Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (1): 215-222.DOI: 10.6048/j.issn.1001-4330.2022.01.025
• Animal Husbandry Veterinarian·Agricultural Information·Prataculture·Agricultural Eeconomy • Previous Articles Next Articles
LIU Liya(), YE Feng, MA Xiaojing, GU Wenxi, XIE Caiyun, ZHONG Qi, YI Xinping()
Received:
2020-09-30
Online:
2022-01-20
Published:
2022-02-18
Correspondence author:
YI Xinping
Supported by:
刘丽娅(), 叶锋, 马晓菁, 谷文喜, 谢彩云, 钟旗, 易新萍()
通讯作者:
易新萍
作者简介:
刘丽娅(1984-),女,新疆人,副研究员,硕士,研究方向为动物传染病,(E-mail) 286238083@qq.com
基金资助:
CLC Number:
LIU Liya, YE Feng, MA Xiaojing, GU Wenxi, XIE Caiyun, ZHONG Qi, YI Xinping. Analysis of the Difference in Detecting Brucellosis with Different Initial Screening Methods and Different Diagnostic Reagents[J]. Xinjiang Agricultural Sciences, 2022, 59(1): 215-222.
刘丽娅, 叶锋, 马晓菁, 谷文喜, 谢彩云, 钟旗, 易新萍. 布鲁氏菌病不同初筛方法及诊断试剂检测差异性分析[J]. 新疆农业科学, 2022, 59(1): 215-222.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.xjnykx.com/EN/10.6048/j.issn.1001-4330.2022.01.025
项目 Project | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 合计 Total | ||
---|---|---|---|---|
阳性 Positive | 阴性 Negative | |||
4种方法 11种试剂 4 methods 11 reagents | 阳性 | a | b | p1=a+b |
阴性 | c | d | q1=c+d | |
合计 | p2=a+c | q2=b+d | n |
Table 1 Sensitivity、 specificity and consistency coefficient test
项目 Project | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 合计 Total | ||
---|---|---|---|---|
阳性 Positive | 阴性 Negative | |||
4种方法 11种试剂 4 methods 11 reagents | 阳性 | a | b | p1=a+b |
阴性 | c | d | q1=c+d | |
合计 | p2=a+c | q2=b+d | n |
动物 种类 Animal species | 样品 总数 Total number of samples | 检测 试剂 Detection reagent | 检测结果 Test results | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 敏感性 Sensitivity (%) | 特异性 Specificity (%) | 假阳 性率 False positive rate (%) | 假阴 性率 False negative rate (%) | 一致率 Consis tency rate (%) | 英国cELISA 试剂盒 Kappa 值 The Kappa value with the British cELISA Kit | |
---|---|---|---|---|---|---|---|---|---|---|---|
阳性 Positive | 阴性 Negative | ||||||||||
牛 Cattle | 135 | Ⅰ | 阳性 | 71 | 0 | 91 | 100 | 0 | 8.97 | 94.81 | 0.9 |
阴性 | 7 | 57 | |||||||||
Ⅱ | 阳性 | 75 | 0 | 96 | 100 | 0 | 3.85 | 97.78 | 0.95 | ||
阴性 | 3 | 57 | |||||||||
羊 Sheep | 72 | Ⅰ | 阳性 | 30 | 0 | 68 | 100 | 0 | 31.82 | 80.56 | 0.63 |
阴性 | 14 | 28 | |||||||||
Ⅱ | 阳性 | 40 | 0 | 91 | 100 | 0 | 9.09 | 94.44 | 0.89 | ||
阴性 | 4 | 28 |
Table 2 Comparison of differences in detection results of RBT method
动物 种类 Animal species | 样品 总数 Total number of samples | 检测 试剂 Detection reagent | 检测结果 Test results | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 敏感性 Sensitivity (%) | 特异性 Specificity (%) | 假阳 性率 False positive rate (%) | 假阴 性率 False negative rate (%) | 一致率 Consis tency rate (%) | 英国cELISA 试剂盒 Kappa 值 The Kappa value with the British cELISA Kit | |
