新疆农业科学 ›› 2022, Vol. 59 ›› Issue (4): 797-809.DOI: 10.6048/j.issn.1001-4330.2022.04.003
• 作物遗传育种·分子遗传学·耕作栽培·种质资源 • 上一篇 下一篇
李同蕊(), 高振, 王冀川(
), 石元强, 刘强, 周相, 陈雪梅, 王振洋
收稿日期:
2021-07-12
出版日期:
2022-04-20
发布日期:
2022-04-24
通信作者:
王冀川(1968-),男,河北廊坊人,教授,硕士生导师,研究方向为作物高效栽培生理生态,(E-mail) wjcwzy@126.com作者简介:
李同蕊(1997-),女,宁夏银川人,硕士研究生,研究方向为作物生理,(E-mail) 1335801071@qq.com
基金资助:
LI Tongrui(), GAO Zhen, WANG Jichuan(
), SHI Yuanqiang, LIU Qiang, ZHOU Xiang, CHEN Xuemei, WANG Zhenyang
Received:
2021-07-12
Published:
2022-04-20
Online:
2022-04-24
Supported by:
摘要:
【目的】研究灌水量和种植密度对滴灌无膜棉光合特性和产量的影响。【方法】以“中619”为供试材料,设置两因素裂区田间试验,其中灌水量为主区,分别为3 000 m3/hm2(W1)、4 500 m3/ hm2(W2)和 6 000 m3/ hm2 (W3);密度为副区,分别为29.24×104株/hm2(M1)、26.32×104株/hm2(M2)、23.92×104株/hm2(M3)和21.93×104株/hm2(M4),分析无膜棉群体叶面积指数(LAI)动态、光合指标变化及产量差异特征。【结果】盛花期是无膜棉LAI及光合功能最大期,随密度和灌水量增加,LAI增加, W2M3处理的LAI变化较平稳,后期LAI较高,W3M1处理LAI表现最高,但盛花期后下降速度快;适宜灌水及合理密度有利于叶片光合功能提高,其中盛花期W2M3、W2M4和W3M3处理的Pn、Gs和Tr较高,Ci较低,且日变化中的光合“午休”不明显,其最大值分别为29.1032.15 μmolCO2/(m2·s)、0.911.07 mol/(m2·s)、12.7813.87 mmolH2O/(m2·s)和112.1131.0 μmolCO2/(m2·s),产量达6 890.717 496.79 kg/hm2的较高水平。W3M4处理的LAI及光合指标虽然较高,但后期下降过快,而W1M1各项指标均表现最低,灌水及密度过高或过低均不利于叶片光合功能的提高;灌水量与密度对叶片生长及光合指标的影响具有互作效应,且密度效应高于水分效应。【结论】较适宜的灌水量及种植密度范围为4 500 6 000 m3/hm2和21.93×104 23.62×104株/hm2,当灌水量5 034.73 m3/hm2、种植密度24.63×104株/hm2时的产量最高为7 508.82 kg/hm2。
中图分类号:
李同蕊, 高振, 王冀川, 石元强, 刘强, 周相, 陈雪梅, 王振洋. 不同灌水量和密度对无膜棉光合特性及产量的影响[J]. 新疆农业科学, 2022, 59(4): 797-809.
LI Tongrui, GAO Zhen, WANG Jichuan, SHI Yuanqiang, LIU Qiang, ZHOU Xiang, CHEN Xuemei, WANG Zhenyang. Effects of Different Irrigation Amounts and Densities on Photosynthetic Characteristics and Yield of Membrane-Free Cotton[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 797-809.
