Identification and evaluation of salt tolerance in wheat relatives at germination and seedling stages

doi:10.6048/j.issn.1001-4330.2023.05.010

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (5): 1118-1126.DOI: 10.6048/j.issn.1001-4330.2023.05.010

• Crop Genetics and Breeding·Germplasm Resources·Cultivation Physiology·Physiology and Biochemistry • Previous Articles Next Articles

Identification and evaluation of salt tolerance in wheat relatives at germination and seedling stages

Halidan Yikeremu¹(), LIU Na¹, LIU Lianzheng¹, ZHOU Anding¹, JIANG Qiyan², Damailijiang Hezier³, CAO Junmei¹(), ZHANG Xinzhong¹()

1. Institute of Food Crops, Xinjiang Academy of Agricultural Sciences/Northwest wheat Drought and Salt Tolerance Biological Observation Station Ministry of Agriculture, Urumqi 830091, China
2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences,Beijing 100094, China
3. Agricultural Development Service Center of Shuimogou, Fukang Xinjiang 831500, China

Received:2022-07-15 Online:2023-05-20 Published:2023-05-22
Correspondence author: CAO Junmei(1978-),Female,Gansu,associate research fellow,Master,wheat genetics and breeding,(E-mail)caojm0508@126.com;ZHANG Xinzhong(1969-),male,Gansu,Research fellow,Master,The cultivation and promotion direction of new wheat varieties,(E-mail)zxinzhong1031@163.com
Supported by:
Collection and Excellent Gene Tapping of Grain Crop Germplasm Resources(2022A03004-3);Biological Breeding Innovation Projects of Wheat(2021A02001-1);Tianshan Youth Plan(2018Q086)

小麦近缘种芽期和苗期的耐盐性鉴定与评价

哈力旦·依克热木¹(), 刘娜¹, 刘联正¹, 周安定¹, 姜奇彦², 达买力江·合孜尔³, 曹俊梅¹(), 张新忠¹()

1.新疆农业科学院粮食作物研究所/农业部西北地区小麦抗旱耐盐生物学科学观测实验站,乌鲁木齐 830091
2.中国农业科学院作物科学研究所,北京 100094
3.阜康市水磨沟乡农业发展服务中心,新疆昌吉 831500

通讯作者: 曹俊梅(1978-),女,甘肃人,副研究员,硕士,研究方向为小麦遗传育种,(E-mail)caojm0508@126.com; 张新忠(1969-),男,甘肃人,研究员,硕士,研究方向为小麦新品种培育,(E-mail)zxinzhong1031@163.com
作者简介:哈力旦·依克热木(1985-),女,新疆托克逊人,助理研究员,硕士,研究方向为小麦抗逆育种,(E-mail)halida9@163.com
基金资助:
新疆维吾尔自治区重大科技专项“新疆农作物种质资源收集保存与特色资源挖掘利用”子课题:“粮食作物种质资源收集与优异基因挖掘”(2022A03004-3);新疆维吾尔自治区重大科技专项项目“小麦生物育种创新工程”(2021A02001-1);天山青年计划项目(2018Q086)

Abstract

Abstract:

【Objective】 Identification and evaluation of salt tolerance in wheat relatives at germination and seedling stages.【Methods】 In this study, 38 wild relatives of wheat (including controls) were treated at bud and seedling stages.Wheat related species were used as experimental materials and the suitable NaCl concentrations of both 1.2% and 1.5% for salt tolerance identification and evaluation at bud and seedling stage was selected.【Results】 21materials with high salt tolerance at bud stage were obtained(55.2%), accounting for 8 salt-resistant materials(21%) and 3 medium salt-tolerant materials(7.8%);4 for sensitive materials(10.5%),2 for high sensitive(5.2%).7 materials with salt tolerance at seedling stage were obtained(18.4%), 17.medium salt-tolerant materials(44.7%),12 for sensitive materials(31.5%), 2 for high sensitive(5.2%); 2 materials with 100% at the survival rate and seedling rate.At the same time, we also found the same salt-tolerant materials at the bud and seedling stage, including 7 salt-tolerant materials and 4 sensitive materials, and found the survival rate and seedling rate of 100% at both stages for 2 materials.【Conclusion】 The analysis showed that the salt resistance of different salt tolerant types is not consistent in bud stage and seedling stage, and some materials are not comparable and have great differences.But they all have their own significance in the salt-tolerant germplasm screening.