---|---|---|---|---|---|---|---|---|---|---|---|
阳性 Positive | 阴性 Negative | ||||||||||
牛 Cattle | 135 | Ⅰ | 阳性 | 71 | 0 | 91 | 100 | 0 | 8.97 | 94.81 | 0.9 |
阴性 | 7 | 57 | |||||||||
Ⅱ | 阳性 | 75 | 0 | 96 | 100 | 0 | 3.85 | 97.78 | 0.95 | ||
阴性 | 3 | 57 | |||||||||
羊 Sheep | 72 | Ⅰ | 阳性 | 30 | 0 | 68 | 100 | 0 | 31.82 | 80.56 | 0.63 |
阴性 | 14 | 28 | |||||||||
Ⅱ | 阳性 | 40 | 0 | 91 | 100 | 0 | 9.09 | 94.44 | 0.89 | ||
阴性 | 4 | 28 |
动物 种类 Animal species | 样品 总数 Total number of samples | 检测 试剂 Detection reagent | 检测 结果 Test results | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 敏感性 Sensitivity (%) | 特异性 Specificity (%) | 假阳 性率 False positive rate (%) | 假阴 性率 False negative rate (%) | 一致率 Consis tency rate (%) | 英国cELISA 试剂盒 Kappa 值 The Kappa value with the British cELISA Kit | |
---|---|---|---|---|---|---|---|---|---|---|---|
阳性 Positive | 阴性 Negative | ||||||||||
牛 Cattle | 135 | Ⅲ | 阳性 | 78 | 14 | 100 | 75.44 | 24.56 | 0 | 89.63 | 0.78 |
阴性 | 0 | 43 | |||||||||
羊 Sheep | 72 | Ⅲ | 阳性 | 44 | 8 | 100 | 71.43 | 28.57 | 0 | 88.89 | 0.75 |
阴性 | 0 | 20 |
Table 3 Comparison of differences in FPA detection results
动物 种类 Animal species | 样品 总数 Total number of samples | 检测 试剂 Detection reagent | 检测 结果 Test results | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 敏感性 Sensitivity (%) | 特异性 Specificity (%) | 假阳 性率 False positive rate (%) | 假阴 性率 False negative rate (%) | 一致率 Consis tency rate (%) | 英国cELISA 试剂盒 Kappa 值 The Kappa value with the British cELISA Kit | |
---|---|---|---|---|---|---|---|---|---|---|---|
阳性 Positive | 阴性 Negative | ||||||||||
牛 Cattle | 135 | Ⅲ | 阳性 | 78 | 14 | 100 | 75.44 | 24.56 | 0 | 89.63 | 0.78 |
阴性 | 0 | 43 | |||||||||
羊 Sheep | 72 | Ⅲ | 阳性 | 44 | 8 | 100 | 71.43 | 28.57 | 0 | 88.89 | 0.75 |
阴性 | 0 | 20 |
动物 种类 Animal species | 样品 总数 Total number of samples | 检测 试剂 Detection reagent | 检测 结果 Test results | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 敏感性 Sensitivity (%) | 特异性 Specificity (%) | 假阳 性率 False positive rate (%) | 假阴 性率 False negative rate (%) | 一致率 Consis tency rate (%) | 英国cELISA 试剂盒 Kappa 值 The Kappa value with the British cELISA Kit | |
---|---|---|---|---|---|---|---|---|---|---|---|
阳性 Positive | 阴性 Negative | ||||||||||
牛 Cattle | 135 | A | 阳性 | 78 | 8 | 100 | 85.96 | 14.04 | 0 | 94.07 | 0.88 |
阴性 | 0 | 49 | |||||||||
B | 阳性 | 33 | 0 | 42 | 100.0 | 0 | 57.69 | 66.67 | 0.38 | ||
阴性 | 45 | 57 | |||||||||
C | 阳性 | 78 | 24 | 100 | 57.89 | 42.11 | 0 | 82.22 | 0.61 | ||
阴性 | 0 | 33 | |||||||||
