大蒜醇提液对小鼠离体小肠平滑肌运动的影响

doi:10.6048/j.issn.1001-4330.2019.11.020

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (11): 2133-2139.DOI: 10.6048/j.issn.1001-4330.2019.11.020

• 土壤肥料·林业·贮藏加工·植物保护·畜牧·农业经济 • 上一篇下一篇

大蒜醇提液对小鼠离体小肠平滑肌运动的影响

阿迪莱·艾力, 赵树林, 李璐璐, 孙小燕, 余万里, 郝智慧, 赵红琼

新疆农业大学动物医学学院,乌鲁木齐 830052

收稿日期:2019-06-28 出版日期:2019-11-20 发布日期:2019-12-09
通信作者: 赵红琼(1974-),女,四川省西充人,博士,研究方向为生物活性物质结构和功能,(E-mail)zhaohongqiong@sina.com
作者简介:阿迪莱·艾力(1996-),女,新疆人,研究方向为生物活性物质与功能,(E-mail)2171770758@qq.com
基金资助:
新疆维吾尔自治区科技支疆项目(2017E0242)

Effect of Alcohol Extract from Garlic on the Motility of Murine Small Intestinal Smooth Muscle in Vitro

Adilai Ali, ZHAO Shu-lin, LI Lu-lu, SUN Xiao-yan, YU Wan-li, HAO Zhi-hui, ZHAO Hong-qiong

College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Received:2019-06-28 Online:2019-11-20 Published:2019-12-09
Correspondence author: ZHAO Hong-qiong ( 1974 -), female, native place: Xichong, Sichuan, Associate professor; research field: The structure and function of biologically active substances, (E-mail)zhaohongqiong@sina.com
Supported by:
Supported by the Science and Techonology Supporting Xinjiang Project of Xinjiang Uygur Autonomous Region (2017E0242)

摘要/Abstract

摘要： 【目的】研究大蒜醇提液对胃肠运动的影响,了解其可能的作用机制。【方法】用生物信号处理系统监测大蒜醇提液对小鼠离体小肠平滑肌运动的影响。【结果】 2倍浓度递增(0.025%-0.4%)的大蒜醇提液剂量依赖性抑制小肠收缩幅度(P < 0.05),但对收缩张力无影响(P> 0.05);大蒜醇提液抑制小肠收缩幅度的最效浓度为0.2%,小肠收缩幅度仅为用药前26%±7%(P< 0.01);0.2%大蒜醇提液与M-受体激动剂氯贝胆碱或KCl联合用药对小肠收缩张力、收缩幅度、峰值和曲线下面积与这两种药物单独用药比差异均不显著(P> 0.05)。【结论】大蒜醇提液对小鼠离体小肠平滑肌的运动具有抑制作用,且该抑制作用可能同抑制胆碱能M-受体和阻止细胞内钾离子外流减少无关。

关键词: 大蒜; 醇提液; 小鼠; 小肠; M-受体; KCl

Abstract: 【Objective】 To explore the effect of alcohol extract from garlic on gastrointestinal motility and to understand its possible mechanism of action. 【Method】 In this experiment, the effect of alcohol extract from garlic on the motility of gastrointestinal smooth muscle in mice and its possible mechanism in vitro were monitored by biological signal processing system.【Result】 The results showed that garlic alcohol extract with twice concentration (0.025% - 0.4%) inhibited the contraction amplitude of small intestine (P< 0.05), but the contraction tension had not changed (P> 0.05); The maximum effective concentration of garlic was 0.2% which inhibited the contraction of intestine, and the contraction amplitude was 26% ± 7% of pre-administration (P< 0.01); 0.2% garlic combined with M-receptor agonist bethanechol chloride or KCl was not significantly different from bethanechol chloride or KCl alone in contraction tension, amplitude, peak and area under curve(P> 0.05). 【Conclusion 】 The present results indicate that alcohol extract from garlic inhibits the movement of isolated small intestine in mice, moreover, this inhibition effect may not be related to the inhibition of cholinergic M-receptors and the inhibition of decreased intracellular potassium ion outflow.

Key words: garlic; alcohol extract; mouse; small intestine; M-receptor; KCI

中图分类号:

S811

阿迪莱·艾力, 赵树林, 李璐璐, 孙小燕, 余万里, 郝智慧, 赵红琼. 大蒜醇提液对小鼠离体小肠平滑肌运动的影响[J]. 新疆农业科学, 2019, 56(11): 2133-2139.

Adilai Ali, ZHAO Shu-lin, LI Lu-lu, SUN Xiao-yan, YU Wan-li, HAO Zhi-hui, ZHAO Hong-qiong. Effect of Alcohol Extract from Garlic on the Motility of Murine Small Intestinal Smooth Muscle in Vitro[J]. Xinjiang Agricultural Sciences, 2019, 56(11): 2133-2139.

