新疆农业科学 ›› 2019, Vol. 56 ›› Issue (3): 498-508.DOI: 10.6048/j.issn.1001-4330.2019.03.013

• 棉花化肥农药减施研究 • 上一篇    下一篇

生防菌HFW217的鉴定及其对棉花枯萎病的防治效果

丁建朋, 范瑛阁, 姚永生   

  1. 塔里木大学植物科学学院,新疆阿拉尔,843300
  • 收稿日期:2018-12-12 出版日期:2019-03-20 发布日期:2019-06-25
  • 通信作者: 范瑛阁(1978-),女,河南省南阳人,副教授,硕士,研究方向为植物病害生物防治,(E-mail) fygzky@163.com;姚永生(1977-),男,河南省宁陵人,副教授,博士,研究方向为有害生物综合治理,(E-mail) yyszky@163.com
  • 作者简介:丁建朋(1995-),男,河南省镇平人,硕士研究生,研究方向为有害生物综合治理,(E-mail) 3377198766@qq.com
  • 基金资助:
    国家重点研发计划“棉花化肥农药减施增效技术集成与示范”(2017YFD0201900);新疆干旱区盐碱地生态治理关键技术研究与集成示范" (2016FYC0501400)

Identification and Control Effect on Cotton Fusarium wilt of Biocontrol Bacteria HFW217

DING Jian-peng, FAN Ying-ge, YAO Yong-sheng   

  1. College of Plant Science, Tarim University, Aral Xinjiang 843300 China
  • Received:2018-12-12 Online:2019-03-20 Published:2019-06-25
  • Correspondence author: FAN Ying-ge (1978-), female, associate professor, mainly engaged in biological control of plant diseases research, (E-mail) fygzky@163.com;
    YAO Yong-sheng (1977-), male, associate professor, doctor, research field: Integrated pest management, (E-mail) yyszky@163.com
  • Supported by:
    Supported by: the National Key R & D Program of China "Technical Integration and Demonstration of Reducing Fertilizer and Pesticide Application and Increasing the Efficiency on Cotton" (2017YFD0201900) and "Key Technologies Research and Integrated Demonstration of Saline-alkali Field Ecological Management in Xinjiang Arid Region" (2016FYC0501400);

摘要: 目的 研究生防菌HFW217的种类及其对棉花枯萎病的防治效果,为其生物防治提供材料。方法 采用16 SrDNA、rpoB基因序列和生理生化方法,对HFW217进行鉴定,通过温室和田间药效试验,测定HFW217对棉花枯萎病的防效。结果 菌株HFW217为革兰氏阳性菌、呈短杆状、有芽孢,其菌体的形态呈短杆状,具有一定的耐盐碱性,16S rDNA和rpoB基因序列与解淀粉芽孢杆菌Bacillus amloliquefaciens具有较高的同源性,经鉴定该菌是解淀粉芽孢杆菌B. amloliquefaciens。温室和田间对棉花枯萎病防治效果分别达到77.62%和73.18%,防效明显高于多菌灵。结论 菌株HFW217是解淀粉芽孢杆菌,该菌对棉花枯萎病的预防和治疗作用显著。

关键词: 棉花枯萎病; 解淀粉芽孢杆菌; 生防菌; 鉴定; 防治效果

Abstract: Objective To clarify the types of biocontrol bacteria HFW217 and its control effects against cotton Fusarium wilt. Method The 16S rDNA,rpoB gene sequence and physiological and biochemical method were adopted to identify HFW217, and the biocontrol effects of HFW217 were determined through greenhouse and field efficacy trials.Result The analysis of salt tolerance showed that strain HFW217 had certain salt tolerance. The strain HFW217 microscopy was Gram-positive bacteria; It was short rod-shaped and spore-like, and its form wais short rod-like. 16S rDNA and rpoB gene sequence had high homology with that of Bacillus amloliquefaciens homology. Identification results showed that biocontrol bacteria HFW217 was Bacillus amloliquefaciens; its preventive and control effects against cotton Fusarium wilt in the greenhouse reached 77.62% and 73.18%, respectively; Its control effects were significantly higher than that of carbendazim. Conclusion The results showed that strain HFW217 was Bacillus amloliquefaciens , which had potential application in the biological control of Fusarium wilt of cotton.

Key words: Fusarium wilt of cotton; Bacillus amyloliquefaciens; biocontrol bacteria; identification; control effects

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

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