D | 阳性 | 78 | 26 | 100 | 54.39 | 45.61 | 0 | 80.74 | 0.58 | ||
阴性 | 0 | 31 | |||||||||
羊 sheep | 72 | A | 阳性 | 42 | 0 | 95.5 | 100 | 0 | 4.54 | 97.22 | 0.94 |
阴性 | 2 | 28 | |||||||||
B | 阳性 | 24 | 0 | 55 | 100 | 0 | 45.45 | 72.22 | 0.48 | ||
阴性 | 20 | 28 | |||||||||
C | 阳性 | 44 | 8 | 100 | 71.43 | 28.57 | 0 | 88.89 | 0.75 | ||
阴性 | 0 | 20 | |||||||||
D | 阳性 | 44 | 8 | 100 | 71.43 | 28.57 | 0 | 88.89 | 0.75 | ||
阴性 | 0 | 20 |
Table 4 Comparison of differences in detection results of different colloidal gold test strips
动物 种类 Animal species | 样品 总数 Total number of samples | 检测 试剂 Detection reagent | 检测 结果 Test results | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 敏感性 Sensitivity (%) | 特异性 Specificity (%) | 假阳 性率 False positive rate (%) | 假阴 性率 False negative rate (%) | 一致率 Consis tency rate (%) | 英国cELISA 试剂盒 Kappa 值 The Kappa value with the British cELISA Kit | |
---|---|---|---|---|---|---|---|---|---|---|---|
阳性 Positive | 阴性 Negative | ||||||||||
牛 Cattle | 135 | A | 阳性 | 78 | 8 | 100 | 85.96 | 14.04 | 0 | 94.07 | 0.88 |
阴性 | 0 | 49 | |||||||||
B | 阳性 | 33 | 0 | 42 | 100.0 | 0 | 57.69 | 66.67 | 0.38 | ||
阴性 | 45 | 57 | |||||||||
C | 阳性 | 78 | 24 | 100 | 57.89 | 42.11 | 0 | 82.22 | 0.61 | ||
阴性 | 0 | 33 | |||||||||
D | 阳性 | 78 | 26 | 100 | 54.39 | 45.61 | 0 | 80.74 | 0.58 | ||
阴性 | 0 | 31 | |||||||||
羊 sheep | 72 | A | 阳性 | 42 | 0 | 95.5 | 100 | 0 | 4.54 | 97.22 | 0.94 |
阴性 | 2 | 28 | |||||||||
B | 阳性 | 24 | 0 | 55 | 100 | 0 | 45.45 | 72.22 | 0.48 | ||
阴性 | 20 | 28 | |||||||||
C | 阳性 | 44 | 8 | 100 | 71.43 | 28.57 | 0 | 88.89 | 0.75 | ||
阴性 | 0 | 20 | |||||||||
D | 阳性 | 44 | 8 | 100 | 71.43 | 28.57 | 0 | 88.89 | 0.75 | ||
阴性 | 0 | 20 |
动物 种类 Animal species | 样品 总数 Total number of samples | 检测 试剂 Detection reagent | 检测 结果 Test results | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 敏感性 Sensitivity (%) | 特异性 Specificity (%) | 假阳 性率 False positive rate (%) | 假阴 性率 False negative rate (%) | 一致率 Consis tency rate (%) | 英国cELISA 试剂盒 Kappa 值 The Kappa value with the British cELISA Kit | |
---|---|---|---|---|---|---|---|---|---|---|---|
阳性 Positive | 阴性 Negative | ||||||||||
牛 Cattle | 135 | a | 阳性 | 78 | 23 | 100 | 59.65 | 40.35 | 0 | 82.96 | 0.63 |
阴性 | 0 | 34 | |||||||||
b | 阳性 | 78 | 39 | 100 | 31.58 | 68.42 | 0 | 71.11 | 0.35 | ||
阴性 | 0 | 18 | |||||||||
c | 阳性 | 78 | 23 | 100 | 59.65 | 40.35 | 0 | 82.96 | 0.63 | ||
阴性 | 0 | 34 | |||||||||
d | 阳性 | 78 | 26 | 100 | 54.39 | 45.61 | 0 | 80.74 | 0.58 | ||
阴性 | 0 | 31 |
Table 5 Comparison of differences in detection results of different iELISA kits