图1 不同灌水量与密度处理下无膜棉群体LAI动态 注:①横坐标刻度图标的PBS、FBS、IFS、PFS、BS和BOS分别表示初蕾期、盛蕾期、初花期、盛花期、铃期和吐絮期;②同列不同小写字母表示同一灌溉水平下不同密度处理间差异显著(P<0.05)。下同
Fig.1 LAI dynamics of membrane-free cotton population under different irrigation amount and density Note:①PBS, FBS, IFS, PFS, BS and BOS of abscissa scale icon indicate the primary bud stage, full bud stage, initial flowering stage, peak flowering stage, boll stage and boll opening stage, respectively; ②Different small in the same column meant significant difference in different density treatments at the same irrigation level (P<0.05).The same as below
灌水量 Irrigation amount | 密度 Density | 初蕾期 Primary bud stage | 盛蕾期 Full bud stage | 盛花期 Peak flowering stage | 铃期 Bolling stage | 吐絮期 Boll opening stage |
---|---|---|---|---|---|---|
W1 | M1 | 9.50b | 12.31c | 21.41b | 20.49c | 8.50c |
M2 | 10.61a | 13.37c | 24.01ab | 21.00c | 10.22b | |
M3 | 10.85a | 16.61a | 26.76a | 25.97a | 11.84a | |
M4 | 10.39ab | 15.20b | 24.2 | 23.82b | 11.30ab | |
均值 | 10.34 | 14.37 | 24.11 | 22.82 | 10.47 | |
W2 | M1 | 11.64c | 15.05c | 24.91c | 24.09c | 7.08d |
M2 | 12.13c | 16.6 | 27.3 | 27.49b | 9.05c | |
M3 | 15.55a | 20.80a | 32.15a | 30.44a | 15.83a | |
M4 | 13.82b | 17.86b | 29.1 | 27.68b | 13.99b | |
均值 | 13.29 | 17.6 | 28.36 | 27.42 | 11.49 | |
W3 | M1 | 9.84c | 12.43c | 22.77c | 20.97b | 8.93b |
M2 | 10.7 | 14.0 | 25.19b | 24.2 | 8.54b | |
M3 | 12.22a | 18.28a | 29.73a | 28.63a | 10.77a | |
M4 | 11.7 | 15.9 | 26.04b | 25.5 | 6.85c | |
均值 | 11.14 | 15.18 | 25.98 | 24.84 | 8.77 | |
ANOVA检验F值 F value of ANOVA | ||||||
F | W | 69.46** | 19.76** | 203.75** | 15.47** | 68.12** |
M | 13.43* | 145.11** | 403.46** | 19.89** | 15.89* | |
W×M | 14.92** | 18.34** | 16.04** | 10.62** | 32.33** |
表1 不同灌水量与密度下无膜棉Pn变化
Table 1 Effects of different irrigation volume and density on the P n of membrane-free cotton [μmolCO2/(m2·s)]
灌水量 Irrigation amount | 密度 Density | 初蕾期 Primary bud stage | 盛蕾期 Full bud stage | 盛花期 Peak flowering stage | 铃期 Bolling stage | 吐絮期 Boll opening stage |
---|---|---|---|---|---|---|
W1 | M1 | 9.50b | 12.31c | 21.41b | 20.49c | 8.50c |
M2 | 10.61a | 13.37c | 24.01ab | 21.00c | 10.22b | |
M3 | 10.85a | 16.61a | 26.76a | 25.97a | 11.84a | |
M4 | 10.39ab | 15.20b | 24.2 | 23.82b | 11.30ab | |
均值 | 10.34 | 14.37 | 24.11 | 22.82 | 10.47 | |
W2 | M1 | 11.64c | 15.05c | 24.91c | 24.09c | 7.08d |
M2 | 12.13c | 16.6 | 27.3 | 27.49b | 9.05c | |
M3 | 15.55a | 20.80a | 32.15a | 30.44a | 15.83a | |
M4 | 13.82b | 17.86b | 29.1 | 27.68b | 13.99b | |
