB	1 817.70^bB
	SN7-2	55.42^aA	16.64^aA	2 776.35^aA
	SMH12	55.16^aA	16.61^aA	2 602.05^aA
	CK	40.38^cC	12.03^cB	1 705.65^cC
滴施 Drip irrigation	NW5-3	59.24^cB	15.53^cB	1 904.85^cC
	SN7-2	78.79^aA	19.63^aA	3 210.03^aA
	SMH12	61.41^bB	18.67^bA	2 380.03^bB
	CK	52.62^dC	13.33^dC	1 869.58^dD
拌种 Seed dressing	NW5-3	55.64^cC	22.47^aA	2 591.68^cB
	SN7-2	82.97^aA	21.97^aA	3 575.23^aA
	SMH12	75.24^bB	22.96^aA	3 376.03^bA
	CK	51.31^dD	20.15^bB	2 151.78^dC