新疆农业科学 ›› 2021, Vol. 58 ›› Issue (4): 731-740.DOI: 10.6048/j.issn.1001-4330.2021.04.016

• 土壤肥料·植物保护·微生物 • 上一篇    下一篇

盐穗木叶片及根际土壤微生物群落高通量分析

迪拉热·海米提, 樊永红, 王伟楠, 喻文丽, 艾海白尔·卡斯木   

  1. 新疆大学生命科学与技术学院,乌鲁木齐 830046
  • 收稿日期:2020-03-10 出版日期:2021-04-20 发布日期:2021-04-25
  • 通信作者: 樊永红(1974- ),女,甘肃静宁人,副教授,博士,硕士生导师,研究方向为环境微生物,(E-mail) yhfanzyb2004@163.com
  • 作者简介:迪拉热·海米提(1995- ),女,新疆克拉玛依人,硕士研究生,研究方向为微生物学,(E-mail)599181978@qq.com
  • 基金资助:
    国家自然科学基金“新疆盐穗木根际ACC脱氨酶耐盐碱菌株的筛选及促生作用的研究”(31560032)

Analysis of Microbial Communities in Leaves and Rhizosphere Soil of Halostachys capsica by High-throughput Sequencing

Dilare Haimiti, FAN Yonghong, WANG Weinan, YU Wenli, Aihaibaier Kasimu   

  1. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
  • Received:2020-03-10 Online:2021-04-20 Published:2021-04-25
  • Correspondence author: FAN Yonghong(1974-),female, native place:Jingning, Gansu. Associate Professor, research field: Environmental microbiology, (E-mail)yhfanzyb2004@163.com
  • Supported by:
    National Natural Science Foundation of China "Research on Screening Salt-tolerant strain of ACC deaminase from Halostachys caspica rhizosphere soil in Xinjiang and Its Promoting Effects on Plant Growth" (31560032)

摘要: 【目的】 研究新疆盐碱环境中,极端耐盐植物盐穗木的根际土壤及叶片内生微生物群落组成和结构,为利用根际促生细菌制作生物菌肥提供科学依据。【方法】 应用16S rDNA和ITS高通量测序技术,测定分析盐穗木根际土壤与叶片内生微生物群落结构及多样性。【结果】 在门水平上根际土壤细菌群落中的优势菌门为放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、芽单胞菌门(Gemmatimonadetes)等。根际盐穗木真菌群落中的优势菌门为子囊菌门(Ascomycota)。在门水平下,盐穗木叶片内生细菌群落中的优势菌门为蓝细菌门(Cyanobacteria)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria);内生真菌群落中的优势菌门为子囊菌门(Ascomycota)。Alpha 多样性指数分析得出,在3 个土壤样品中,YS1的Shannon,Simpson指数最高,即微生物群落多样性较其余2个样品高。【结论】 在3个叶片样品中,YP3的Shannon,Simpson等指数最高,即微生物群落多样性较其余2个样品高。盐穗木根际土壤微生物群落多样性高于叶片内生微生物群多样性。

关键词: 盐穗木; 根际微生物; 内生菌; 微生物群落多样性; 高通量测序

Abstract: 【Objective】 To explore the rhizosphere soil and endophytic microbial community composition and structure of the extremely salt-tolerant plant Halostachys capsica in the saline-alkali environment of Xinjiang. 【Methods】 16 S rDNA and ITS high throughput sequencing technology were used in this project to determine and analyze the structure and diversity of endophytic microbial community in rhizosphere soil and leaves of salt panicle. 【Results】 The results showed that the dominant bacteria in the rhizosphere soil bacterial community at the level of the gate were Actinobacteria, Proteobacteria, and Gemmatimonadetes. The dominant bacterium in the rhizosphere was Ascomycota. At the level of the gate, the dominant bacteria in the endophytic bacterial community of the leaves of the leaves were Cyanobacteria, Actinobacteria, Proteobacteria, and a dominant bacterium in endophytic fungi was Ascomycota. According to the Alpha diversity index analysis, the Shannon and Simpson indices of YS1 were the highest in the three soil samples. Namely, the microbial community diversity was higher than the other two samples. 【Conclusion】 Among the three leaf samples, the Shannon, Simpson and other indexes of YP3 were the highest, that was, the microbial community diversity was higher than the other two samples. The microbial community diversity in the rhizosphere soil of Halostachys capsica was higher than that in the endogenous microbial community. To sum up, the analysis of microbial community composition in rhizosphere soil and leaves of Halostachys capsica provides scientific basis for further study of the correlation between microbial community and its resistance to stress in arid and saline-alkali habitats by PGPR.

Key words: Halostachys capsica; rhizosphere microorganism; endophytic bacteria; microbial community diversity; high-throughput sequencing

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

出版单位:《新疆农业科学》编辑部
地址:乌鲁木齐市南昌路403号新疆农业科学院
邮编:830091
电话:0991-4502046
E-mail:xjnykx-h@xaas.ac.cn


版权所有 © 《新疆农业科学》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量: 今日访问: 在线人数:
网站
微信公众号
淘宝购买
微店购买