动物 种类 Animal species | 样品 总数 Total number of samples | 检测 试剂 Detection reagent | 检测 结果 Test results | 英国参考实验室 cELISA试剂盒 UK Reference Laboratory cELISA Kit | 敏感性 Sensitivity (%) | 特异性 Specificity (%) | 假阳 性率 False positive rate (%) | 假阴 性率 False negative rate (%) | 一致率 Consis tency rate (%) | 英国cELISA 试剂盒 Kappa 值 The Kappa value with the British cELISA Kit | |
---|---|---|---|---|---|---|---|---|---|---|---|
阳性 Positive | 阴性 Negative | ||||||||||
牛 Cattle | 135 | a | 阳性 | 78 | 23 | 100 | 59.65 | 40.35 | 0 | 82.96 | 0.63 |
阴性 | 0 | 34 | |||||||||
b | 阳性 | 78 | 39 | 100 | 31.58 | 68.42 | 0 | 71.11 | 0.35 | ||
阴性 | 0 | 18 | |||||||||
c | 阳性 | 78 | 23 | 100 | 59.65 | 40.35 | 0 | 82.96 | 0.63 | ||
阴性 | 0 | 34 | |||||||||
d | 阳性 | 78 | 26 | 100 | 54.39 | 45.61 | 0 | 80.74 | 0.58 | ||
阴性 | 0 | 31 |
[1] | 南京农学院. 家畜传染病学[M]. 北京: 农业出版社, 1982. |
Nanjing Agricultural College. Infectious Diseases of Livestock [M]. Beijing: Agriculture Press, 1982. | |
[2] | 王森. 牛布鲁氏菌病的流行与防控方案[J]. 畜牧兽医科技信息, 2019,(7):86. |
WANG Sen. Epidemic and prevention and control plan of bovine brucellosis[J]. Science and Technology Information of Animal Husbandry and Veterinary Medicine, 2019,(7):86. | |
[3] | 赵贵民, 王洪梅, 何洪彬. 我国家畜布鲁氏菌病流行现状与诊断技术研究进展[J]. 中国畜牧杂志, 2016, 52(16):33-39. |
ZHAO Guimin, WANG Hongmei, HE Hongbin. The epidemiological status of animal brucellosis and research progress of diagnostic technology in our country[J]. Chinese Journal of Animal Science, 2016, 52(16):33-39. | |
[4] | 王功民, 池丽娟, 马世春, 等. 我国畜间布鲁氏菌病流行特点及原因分析[J]. 中国动物检疫, 2010, 27(7):62-63. |
WANG Gongmin, CHI Lijuan, MA Shichun, et al. Epidemiological characteristics and causes of brucellosis among animals in China[J]. Animal Quarantine of China, 2010, 27(7):62-63. | |
[5] | 葛丽萍, 张崇志. 布病的流行现状及防治进展[J]. 当代畜禽养殖业, 2019,(6):3-6. |
GE Liping, ZHANG Chongzhi. The epidemic situation and progress of prevention and treatment of Brucellosis[J]. Contemporary Livestock and Poultry Industry, 2019,(6):3-6. | |
[6] | 莫茜, 阳爱国, 郝力力, 等. 牛羊不同布鲁氏菌病血清学检测方法的比较[J]. 中国动物保健, 2021, 23(8):109-110. |
MO Qian, YANG Aiguo, HAO Lili, et al. Comparison of Different Serological Detection Methods for Brucellosis in Cattle and Sheep[J]. China Animal Health, 2021, 23(8):109-110. | |
[7] | 李盛琼, 侯巍, 莫茜, 等. 不同布鲁氏菌血清学方法检测牛羊血清抗体的比对[J]. 中国动物检疫, 2021, 38(8):99-102. |
LI Shengqiong, HOU Wei, MO Qian, et al. Comparison of Different Brucella Serology Methods for Detection of Bovine and Sheep Serum Antibodies[J]. China Animal Quarantine, 2021, 38(8):99-102. | |
[8] | 蔺旭光, 史量全, 李佳怡, 等. 羊布鲁氏菌病4种血清学检测方法的临床应用比较[J]. 中国微生态学杂志, 2018, 30(4):471-473. |
LIN Xuguang, SHI Liangquan, LI Jiayi, et al. clinical application and comparation of four serological tests for sheep brucellosis in goats[J]. Chinese Journal of Microecology, 2018, 30(4):471-473. | |
