Effects of Three Kinds of Salt Stress on Seed Germination Characteristics of Guisangyou Mulberry 12

doi:10.6048/j.issn.1001-4330.2022.01.024

Abstract

Abstract:

【Objective】 To study the effects of three kinds of salt stress on seed germination of Guisangyou mulberry 12 and determine the tolerance of Guisangyou mulberry 12 to different salt stress,which provides theoretical basis for the introduction and popularization of mulberry in Xinjiang. 【Method】 Guisangyou mulberry 12 seeds were treated with 0.025-0.25 mol/L neutral single salt (NaCl), basic mixed salt (NaCl+Na₂CO₃) and neutral mixed salt (NaCl+Na₂SO₄) respectively. The germination rate, germination potential, germination index, vigor index, proline and malondialdehyde content were measured. Meanwhile, the salt tolerance of Guisangyou mulberry 12 seeds was comprehensively evaluated by the method of subjection function. 【Results】 With the increase of the concentration of the three salts, the germination rate, germination potential, germination index and vigor index of Guisangyou mulberry 12 seeds all decreased, while the contents of proline and malondialdehyde all increased. The regression relationship between seed germination rate and salt concentration was significant in Guisangyou mulberry 12. Under three salt stresses of alkaline mixed salt, neutral single salt and neutral mixed salt, the appropriate germination concentrations of Guisangyou mulberry 12 seeds were 0.043,1 mol/L, 0.040,8 mol/L and 0.036,4 mol/L, respectively, and the salt tolerance limits were 0.252,4 mol/L, 0.295,9 mol/L and 0.290,5 mol/L, respectively. The comprehensive evaluation values of seed germination of Guisangyou mulberry 12 on three salts tresses resistance were 3.565, 3.905 and 5.027, respectively. 【Conclusion】 The stress of three kinds of salt solution on seed germination of Mulberry mulberry 12 is the most serious alkaline mixed salt and the lightest neutral mixed salt.

Key words: Guisangyou mulberry; seed germination; salt stress; mixed salt

摘要：

【目的】研究不同盐胁迫对桂桑优12种子萌发的影响,分析其对不同盐胁迫的耐受性,为新疆桑树引种和推广提供理论依据。【方法】以桑树品种桂桑优12种子为试材,采用0.025~0.25 mol/L的碱性混合盐(NaCl+Na₂CO₃)、中性单盐(NaCl)和中性混合盐(NaCl+Na₂SO₄)胁迫处理种子,测定发芽率、发芽势、发芽指数、活力指数、脯氨酸和丙二醛含量,用隶属函数法综合评价其耐盐性。【结果】随着3种盐溶液浓度的增加,桂桑优12种子发芽率、发芽势、发芽指数、活力指数均呈下降的趋势,而其游离脯氨酸、丙二醛含量均呈上升的趋势;桂桑优12种子发芽率与盐浓度间的回归关系显著。在碱性混合盐、中性单盐和中性混合盐3种盐胁迫下,桂桑优12种子萌发的适宜浓度分别为0.043 1、0.040 8和0.036 4 mol/L,耐盐极限浓度分别为0.252 4 、0.295 9和0.290 5 mol/L;桂桑优12种子萌发对3种盐胁迫抗性的综合评价值分别为:3.565、3.905和5.027。【结论】3种盐溶液对桂桑优12桑树种子萌发的胁迫,最严重为碱性混合盐,最轻的为中性混合盐。

关键词: 桑树, 种子萌发, 盐胁迫, 混合盐

CLC Number:

S888.2

CHEN Jiali, JIANG Xi, TAN Zhanming, YANG Minglu, XIAO Haibing. Effects of Three Kinds of Salt Stress on Seed Germination Characteristics of Guisangyou Mulberry 12[J]. Xinjiang Agricultural Sciences, 2022, 59(1): 205-214.

陈家力, 姜喜, 谭占明, 杨明禄, 肖海兵. 3种盐胁迫对桂桑优12种子萌发特性的影响[J]. 新疆农业科学, 2022, 59(1): 205-214.

Figures/Tables 11

References 34

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浓度梯度 Concen tration gradient	中性单盐 Neutral single salt (mol/L)	碱性混合盐 Alkaline mixed salt (mol/L)	中性混合盐 Neutral mixed salt (mol/L)
浓度梯度 Concen tration gradient	NaCl	NaCl+Na₂CO₃	NaCl+Na₂SO₄
0	0	0	0
1	0.025	0.012 5+0.006 25	0.012 5+0.006 25
2	0.050	0.025+0.012 5	0.025+0.012 5
3	0.100	0.05+0.025	0.05+0.025
4	0.150	0.075+0.037 5	0.075+0.037 5
5	0.200	0.1+0.05	0.1+0.05
6	0.250	0.125+0.062 5	0.125+0.062 5

浓度梯度 Concen tration gradient	中性单盐 Neutral single salt (mol/L)	碱性混合盐 Alkaline mixed salt (mol/L)	中性混合盐 Neutral mixed salt (mol/L)
浓度梯度 Concen tration gradient	NaCl	NaCl+Na₂CO₃	NaCl+Na₂SO₄
0	0	0	0
1	0.025	0.012 5+0.006 25	0.012 5+0.006 25
2	0.050	0.025+0.012 5	0.025+0.012 5
3	0.100	0.05+0.025	0.05+0.025
4	0.150	0.075+0.037 5	0.075+0.037 5
5	0.200	0.1+0.05	0.1+0.05
6	0.250	0.125+0.062 5	0.125+0.062 5

	平均数 Mean±SD	Min	Max	R	CV%	95%置信区间 95% confidence interval
	平均数 Mean±SD	Min	Max	R	CV%	下限 Lower	上限 Upper
长 Length(cm)	0.244±0.016	0.216	0.286	0.070	6.64	0.238	0.250
宽 Width(cm)	0.192±0.019	0.158	0.246	0.088	10.03	0.185	0.199
厚 Thickness(cm)	0.136±0.015	0.104	0.158	0.054	11.00	0.131	0.142
千粒重 Thousand kernel weight(g)	2.169±0.026	2.136	2.216	0.080	1.21	2.159	2.179

	平均数 Mean±SD	Min	Max	R	CV%	95%置信区间 95% confidence interval
	平均数 Mean±SD	Min	Max	R	CV%	下限 Lower	上限 Upper
长 Length(cm)	0.244±0.016	0.216	0.286	0.070	6.64	0.238	0.250
宽 Width(cm)	0.192±0.019	0.158	0.246	0.088	10.03	0.185	0.199
厚 Thickness(cm)	0.136±0.015	0.104	0.158	0.054	11.00	0.131	0.142
千粒重 Thousand kernel weight(g)	2.169±0.026	2.136	2.216	0.080	1.21	2.159	2.179

盐种类Salt type	适宜浓度 Suitable concentration (mol/L)	半致死浓度 Half lethal concentration (mol/L)	耐盐极限 Salt tolerance limi (mol/L)
碱性混合盐Alkaline mixed salt	0.043 1	0.123 6	0.252 4
中性单盐Neutral single salt	0.040 8	0.138 9	0.295 9
中性混合盐Neutral mixed salt	0.036 4	0.134 2	0.290 5