Key words: wheat related materials; bud and seedling stage; screening; identification and evaluation of salt tolerance

摘要：

【目的】鉴定与评价小麦近缘种芽期和苗期的耐盐性。【方法】选用38份(含对照)小麦近缘材料,设芽期、苗期盐胁迫处理,鉴定与评价适宜的芽期和苗期耐盐性NaCl浓度,分析小麦近材料芽期、苗期耐盐性鉴定的NaCl溶液浓度分别为1.2%和1.5%。【结果】获得芽期高耐盐材料21份(占55.2%),耐盐材料8份(占21%),中耐盐材料3份(占7.8%),敏感材料4份(占10.5%),高敏材料2份(占5.2%);苗期耐盐材料7份(占18.4%),中耐盐材料17份(占44.7%),敏感材料12份(占31.5%),高敏材料2份(5.2%);成活率与成苗率均为100%的材料有2份。耐盐材料有7份,敏感材料有4份,成活率与成苗率100%的材料有2份。【结论】不同耐盐类型的材料在芽期和苗期耐盐性不一致,部分材料没有可比性,差异较大。

关键词: 小麦近缘种, 芽期和苗期, 筛选, 耐盐性鉴定与评价

CLC Number:

S512

Halidan Yikeremu, LIU Na, LIU Lianzheng, ZHOU Anding, JIANG Qiyan, Damailijiang Hezier, CAO Junmei, ZHANG Xinzhong. Identification and evaluation of salt tolerance in wheat relatives at germination and seedling stages[J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1118-1126.

哈力旦·依克热木, 刘娜, 刘联正, 周安定, 姜奇彦, 达买力江·合孜尔, 曹俊梅, 张新忠. 小麦近缘种芽期和苗期的耐盐性鉴定与评价[J]. 新疆农业科学, 2023, 60(5): 1118-1126.

