新疆农业科学 ›› 2024, Vol. 61 ›› Issue (3): 708-718.DOI: 10.6048/j.issn.1001-4330.2024.03.021
姚宇翔1,2(), 王国强2, 王可2, 伊莎2, 杨欣雅2, 罗晓霞1,2()
收稿日期:
2023-07-15
出版日期:
2024-03-20
发布日期:
2024-04-19
通信作者:
罗晓霞(1982-),女,新疆阿拉尔人,教授,博士,硕士生导师,研究方向为微生物资源及其利用,(E-mail)作者简介:
姚宇翔(1999-),女,河南周口人,硕士研究生,研究方向为微生物资源及其利用,(E-mail)1366307897@qq.com
基金资助:
YAO Yuxiang1,2(), WANG Guoqiang2, WANG Ke2, YI Sha2, YANG Xinya2, LUO Xioaxia1,2()
Received:
2023-07-15
Online:
2024-03-20
Published:
2024-04-19
Correspondence author:
LUO Xiaoxia(1982-),female,from Aral,Xinjiang,professor,research interests:microbial resources and their utilization,(E-mail) Supported by:
摘要:
【目的】 研究新疆塔里木河上游不同生境细菌群落结构及多样性。【方法】 采用免培养和可培养方法,研究塔里木河上游流域原始生境与人工开发后生境细菌群落结构及多样性差异,同时对分离的1株潜在新种进行多相分类鉴定。【结果】 原始生境与人工开发后生境细菌群落结构组成差异明显,人工开发后生境细菌α多样性低于原始生境,不同生境细菌β多样性差异较大。变形菌门和放线菌门为优势菌群,并获1株链霉菌属潜在新物种。【结论】 塔里木河上游不同生境样品中含有丰富的微生物资源,其细菌群落组成受人为活动的影响较大。
中图分类号:
姚宇翔, 王国强, 王可, 伊莎, 杨欣雅, 罗晓霞. 塔里木河上游不同生境细菌群落结构及多样性分析[J]. 新疆农业科学, 2024, 61(3): 708-718.
YAO Yuxiang, WANG Guoqiang, WANG Ke, YI Sha, YANG Xinya, LUO Xioaxia. Analysis of the bacterial communities structure and diversty of the Tarim River in different habitats[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 708-718.
Sample | Phylum | Class | Order | Family | Genus | Species |
---|---|---|---|---|---|---|
ZF | 19 | 43 | 99 | 143 | 226 | 88 |
EG | 26 | 54 | 129 | 160 | 236 | 82 |
TA | 21 | 54 | 103 | 142 | 178 | 47 |
BM | 22 | 44 | 105 | 156 | 221 | 70 |
S-T | 28 | 67 | 112 | 138 | 112 | 32 |
S-M | 25 | 60 | 89 | 101 | 78 | 13 |
S-Y | 26 | 55 | 97 | 120 | 95 | 36 |
S-m | 31 | 72 | 107 | 129 | 102 | 27 |
S4 | 26 | 62 | 116 | 143 | 117 | 30 |
S3 | 21 | 51 | 91 | 111 | 91 | 19 |
S2 | 28 | 65 | 110 | 136 | 114 | 33 |
S1 | 27 | 72 | 114 | 142 | 121 | 30 |
表1 各水平微生物分类单元数统计
Tab.1 Statistical table of the number of microbial classification units at all levels
Sample | Phylum | Class | Order | Family | Genus | Species |
---|---|---|---|---|---|---|
ZF | 19 | 43 | 99 | 143 | 226 | 88 |
EG | 26 | 54 | 129 | 160 | 236 | 82 |
TA | 21 | 54 | 103 | 142 | 178 | 47 |
BM | 22 | 44 | 105 | 156 | 221 | 70 |
S-T | 28 | 67 | 112 | 138 | 112 | 32 |
S-M | 25 | 60 | 89 | 101 | 78 | 13 |
S-Y | 26 | 55 | 97 | 120 | 95 | 36 |
S-m | 31 | 72 | 107 | 129 | 102 | 27 |
S4 | 26 | 62 | 116 | 143 | 117 | 30 |
S3 | 21 | 51 | 91 | 111 | 91 | 19 |
S2 | 28 | 65 | 110 | 136 | 114 | 33 |
S1 | 27 | 72 | 114 | 142 | 121 | 30 |
图1 不同样品间细菌群落结构组成 注:A:塔里木河上游不同土样门水平物种组成柱状图,B:塔里木河上游不同水样门水平物种组成柱状图,C:塔里木河上游不同土样属水平物种组成柱状图,D:塔里木河上游不同水样属水平物种组成柱状图
Fig.1 Analysis of bacterial community structure among different samples Note:A:Histogram of horizontal species composition of different soil phylums in the upper reaches of Tarim River,B:Histogram of horizontal species composition of different phylums of the upper reaches of Tarim River,C:Horizontal species composition of different soil genus in the upper reaches of Tarim River,D:Tarim Histogram of horizontal species composition of different water sample genera in the upper reaches of the river
