Changes of Endogenous Hormones in Malus sieversii with Different Health Conditions

doi:10.6048/j.issn.1001-4330.2020.03.011

Abstract

Abstract: 【Objective】 To analyze the changes of endogenous hormones during the growth and development of Xinjiang Malus sieversii forest under different health conditions, and to provide scientific basis for the research on the protection of Malus sieversii. 【Method】The contents of IAA, GA₃, ABA, ZR, JA in leaves and fruits of Xinjiang Malus sieversii with different development cycles, different years and different health conditions were determined by high performance liquid chromatography (HPLC). 【Result】From June to September, the contents of ZR and GA₃ in the leaves of normal Malus sieversii decreased, and the contents of JA, IAA and ABA increased. The contents of ZR, JA, GA₃, IAA and ABA in the degraded Malus sieversii increased. The normal fruits ZR, JA, GA₃, and IAA showed the same trend, and all showed a downward trend. Compared with those in 2017, the contents of ZR, JA and ABA in the leaves of normal Xinjiang Malus sieversii increased, and the contents of GA₃ and IAA decreased. The content of ZR and ABA in the leaves of degraded Malus sieversii increased, and the contents of GA₃ and IAA decreased. Normal and degraded endogenous hormone contents of fruits showed the same trend, and the content of JA increased, and the contents of ZR, GA₃, IAA and ABA decreased. Among the five Malus sieversii with different survival rates, the 100% survival rate of the leaves of Malus sieversii forest was the highest in IAA, ZR and GA₃, and the 20% survival rate of Malus sieversii forest leaves was the highest, 10% survival rate of Malus sieversii forest leaves was the highest. It can be seen that the higher the survival rate, the higher the hormone content that promotes growth. 【Conclusion】The contents of five endogenous hormones in Malus sieversii under two different growth conditions showed different trends in different months and years.

Key words: Malus sieversii; degradation; growth potential; endogenous hormones

摘要： 【目的】分析不同生长状况下新疆野苹果野果林在生长发育过程中内源激素的变化规律,为新疆野苹果保护研究提供科学依据。【方法】分别于不同发育周期、不同年限、不同生长状况的新疆野苹果叶片与果实鲜样,采用高效液相法测定IAA、GA₃、ABA、ZR、JA这5种激素的含量。【结果】从6~9月,正常的新疆野苹果叶片ZR、GA₃含量下降,JA、IAA、ABA含量上升;退化的新疆野苹果叶片ZR、JA、GA₃、IAA、ABA含量上升;退化和正常的果实ZR、JA、GA₃、IAA变化趋势相同,均呈下降的趋势。2018年相比于2017年,正常的新疆野苹果叶片ZR、JA、ABA含量上升,GA₃、IAA 含量下降;退化的新疆野苹果叶片ZR、ABA含量上升,GA₃、IAA 含量下降;正常和退化的果实内源激素含量变化趋势相同,均是JA 含量上升,ZR、GA₃、IAA、ABA 含量下降。在5种不同存活率的新疆野苹果中,得出100%存活率新疆野苹果林叶片的IAA、ZR、GA₃含量最高,20%存活率新疆野苹果林叶片的JA含量最高,10%存活率新疆野苹果林叶片的ABA含量最高,得出存活率越高,促进生长的激素含量也会越高。【结论】2种不同生长状况下,新疆野苹果5种内源激素含量在不同月份和不同年份表现出不同趋势。

关键词: 新疆野苹果, 退化, 生长势, 内源激素

CLC Number:

S661.1

WANG Zhendong, CHEN Qiling, ZHENG Qiangqing, WANG Jingjing, WANG Wenjun, CAI Yong. Changes of Endogenous Hormones in Malus sieversii with Different Health Conditions[J]. Xinjiang Agricultural Sciences, 2020, 57(3): 477-275.

王振东, 陈奇凌, 郑强卿, 王晶晶, 王文军, 蔡勇. 不同生长状况下新疆野苹果内源激素含量的变化[J]. 新疆农业科学, 2020, 57(3): 477-275.

