新疆农业科学 ›› 2022, Vol. 59 ›› Issue (9): 2123-2129.DOI: 10.6048/j.issn.1001-4330.2022.09.005

• 园艺特产·贮藏保鲜加工·土壤肥料·节水灌溉·草业·食品工艺技术 • 上一篇    下一篇

外源水杨酸对单盐胁迫下哈密瓜叶片解剖结构的影响

闫淼1(), 熊韬1, 黄全生2, 吴婷1, 吴海波1, 赵准2, 胡国智1()   

  1. 1.新疆农业科学院哈密瓜研究中心,乌鲁木齐 830091
    2.新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091
  • 收稿日期:2021-10-09 出版日期:2022-09-20 发布日期:2023-01-16
  • 通信作者: 胡国智
  • 作者简介:闫淼(1993-),女,河南洛阳人,助理研究员,硕士,研究方向为甜瓜抗逆栽培。(E-mail)yanmiao0901@126.com
  • 基金资助:
    新疆农作物生物技术重点实验室开放课题(XJYS0302-2022-3);海南省院士创新科研专项“黄皮网纹甜瓜选育及配套栽培技术集成与应用”(SQ2021PTZ0063);财政部和农业农村部:国家现代农业产业技术体系(CARS-25)

Effect of Exogenous Salicylic Acid(SA)on the Leaf Anatomical Structure of Hami Melon under NaCl Stress

YAN Miao1(), XIONG Tao1, HUANG Quansheng2, WU Ting1, WU Haibo1, ZHAO Zhun2, HU Guozhi1()   

  1. 1. Hami Melon Research Center,Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
    2. Research Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences,Urumqi 830091, China
  • Received:2021-10-09 Online:2022-09-20 Published:2023-01-16
  • Correspondence author: HU Guozhi
  • Supported by:
    Opening Project of Xinjiang Key Laboratory of Crop Biotechnology(XJYS0302-2022-3);Specific research fund of The Innovation Platform for Academinicians of Hainan Province(SQ2021PTZ0063);China Agriculture Research System of MOF and MARA(CARS-25)

摘要:

【目的】 研究外源水杨酸(SA)对单盐胁迫(NaCl)下哈密瓜叶片解剖结构的影响。【方法】 以哈密瓜新品种纳斯密为材料,采用种子发芽试验,筛选NaCl和SA最适浓度。运用石蜡切片技术,观察2个处理下哈密瓜幼苗叶片横切面,分析叶片厚度、上下表皮厚度及栅栏组织、海绵组织的变化,计算栅海比、叶片组织结构疏松度(SR)以及叶片组织结构紧密度(CTR),分析相关性。【结果】 NaCl胁迫下,哈密瓜叶片厚度、上下表皮厚度均出现了不断增加的趋势,栅栏组织排列疏松,叶肉间隙越来越大。而叶面喷施SA通过提高叶片上下表皮厚度、叶片厚度、栅栏组织厚度、栅海比,减小海绵组织厚度、SR,来缓解NaCl胁迫。2个处理下,栅栏组织厚度和CTR成极显著正相关,海绵组织厚度和SR成极显著正相关(P<0.01)。【结论】 100 mmol/LNaCl作为胁迫的浓度,0.25 mmol/LSA作为缓解浓度。SA处理可通过调节叶表皮和叶肉解剖结构的相关指标来缓解NaCl胁迫。

关键词: 哈密瓜; 水杨酸; 单盐胁迫; 叶片解剖结构

Abstract:

【Objective】 To investigate the effect of exogenous salicylic acid (SA) on the leaf anatomical structure of Hami-melon under single salt stress (NaCl). 【Methods】 In this experiment,the new Hami-melon variety Nasimi was used as the test material,and the optimum concentrations of NaCl and SA were screened by seed germination test. The changes of leaf thickness,upper and lower epidermal thickness,palisade and sponge thickness were analyzed,and the ratio of palisade thickness to sponge thickness,scattered rate (SR) and cell tightness rate(CTR) were calculated and correlation analysis was performed.【Results】 Under NaCl stress,the leaf thickness and upper and lower epidermal thickness of Hami-melon showed a trend of increasing,the palisade thickness were loosely arranged,and the leaf flesh gap was getting larger. After foliar spraying of SA,leaf upper and lower epidermal thickness,leaf thickness, palisade thickness,ratio of palisade thickness to sponge thickness increased,and spongy thickness and SR decreased. The palisade thickness and CTR were highly significantly positively correlated,and spongy thickness and SR were highly significantly positively correlated under the two treatments (P< 0.01). 【Conclusion】 100 mmol/L NaCl was used as the stress concentration and 0.25 mmol/L SA was used as the mitigation concentration. SA treatment could alleviate the stress by regulating the indexes related to leaf epidermis and leaf flesh anatomical structure

Key words: Hami melon; salicylic acid; NaCl stress; leaf anatomical structure

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

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