Sample | Chao1 | Simpson | Shannon | Observed_species | Faith_pd | Goods_coverage |
---|---|---|---|---|---|---|
S1 | 3 377.93 | 0.990 888 | 9.297 34 | 3 249 | 306.966 | 0.994 019 |
S2 | 3 922.37 | 0.997 72 | 10.315 1 | 3 782 | 320.958 | 0.992 133 |
S3 | 2 191.48 | 0.980 822 | 8.072 1 | 2 042 | 206.968 | 0.994 716 |
S4 | 4 757.41 | 0.995 194 | 10.298 6 | 4 583 | 375.849 | 0.989 907 |
S-M | 3 705.76 | 0.968 635 | 8.987 97 | 3 508 | 281.87 | 0.991 298 |
S-m | 4 886.6 | 0.997 765 | 10.578 | 4 738 | 373.577 | 0.989 555 |
S-T | 5 273.96 | 0.996 183 | 10.872 6 | 5 260 | 400.71 | 0.996 595 |
S-Y | 4 452.27 | 0.998 124 | 10.712 5 | 4 230 | 364.662 | 0.990 527 |
BM | 3 900.91 | 0.884 219 | 7.023 68 | 3 830 | 180.009 | 0.995 676 |
EG | 3 070.09 | 0.965 024 | 7.240 96 | 3 063 | 178.746 | 0.998 786 |
TA | 912.77 | 0.978 809 | 7.514 6 | 910 | 72.96 | 0.999 898 |
ZF | 5 244.04 | 0.957 637 | 8.139 92 | 5 195 | 210.571 | 0.995 141 |
表2 不同样品的alpha多样性指数
Tab.2 Alpha diversity index of different samples
Sample | Chao1 | Simpson | Shannon | Observed_species | Faith_pd | Goods_coverage |
---|---|---|---|---|---|---|
S1 | 3 377.93 | 0.990 888 | 9.297 34 | 3 249 | 306.966 | 0.994 019 |
S2 | 3 922.37 | 0.997 72 | 10.315 1 | 3 782 | 320.958 | 0.992 133 |
S3 | 2 191.48 | 0.980 822 | 8.072 1 | 2 042 | 206.968 | 0.994 716 |
S4 | 4 757.41 | 0.995 194 | 10.298 6 | 4 583 | 375.849 | 0.989 907 |
S-M | 3 705.76 | 0.968 635 | 8.987 97 | 3 508 | 281.87 | 0.991 298 |
S-m | 4 886.6 | 0.997 765 | 10.578 | 4 738 | 373.577 | 0.989 555 |
S-T | 5 273.96 | 0.996 183 | 10.872 6 | 5 260 | 400.71 | 0.996 595 |
S-Y | 4 452.27 | 0.998 124 | 10.712 5 | 4 230 | 364.662 | 0.990 527 |
BM | 3 900.91 | 0.884 219 | 7.023 68 | 3 830 | 180.009 | 0.995 676 |
EG | 3 070.09 | 0.965 024 | 7.240 96 | 3 063 | 178.746 | 0.998 786 |
TA | 912.77 | 0.978 809 | 7.514 6 | 910 | 72.96 | 0.999 898 |
ZF | 5 244.04 | 0.957 637 | 8.139 92 | 5 195 | 210.571 | 0.995 141 |
图3 塔里木河上游PCoA分析 注:A:基于Bray curtis计算水样的PcoA分析; B:基于Bray Curtis计算土样的PCoA; C:基于非加权UniFrac距离的水样PCoA分析; D:基于非加权UniFrac距离的土样PCoA分析
Fig.3 PCoA analysis of the upper reaches of the Tarim River Note:A:Analysis of PCoA based on Bray-Curtis about Water sample; B:Analysis of PCoA based on Bray-Curtis about Soil sample; C:Analysis of PCoA based on unweighted UniFrac about Water sample; D:Analysis of PCoA based on unweighted UniFrac about soil sample
图8 菌株TRM49032的多相分类鉴定 注:A:菌株TRM 49032系统进化分析;B:菌株TRM 49032菌丝体形态;C:菌株TRM 49032细胞壁极性脂分析;D:菌株TRM 49032细胞壁脂肪酸分析;E:菌株TRM 49032细胞壁醌型分析
Fig.8 Multiphase classification and identification of strain TRM49032 Note:A:system evolution relationship of strain TRM49032; B:morphological electron micrograph of strain TRM49032; C:cell wall polar lipid type of strain TRM49032(ninhydrin staining,anisaldehyde staining,molybdenum phosphate staining); D:cell wall fatty acid type table of strain TRM49032; E:Analysis of cell wall quinotype of strain TRM49032
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