新疆农业科学 ›› 2023, Vol. 60 ›› Issue (12): 3094-3101.DOI: 10.6048/j.issn.1001-4330.2023.12.027
• 畜牧兽医·微生物·农业装备工程与机械化 • 上一篇 下一篇
收稿日期:
2023-04-09
出版日期:
2023-12-20
发布日期:
2024-01-03
通信作者:
陆 婷(1970-),女,江苏人,副教授,博士,研究方向为园艺植物繁殖生物学,(E-mail)luting0909@126.com
作者简介:
徐珊珊(1998-),女,山东人,硕士研究生,研究方向为园林植物应用,(E-mail)853946533@qq.com
基金资助:
XU Shanshan1(), WANG Huanyu1, LU Ting1(), LIU Aimin2
Received:
2023-04-09
Online:
2023-12-20
Published:
2024-01-03
Correspondence author:
LU Ting(1970-),female,ancestral home is Jiangsu,associate professor,Doctor,research direction is horticultural plant reproduction biology,(E-mail)Supported by:
摘要:
【目的】研究下延叶古当归(Archangelica decurrens Ledeb.)种子休眠特性并找到解除休眠的方法,为该物种种质资源保护和开发利用提供科学依据。【方法】以下延叶古当归野生种子为材料,通过室内控制实验法对果实和种子基本生物学特性、种子萌发抑制物质进行研究,分析低温层积处理以及赤霉素不同浓度和时间交互处理对其萌发率、萌发势、萌发指数的影响。【结果】种皮不阻碍种子吸水;种子粉末浸取液对白菜(Brassica pekinensis)种子萌发及幼苗生长具有显著抑制作用;30~50 d低温层积处理能极显著提高下延叶古当归种子萌发率;果实形态成熟时种胚发育不完全,低温层积能促进种胚发育;使用3 000mg/L赤霉素浸种24 h可使种子萌发率显著提升至72.00%。【结论】下延叶古当归种子休眠是由种子内萌发抑制物和种胚发育不完全共同作用引起的形态生理休眠,播种前将种子低温层积30~50 d,或使用2 000~4 000 mg/L浓度的赤霉素浸种24 h可有效解除种子休眠从而促进种子萌发。
中图分类号:
徐珊珊, 王环宇, 陆婷, 刘爱民. 下延叶古当归种子休眠特性与解除方法[J]. 新疆农业科学, 2023, 60(12): 3094-3101.
XU Shanshan, WANG Huanyu, LU Ting, LIU Aimin. Dormancy characteristics and breaking methods of Archangelica decurrens seeds[J]. Xinjiang Agricultural Sciences, 2023, 60(12): 3094-3101.
种子萌发指标 Seed germination indicator | 对照组 Control group | 处理组 Treatment group | 幼苗生长指标 Seedling growth indicator | 对照组 Control group | 处理组 Treatment group |
---|---|---|---|---|---|
萌发率 Germination rate(%) | 98.0±1.63Aa | 10.7±6.18Bb | 下胚轴长 Hypocotyl length(mm) | 9.96±1.54Aa | 9.74±0.70Aa |
萌发势 Germination potential(%) | 44.0±2.83Aa | 2.0±1.63Bb | 鲜重 Fresh weight(g) | 1.40±0.11Aa | 0.87±0.14Ab |
萌发指数 Germination index | 38.0±1.47Aa | 1.5±1.22Bb | 根长 Root length(mm) | 14.67±1.01Aa | 9.59±0.32Bb |
表1 下延叶古当归种子粉末浸取液下白菜种子萌发及幼苗生长变化
Tab.1 Effects of seed powder leaching liquid of A.decurrens on seed germination and seedling growth of B.pekinensis
种子萌发指标 Seed germination indicator | 对照组 Control group | 处理组 Treatment group | 幼苗生长指标 Seedling growth indicator | 对照组 Control group | 处理组 Treatment group |
---|---|---|---|---|---|
萌发率 Germination rate(%) | 98.0±1.63Aa | 10.7±6.18Bb | 下胚轴长 Hypocotyl length(mm) | 9.96±1.54Aa | 9.74±0.70Aa |
萌发势 Germination potential(%) | 44.0±2.83Aa | 2.0±1.63Bb | 鲜重 Fresh weight(g) | 1.40±0.11Aa | 0.87±0.14Ab |
萌发指数 Germination index | 38.0±1.47Aa | 1.5±1.22Bb | 根长 Root length(mm) | 14.67±1.01Aa | 9.59±0.32Bb |
层积天数 Stratification days(d) | 萌发率 Germination rate(%) | 差异显著性 Significance of difference | 萌发势 Germination potential(%) | 差异显著性 Significance of difference | 萌发指数 Germination index | 差异显著性 Significance of difference | |||
