调节剂对低温胁迫后恢复过程中棉花幼苗生长与生理特性的影响

doi:10.6048/j.issn.1001-4330.2022.05.003

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (5): 1060-1067.DOI: 10.6048/j.issn.1001-4330.2022.05.003

• 作物遗传育种·耕作栽培·生理生化·种质资源 • 上一篇下一篇

调节剂对低温胁迫后恢复过程中棉花幼苗生长与生理特性的影响

周小云¹(), 阿达来提·阿布都热依木², 王立红¹, 李进¹, 张军高¹^,³, 刘梦丽⁴, 雷斌¹(), 薄瑞²()

1.新疆农业科学院核技术生物技术研究所/农业农村部荒漠绿洲作物生理生态与耕作重点实验室/新疆作物化学调控工程技术研究中心,乌鲁木齐 830091
2.新疆维吾尔自治区农药检定所,乌鲁木齐 830049
3.新疆绿洲兴源农业科技有限责任公司,乌鲁木齐 830091
4.新疆维吾尔自治区植保站,乌鲁木齐 830006

收稿日期:2021-09-20 出版日期:2022-05-20 发布日期:2022-06-09
通信作者: 雷斌,薄瑞
作者简介:周小云(1977-),男,重庆人,研究员,博士,研究方向为作物化控与生物技术,(E-mail) xiaoyunzhou77@126.com
基金资助:
自治区天山雪松计划“小麦、棉花提质增效关键技术及产品研发”(2020XS16);自治区重点实验室开放课题“萎锈灵提高棉苗耐低温冷害能力的分子作用机制”(2020D04017);国家自然科学基金“萎锈灵提高棉苗抗冷性的分子机制”(32060429)

Effects of Regulators on the Growth and Physiological Characteristics in Cotton Seedling during Restoration after Chilling Stress

ZHOU Xiaoyun¹(), Adalaiti Abduriyimu², WANG Lihong¹, LI Jin¹, ZHANG Jungao¹^,³, LIU Mengli⁴, LEI Bin¹(), BO Rui²()

1. Research Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences / Key Laboratory of Oasis-Desert Crop Physiology Ecology and Cultivation of Ministry of Agricultural and Rural Affairs / Xinjiang Research Center of Engineering and Technology for Crop Chemical Regulation,Urumqi 830091,China
2. Institute for the Control of Agrochemicals of Xinjiang Uygur Autonomous Region, Urumqi 830049, China
3. Xinjiang Oasis Xingyuan Agricultural Science and Technology Co., Ltd, Urumqi 830091, China
4. Plant Protection Station of Xinjiang Uygur Autonomous Region, Urumqi 830006, China

Received:2021-09-20 Online:2022-05-20 Published:2022-06-09
Correspondence author: LEI Bin, BO Rui
Supported by:
Tianshan Cedar Program project "Key Technologies for Improving Quality and Efficiency of Wheat and Cotton and Product Research and Development"(2020XS16);Opening Project of Key Laboratory in Xinjiang Uygur Autonomous Region "The Molecular Mechanism of Carboxin Improving the Cold Resistance of Cotton Seedling"(2020D04017);the National Foundation of China "Mechanism of Carboxin on Improving Chilling Resistance of Cotton Seedling "(32060429)

摘要/Abstract

摘要：

【目的】棉苗经低温胁迫后喷施调节剂,研究不同植物生长调节剂对棉花幼苗低温冷害的修复作用,为棉花幼苗抵御低温冷害提供技术参考。【方法】以棉花品种新陆早57号为材料,采用人工模拟低温方法,在子叶期4℃ 24 h处理后,叶面喷施清水为对照,分别喷施7种不同植物调节剂溶液后,置于25℃ 14 h/10 h(昼/夜)的人工气候箱内恢复,分析不同生长调节剂对棉花幼苗冷害的生长情况和生理生化特征的影响。【结果】与清水处理比较,喷施植物生长调节剂后棉花幼苗的株高增加了5.10%~9.87%,茎粗增加了5.28%~8.71%,主根长增加了5.24%~9.33%,鲜重增加了2.26%~5.98%,干重增加了2.38%~8.61%,冷害指数下降了1.79~5.29个百分点,脯氨酸增加了5.04%~5.58%,可溶性蛋白含量增加了5.07%~5.67%,可溶性糖含量增加了5.05%~5.87%;CAT酶活性增加了2.21%~7.95%。SOD酶活性增加了3.79%~5.56%,POD酶活性增加了2.18%~5.56%。【结论】在低温胁迫后,在常温恢复过程中叶面喷施生长调节剂可有效的提高棉花幼苗叶片的渗透调节能力和抗氧化能力,促进植株生长,修复低温胁迫对植株的伤害,以植物生长调节剂处理提高棉花幼苗抗寒性。其中,调节剂对低温胁迫后棉花幼苗恢复效果的综合作用排序为“阿泰灵”>α-萘乙酸钠>胺鲜脂>“碧护”>复硝酚钠>腐殖酸>黄腐酸钾Ⅱ型>对照。

关键词: 棉花; 低温胁迫; 调节剂; 生长; 生理特性

Abstract:

【Objective】 After chilling stress, cotton seedlings were sprayed with plant growth regulators to explore the repair effects of different plant growth regulators on chilling stress of cotton seedlings, so as to provide technical reference for cotton seedlings to resist chilling. 【Methods】 The cotton variety 'Xinluzao 57' was treated at 4℃ for 24 hours at the cotyledon stage by artificial simulated chilling environment. The leaves were sprayed with clear water as the control, and 7 different plant regulator solutions were sprayed respectively. The effects of growth regulators on the growth and physiological and biochemical characteristics of cotton seedlings during restoration after chilling Stress were analyzed. 【Results】 The results showed that compared with the control, after spraying growth regulator, the plant height of cotton seedlings increased by 5.10% - 9.87%, stem diameter increased by 5.28% - 8.71%, root length increased by 5.24% - 9.33%, fresh weight increased by 2.26% - 5.98%, dry weight increased by 2.38% - 8.61%, chilling injury index decreased by 1.79% - 5.29%, the content of proline increased by 5.04% - 5.58%, the content of soluble protein increased by 5.07% - 5.67%, the content of soluble sugar increased by 5.05% - 5.87%, SOD activity increased by 3.79%-5.56%, POD enzyme activity increased by 2.18%-5.56%, CAT enzyme activity increased by 2.21% - 7.95%.【Conclusion】 After chilling stress, spraying growth regulators on the leaves during the recovery of normal temperature can effectively improve the osmotic adjustment ability and antioxidant ability of cotton seedling leaves, promote plant growth and repair the damage to the plant caused by chilling stress. This is a convenient and practical agronomic measure to improve the resistance of cotton to chilling. For the cotton area in Xinjiang where chilling damage occurs frequently in early spring, the technology of using plant growth regulator to improve the chilling resistance of cotton seedlings has a certain application prospect. Among them, the comprehensive effects of regulators on the restoration of cotton seedlings after chilling stress are as follows: "ATAILIN" > α- Sodium naphthylacetate >Diethyl aminoethyl hexanoate> "BIHU" > Compound Sodium Nitrophenolate > Humic acid >Potassium fulvic acid type II >Control.

Key words: cotton; chilling stress; regulator; growth; physiological characteristics

中图分类号:

S562

周小云, 阿达来提·阿布都热依木, 王立红, 李进, 张军高, 刘梦丽, 雷斌, 薄瑞. 调节剂对低温胁迫后恢复过程中棉花幼苗生长与生理特性的影响[J]. 新疆农业科学, 2022, 59(5): 1060-1067.

ZHOU Xiaoyun, Adalaiti Abduriyimu, WANG Lihong, LI Jin, ZHANG Jungao, LIU Mengli, LEI Bin, BO Rui. Effects of Regulators on the Growth and Physiological Characteristics in Cotton Seedling during Restoration after Chilling Stress[J]. Xinjiang Agricultural Sciences, 2022, 59(5): 1060-1067.

图/表 5

图1 低温处理后恢复过程中生长调节剂处理棉花幼苗生长特点和冷害指数的比较注:短线表示标准差,小写字母表示各处理在P≤0.05水平上的差异显著性。CK指清水处理,T1~T7分别“碧护”,“阿泰灵”,胺鲜脂,复硝酚钠,α-萘乙酸钠,黄腐酸钾Ⅱ型,腐殖酸处理,下同

Fig.1 Comparison of growth characteristics and chilling injury index in cotton seedling during restoration after chilling stress Note:The bar is mean standard deviation.Lowercase letters indicate the significant difference among treatments at 0.05 level.CK refers to distilled water treatment, T1-T7 are "Bihu", "atailin", amine fresh fat, sodium dinitrophenol, sodium α-naphthalene acetate, potassium fulvic acid type II and humic acid treatment respectively.The same as below

图2 低温胁迫后恢复过程中调节剂处理棉花幼苗细胞膜系统指标的比较

Fig.2 Comparison of membrane systems indexes in cotton seedling during restoration after chilling stress

图3 低温胁迫后恢复过程中调节剂处理棉花幼苗渗透调节物质指标的比较

Fig.3 Comparison of osmotic regulation substances indexes in cotton seedling during restoration after chilling stress

图4 低温胁迫后恢复过程中调节剂处理棉花幼苗抗氧化酶活性指标的比较

Fig.4 Comparison of antioxidant enzyme activities indexes in cotton seedling during restoration after chilling stress

表2 低温胁迫后恢复过程中调节剂处理棉花幼苗耐低温冷害能力的比较

Table 2 Comparison of cold tolerance of cotton seedling during restoration after chilling stress

处理 Treatments	F₁	F₂	F₃	F	排序 Sort
CK	-3.04	3.54	-2.36	-2.74	8
T₁	2.84	2.33	-2.96	4.25	1
T₂	3.95	2.39	-6.57	1.78	2
T₃	-1.67	-1.75	5.07	0.96	3
T₄	-1.51	-1.75	3.37	-0.82	5
T₅	-4.42	3.04	2.93	0.70	4
T₆	-2.02	3.01	-2.11	-1.55	7
T₇	1.21	-1.27	-1.72	-1.91	6

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调节剂对低温胁迫后恢复过程中棉花幼苗生长与生理特性的影响

Effects of Regulators on the Growth and Physiological Characteristics in Cotton Seedling during Restoration after Chilling Stress

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