[9] | 王慧飞, 宗鹏, 徐磊, 等. Brucellacapt、RBT、SAT、iELISA四种血清学检测方法对布鲁氏菌病检测价值的比较研究[J]. 现代生物医学进展, 2019, 19(4):672-675,641. |
WANG Huifei, ZONG Peng, XU Lei, et al. Comparison of Four Serological Detection Methods of Brucellacapt,RBT, SAT and iELISA in the Detection of Brucellosis[J]. Advances in Modern Biomedicine, 2019, 19(4 ):672-675,641. | |
[10] | 赵娜, 赵赤鸿, 荣蓉, 等. 布鲁氏菌病血清学Brucellacapt和iELISA检测方法的比较[J]. 中国人兽共患病学报, 2014, 30(10):1045-1047,1051. |
ZHAO Na, ZHAO Chihong, RONG Rong, et al. Comparison of Brucellacapt and iELISA in serological diagnosis of Brucellosis[J]. Chinese Journal of Zoonoses, 2014, 30(10 ):1045-1047,1051. | |
[11] | 尉瑞平, 王美霞, 宋利桃, 等. 布鲁氏菌病3种检测方法的比较分析[J]. 医学动物防制, 2019, 35(7):711-713. |
WEI Ruiping, WANG Meixia, SONG Litao, et al. Comparison and analysis of three detection methods for brucellosis[J]. Medical Animal Control, 2019, 35(7):711-713. | |
[12] | 董雅琴, 刘爽, 郑辉, 等. 三种伪狂犬病病毒gE抗体ELISA检测试剂盒的比较[J]. 中国动物检疫, 2017, 34(11):79-81,88. |
DONG Yaqin, LIU Shuang, ZHENG Hui, et al. Comparison of three ELISA kits for gE antibody against pseudorabies virus[J]. China Animal Quarantine, 2017, 34(11):79-81, 88. | |
[13] | 叶尔江·色尔哈孜, 赛力克·库鲁西, 努尔兰·木合买提, 等. 乌鲁木齐市骆驼养殖现状与发展建议[J]. 新疆畜牧业, 2016(8):10-13. |
Yeerjiang Serhazi, Selik Kulushi, Nurlan Mumati, et al. Current status and development suggestions of camel breeding in Urumqi[J]. Xinjiang Animal Husbandry, 2016(8):10-13. | |
[14] | 齐景文, 依颖新, 周艳红, 等. 牛羊布鲁氏菌病四种血清学检测方法对比分析[J]. 中国动物检疫, 2015, 32(4):57-59. |
QI Jingwen, YI Yingxin, ZHOU Yanhong, et al. Comparative analysis of four serological detection methods for cattle and sheep brucellosis[J]. Animal Quarantine of China, 2015, 32(4):57-59. | |
[15] | 朱绍辉, 宫枫举, 邵钰, 等. 两种诊断制品对临床样品中布鲁氏菌抗体的检测比较[J]. 中国动物检疫, 2018, 35(11):92-95. |
ZHU Shaohui, GONG Fengju, SHAO Yu, et al. Comparison of detection of Brucella antibodies in clinical samples by two diagnostic products[J]. China Animal Quarantine, 2018, 35(11):92-95. | |
[16] | 董浩, 韩焘, 徐琦, 等. 3种国产布病抗体检测试剂盒的效果评价[J]. 中国兽医学报, 2020, 40(2):336-338. |
DONG Hao, HAN Tao, XU Qi, et al. Evaluation of three domestic Brucellosis antibody test kits[J]. Chinese Journal of Veterinary Medicine, 2020, 40(2):336-338. | |
[17] | 杨书观, 王爱香. 3种布鲁氏菌病试验方法检测380例布鲁氏菌病结果比较分析[J]. 河南预防医学杂志, 2018, 29(8):598-599,610. |
YANG Shuguan, WANG Aixiang. Comparative analysis of the results of three brucellosis test methods n 380 cases of brucellosis[J]. Henan Preventive Medicine Journal of Science, 2018, 29(8):598-599,610. |
