Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (2): 493-500.DOI: 10.6048/j.issn.1001-4330.2023.02.029
• Forestry·Prataculture·Animal Husbandry Veterinarian • Previous Articles Next Articles
Nasibai Abuduwahapu(), GAO Xiaojuan, Kunduziayi Abudushalamu, LI Jiangwei()
Received:
2022-06-23
Online:
2023-02-20
Published:
2023-03-31
Correspondence author:
LI Jiangwei (1967-), male, Xuchang, Henan province. Ph.D., researcher, master superivisor,(E-mail)jwli67@sina.com
Supported by:
娜斯拜·阿卜杜瓦哈普(), 高晓娟, 坤杜孜阿依·阿布都沙拉木, 李江伟()
通讯作者:
李江伟(1967-),男,新疆乌鲁木齐人,教授,硕士生导师,研究方向为抗体工程及肿瘤免疫治疗,(E-mail)jwli67@sina.com
作者简介:
娜斯拜·阿卜杜瓦哈普(1994-),女,新疆奇台人,硕士研究生,研究方向为生化与分子生物学,(E-mail)1535313706@qq.com
基金资助:
CLC Number:
Nasibai Abuduwahapu, GAO Xiaojuan, Kunduziayi Abudushalamu, LI Jiangwei. Cloning and Sequence Analysis of cDNA of C3d Gene of Bactrian Camel from Xinjiang[J]. Xinjiang Agricultural Sciences, 2023, 60(2): 493-500.
娜斯拜·阿卜杜瓦哈普, 高晓娟, 坤杜孜阿依·阿布都沙拉木, 李江伟. 新疆双峰驼C3d基因cDNA的克隆与序列分析[J]. 新疆农业科学, 2023, 60(2): 493-500.
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引物 Primer | 序列(5'-3') Sequence(5'-3') | C3中位置 Location in the C3 | 产物长度 The length of product(bp) |
---|---|---|---|
C3d-primer-F | cgatccatggTAAAGCACCTCATCGTGACCCCGT | 3 086 | 909 |
C3d-primer-R | attactcgagGCGGCTGGGCAGGTGGATGGAC | 3 995 | 909 |
Table 1 The primers of C3 gene of Bactrian camel in Xinjiang were amplified by PCR
引物 Primer | 序列(5'-3') Sequence(5'-3') | C3中位置 Location in the C3 | 产物长度 The length of product(bp) |
---|---|---|---|
C3d-primer-F | cgatccatggTAAAGCACCTCATCGTGACCCCGT | 3 086 | 909 |
C3d-primer-R | attactcgagGCGGCTGGGCAGGTGGATGGAC | 3 995 | 909 |
Fig.2 Detection of Bactrian camel C3D gene PCR product by gel electrophoresis Note:M:10000 bp DNA marker; 2:1.Negative control; 3:Bactrian camel C3D gene PCR products
Fig.3 identification of pmd18-t-c3d plasmid by Nco I&Xho I double digestion Note:M:10000 bp DNA marker; 1: Single enzyme digestion products of pMD18-T-C3d; 2: Double enzyme digestion products of pMD18-T-C3d
结构分析 Structure analysis | 预测结果 Predicted results |
---|---|
α-螺旋 α-Screw | 12-32 37-44 48-54 110-114 122-140 157-162 176-204 217-227 254-268 273-280 |
β-折叠 β-fold | 1-12 59-93 106-109 163-197 172-175 232-244 276-282 |
T-转角 T-corner | 33-36 44-47 55-58 96-99 102-105 118-121 141-144 146-149 153-156 168-171 205-208 228-231 246-253 269-272 283-286 |
卷曲区 Crimp zone | 1-2 43-45 55-56 58 96-97 99 102 105-106 115-118 145-148 151-153 155-156 168 195 200-201 205 209-210 231-235 240 249-253 268-269 283-284 |
亲水区 Hydrophilic zone | 11-32 33-49 58-60 92-104 105-109 113-126 131-144 145-155 157-160 161-162 163-170 171-172 180-181 183-186 187-189 194-216 230-232 234-235 237-254 263-277 282-283 284-287 |
柔韧性 Flexibility | 14-18 23-27 33-39 43-45 56-59 79-81 93-99 102-106 115-123 136-139 142-157 167-169 183-186 195-210 215-216 227-234 240-253 266-274 |