均值 | 13.29 | 17.6 | 28.36 | 27.42 | 11.49 | |
W3 | M1 | 9.84c | 12.43c | 22.77c | 20.97b | 8.93b |
M2 | 10.7 | 14.0 | 25.19b | 24.2 | 8.54b | |
M3 | 12.22a | 18.28a | 29.73a | 28.63a | 10.77a | |
M4 | 11.7 | 15.9 | 26.04b | 25.5 | 6.85c | |
均值 | 11.14 | 15.18 | 25.98 | 24.84 | 8.77 | |
ANOVA检验F值 F value of ANOVA | ||||||
F | W | 69.46** | 19.76** | 203.75** | 15.47** | 68.12** |
M | 13.43* | 145.11** | 403.46** | 19.89** | 15.89* | |
W×M | 14.92** | 18.34** | 16.04** | 10.62** | 32.33** |
灌水量 Irrigation amount | 密度 Density | 初蕾期 Primary bud stage | 盛蕾期 Full bud stage | 盛花期 Peak flowering stage | 铃期 Bolling stage | 吐絮期 Boll opening stage |
---|---|---|---|---|---|---|
W1 | M1 | 0.27c | 0.32c | 0.49b | 0.32c | 0.1 |
M2 | 0.32b | 0.37c | 0.53b | 0.45b | 0.1 | |
M3 | 0.41a | 0.59a | 0.74a | 0.59a | 0.15a | |
M4 | 0.38a | 0.50b | 0.70a | 0.5 | 0.08c | |
均值 | 0.34 | 0.44 | 0.61 | 0.47 | 0.11 | |
W2 | M1 | 0.34c | 0.41d | 0.66c | 0.49b | 0.08c |
M2 | 0.3 | 0.51c | 0.74c | 0.5 | 0.11b | |
M3 | 0.46a | 0.68a | 1.07a | 0.71a | 0.11b | |
M4 | 0.4 | 0.56b | 0.93b | 0.68a | 0.15a | |
均值 | 0.4 | 0.54 | 0.85 | 0.62 | 0.11 | |
W3 | M1 | 0.28b | 0.41c | 0.54c | 0.43d | 0.13b |
M2 | 0.3 | 0.46c | 0.70b | 0.53c | 0.07c | |
M3 | 0.43a | 0.65a | 0.91a | 0.67a | 0.18a | |
M4 | 0.4 | 0.51b | 0.87a | 0.58b | 0.11b | |
均值 | 0.36 | 0.51 | 0.75 | 0.55 | 0.12 | |
ANOVA检验F值 F value of ANOVA | ||||||
F | W | 15.49** | 102.0** | 105.07** | 31.02** | 39.05** |
M | 10.78* | 15.26* | 286.54** | 15.77* | 3.80ns | |
W×M | 9.19** | 42.23** | 73.28* | 13.46** | 17.84** |
表2 不同灌水量与密度下无膜棉Gs 变化
Table 2 Effects of different irrigation volume and density on the Gs of membrane-free cotton [mol/(m2·s)]
灌水量 Irrigation amount | 密度 Density | 初蕾期 Primary bud stage | 盛蕾期 Full bud stage | 盛花期 Peak flowering stage | 铃期 Bolling stage | 吐絮期 Boll opening stage |
---|---|---|---|---|---|---|
W1 | M1 | 0.27c | 0.32c | 0.49b | 0.32c | 0.1 |
M2 | 0.32b | 0.37c | 0.53b | 0.45b | 0.1 | |
M3 | 0.41a | 0.59a | 0.74a | 0.59a | 0.15a | |
M4 | 0.38a | 0.50b | 0.70a | 0.5 | 0.08c | |
均值 | 0.34 | 0.44 | 0.61 | 0.47 | 0.11 | |
W2 | M1 | 0.34c | 0.41d | 0.66c | 0.49b | 0.08c |
M2 | 0.3 | 0.51c | 0.74c | 0.5 | 0.11b | |
M3 | 0.46a | 0.68a | 1.07a | 0.71a | 0.11b | |
M4 | 0.4 | 0.56b | 0.93b | 0.68a | 0.15a | |
均值 | 0.4 | 0.54 | 0.85 | 0.62 | 0.11 | |
W3 | M1 | 0.28b | 0.41c | 0.54c | 0.43d | 0.13b |
M2 | 0.3 | 0.46c | 0.70b | 0.53c | 0.07c | |
M3 | 0.43a | 0.65a | 0.91a | 0.67a | 0.18a | |
M4 | 0.4 | 0.51b | 0.87a | 0.58b | 0.11b | |
均值 | 0.36 | 0.51 | 0.75 | 0.55 | 0.12 | |