Figures/Tables 10

References 17

[1]	刘志勇, 王道文, 张爱民, 等. 小麦育种行业创新现状与发展趋势[J]. 植物遗传资源学报, 2018, 19(3) : 430-434.
	LIU Zhiyong, WANG Daowen, ZHANG Aiming, et al. Innovation status and development trend of wheat breeding[J]. Journal of Plant Genetic Resources, 2018, 19(3) : 430-434
[2]	Jafar M Z, Farooq M, Cheema M A, et al. Improving the performance of wheat by seed priming under saline conditions[J]. Journal of Agronomy and Crop Science, 2012, 198(1) : 38-45. DOI URL
[3]	钮力亚, 王伟伟, 于亮, 等. 抗旱耐盐小麦新品种选育方法[J]. 农业科技通讯, 2021,(3):268-270.
	NIU Yali, WANG Weiwei, YU Liang, et al. Breeding Method of New Wheat Varieties with Drought Resistance and Salt Tolerance[J]. Bulletin of Agricultural Science and Technology, 2021, (3)268-270.
[4]	张志明, 汤才国, 杨三维, 等. 小麦(Triticum aestivum L.)野生资源的发掘、利用研究进展[J]. 生物技术进展, 2016, 6(5):7.
	ZHANG Zhiming, TANG Caiguo, YANG Sanwei, et al. Advances on Gene Discovery and Utilization of Wild Relatives of Triticum aestivum L. Current Biotechnology, 2016, 6(5):7.
[5]	彭智, 李龙, 柳玉平, 等. 小麦芽期和苗期耐盐性综合评价[J]. 植物遗传资源学报, 2017, 18(4):638-645.
	PENG Zhi, LI Long, LIU Yuping, et al. Evaluation of Salinity Tolerance in Wheat(Triticum aestium) Genotypes at Germination and Seedling Stages[J]. Journal of Plant Genetic Resources, 2017, 18(4):638-645
[6]	Wang H L, Chen G L, Zhang H W, et al. Identification of QTLs for salt tolerance at germination and seedling stage of Sorghum bicolor L. Moench[J]. Euphytica, 2013, 196(1) : 117-127 DOI URL
[7]	何晓兰, 徐照龙, 张大勇, 等. 65个高粱种质萌芽期的耐盐指标比较及其耐盐性综合评价[J]. 植物资源与环境学报, 2015, 24(4) : 52-60
	HE Xiaolan, XU Zhaolong, ZHANG Dayong, et al. Comparison on salt tolerance indexes of 65 germplasms of Sorghum bicolor at germination stage and comprehensive evaluation on their salt tolerance[J]. Journal of Plant Resources and Environment, 2015, 24(4) : 52-60
[8]	陈新, 张宗文, 吴斌. 裸燕麦萌发期耐盐性综合评价与耐盐种质筛选[J]. 中国农业科学, 2014, 47 (10) : 2038-2046. DOI
	CHEN Xin, ZHANG Zongwen, WU Bin. Comprehensive Evaluation of Salt Tolerance and Screening for Salt Tolerant Accessions of Naked Oat (Avena nuda L.) at Germination Stage[J]. Scientia Agricultura Sinica, 2014, 47(10) : 2038-2046 DOI
[9]	刘旭, 史娟, 张学勇, 等. 小麦耐盐种质的筛选鉴定和耐盐基因的标记[J]. 植物学报, 2001, 43(9) : 975-948
	LIU Xv, SHI Juan, ZHANG Xueyong, Screening and Identification of Salt-tolerant Germplasm and Marker of Salt-tolerant Gene in Wheat.[J]. Chinese Bulletin of Botany, 2001, 43(9) : 975-948
[10]	董玉琛, 郑殿升. 中国小麦遗传资源[M]. 北京: 中国农业出版社, 2000.
	DONG YUchen, ZHENG Diansheng. Genetic Resources of Wheat in China[M]. Beijing: China Agriculture Press, 2000
[11]	赵贤. 野生二粒小麦抗旱、耐盐种质资源的鉴定及其全基因组关联分析[D]. 杨凌: 西北农林科技大学, 2019.
	ZHAO Xian. Identification of drought and salt-tolerant germplasm resources in wild emmer and genome-wide association analysis[D]. Yangling: North A & F University, 2019.
[12]	杜雅楠. 普通小麦与华山新麦草衍生后代抗旱性初步研究和细胞学鉴定[D]. 杨凌: 西北农林科技大学, 2010.
	DU Yanan. Drought-resistance preliminary research and cytological identification on the progeny of triticum-psathyrostachys[D]. Yangling: North A & F University, 2010.
[13]	曹俊梅, 芦静, 张新忠, 等. 11份新疆小麦品种幼苗耐盐性及相关形态生理特性研究[J]. 新疆农业科学, 2017, 54(8):1384-1393. DOI
	CAO Junmei, LU Jing, ZHANG Xinzhong, et al. Study on Salt Tolerance and Related Morphological and Physiological Characteristics of 11 Xinjiang Wheat Varieties Seedlings[J]. Xinjiang Agricultural Sciences, 2017, 4(8):1384 -1393.
[14]	曹俊梅, 周安定, 吴新元, 等. 盐胁迫对新疆三个冬小麦品种发芽及幼苗期耐盐性研究[J]新疆农业科学, 2010, 47(5) :865-869.
	CAO Jun mei, ZHOU Anding, WU Xinyuan, et al. Effect of Salt Stress on Seed Germination and Seedling Salt -tolerance of Three Winter Wheat Varieties of Xinjiang[J]. Xinjiang Agricultural Sciences, 2010, 47(5):865-869.
[15]	景欣, 张旸, 李玉花. 植物耐盐研究进展[J]. 生物技术通讯, 2010, 21(2):290-294.
	JING Xin,> ZHANG Yang, LI Yuhua. Research progress of plant salt tolerance[J]. Biotechnology Communications, 2010, 21(2):290-294.
[16]	张婷婷, 于崧, 于立河, 松嫩平原春小麦耐盐碱性鉴定及品种(系)筛选[J]. 麦类作物学报, 2016, 36(8):1008-1019.
	ZHANG Tingting, YU Song, YU Lihe, et al. Saline-alkaline tolerance identification and varieties (lines)screening of spring wheat in Songnen plain[J]. Journal of Triticeae Crops, 2016, 36(8):1008-1019.
[17]	Flowers T J, Troke P F, Yeo A R. The mechanism of salt tolerance in halophytes[J]. Plant Physiol, 1977, 28: 89-121.