[1] | LAN Chenyihang, YAO Yubo, ZHOU Junxiang, FU Kaiyun, DING Xinhua, YIN Xiaohui, LIU Wen, WANG Na, GUO Wenchao, DENF Jianyu. Research progress on sex pheromone identification and application of Asian corn borer [J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1614-1622. |
[2] | CHEN Yulan, CHENG Xurui, GUO Jun, SU Wuzheng. Regional Difference and Dynamic Evolution of Agricultural Green Development Level in China [J]. Xinjiang Agricultural Sciences, 2023, 60(1): 252-260. |
[3] | XU Mingqiang, MENG Yina, ZHANG Ting, MA Yan, MENG Xintao, ZOU Shuping, ZHANG Qian. Study of Explosion Puffing Drying Process for Xinjiang Thick-Skin Melon Chipsat Pressure Difference [J]. Xinjiang Agricultural Sciences, 2022, 59(6): 1429-1437. |
[4] | WANG Juhua, MENG Jun, WANG Jiangwen, KONG Qisen, YAO Xinkui, WANG Chuankun, JIANG Wendong, YAO Runchen, LIU Yuying, BAO Hongyan. Differential Analysis of Behavior Features of Yili Horses during Different Periods [J]. Xinjiang Agricultural Sciences, 2020, 57(2): 365-374. |
[5] | YAN Jing, MENG Jun, WNAG Jianwen, REN Wanlu , ZHENG Guopeng, LI Yunxia, ZHANG Xin, ZHANG Wen, YUAN Xinxin, YAO Xinkui. Difference Analysis of Meat Edible Quality and Fatty Acid Composition in Different Parts of Yili Horses [J]. Xinjiang Agricultural Sciences, 2020, 57(2): 357-364. |
[6] | WANG Jianwen, YAO Xinkui, CHU Hongzhong, REN Wanlu, WANG Chuankun, MENG Jun. Effects of Training on Blood Gas Indexes of Yili Horse in 1,000 m Speed Race [J]. Xinjiang Agricultural Sciences, 2020, 57(2): 333-339. |
[7] | WANG De;GAO Jiang-sheng;WU Cui-yun;WANG Yan;WANG Zhi-qiang;SUN Ya-qiang. Studies on the Quality Difference of Jujube during Different Bearing Periods [J]. , 2016, 53(2): 254-260. |
[8] | RAN Qi-ying;ZHOU Hui. Regional Differences and Convergence Analysis of Agricultural Total Factor Energy Efficiency in China: 1997-2012 [J]. , 2015, 52(6): 1169-1176. |
[9] | XUE Li-hua;DUAN Li-na;XIE Xiao-qing;CHEN Xing-wu;LEI Jun-jie;Sailihan;QIAO Xu. Analysis of Mechanism of Drip Irrigation Effect on Xindong 18 Interline Differences in Yield [J]. , 2015, 52(4): 614-620. |
[10] | MA Xiao-jing;YI Xin-ping;GU Wen-xi;YE Feng;ZHONG Qi. Cloning and Sequence Analysis of the Promoter SLC11A1 Gene among Three Different Varieties Bovine in Xingjiang [J]. , 2015, 52(11): 2103-2110. |
[11] | CONG Hua;Tatsuya M. Ikeda;Kanenori Takata;Mikiko Yanaka;Hiroshi Fujimaki;Tsukasa Nagamine. Geographical Distribution of High - Molecular - Weight Glutenin Subunits and Characterization of a New High - Molecular - Weight Glutenin Subu nit of Winter Wheat Landraces (Triticum aestivum L.)in the Xinjiang Uygur Autonomous District of China [J]. , 2011, 48(9): 1576-1584. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||