ANOVA检验F值 F value of ANOVA | ||||||
F | W | 15.49** | 102.0** | 105.07** | 31.02** | 39.05** |
M | 10.78* | 15.26* | 286.54** | 15.77* | 3.80ns | |
W×M | 9.19** | 42.23** | 73.28* | 13.46** | 17.84** |
灌水量 Irrigation amount | 密度 Density | 初蕾期 Primary bud stage | 盛蕾期 Full bud stage | 盛花期 Peak flowering stage | 铃期 Bolling stage | 吐絮期 Boll opening stage |
---|---|---|---|---|---|---|
W1 | M1 | 4.05d | 7.13d | 8.23d | 4.24c | 1.08d |
M2 | 5.62c | 8.01c | 9.62c | 5.90b | 2.75c | |
M3 | 8.11a | 9.26a | 11.31b | 6.76a | 3.38a | |
M4 | 6.31b | 8.30b | 10.35a | 6.11b | 2.04b | |
均值 | 6.02 | 8.18 | 9.88 | 5.75 | 2.31 | |
W2 | M1 | 6.43b | 8.16b | 9.70c | 6.26b | 2.67d |
M2 | 7.83a | 8.5 | 12.54b | 6.43b | 3.35c | |
M3 | 7.58a | 10.64a | 13.87a | 8.38a | 4.52a | |
M4 | 6.70b | 8.95c | 13.40a | 6.53b | 3.95b | |
均值 | 7.14 | 9.08 | 12.38 | 6.9 | 3.62 | |
W3 | M1 | 5.80d | 6.78c | 9.04a | 6.31b | 1.66d |
M2 | 6.24c | 8.23b | 11.27b | 5.84b | 2.45c | |
M3 | 8.36a | 10.47a | 12.78a | 7.23a | 3.62a | |
M4 | 7.75b | 8.51b | 11.00b | 6.20b | 2.74b | |
均值 | 7.04 | 8.5 | 11.02 | 6.4 | 2.62 | |
ANOVA检验F值 F value of ANOVA | ||||||
F | W | 5.07* | 27.80** | 32.66** | 6.11* | 22.86** |
M | 2.27ns | 5.89* | 28.81** | 4.31Ns | 20.66** | |
W×M | 134.73** | 34.50** | 134.65** | 43.02** | 201.73** |
表3 不同灌水量与密度下无膜棉 Tr 变化
Table 3 Effects of different irrigation volume and density on the Tr of membrane-free cotton [mmol H 2 O/(m2·s)]
灌水量 Irrigation amount | 密度 Density | 初蕾期 Primary bud stage | 盛蕾期 Full bud stage | 盛花期 Peak flowering stage | 铃期 Bolling stage | 吐絮期 Boll opening stage |
---|---|---|---|---|---|---|
W1 | M1 | 4.05d | 7.13d | 8.23d | 4.24c | 1.08d |
M2 | 5.62c | 8.01c | 9.62c | 5.90b | 2.75c | |
M3 | 8.11a | 9.26a | 11.31b | 6.76a | 3.38a | |
M4 | 6.31b | 8.30b | 10.35a | 6.11b | 2.04b | |
均值 | 6.02 | 8.18 | 9.88 | 5.75 | 2.31 | |
W2 | M1 | 6.43b | 8.16b | 9.70c | 6.26b | 2.67d |
M2 | 7.83a | 8.5 | 12.54b | 6.43b | 3.35c | |
M3 | 7.58a | 10.64a | 13.87a | 8.38a | 4.52a | |
M4 | 6.70b | 8.95c | 13.40a | 6.53b | 3.95b | |
均值 | 7.14 | 9.08 | 12.38 | 6.9 | 3.62 | |
W3 | M1 | 5.80d | 6.78c | 9.04a | 6.31b | 1.66d |
M2 | 6.24c | 8.23b | 11.27b | 5.84b | 2.45c | |
M3 | 8.36a | 10.47a | 12.78a | 7.23a | 3.62a | |
M4 | 7.75b | 8.51b | 11.00b | 6.20b | 2.74b | |
均值 | 7.04 | 8.5 | 11.02 | 6.4 | 2.62 | |
ANOVA检验F值 F value of ANOVA | ||||||
F | W | 5.07* | 27.80** | 32.66** | 6.11* | 22.86** |
M | 2.27ns | 5.89* | 28.81** | 4.31Ns | 20.66** | |
W×M | 134.73** | 34.50** | 134.65** | 43.02** | 201.73** |