材料 Material		种类 Species		发芽率 Germination percentage (%)		芽长 Bud length (cm)		根长 Root length (cm)		根数 Root number		芽鲜重 Fresh weight of bud (g)	根鲜重 Root fresh weight (g)
XM884		密穗小麦		99		3.23		3.83		3		0.18	0.08
XM886		密穗小麦		99		2.94		4.27		4		0.21	0.09
XM826		圆锥小麦		99		2.55		3.35		3		0.18	0.14
XM829		圆锥小麦		100		2.87		3.64		4		0.23	0.15
XM712		波斯小麦		99		3.14		4.43		4		0.16	0.07
XM1098		波斯小麦		91		2.70		4.18		4		0.11	0.06
XM16		栽培二粒小麦		99		2.92		3.84		4		0.20	0.11
XM65		栽培二粒小麦		73		3.12		3.02		4		0.15	0.06
XM711-1		栽培二粒小麦		99		3.25		5.09		4		0.23	0.14
XM943		野生一粒小麦		87		3.22		2.77		4		0.11	0.04
XM944		野生一粒小麦		75		4.57		4.29		5		0.15	0.09
XM837		阿拉拉特小麦		98		3.91		5.05		5		0.20	0.15
RM142		高大山羊草		62		3.18		3.80		3		0.08	0.03
XM1107-1		马卡小麦		98		3.23		4.56		4		0.18	0.08
XM836		圆锥小麦		91		2.61		3.36		4		0.17	0.10
XM711		栽培二粒小麦		95		2.09		3.47		4		0.11	0.10
XM838		阿拉拉特小麦		77		4.14		4.17		4		0.13	0.05
RM130		拟斯卑尔脱山羊草		61		3.15		2.91		3		0.08	0.04
RM143		高大山羊草		64		2.69		1.22		3		0.05	0.02
XM10		栽培二粒小麦		87		2.82		3.17		4		0.11	0.08
XM1107		马卡小麦		100		2.61		4.33		4		0.22	0.18
RM103		尾状山羊草		68		2.43		2.83		3		0.04	0.03
RM104		尾状山羊草		77		2.68		2.18		3		0.05	0.03
RM105		尾状山羊草		76		2.60		2.90		3		0.05	0.03
RM126		顶芒山羊草		71		3.42		3.07		3		0.09	0.06
RM50		小亚山羊草		61		2.25		2.06		3		0.06	0.05
RM54	欧山羊草		97		2.93		2.61		3		0.10		0.07
RM078	钩刺山羊草		76		2.91		3.37		5		0.13		0.13
XM883	密穗小麦		99		2.86		3.89		4		0.23		0.12
RM879	密穗小麦		98		2.82		3.78		3		0.14		0.09
XM1106	密穗小麦		99		2.80		4.47		4		0.18		0.14
RM8	卵穗山羊草		84		2.99		2.63		3		0.08		0.03
XM5	野生二粒小麦		80		3.19		3.40		4		0.17		0.09
XM9	栽培二粒小麦		88		2.91		3.12		4		0.42		0.09
XM8	栽培二粒小麦		89		3.94		4.24		5		0.16		0.08
XM708	栽培二粒小麦		82		3.15		2.88		3		0.07		0.05
XM15	栽培二粒小麦		85		3.64		3.38		4		0.16		0.05
新冬34	普通小麦		99		2.92		3.57		4		0.12		0.15

材料 Material		种类 Species		发芽率 Germination percentage (%)		芽长 Bud length (cm)		根长 Root length (cm)		根数 Root number		芽鲜重 Fresh weight of bud (g)	根鲜重 Root fresh weight (g)
XM884		密穗小麦		99		3.23		3.83		3		0.18	0.08
XM886		密穗小麦		99		2.94		4.27		4		0.21	0.09
XM826		圆锥小麦		99		2.55		3.35		3		0.18	0.14
XM829		圆锥小麦		100		2.87		3.64		4		0.23	0.15
XM712		波斯小麦		99		3.14		4.43		4		0.16	0.07
XM1098		波斯小麦		91		2.70		4.18		4		0.11	0.06
XM16		栽培二粒小麦		99		2.92		3.84		4		0.20	0.11
XM65		栽培二粒小麦		73		3.12		3.02		4		0.15	0.06
XM711-1		栽培二粒小麦		99		3.25		5.09		4		0.23	0.14
XM943		野生一粒小麦		87		3.22		2.77		4		0.11	0.04
XM944		野生一粒小麦		75		4.57		4.29		5		0.15	0.09
XM837		阿拉拉特小麦		98		3.91		5.05		5		0.20	0.15
RM142		高大山羊草		62		3.18		3.80		3		0.08	0.03
XM1107-1		马卡小麦		98		3.23		4.56		4		0.18	0.08
XM836		圆锥小麦		91		2.61		3.36		4		0.17	0.10
XM711		栽培二粒小麦		95		2.09		3.47		4		0.11	0.10
XM838		阿拉拉特小麦		77		4.14		4.17		4		0.13	0.05
RM130		拟斯卑尔脱山羊草		61		3.15		2.91		3		0.08	0.04
RM143		高大山羊草		64		2.69		1.22		3		0.05	0.02
XM10		栽培二粒小麦		87		2.82		3.17		4		0.11	0.08
XM1107		马卡小麦		100		2.61		4.33		4		0.22	0.18
RM103		尾状山羊草		68		2.43		2.83		3		0.04	0.03
RM104		尾状山羊草		77		2.68		2.18		3		0.05	0.03
RM105		尾状山羊草		76		2.60		2.90		3		0.05	0.03
RM126		顶芒山羊草		71		3.42		3.07		3		0.09	0.06
RM50		小亚山羊草		61		2.25		2.06		3		0.06	0.05
RM54	欧山羊草		97		2.93		2.61		3		0.10		0.07
RM078	钩刺山羊草		76		2.91		3.37		5		0.13		0.13
XM883	密穗小麦		99		2.86		3.89		4		0.23		0.12
RM879	密穗小麦		98		2.82		3.78		3		0.14		0.09
XM1106	密穗小麦		99		2.80		4.47		4		0.18		0.14
RM8	卵穗山羊草		84		2.99		2.63		3		0.08		0.03
XM5	野生二粒小麦		80		3.19		3.40		4		0.17		0.09
XM9	栽培二粒小麦		88		2.91		3.12		4		0.42		0.09
XM8	栽培二粒小麦		89		3.94		4.24		5		0.16		0.08
XM708	栽培二粒小麦		82		3.15		2.88		3		0.07		0.05
XM15	栽培二粒小麦		85		3.64		3.38		4		0.16		0.05
新冬34	普通小麦		99		2.92		3.57		4		0.12		0.15