---|---|---|---|---|---|---|---|---|---|
0.05 | 0.01 | 0.05 | 0.01 | 0.05 | 0.01 | ||||
0 | 6.67 | c | C | 3.33 | c | C | 0.47 | e | C |
10 | 12.00 | bc | BC | 8.00 | c | BC | 0.63 | e | C |
20 | 20.00 | b | B | 7.33 | c | BC | 1.24 | d | C |
30 | 60.00 | a | A | 17.33 | b | B | 3.55 | c | B |
40 | 61.33 | a | A | 29.33 | a | A | 4.86 | b | A |
50 | 66.67 | a | A | 35.33 | a | A | 5.62 | a | A |
表2 低温层积处理中下延叶古当归种子萌发率、萌发势、萌发指数变化
Tab.2 Effects of cold stratification treatment on seed germination rate, germination potential and germination index of A.decurrens
层积天数 Stratification days(d) | 萌发率 Germination rate(%) | 差异显著性 Significance of difference | 萌发势 Germination potential(%) | 差异显著性 Significance of difference | 萌发指数 Germination index | 差异显著性 Significance of difference | |||
---|---|---|---|---|---|---|---|---|---|
0.05 | 0.01 | 0.05 | 0.01 | 0.05 | 0.01 | ||||
0 | 6.67 | c | C | 3.33 | c | C | 0.47 | e | C |
10 | 12.00 | bc | BC | 8.00 | c | BC | 0.63 | e | C |
20 | 20.00 | b | B | 7.33 | c | BC | 1.24 | d | C |
30 | 60.00 | a | A | 17.33 | b | B | 3.55 | c | B |
40 | 61.33 | a | A | 29.33 | a | A | 4.86 | b | A |
50 | 66.67 | a | A | 35.33 | a | A | 5.62 | a | A |
图2 下延叶古当归种胚发育纵剖 注:A:新鲜种子;B:低温层积50 d的种子;C:胚根突破种皮的种子
Fig.2 Longitudinal view of embryo development of A.decurrens Note:A:the fresh seed; B:the seed after cold stratification for 50 d; C:the seed radicle breaks through seed coat
层积天数 Stratification days(d) | 胚率 Embryo rate(%) | 胚率差异显著性 Embryo rate significance of difference | |
---|---|---|---|
0.05 | 0.01 | ||
0 | 15.70 | d | C |
10 | 18.03 | cd | BC |
20 | 19.13 | c | B |
30 | 20.23 | c | B |
40 | 24.69 | b | A |
50 | 27.67 | a | A |
表3 低温层积过程中下延叶古当归种子胚率变化
Tab.3 Changes of seed embryo rate of A.decurrens in the process of cold stratification
层积天数 Stratification days(d) | 胚率 Embryo rate(%) | 胚率差异显著性 Embryo rate significance of difference | |
---|---|---|---|
0.05 | 0.01 | ||
0 | 15.70 | d | C |
10 | 18.03 | cd | BC |
20 | 19.13 | c | B |
30 | 20.23 | c | B |
40 | 24.69 | b | A |
50 | 27.67 | a | A |
赤霉素浓度 Gibberellin concentration (mg/L) | 浸种时间 Seed soaking time (h) | 萌发率 Germin- ation rate (%) | 差异显著性 Significance of difference | 萌发势 Germination Potential (%) | 差异显著性 Significance of difference | 萌发指数 Germination index | 差异显著性 Significance of difference | |||
---|---|---|---|---|---|---|---|---|---|---|
0.05 | 0.01 | 0.05 | 0.01 | 0.05 | 0.01 | |||||
0 | 12 | 3.33 | f | G | 2.67 | ef | F | 0.17 | e | E |
24 | 8.67 | f | G | 4.00 | ef | EF | 0.57 | e | E | |
36 | 5.33 | f | G | 2.00 | f | F | 0.25 | e | E | |
1 000 | 12 | 45.33 | d | EF | 11.33 | cd | CDE | 2.27 | c | CD |
24 | 49.33 | cd | DE | 13.33 | bcd | BCD | 2.72 | bc | C | |
