达坂城蚕豆根腐病病原菌鉴定及生防菌拮抗效果分析

doi:10.6048/j.issn.1001-4330.2019.010.016

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (10): 1904-1911.DOI: 10.6048/j.issn.1001-4330.2019.010.016

• 土壤肥料·微生物·植物保护·农产品加工·农业经济 • 上一篇下一篇

达坂城蚕豆根腐病病原菌鉴定及生防菌拮抗效果分析

楚敏, 顾美英, 唐琦勇, 朱静, 郝秀英, 张志东

新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091

收稿日期:2019-03-12 发布日期:2019-11-22
通信作者: 张志东(1977-),男,新疆人,研究员,研究方向为特殊环境微生物学,(E-mail)zhangzheedong@sohu.com
作者简介:楚敏(1977-),女,新疆人,副研究员,硕士,研究方向为微生物学和分子生物学,(E-mail)chuliu2002@163.com
基金资助:
国家自然科学基金(31460148);自治区科技创新基地建设(资源共享平台建设)专项“新疆特殊环境微生物菌种资源共享平台建设”(PT1805)

Identification of Pathogenic Fungi Causing the Root Rot Disease of Dabancheng Faba Bean and Analysis of Antagonistic Effect by the Biocontrol Bacteria

CHU Min, GU Mei-Ying, TANG Qi-yong, ZHU Jing, HAO Xiu-ying, ZHANG Zhi-dong

Xinjiang Laboratory of Special Environmental Microbiology/Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2019-03-12 Published:2019-11-22
Correspondence author: ZHANG Zhi-dong (1977-), male, native place: Urumqi, Xinjiang, Professor,research field: Microbial ecology , (E-mail)zhangzheddong@sohu.com

摘要/Abstract

摘要： 【目的】研究鉴定达坂城蚕豆根腐病病原菌,分析生防菌对病原菌的拮抗效果,为高效生物防治提供了科学依据和理论基础。【方法】采集达坂城蚕豆根腐病病株,进行病原菌分离及微生物分子鉴定,分析生防菌拮抗效果。【结果】达坂城蚕豆根腐病病原菌为厚孢镰孢菌变种(Fusarium chlamydosporum var. fuscum),其对达坂城蚕豆具有很强的侵染性,可达到100%致病。所采用的生防菌B6和B1均对病原菌株具有较好的抑制效果,其最大抑菌圈菌落比可达到1.90。【结论】达坂城蚕豆根腐病病原菌为厚孢镰孢菌变种(Fusarium chlamydosporum var. fuscum)。

关键词: 蚕豆; 根腐病; 病原菌; 生防菌

Abstract: 【Objective】 To identify pathogens causing root rot disease in Dabancheng faba bean and analyze antagonistic effects by the biocontrol bacteria.【Method】 Diseased plants of Dabancheng faba bean were collected, and pathogenic fungi were isolated and identified by the molecular method. And also, the inhibitory effects of biocontrol bacteria on pathogenic fungi were tested.【Result】 The results showed the root rot disease of Dabancheng faba bean was caused by Fusarium chlamydosporum var. fuscum. Pathogenicity analysis displayed that it had strong infectivity to Dabancheng faba bean and pathogenicity could reach 100%. Both of biocontrol bacteria B6 and B1 had good inhibitory effect on the pathogen fungus, and the rate of inhibition zone to colony could reach 1.90.【Conclusion】 Fusarium chlamydosporum var. fuscum causing the root rot disease of Dabanchen faba bean was reported firstly, which, meanwhile, provided the scientific basis and theory for efficient biological control of faba bean root rot.

Key words: faba bean; root rot disease; pathogen; biocontrol bacteria

中图分类号:

:S435.23

楚敏, 顾美英, 唐琦勇, 朱静, 郝秀英, 张志东. 达坂城蚕豆根腐病病原菌鉴定及生防菌拮抗效果分析[J]. 新疆农业科学, 2019, 56(10): 1904-1911.

CHU Min, GU Mei-Ying, TANG Qi-yong, ZHU Jing, HAO Xiu-ying, ZHANG Zhi-dong. Identification of Pathogenic Fungi Causing the Root Rot Disease of Dabancheng Faba Bean and Analysis of Antagonistic Effect by the Biocontrol Bacteria[J]. Xinjiang Agricultural Sciences, 2019, 56(10): 1904-1911.

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达坂城蚕豆根腐病病原菌鉴定及生防菌拮抗效果分析

Identification of Pathogenic Fungi Causing the Root Rot Disease of Dabancheng Faba Bean and Analysis of Antagonistic Effect by the Biocontrol Bacteria

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