吐鲁番地区哈密瓜品种对细菌性果斑病抗性研究

doi:10.6048/j.issn.1001-4330.2017.11.012

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (11): 2054-2059.DOI: 10.6048/j.issn.1001-4330.2017.11.012

吐鲁番地区哈密瓜品种对细菌性果斑病抗性研究

毛亮¹, 吉艳玲¹, 张娟¹, 王宣仓¹, 潘卫萍¹, 张以和¹, 王晓东²

1.新疆吐鲁番市农业技术推广中心,新疆吐鲁番 838000;
2.石河子大学绿洲农作物病害防控重点实验室,新疆石河子 832000

收稿日期:2017-07-20 出版日期:2017-11-20 发布日期:2017-12-06
通信作者: 王晓东(1975-),男,山东莱州人,副教授,博士,研究方向为植物病害生物防治,(E-mail)wxdong11@163.com;张以和(1966-),男,江苏宿迁人,农业推广研究员,研究方向为设施栽培技术,(E-mail)1057065635@ qq.com
作者简介:毛亮(1981-),男,山东即墨人,高级农艺师,硕士,研究方向为设施病虫害,(E-mail)mao2548@163.com
基金资助:
国家自然科学基金项目“Pichia anomala 0732-1代谢产物乙酸在生防哈密瓜细菌性果斑病中的作用机制研究”(31460485);自治区青年科技创新人才培养工程“基层青年科技人才培养项目”(qn2015jc073)

A Preliminary Study on Resistance of Hami Melon Varieties to Acidovorax avenae subsp. citrulli in Turpan Area

MAO Liang¹, JI Yan-ling¹, ZHANG Juan¹, WANG Xuan-cang¹, PAN Wei-ping¹, ZHANG Yi-he¹, WANG Xiao-dong²

1.Xinjiang Turpan Agricultural Technology Extension Center, Turpan Xinjiang 838000, China;
2. Key Laboratory of Oasis Crop Disease Control and Prevention, Shihezi University, Shihezi Xinjiang 832000, China

Received:2017-07-20 Online:2017-11-20 Published:2017-12-06
Correspondence author: WANG Xiao-dong (1975- ), male, native place: Laizhou, Shangdong. Associate Professor,research field: Biological control of plant diseases. (E-mail)wxdong11@163.com;ZHANG Yi-he (1966- ), male, native place: Suqian, Jiangsu. Agricultural extension researcher, research field: Facility cultivation technology. (E-mail) 1057065635@qq.com
Supported by:
The National Natural Science Foundation of China "Study on Pichia anomala 0732-1 metabolic product acetic acid to the mechanism of preventing and controlling bacterial fruit blotch of Hami melon"(31460485) and Science and Autonomous region youth science and technology innovation talent training project "Grassroots young scientific and technological personnel training project" (qn2015jc073)

摘要/Abstract

摘要： 【目的】研究吐鲁番地区哈密瓜品种对细菌性果斑病的抗病性,为哈密瓜细菌性果斑病防治和抗病育种提供理论依据。【方法】以吐鲁番地区10份哈密瓜品种为材料,采用苗期人工接种进行病情指数调查;观察不同抗性品种苗期叶面绒毛数和气孔数及测定叶片自身防御酶PPO、POD。【结果】供试10个品种中无免疫品种,西州密25号和皇妃表现为中抗,新密36号表现为高感,其余7个品种表现为中感;高感品种新密36号和中感品种9818(绿皮)叶毛数无明显差距,西州密25号的叶毛数是新密36号的叶毛数1.68倍,气孔数是中感品种9818(绿皮)的1.42倍,是新密36号1.91倍;不同抗性品种PPO酶活性呈现感病品种﹥中感品种﹥抗病品种,POD酶活性呈现感病品种﹥中感品种﹥抗病品种。【结论】供试品种对哈密瓜细菌性果斑病抗病性有明显差异,未发现免疫和高抗品种,西州密25号和皇妃表现为中抗,新密36号为高感品种,其余品种表现为中感;品种抗病性与下表皮叶面叶毛数量和气孔数量有关,呈正相关趋势,自身防御酶PPO酶呈下降趋势,与品种抗病性呈负相关,POD酶呈上升趋势,与品种抗病性呈负相关。

关键词: 哈密瓜; 细菌性果斑病; 抗性

Abstract: 【Objective】 This project aims to provide the theoretical basis for the prevention and breeding of bacterial fruit blotch of Hami melon through the study on the resistance to Acidovorax avenae subsp. Citrulli in Turpan,【Method】10 Hami melon varieties planted in Turpan area were used as materials,and the disease index of resistance to Acidovorax avenae subsp. citrulli was investigated by artificial inoculation at the seedling stage. At the same time, the stomatal and leaf hair number of seedlings leaf surface between different resistant cultivars were compared and the defense enzymes PPO and POD in seedling leaves were measured.【Result】Among the 10 cultivars, there were no immune varieties, Xizhoumi No. 25 and Imperial Concubine showed the middle resistance, Xinmi No. 36 showed a high susceptibility, and the other seven varieties showed a middle susceptibility; There were no significant differences in leaf hair number between susceptible varieties Xinmi No. 36 and middle susceptible varieties 9818 (green leaf trichomes). Leaf hair number of the middle resistance Xizhoumi No.25 was 1.68 times that of Xinmi No. 36. The number of stomata of Xizhoumi No. 25 was 1.42 times that of 9818 (green leaf trichomes) and 1.91 times that of Xinmi No.36. The PPO enzyme activities of different resistant varieties showed susceptible varieties > middle susceptible varieties > resistant varieties, POD enzyme activity showed susceptible varieties > middle susceptible varieties > resistant varieties.【Conclusion】Different Hami melon varieties have significant difference resistance to Acidovorax avenae subsp. Citrulli, and no immune and high resistant varieties among them have been found. Xizhoumi No. 25 and Imperial Concubine show middle resistance, Xinmi No. 36 is a high susceptible variety, other varieties are middle and high susceptible varieties. There was a positive correlation between disease resistance and the number of leaf hair and stomata, showing positive correlation trend. The PPO enzyme showed a downward trend, which was negatively related to disease resistance, and POD enzyme showed an upward trend, which was negatively related to disease resistance of varieties.

Key words: Hami melon; bacterial fruit blotch; resistance

中图分类号:

毛亮, 吉艳玲, 张娟, 王宣仓, 潘卫萍, 张以和, 王晓东. 吐鲁番地区哈密瓜品种对细菌性果斑病抗性研究[J]. 新疆农业科学, 2017, 54(11): 2054-2059.

MAO Liang, JI Yan-ling, ZHANG Juan, WANG Xuan-cang, PAN Wei-ping, ZHANG Yi-he, WANG Xiao-dong. A Preliminary Study on Resistance of Hami Melon Varieties to Acidovorax avenae subsp. citrulli in Turpan Area[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2054-2059.

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吐鲁番地区哈密瓜品种对细菌性果斑病抗性研究

A Preliminary Study on Resistance of Hami Melon Varieties to Acidovorax avenae subsp. citrulli in Turpan Area

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