灌水量 Irrigation amount | 密度 Density | 初蕾期 Primary bud stage | 盛蕾期 Full bud stage | 盛花期 Peak flowering stage | 铃期 Bolling stage | 吐絮期 Boll opening stage |
---|---|---|---|---|---|---|
W1 | M1 | 333.29a | 243.26a | 207.32a | 211.07a | 274.72a |
M2 | 321.23a | 232.63b | 168.73b | 196.17b | 257.47b | |
M3 | 303.30b | 189.76c | 154.8 | 179.67c | 248.84b | |
M4 | 297.34b | 172.08d | 141.39c | 165.20d | 210.56c | |
均值 | 313.79 | 209.43 | 168.06 | 188.03 | 247.90 | |
W2 | M1 | 343.67a | 232.85a | 191.31a | 194.40a | 248.86a |
M2 | 332.17a | 212.09b | 134.90b | 181.23b | 225.90a | |
M3 | 311.41b | 164.42c | 123.05b | 179.30b | 214.77a | |
M4 | 290.05c | 160.54d | 117.97b | 154.87c | 199.03a | |
均值 | 319.33 | 192.48 | 141.81 | 177.45 | 222.14 | |
W3 | M1 | 340.08a | 239.00a | 167.14a | 190.60a | 265.56a |
M2 | 338.60a | 223.33a | 154.1 | 177.73b | 240.4 | |
M3 | 327.22a | 168.30b | 137.8 | 162.87c | 222.93b | |
M4 | 292.19b | 153.86b | 126.41b | 159.73c | 204.76b | |
均值 | 324.52 | 196.12 | 146.39 | 172.73 | 233.43 | |
ANOVA检验F值 F value of ANOVA | ||||||
F | W | 27.63** | 121.46** | 21.68** | 54.09** | 1.10ns |
M | 17.29* | 4.07ns | 17.04* | 29.58** | 0.63ns | |
W×M | 10.93** | 27.05** | 13.06** | 26.51** | 0.55ns |
表4 不同灌水量与密度下无膜棉Ci变化
Table 4 Effects of different irrigation water and density on the Ci of membrane-free cotton cells (μmolCO2/mol)
灌水量 Irrigation amount | 密度 Density | 初蕾期 Primary bud stage | 盛蕾期 Full bud stage | 盛花期 Peak flowering stage | 铃期 Bolling stage | 吐絮期 Boll opening stage |
---|---|---|---|---|---|---|
W1 | M1 | 333.29a | 243.26a | 207.32a | 211.07a | 274.72a |
M2 | 321.23a | 232.63b | 168.73b | 196.17b | 257.47b | |
M3 | 303.30b | 189.76c | 154.8 | 179.67c | 248.84b | |
M4 | 297.34b | 172.08d | 141.39c | 165.20d | 210.56c | |
均值 | 313.79 | 209.43 | 168.06 | 188.03 | 247.90 | |
W2 | M1 | 343.67a | 232.85a | 191.31a | 194.40a | 248.86a |
M2 | 332.17a | 212.09b | 134.90b | 181.23b | 225.90a | |
M3 | 311.41b | 164.42c | 123.05b | 179.30b | 214.77a | |
M4 | 290.05c | 160.54d | 117.97b | 154.87c | 199.03a | |
均值 | 319.33 | 192.48 | 141.81 | 177.45 | 222.14 | |
W3 | M1 | 340.08a | 239.00a | 167.14a | 190.60a | 265.56a |
M2 | 338.60a | 223.33a | 154.1 | 177.73b | 240.4 | |
M3 | 327.22a | 168.30b | 137.8 | 162.87c | 222.93b | |
M4 | 292.19b | 153.86b | 126.41b | 159.73c | 204.76b | |
均值 | 324.52 | 196.12 | 146.39 | 172.73 | 233.43 | |
ANOVA检验F值 F value of ANOVA | ||||||
F | W | 27.63** | 121.46** | 21.68** | 54.09** | 1.10ns |