36 | 54.67 | bc | DE | 10.67 | cd | CDE | 2.68 | bc | C | |
2 000 | 12 | 52.00 | bcd | DE | 10.67 | cd | CDE | 2.69 | bc | C |
24 | 70.00 | a | AB | 23.33 | a | A | 4.23 | a | A | |
36 | 54.67 | bc | DE | 14.00 | bcd | BCD | 2.95 | b | BC | |
3 000 | 12 | 59.33 | b | BCD | 18.00 | ab | ABC | 3.64 | a | AB |
24 | 72.00 | a | A | 21.33 | a | AB | 4.14 | a | A | |
36 | 52.67 | bcd | DE | 13.33 | bcd | BCD | 2.67 | bc | C | |
4 000 | 12 | 56.67 | bc | CDE | 14.67 | bc | BCD | 3.00 | b | BC |
24 | 67.33 | a | ABC | 20.67 | a | AB | 4.17 | a | A | |
36 | 37.33 | e | F | 8.00 | de | DEF | 1.70 | d | D |
表4 赤霉素浓度和浸种时间组合对下延叶古当归种子萌发变化
Tab.4 Effects of gibberellin concentration and soaking time combination on seed germination of A.decurrens
赤霉素浓度 Gibberellin concentration (mg/L) | 浸种时间 Seed soaking time (h) | 萌发率 Germin- ation rate (%) | 差异显著性 Significance of difference | 萌发势 Germination Potential (%) | 差异显著性 Significance of difference | 萌发指数 Germination index | 差异显著性 Significance of difference | |||
---|---|---|---|---|---|---|---|---|---|---|
0.05 | 0.01 | 0.05 | 0.01 | 0.05 | 0.01 | |||||
0 | 12 | 3.33 | f | G | 2.67 | ef | F | 0.17 | e | E |
24 | 8.67 | f | G | 4.00 | ef | EF | 0.57 | e | E | |
36 | 5.33 | f | G | 2.00 | f | F | 0.25 | e | E | |
1 000 | 12 | 45.33 | d | EF | 11.33 | cd | CDE | 2.27 | c | CD |
24 | 49.33 | cd | DE | 13.33 | bcd | BCD | 2.72 | bc | C | |
36 | 54.67 | bc | DE | 10.67 | cd | CDE | 2.68 | bc | C | |
2 000 | 12 | 52.00 | bcd | DE | 10.67 | cd | CDE | 2.69 | bc | C |
24 | 70.00 | a | AB | 23.33 | a | A | 4.23 | a | A | |
36 | 54.67 | bc | DE | 14.00 | bcd | BCD | 2.95 | b | BC | |
3 000 | 12 | 59.33 | b | BCD | 18.00 | ab | ABC | 3.64 | a | AB |
24 | 72.00 | a | A | 21.33 | a | AB | 4.14 | a | A | |
36 | 52.67 | bcd | DE | 13.33 | bcd | BCD | 2.67 | bc | C | |
4 000 | 12 | 56.67 | bc | CDE | 14.67 | bc | BCD | 3.00 | b | BC |
24 | 67.33 | a | ABC | 20.67 | a | AB | 4.17 | a | A | |
36 | 37.33 | e | F | 8.00 | de | DEF | 1.70 | d | D |
差异来源 Differences between the source | 萌发率Germination rate | |||
---|---|---|---|---|
df | MS | F | P | |
浓度 Concentration | 4 | 4 711.022 | 130.862 | 0.000 |
时间 Time | 2 | 663.822 | 18.440 | 0.000 |
浓度×时间 Concentration×Time | 8 | 172.489 | 4.791 | 0.001 |
表5 赤霉素浓度和浸种时间对下延叶古当归种子萌发率的双因素方差
Tab.5 Two-factor variance analysis of seed germination rate of A.decurrens with different gibberellin concentration and soaking time
差异来源 Differences between the source | 萌发率Germination rate | |||
---|---|---|---|---|
df | MS | F | P | |
浓度 Concentration | 4 | 4 711.022 | 130.862 | 0.000 |
时间 Time | 2 | 663.822 | 18.440 | 0.000 |
浓度×时间 Concentration×Time | 8 | 172.489 | 4.791 | 0.001 |
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