哈萨克母羊分娩前与胎儿组织器官细菌的多样性

doi:10.6048/j.issn.1001-4330.2021.05.021

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (5): 965-972.DOI: 10.6048/j.issn.1001-4330.2021.05.021

• 农产品分析检测·贮藏保鲜加工·草业·畜牧兽医 • 上一篇下一篇

哈萨克母羊分娩前与胎儿组织器官细菌的多样性

李晓斌, 马晨, 李凤鸣, 黄新新, 米热古丽·伊马木, 臧长江, 李佳豪, 李超, 马利鑫, 曹蕊

新疆农业大学动物科学学院,乌鲁木齐 830052

收稿日期:2020-05-13 出版日期:2021-05-20 发布日期:2021-05-26
通信作者: 臧长江(1977-),男,新疆昌吉人,副教授,硕士生导师,研究方向为动物营养与饲料科学,(E-mail)50954148@qq.com
作者简介:李晓斌(1988-),男,甘肃天水人,副教授,研究方向为草食动物营养代谢,(E-mail)172387243@qq.com
基金资助:
天池博士计划项目(2018XJTC0012);大学生创新项目

Study on Microbiota Diversity of Maternal and Fetus at the Late Pregnancy of Kazakh Ewe

LI Xiaobin,MA Chen, LI Fengming, HUANG Xinxin,Yimamu Mireguli, ZANG Changjiang, LI Jiahao,LI Chao, MA Lixin, CAO Rui

College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received:2020-05-13 Online:2021-05-20 Published:2021-05-26
Correspondence author: ZANG Changjiang(1977-),male, associate professor, master tutor, research direction is animal nutrition and feed science,(E-mail)50954148@qq.com.
Supported by:
Tianchi Doctoral Program Project (2018XJTC0012); University Student Innovation Project

摘要/Abstract

摘要： 【目的】研究分娩前哈萨克母羊和胎儿组织、肠道内容物及相关器官菌群的多样性。【方法】试验选择5只4岁、胎次相同、健康的妊娠后期母羊,平均体重为(55.64±4.37) kg,安乐死后,分离各组织器官并采集相关样品,用于分析菌群多样性。【结果】(1)母羊唾液、小肠内容物及羊水中有较高的多样性,chao1指数分别为1 042.02、1 058.31、1 011.22,ACE指数分别为1 069.18、1 067.41、1 014.17。(2)在物种分类水平上,母羊小肠、盲肠内容物、脐带及羔羊胎粪中厚壁菌门丰度较高,占比分别为79.08%、65.94%、49.66%和35.69%;在母羊唾液和羔羊瘤胃中拟杆菌门占比较高,分别为26.98和51.80%;羔羊瘤胃和母羊唾液中变形菌门的占比分别为64.92%和41.92%。(3)羔羊瘤胃液、胎粪及脐带中物种相似性较高,母羊唾液和瘤胃液中物种相似性较高,母羊小肠内容物和母羊盲肠内容物中物种相似性较高。【结论】母羊妊娠后期唾液、小肠内容物及羊水中有较高的多样性指数,厚壁菌门、变形菌门及拟杆菌门是此阶段母羊和胎儿各组织器官主要的物种。

关键词: 哈萨克母羊; 胎儿; 菌群结构; 多样性

Abstract: 【Objective】 The purpose of this experiment is to study the microbiota diversity of the flora of Kazakh ewe and fetal tissues, intestinal contents and related organs before delivery. 【Methods】 Five 4-year-old Kazakh ewe with average body weight of (55.64±4.37) kg and the same parity were selected. After euthanasia, tissue organ samples were isolated and collected and analyzed for bacterial diversity. 【Results】 (1) There were higher diversity of the saliva, smaller intestine contents and amniotic fluid in the ewes; the chao1 index were 1,042.02, 1,058.31, 1,011.22 and the ACE index were 1,069.18, 1,067.05, 1,014.17, respectively. (2)At the species classification level, the abundance of firmicutes was higher in the small intestine, cecal contents, umbilical cord and the ewe and lamb meconium, accounting for 79.08%, 65.94%, 49.66% and 35.69%, respectively. The bacteroides of saliva and lamb rumen was 26.98 and 51.80%, respectively. The proteobacteria of saliva and lamb rumen was 64.92% and 41.92%, respectively. (3)The results of aggregation showed that the similarity of the species in the rumen fluid, meconium and umbilical cord of the lamb was higher, the similarity of the species in the saliva of the ewes and the rumen fluid was higher, and the similarity of the contents of the small intestine of the ewes and the cecal contents of the ewe were higher. 【Conclusion】 Therefore, the saliva, small intestine contents and amniotic fluid of the ewes have higher diversity index in the late pregnancy, and the thick-walled bacteria, the proteobacteria and the bacteroides are the main species of the tissues and organs of the ewes and fetuses at this stage.

Key words: Kazakh ewes; fetus; flora structure; diversity

中图分类号:

S826

李晓斌, 马晨, 李凤鸣, 黄新新, 米热古丽·伊马木, 臧长江, 李佳豪, 李超, 马利鑫, 曹蕊. 哈萨克母羊分娩前与胎儿组织器官细菌的多样性[J]. 新疆农业科学, 2021, 58(5): 965-972.

LI Xiaobin,MA Chen, LI Fengming, HUANG Xinxin,Yimamu Mireguli, ZANG Changjiang, LI Jiahao,LI Chao, MA Lixin, CAO Rui. Study on Microbiota Diversity of Maternal and Fetus at the Late Pregnancy of Kazakh Ewe[J]. Xinjiang Agricultural Sciences, 2021, 58(5): 965-972.

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哈萨克母羊分娩前与胎儿组织器官细菌的多样性

Study on Microbiota Diversity of Maternal and Fetus at the Late Pregnancy of Kazakh Ewe

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