References

[1]廖康. 新疆野生果树资源研究[M].乌鲁木齐:新疆科学技术出版社,2013.
LIAO Kang. Research on wild fruit resources in Xinjiang [M]. Urumqi: Xinjiang Science and Technology Press, 2013.
[2]郭仲军,刘丽艳,张炜银,等. 新疆野苹果资源现状分析及前景展望[A].见:全国生物多样性保护与持续利用研讨会主编.中国生物多样性保护与研究进展[C]北京:气象出版社,2004,54-61.
GUO Zhongjun LIU Iiyan,ZHANG Weiyin et al.In: National Symposium on Biodiversity Conservation and Sustainable Utilization .Advances in Biodiversity Conservation and Research in China[C]. Beijing: China Meteorological Press, 2004,54-61.
[3]田小平.新疆野苹果自然保护区现状评价[J].中国林业经济,2016,(3):71-72.
TIAN Xiaoping. Evaluation on the Current Situation of Wild Apple Nature Reserve in Xinjiang [J]. China Forestry Economics, 2016,(3):71-72.
[4]张苹,吕昭智,张鑫,等.新疆伊犁与哈萨克斯坦新疆野苹果[Malus sieversii (Ledeb.)Roem.]种群年龄结构[J].干旱区研究:1-19
ZHANG Ping, LU Zhaoahi,ZHANG Xin, et al. Age Structure of Wild Apple (Malus sieversii (Ledeb.)Roem.) Population in Yili, Xinjiang and Kazakhstan [J]. Arid Zone Research, 2019, 36(4) : 844-853.
[5]马闯,杨美玲,张云秀,等.新疆野苹果(Malus sieversii)种群年龄结构及其动态特征[J].干旱区研究,2018,35(1):156-164.
MA Chuang, YANG Meiling,ZHANG Yunxiu, et al, Age Composition and Dynamic Characteristics of the Main Populations of Endangered Malus sieversii [J]. Arid Zone Research, 2018,35(1):156-164.
[6]田润炜,蔡新斌,刘丽燕,等.新疆野苹果种群年龄结构特征与动态分析[J].西北植物学报,2016,36(4):811-817.
TIAN Runwei, CAI Xinbin, LIU Liyan, et al. Characteristics of the age structure and dynamics of Malus sieversii population [J].Acta Botanica Boreali-Occidentalia Sinica, 2016,36(4) : 811－817.
[7]于玮玮,曹波,龙鸿,等.新疆野苹果幼苗对盐胁迫的生理响应[J].华北农学报, 2016, 31(1): 170-174.
YU Weiwei, CAO Bo, LONG Hong, et al. Physiological Responses of Malus sieversii Seedling Under Salinity Stress [J]. Acta Agriculture Boreali-sinica, 2016, 31(1): 170-174.
[8]谭冬梅.干旱胁迫对新疆野苹果及平邑甜茶生理生化特性的影响[J].中国农业科学, 2007, 40(5): 980-986.
TAN Dongmei. The physiology and biochemistry analyzing of programmed cell death under drought stress in two Malus [J]. Scientia Agricultura Sinica, 2007, 40(5): 980-986.
[9]贾旭梅,朱燕芳,胡亚,等.三种苹果砧木在水培条件下对缺铁胁迫的耐受性(英文)[J]. 农业科学与技术:(英文版),2018,19(3):21-30.
JIA Xumei,ZHU Hyanfang, HU Ya, et al. Tolerance to Iron-Deficiency Stress of Three Apple Rootstock Species in Hydroponic System[J]. Agricultural Science & Technology, 2018,19(3):21-30.
[10]熊国胜,李家洋,王永红.植物激素调控研究进展[J].科学通报, 2009, 54(18): 2718~2733.
XIONG Guosheng S, LI Jiayang, WANG Yonghong. Advances in the regulation and crosswalks of phytohormones [J]. Chinese Science Bulletin, 2009, 54(18): 2718-2733.
[11]王西成,吴伟民,赵密珍,等.激素调控葡萄果实发育与成熟的生理与分子机制研究进展[J].江西农业大学学报,2015,37(40):604-611.
WANG Xichang, WU Weimin M, ZHAO Mizhen Z, et al. Progress in the Research of Physiological and Molecular Mechanism in Development and Ripening of Grape Fruit Regulated by Hormones [J]. Acta Agriculture Universitatis Jiangxiensis, 2015, (4): 604-611.
[12]段娜, 贾玉奎, 徐军,等. 植物内源激素研究进展[J].中国农学通报, 2015, 31(2):159-165.
DUAN Na, JIA Ynhui, XU Jun, et al. Research Progress on Plant Endogenous Hormones [J]. Chinese Agricultural Science Bulletin, 2015, 31(2):159-165.
[13]赵锦,刘孟军,代丽,等.枣疯病病树中内源激素的变化研究[J].中国农业科学, 2006, 39(11):2255-2260.
ZHAO Jin, LIU Mehgiun, DAI Li, et al. The Variations of Endogenous Hormones in Chinese Jujube Infected with Witches' Broom Disease[J]. Scientia Agricultura Sinica, 2006, 39(11):2255-2260.
[14]HAN G Z. Evolution of jasmonate biosynthesis and signaling mechanisms [J]. Journal of Experimental Botany, 2016, 68(6):1323-1331.
[15]焦龙,蔡晓明,边磊,等.茉莉酸类化合物:从植物的诱导抗虫防御反应到生长-防御权衡[J].应用生态学报,2018,29(11):3876-3890.
JIAO Long, CAI Xiaoming, Bian Lei, et al. Jasmonates: From induced plant anti-herbivore defensive reaction to growth-defense tradeoffs [J]. Chinese Journal of Applied Ecology, 2018, 29(11): 3876-3890.
[16]骆琰妍,李魏,戴良英.不同激素信号途径在植物抗病中的相互作用研究进展[J]. 中国农学通报, 2013, 29(18):153-157.
LUO Yanyan, LI Wei DAI Liangying. The Progress of the Cross-talk Among the Signaling Pathways of Phytohormones in Plant Defense Responses [J].Chinese Agricultural Science Bulletin, 2013, 29(18):153-157
[17]巫建华,贾思振,冯英娜,等.茉莉酸合成关键酶基因FaOPR3调控草莓果实成熟[J].江苏农业学报,2016,32(5):1148-1154.
WU Jianhua, JIA Siahen, FENG Yingna, et al. Regulation of strawberry fruit ripening by jasmonic aicd synthes is gene FaOPR3[J]. Jiangsu of Agr.SCI , 2016, 32(5):1148-1154.
[18]高江曼,孟莹,刘庆,等.赤霞珠葡萄生长发育过程中内源激素的变化及其与果实成熟的关系[J].食品科学,2017,38(7):167-175.
GAO Jiangan, MENG Ying, LIU Qing, et al. Dynamic changes of endogenous hormones during the development of Vitis Vinifera L. cv. Cabernet Sauvignon [J]. Food Science, 2017, 38(7):167-175.