处理 Treatment	统计参数 Parameters	发芽率 Germination Percentage (%)
NaCl胁迫	最小值	0.00
NaCl Stress	最大值	100.00
	平均值	66.70
对照	最小值	46.00
Control	最大值	100.00
	平均值	86.00

处理 Treatment	统计参数 Parameters	发芽率 Germination Percentage (%)
NaCl胁迫	最小值	0.00
NaCl Stress	最大值	100.00
	平均值	66.70
对照	最小值	46.00
Control	最大值	100.00
	平均值	86.00

耐盐级别 Salt resistance level	相对盐害率 Relative salt damage rate (%)	耐盐性 Salt tolerance
1级 Level1	[ 0.0,20.0 )	高耐(HT)
2级 Level2	(20.0,40.0]	耐盐(T)
3级 Level3	(40.0,60.0]	中耐(MT)
4级 Level4	(60.0,80.0]	敏感(S)
5级 Level5	(80.0,100.0]	高敏(HS)

Identification and evaluation of salt tolerance in wheat relatives at germination and seedling stages

小麦近缘种芽期和苗期的耐盐性鉴定与评价

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材料 Material	芽期 Germination stage		苗期 Seedling stage
材料 Material	相对盐害率 Relative salt-harmed rate(%)	分级 Grade	盐害指数 Salt- harmed index(%)	分级 Grade
XM884	31.08	2	40.68	2
XM886	11.41	1	35.71	2
XM826	4.73	1	47.49	3
XM829	2.00	1	45.71	3
XM712	14.86	1	66.18	4
XM1098	23.36	2	67.69	4
XM16	0.00	1	52.71	3
XM65	76.70	4	75.75	4
XM711-1	13.42	1	55.72	3
XM943	3.82	1	74.44	4
XM944	67.86	4	82.88	5
XM837	2.04	1	52.89	3
RM142	96.77	5	58.09	3
XM1107-1	20.45	1	46.77	3
XM836	16.18	1	39.40	2
XM711	37.06	2	56.31	3
XM838	1.72	1	63.90	4
RM130	1.10	1	51.71	3
RM143	84.38	5	68.81	4
XM10	1.53	1	78.02	4
XM1107	2.00	1	48.65	3
RM103	41.18	3	60.69	3
RM104	25.00	2	58.62	3
RM105	39.47	2	61.86	4
RM126	38.32	2	60.29	3
RM50	80.22	4	81.21	5
RM54	13.7	1	48.12	3
RM78	57.89	3	57.06	3
XM883	0.00	1	35.57	2
XM879	4.08	1	29.34	2
XM1106	0.00	1	39.00	2
RM8	76.19	4	59.73	3
XM5	33.04	2	61.34	4
XM9	45.9	3	68.00	4
XM8	32.84	2	62.82	4
XM708	17.07	1	56.04	3
XM15	8.59	1	62.61	4
新冬34	0.00	1	29.40	2

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