抗原性 Antigenicity | 12-19 12-37 41-48 55-60 91-107 113-126 135-160 164-172 181-188 189-190 197-213 215-217 227-235 239-254 265-278 283-287 |
表面可及性 Surface accessibility | 11-19 23-28 34-39 43-47 93-98 117-125 165-170 197-212 239-245 264-274 |
Table 2 Analysis of secondary structure of complement C3d of Bactrian camel in Xinjiang
结构分析 Structure analysis | 预测结果 Predicted results |
---|---|
α-螺旋 α-Screw | 12-32 37-44 48-54 110-114 122-140 157-162 176-204 217-227 254-268 273-280 |
β-折叠 β-fold | 1-12 59-93 106-109 163-197 172-175 232-244 276-282 |
T-转角 T-corner | 33-36 44-47 55-58 96-99 102-105 118-121 141-144 146-149 153-156 168-171 205-208 228-231 246-253 269-272 283-286 |
卷曲区 Crimp zone | 1-2 43-45 55-56 58 96-97 99 102 105-106 115-118 145-148 151-153 155-156 168 195 200-201 205 209-210 231-235 240 249-253 268-269 283-284 |
亲水区 Hydrophilic zone | 11-32 33-49 58-60 92-104 105-109 113-126 131-144 145-155 157-160 161-162 163-170 171-172 180-181 183-186 187-189 194-216 230-232 234-235 237-254 263-277 282-283 284-287 |
柔韧性 Flexibility | 14-18 23-27 33-39 43-45 56-59 79-81 93-99 102-106 115-123 136-139 142-157 167-169 183-186 195-210 215-216 227-234 240-253 266-274 |
抗原性 Antigenicity | 12-19 12-37 41-48 55-60 91-107 113-126 135-160 164-172 181-188 189-190 197-213 215-217 227-235 239-254 265-278 283-287 |
表面可及性 Surface accessibility | 11-19 23-28 34-39 43-47 93-98 117-125 165-170 197-212 239-245 264-274 |
物种 Species | 分子量 Molecular weight | 等电点 Isoelectric point | pH7时的电荷 The charge at pH7 | 疏水性平均值 Mean hydrophobicity | 抗原性指数 Antigenicity index |
---|---|---|---|---|---|
新疆双峰驼Xinjiang Bactrian camel | 32 185.02 | 6.4 | -3.13 | 0.13 | 0.53 |
双峰驼Bactrian camel | 33 951.1 | 6.25 | -2.99 | 0.12 | 0.43 |
单峰驼Dromedary camel | 33 951.1 | 6.25 | -2.99 | 0.12 | 0.43 |
羊驼Alpaca | 33 865.05 | 6.7 | -0.99 | 0.1 | 0.39 |
野驼Wild camel | 33 670.65 | 6.03 | -3.99 | 0.07 | 0.44 |
人People | 33 915 | 6.37 | -1.49 | 0.16 | 0.39 |
鼠Rat | 34 015.9 | 5.49 | -6.15 | 0.18 | 0.4 |
猪Pig | 34 160.23 | 6.13 | -3.15 | 0.19 | 0.47 |
家兔Rabbit | 34 255.28 | 7.85 | 1.84 | 0.2 | 0.37 |
牛Cattle | 34 457.36 | 7.38 | 0.84 | 0.36 | 0.5 |
Table 3 Analysis of complement C3d protein of Bactrian camel in Xinjiang
物种 Species | 分子量 Molecular weight | 等电点 Isoelectric point | pH7时的电荷 The charge at pH7 | 疏水性平均值 Mean hydrophobicity | 抗原性指数 Antigenicity index |
---|---|---|---|---|---|
新疆双峰驼Xinjiang Bactrian camel | 32 185.02 | 6.4 | -3.13 | 0.13 | 0.53 |
双峰驼Bactrian camel | 33 951.1 | 6.25 | -2.99 | 0.12 | 0.43 |
单峰驼Dromedary camel | 33 951.1 | 6.25 | -2.99 | 0.12 | 0.43 |
羊驼Alpaca | 33 865.05 | 6.7 | -0.99 | 0.1 | 0.39 |
野驼Wild camel | 33 670.65 | 6.03 | -3.99 | 0.07 | 0.44 |
人People | 33 915 | 6.37 | -1.49 | 0.16 | 0.39 |
鼠Rat | 34 015.9 | 5.49 | -6.15 | 0.18 | 0.4 |
猪Pig | 34 160.23 | 6.13 | -3.15 | 0.19 | 0.47 |
家兔Rabbit | 34 255.28 | 7.85 | 1.84 | 0.2 | 0.37 |
牛Cattle | 34 457.36 | 7.38 | 0.84 | 0.36 | 0.5 |
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