陆地棉COR基因在低温胁迫下的表达分析及功能鉴定

doi:10.6048/j.issn.1001-4330.2020.03.002

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (3): 401-407.DOI: 10.6048/j.issn.1001-4330.2020.03.002

• 作物科学·分子生物·园艺·林业 • 上一篇下一篇

陆地棉COR基因在低温胁迫下的表达分析及功能鉴定

李建平, 足木热木·吐尔逊, 郝晓燕, 常晓春, 高升旗, 胡文冉, 陈果, 黄全生

新疆农业科学院核技术生物技术研究所/新疆农作物生物技术重点实验室,乌鲁木齐 830091

收稿日期:2019-11-07 出版日期:2020-03-20 发布日期:2020-03-11
通信作者: 黄全生(1964-),男,新疆乌鲁木齐人,研究员,博士,研究方向为作物耐逆基因资源挖掘及分子基础, (E-mail)hquansheng@126.com
作者简介:李建平(1977-),男,新疆乌鲁木齐人,副研究员,博士,研究方向为植物抗逆基因,(E-mail) ljp7786@126.com
基金资助:
新疆维吾尔自治区重点实验室开放课题“棉花耐冷的分子遗传基础及关键基因功能研究”(2015KL008)

Expression Analysis and Functional Identification of COR Gene in Gossypium hirsutum L under Low Temperature Stress

LI Jianping, Zumuremu Tuerxun, HAO Xiaoyan, CHANG Xiaochun, GAO Shengqi, HU Wenran, CHEN Guo, HUANG Quansheng

Key Laboratory of Xinjiang Crop Biotechnology / Research Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2019-11-07 Online:2020-03-20 Published:2020-03-11
Correspondence author: Quansheng Huang(1964-), male, Urumqi, PhD, Researcher, Specialize in gene identification and molecular mechanism for abiotic stress resistance in crops,(E-mail)hquansheng@126.com
Supported by:
Supported by Key Open Project of Xinjang Uygur Autonomous Region “Study on Molecular Genetic Basis and Key Gene Function of Cold Tolerance in Cotton (Gossypium hirsutum L.) (2015KL008)

摘要/Abstract

摘要： 【目的】分析低温胁迫条件下陆地棉中COR家族成员的表达特性,研究参与低温胁迫信号途径的COR基因。【方法】通过Real-time PCR分析低温胁迫条件下,陆地棉COR基因家族成员的表达情况,利用VIGS技术鉴定响应低温胁迫的COR基因功能。【结果】对8个COR基因家族成员在低温条件下的表达分析显示,基因序列号为Gh_D12G0215的COR基因受低温诱导表达水平不断上调,并在处理48 h后表达量较对照上调了近9倍;与之相反,基因序列号为Gh_D06G0147的COR基因在低温诱导3 h左右表达水平出现短暂的上调,但随后其表达受到显著抑制。其余COR基因家族成员的表达受低温胁迫表达水平发生改变,但是随着胁迫时间的增加,转录水平与对照无明显差异。利用VIGS技术在棉花中沉默候选COR基因Gh_D12G0215,并将基因沉默后的棉苗与对照共同进行低温胁迫处理,在相同的处理条件下,Gh_D12G0215基因沉默后的植株与对照相比,对低温胁迫更为敏感,基因序列号为Gh_D12G0215的COR基因可能参与棉花耐低温的调控。【结论】在低温胁迫条件下,陆地棉中部分COR基因的表达受低温胁迫诱导,在陆地棉受到低温胁迫时发挥功能。

关键词: 陆地棉; 低温胁迫; COR基因; VIGS

Abstract: 【Objective】 To identify COR genes that mediated the tolerance to low temperature in cotton by analyzing expressing pattern of COR gene family members and VIGS gene silencing technology. 【Method】The real-time PCR was performed to analyze the transcriptional level of CORs in response to low temperature stress in cotton, as well as phenotype of the COR gene silenced cotton seedlings by virus induced gene silencing system (VIGS). 【Result】 The real-time PCR results showed that the mRNA accumulation of a COR gene (GenBank ID Gh_D12G0215) was induced by low temperatures and increased with the extended time of low temperature treatment, which the mRNA accumulation level was 9 folds of control plants at 48 h time point. On the contrary, the expression of another COR gene member (GenBank ID Gh_D06G0147) was suppressed in response to low temperature although there was an initially up-regulated expression at 3h time-point after low temperature treatment. For the rest COR family members, their mRNA accumulation showed no significant difference compared with the control. Meanwhile, the candidate COR gene member Gh_D12G0215 was silenced and treated with low temperature to confirm its function in the regulation of low temperature. By comparing growing condition with the non-silenced plants, the Gh_D12G0215 silenced plants showed less tolerance to low temperature, indicating that the Gh_D12G0215 may play a role in resistance to low temperature. 【Conclusion】 The mRNA accumulation of some COR genes were induced by low temperature stress, and one of them may play a role in regulation of low temperature stress pathway.

Key words: Gossypium hirsutum L; low temperature stress; COR gene; VIGS

中图分类号:

S562

李建平, 足木热木·吐尔逊, 郝晓燕, 常晓春, 高升旗, 胡文冉, 陈果, 黄全生. 陆地棉COR基因在低温胁迫下的表达分析及功能鉴定[J]. 新疆农业科学, 2020, 57(3): 401-407.

LI Jianping, Zumuremu Tuerxun, HAO Xiaoyan, CHANG Xiaochun, GAO Shengqi, HU Wenran, CHEN Guo, HUANG Quansheng. Expression Analysis and Functional Identification of COR Gene in Gossypium hirsutum L under Low Temperature Stress[J]. Xinjiang Agricultural Sciences, 2020, 57(3): 401-407.

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陆地棉COR基因在低温胁迫下的表达分析及功能鉴定

Expression Analysis and Functional Identification of COR Gene in Gossypium hirsutum L under Low Temperature Stress

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