M | 17.29* | 4.07ns | 17.04* | 29.58** | 0.63ns | |
W×M | 10.93** | 27.05** | 13.06** | 26.51** | 0.55ns |
灌水量 Irrigation amount | 密度 Density | 单铃重 Boll weight (g) | 单株铃数 Bolls per plant (个/株) | 收获株数 Harvest plant number (104/hm2) | 籽棉产量 Seed cotton yield (kg/hm2) | |||||
---|---|---|---|---|---|---|---|---|---|---|
W1 | M1 | 4.01c | 2.91c | 23.38a | 2 731.35d | |||||
M2 | 4.29b | 3.94b | 21.04b | 3 549.18c | ||||||
M3 | 5.05a | 4.26a | 19.36c | 4 164.55a | ||||||
M4 | 5.12a | 4.38a | 16.87d | 3 779.82b | ||||||
均值 | 4.62 | 3.87 | 20.16 | 3 556.23 | ||||||
W2 | M1 | 4.46b | 6.13c | 23.28a | 6 356.19c | |||||
M2 | 4.8 | 6.90b | 20.65b | 6 836.84b | ||||||
M3 | 5.09a | 7.59a | 19.41b | 7 496.79a | ||||||
M4 | 5.26a | 7.88a | 16.68c | 6 913.66b | ||||||
均值 | 4.90 | 7.13 | 20.01 | 6 900.87 | ||||||
W3 | M1 | 4.18b | 6.13c | 23.16a | 5 932.68c | |||||
M2 | 4.5 | 6.76b | 21.10b | 6 541.62b | ||||||
M3 | 4.99a | 7.10a | 19.44b | 6 890.71a | ||||||
M4 | 4.83b | 7.29a | 17.54c | 6 178.65c | ||||||
均值 | 4.65 | 6.82 | 20.31 | 6 385.92 | ||||||
ANOVA检验F值 F value of ANOVA | ||||||||||
F | W | 2.76ns | 362.11** | 1.84ns | 1 053.15** | |||||
M | 48.85** | 68.07** | 15.76** | 52.17** | ||||||
W×M | 11.51** | 175.62** | 27.38** | 390.14** |
表5 灌水量、密度下产量及其构成因素变化
Table 5 Effects of water volume and density on yield and its composition
灌水量 Irrigation amount | 密度 Density | 单铃重 Boll weight (g) | 单株铃数 Bolls per plant (个/株) | 收获株数 Harvest plant number (104/hm2) | 籽棉产量 Seed cotton yield (kg/hm2) | |||||
---|---|---|---|---|---|---|---|---|---|---|
W1 | M1 | 4.01c | 2.91c | 23.38a | 2 731.35d | |||||
M2 | 4.29b | 3.94b | 21.04b | 3 549.18c | ||||||
M3 | 5.05a | 4.26a | 19.36c | 4 164.55a | ||||||
M4 | 5.12a | 4.38a | 16.87d | 3 779.82b | ||||||
均值 | 4.62 | 3.87 | 20.16 | 3 556.23 | ||||||
W2 | M1 | 4.46b | 6.13c | 23.28a | 6 356.19c | |||||
M2 | 4.8 | 6.90b | 20.65b | 6 836.84b | ||||||
M3 | 5.09a | 7.59a | 19.41b | 7 496.79a | ||||||
M4 | 5.26a | 7.88a | 16.68c | 6 913.66b | ||||||
均值 | 4.90 | 7.13 | 20.01 | 6 900.87 | ||||||
W3 | M1 | 4.18b | 6.13c | 23.16a | 5 932.68c | |||||
M2 | 4.5 | 6.76b | 21.10b | 6 541.62b | ||||||
M3 | 4.99a | 7.10a | 19.44b | 6 890.71a | ||||||
M4 | 4.83b | 7.29a | 17.54c | 6 178.65c | ||||||
均值 | 4.65 | 6.82 | 20.31 | 6 385.92 | ||||||
ANOVA检验F值 F value of ANOVA | ||||||||||
F | W | 2.76ns | 362.11** | 1.84ns | 1 053.15** | |||||
M | 48.85** | 68.07** | 15.76** | 52.17** | ||||||
W×M | 11.51** | 175.62** | 27.38** | 390.14** |
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