不同产地及品种大蒜内生菌群落多样性分析及拮抗作用测定

doi:10.6048/j.issn.1001-4330.2017.11.014

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (11): 2067-2074.DOI: 10.6048/j.issn.1001-4330.2017.11.014

不同产地及品种大蒜内生菌群落多样性分析及拮抗作用测定

楚敏¹, 张丽娟¹, 刘晓静², 朱静¹, 王玮¹, 顾美英¹, 谢玉清¹, 张志东¹

1.新疆农业科学院微生物应用研究所,乌鲁木齐 830091;
2.新疆农业大学食品科学与药学学院,乌鲁木齐 830052

收稿日期:2017-08-18 出版日期:2017-11-20 发布日期:2017-12-06
通信作者: 张志东(1977-),男,新疆乌鲁木齐人,研究员,硕士,研究方向为微生物生态,(E-mail)zhangzheedong@sohu.com
作者简介:楚敏(1977-),女,新疆乌鲁木齐人,副研究员,硕士,研究方向为分子生物学与微生物学,(E-mail)chuliu2002@163.com
基金资助:
新疆维吾尔自治区自然基金项目“新疆大蒜根腐病病原菌分离鉴定及发病规律动态分析”(2014211B033)

Diversity of the endophytes and screening of antagonistic microbes in Garlic varieties from different producing areas

CHU Min¹, ZHANG Li-juan¹, LIU Xiao-Jing², ZHU Jing¹, WANG Wei¹, GU Mei-ying¹, XIE Yu-qing¹, ZHANG Zhi-dong¹

1.Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091;
2. Department of Food Science, College of Food Science and Pharmaceutical Science, Xinjiang Agricultural University, Urumqi 830052

Received:2017-08-18 Online:2017-11-20 Published:2017-12-06
Correspondence author: ZHANG Zhi-dong(1977-), male, native place:Urumqi,Xinjiang, researcher, Master,research field:Microbial ecology, (E-mail)zhangzheedong@sohu.com
Supported by:
Natural Science Funds of Xinjiang Uygur Autonomous Region (No.2014211B033)

摘要/Abstract

摘要： 【目的】调查分析不同产地及不同品种大蒜内生菌的群落多样性,测定内生菌对5株病原菌的拮抗作用。【方法】以新疆、山东的白皮蒜、红皮蒜为研究材料,采用BIOLOG生态板法和培养基培养法进行大蒜内生菌群落多样性的分析,并利用平板对峙法,利用大蒜内生菌对立枯丝核菌(Rhizoctonia soleni)、棉花枯萎病菌棉花枯萎病菌 (Fusarium oxysporumf)、扩展青霉 (Penicillium expansum)、大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphyloccocus aureus)的拮抗作用进行研究。【结果】不同品种来源的大蒜内生菌群落代谢活性均较低,但不同类型碳源利用活性存在明显分布规律;初步筛选获得的45株内生菌,分属于芽孢杆菌属(Bacillus)、类芽孢杆属(Paenibacillus)、链霉菌属(Streptomyces)、拟诺卡氏菌属(Nocardiopsis) 和迪茨菌属(Dietzia) 等5个属15个种,其中芽孢杆菌属菌株占绝大部分,占85%以上,且在不同大蒜样品中均为优势菌,但种间分布存在着差异。获得了24株具有拮抗作用的内生菌菌株。【结论】大蒜内生菌群落多样性较为简单,存在着具有拮抗作用的微生物资源,有待进一步开发利用。

关键词: 大蒜品种; 不同产地; 内生菌; 拮抗菌

Abstract: 【Objective】 In order to investigate diversity of the endophytes in Garlic varieties from different producing area, and obtain antagonistic microbes.【Method】Garlic varieties, white garlic and red garlic from different producing areas were sampled. And then, Biolog Eco method was employed to investigate metabolic characteristics of endophytes. Meanwhile, Endophytes were isolated and antagonistic bacteria to Rhizoctonia soleni,Fusarium oxysporumf,Penicillium expansum,Escherichia coli and Staphyloccocus aureus were screened.【Result】Low metabolic activity of endophytes in Garlic were determined, and some differences in the utilization and diversity in Garlic varieties from different producing areas were analyzed. 45 endophytes were isolated which were involved in 15 species belonging to genus Bacillus, genus Paenibacillus , genus Streptomyces , genus Nocardiopsis , and genus Dietz . Isolates in Genus Bacillus were dominant in Garlic endophytes, accounted for more than 85%, but there were differences in the distribution of species. Additionally, lots of antagonistic bacteria were obtained. 【Conclusion】Diversity of the endophytes in Garlic varieties from different producing area were all poor, but effective resource of antagonistic microbes needs further explore.

Key words: Garlic varieties; different producing areas; endophytes; antagonistic microbes

中图分类号:

S188

楚敏, 张丽娟, 刘晓静, 朱静, 王玮, 顾美英, 谢玉清, 张志东. 不同产地及品种大蒜内生菌群落多样性分析及拮抗作用测定[J]. 新疆农业科学, 2017, 54(11): 2067-2074.

CHU Min, ZHANG Li-juan, LIU Xiao-Jing, ZHU Jing, WANG Wei, GU Mei-ying, XIE Yu-qing, ZHANG Zhi-dong. Diversity of the endophytes and screening of antagonistic microbes in Garlic varieties from different producing areas[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2067-2074.

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不同产地及品种大蒜内生菌群落多样性分析及拮抗作用测定

Diversity of the endophytes and screening of antagonistic microbes in Garlic varieties from different producing areas

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