参考文献

[1] 马丽娜, 李峰杰, 陈坚, 等.大蒜主要活性成分及药理作用研究进展[J]. 中国药理学通报2014, 30(6) :760-763.
MA Li-na, LI Feng-jie, CHEN Jian, et al. Research advances in garlic’s main ingredients and their pharmacological effects [J] Chinese Pharmacological Bulletin, 2014, 30(6) :760-763.
[2] 胡玉熙, 陈曦, 刘清飞, 等.大蒜素药理作用研究的最新进展[J].药学进展,2007, (11) :481-485.
HU Yu-xi, CHEN Xi, LIU Qing-fei, et al. Recent Advances in Study of Pharmacological Action of Garlicin [J]. Progress in Pharmaceutical Sciences, 2007, (11) :481-485.
[3] 中国兽药典委员会. 中华人民共和国兽药典(第二部). [M].北京: 中国农业出版社, 2010 ,(3) .
Chinese Veterinary Pharmacopoeia Committee. Republic of China Veterinary Pharmacopoeia (Second part) [M]. Beijing: China Agriculture Press.2010 ,(3) .
[4] 彭敏. 大蒜素药理学作用的机制及研究进展[J]. 现代中西医结合杂志, 2018,27(14): 1593-1596.
PENG Min.Mechanism and research progress of pharmacological action of allicin [J]. Modern Journal of Integrated Traditional Chinese and Western Medicine, 2018,27(14): 1593-1596.
[5] 贺建忠, 王成江, 常娟, 等. 大蒜素在畜牧兽医中的应用[J]. 中国动物保健, 2007,(2): 77-78.
HE Jian-zhong, WANG Cheng-jiang, CHANG Juan, et al. Application of Allicin in Animal Husbandry and Veterinary [J]. China Animal Health, 2007,(2): 77-78.
[6] 张伟, 张爱忠, 姜宁, 等. 大蒜素对畜牧生产环境的保护作用[J]. 黑龙江畜牧兽医,2017,(16): 171-173.
ZHANG Wei, ZHANG Ai-zhong, JIANG Ning, et al. Protective effect of allicin on animal husbandry production environment [J]. Heilongjiang Animal Science and Veterinary Medicine, 2017,(16): 171-173.
[7] 于子洋, 王鑫, 林英庭. 大蒜素对动物血清生化指标的影响[J]. 粮食与饲料工业, 2013 , (9): 53-56.
YÜ Zi-yang, WANG Xin, LIN Ying-ting. Effect of allicin on Sevum biochemical parameters in animals [J]. Cereal & Feed Industry, 2013 , (9): 53-56.
[8] 王建平. 大蒜素对小鼠胃肠动力影响的实验研究[D]. 兰州:兰州大学硕士论文, 2015.
WANG Jian-ping. Experimental Study on the Effects of Garlicin on Gastrointestinal Motility [D]. Master Dissertation. Lanzhou University, Lanzhou. 2015.
[9] 杜汴兴, 唐引引, 岳静宇. 止泻方抗小鼠腹泻作用的实验研究[J]. 中医研究, 2013, 26(5): 78-80.
DU Bian-xing, TANG Yin-yin, YUE Jing-yu. Experimental Study on the antidiarrheal effect [J]. Traditional Chinese Medicinal Research, 2013, 26(5): 78-80.
[10] 徐淑云. 药理实验方法学(第3版)[M]. 北京: 北京人民卫生出版社, 2005.
XU Shu-yun. Pharmacological Experimental Methodology (Third Ed.) [M]. Beijing: Beijing People's Medical Publishing House.2005.
[11] 闫书平, 卡力比夏提·艾木拉江, 哈力旦·阿合买提, 等. 干鲜荨麻提取物对小鼠离体小肠运动影响的比较研究[J]. 动物医学进展, 2017, 38(9): 55-60.
YAN Shu-ping, Kailibixiati Aimulajiang, Halidan Ahemaiti, et al. Comparative Studies on Extracts of Fresh and Dried Urtica cannabina on Motilities of Mouse Small Intestine in Vitro [J].Progress in Veterinary Medicine2017,38(9): 55-60.
[12] 杨阳, 姚晓慧, 周玉东, 等. 辣椒、花椒和胡椒联合用药对小鼠离体小肠运动影响的研究[J]. 黑龙江畜牧兽医, 2016,(13): 197-200.
YANG Yang, YAO Xiao-hui, ZHOU Yu-dong, et al. Effect of co-administration of chili, wild pepper and black pepper on motility of the isolated Small intestine from mice [J]. Heilongjiang Animal Science and Veterinary Medicine, 2016,(13): 197-200.
[13] 张丹,石刚,周玉东,等.苦豆子植株不同部位醇提液对大鼠离体小肠运动的影响[J].中国畜牧兽医,2016,43(1):164-170.
ZHANG Dan, SHI Gang, ZHOU Yu-dong, et al. Effect of Alopecurordes on the motility of Isdated Rat Small Intestine [J]. China Animal Husbandry & Veterinary Medicine, 2016,43(1):164-170.
[14] 屈晞. 氯化钾在兽医临床上应用的体会[J]. 畜牧兽医杂志, 1987,(1): 51-52.
QU Xi. Experience in veterinary clinical application of Kcl [J]. Journal of Animal Science and Veterinary Medicine, 1987,(1): 51-52.
[15] 马晓莉, 曹松云, 王淼, 等. 大蒜硝黄膏穴位贴敷治疗肠梗阻的药理作用研究[J]. 中成药, 2012, 34(3): 564-566.
MA Xiao-li, CAO Song-yun, WANG Miao, et al. Study on Pharmacological Action of Acupoint Application of Garlic Nitrate Cream in Treating Intestinal Obstruction [J]. Chinese Traditional Patent Medicine, 2012, 34(3): 564-566.
[16] 周杰. 动物生理学[M]. 北京: 中国农业大学出版社, 2018.
ZHOU Jie. Animal physiology [M]. Beijing: China Agricultural University Press. 2018.
[17] 里斯(Reece,W.O.), 赵茹茜译.DUKES家畜生理学(第12版/美)[M]. 北京: 中国农业出版社, 2014.
Reece, W. O. Dukes' physiology of domestic animals (12th Ed./USA.) Translated by ZHAO Ru-qian [M]. Beijing: China Agriculture Press. 2014.

大蒜醇提液对小鼠离体小肠平滑肌运动的影响

Effect of Alcohol Extract from Garlic on the Motility of Murine Small Intestinal Smooth Muscle in Vitro

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