PPRV中国新疆南疆株F基因遗传演化分析

doi:10.6048/j.issn.1001-4330.2017.11.020

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (11): 2118-2129.DOI: 10.6048/j.issn.1001-4330.2017.11.020

PPRV中国新疆南疆株F基因遗传演化分析

张路瑶^1,2, 李飞^2,3, 焦海宏², 赵丽², 刘永宏²

1.巴里坤哈萨克自治县畜牧兽医工作站,新疆哈密 839200;
2.塔里木大学动物科学学院/新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔 843300;
3. 新疆阿克苏地区动物疫病控制诊断中心,新疆阿克苏 843000

收稿日期:2017-08-24 出版日期:2017-11-20 发布日期:2017-12-06
通信作者: 刘永宏(1981-),男,内蒙古武川人,副教授,博士,研究方向为传染病与免疫病理学,(E-mail)lyhdky@126.com
作者简介:张路瑶(1991-),男,陕西礼泉人,硕士,研究方向为动物群发性疾病的防控检测,(E-mail)875793428@qq.com
基金资助:
华中农业大学塔里木大学科研联合基金“新疆南疆小反刍兽疫流行病学调查”(HNTDLH1404)

Genetic Evolution Analysis of F gene of PPRV in Southern Xinjiang

ZHANG Lu-yao^1,2, LI Fei^2,3, JIAO Hai-hong², ZHAO Li², LIU Yong-hong²

1.nimal Husbandry and Veterinary Workstations of Barkol Kazak Autonomous County, Kumul Xinjiang 839200, China;
2. College of Animal Science, Tarim University, Key Laboratory of Tarim Animanl Husbandry Science and Technology of Xinjiang Production & Construction Corps, Alar Xinjiang 843300 China;
3.Aksu Animal Loimia Controlling and Diagnostic Center, Aksu Xinjiang 843300, China

Received:2017-08-24 Online:2017-11-20 Published:2017-12-06
Correspondence author: LIU Yong-hong(1981-), male, native place: Wuchuan Inner Mongolia, Associate professor, doctoral, The research direction for infectious diseases and immune pathology, (E-mail)lyhdky@126.com
Supported by:
Tarim University and Huazhong Agricultural University Scientific Research Funds "Peste-des-petits Ruminants Epidemiological Investigation in Southern Xinjiang"(HNTDLH1404)

摘要/Abstract

摘要： 【目的】分析中国新疆南疆2014年PPRV毒株来源,为中国新疆南疆PPR防控提供理论依据。【方法】调查中国新疆南疆阿克苏地区某羊场发病羊发病情况、临床症状观察及病理学检查,实验室进行PPRV F基因RT-PCR和F基因测序,进一步对PPRV F基因序列分析。【结果】毒株与国内PPRV毒株F基因核苷酸同源性最高的是中国新疆China/XJYL/2013毒株,与国外的毒株F基因核苷酸同源性最高的是印度Izatnagar/94毒株及Izatnagar-94毒株;此次毒株与国内PPRV毒株F蛋白氨基酸同源性最高的是中国新疆China/XJYL/2013毒株,与国外的PPRV毒株F蛋白氨基酸同源性最高的是印度Izatnagar/94毒株。PPRV毒株F基因进化树分析显示:PPRV毒株与北京PPRV毒株进化关系最近,与中国新疆伊犁PPRV毒株同属一个分支,与国外印度及孟加拉国毒株的进化关系较近。【结论】2014年中国新疆南疆存在PPR疫情,该疫情毒株与此次疫情前后国内的PPR毒株及印度PPR毒株进化关系较近。

关键词: 小反刍兽疫; 诊断; F基因; 序列分析

Abstract: 【Objective】 In order to provide a theoretical basis for the prevention and control of PPR in southern Xinjiang, the PPRV strains in 2014 was analyzed.【Method】The incidence of sheep in a sheep farm in Akesu Prefecture of southern Xinjiang was investigated. The clinical symptoms and pathological examination were performed. The PPRV F gene RT-PCR and F gene sequencing were carried out in the laboratory, and the sequence analysis of PPRV F gene was further carried out.【Result】PPRV F gene sequence analysis showed that the test China/XJYL/2013 in Xinjiang had the highest homology with domestic PPRV strainF gene nucleotide. Compared with the Foreign strains, the highest homology of nucleotide is India strains Izatnagar/94 and Izatnagar-94 strains; PPRV strain F gene phylogenetic tree analysis in this study showed that the relationship of this PPRV strain had recently evolved from Beijing PPRV strain and belonged to the same branch of Ili PPRV strain, which had close evolutionary relationship with foreign strains in India and Bangladesh.【Conclusion】There existed PPR epidemic in southern Xinjiang in 2014, and the epidemic strains and the domestic PPR before and after the outbreak of the PPR strains and the surrounding countries strains were relatively close to the evolution.

Key words: PPR; diagnosis; F gene; sequence analysis

中图分类号:

S852.6

张路瑶, 李飞, 焦海宏, 赵丽, 刘永宏. PPRV中国新疆南疆株F基因遗传演化分析[J]. 新疆农业科学, 2017, 54(11): 2118-2129.

ZHANG Lu-yao, LI Fei, JIAO Hai-hong, ZHAO Li, LIU Yong-hong. Genetic Evolution Analysis of F gene of PPRV in Southern Xinjiang[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2118-2129.

参考文献 32

[1]	吴国珍.西藏小反刍兽疫疫情流行及防控对策研究[D].扬州:扬州大学硕士学位论文, 2010.WU Guo-zhen.(2010). Study on the prevalence and control measures of Tibet Peste des Petits Ruminants[D]. Master Thesis. Yangzhou University, Yangzhou. (in Chinese)
[2]	Banyard, A. C., Parida, S., Batten, C., Oura, C., Kwiatek, O., & Libeau, G. (2010). Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control. Journal of General Virology, 91(Pt 12): 2,885-2,897.
[3]	Elzein, E. A., Hassanien, M. M., Alafaleq, A. I., Abd Elhadi, M. A., & Housawi, F. M. (1990). Isolation of peste des petits ruminants from goats in saudi arabia. Veterinary Record, 127(12):309-310.
[4]	Kumar, N., Maherchandani, S., Kashyap, S. K., Singh, S. V., Sharma, S., & Chaubey, K. K., et al. (2014). Peste des petits ruminants virus infection of small ruminants: a comprehensive review. Viruses, 6(6): 2,287-2,327.
[5]	Taylor, W. P., Al, B. S., & Barrett, T. (1990). The epidemiology of peste des petits ruminants in the sultanate of oman. Veterinary Microbiology,22(4): 341-352.
[6]	Obi, T. U., Rowe, L. W., & Taylor, W. P. (1984). Serological studies with peste des petits ruminants and rinderpest viruses in nigeria. Tropical Animal Health & Production, 16(2):115-118.
[7]	Balamurugan, V., Saravanan, P., Sen, A., Rajak, K. K., Venkatesan, G., & Krishnamoorthy, P., et al. (2012). Prevalence of peste des petits ruminants among sheep and goats in india. Journal of Veterinary Science, 13(3): 279-285.
[8]	Anees, M., Shabbir, M. Z., Muhammad, K., Nazir, J., Shabbir, M. A. B., & Wensman, J. J., et al. (2013). Genetic analysis of peste des petits ruminants virus from pakistan. Bmc Veterinary Research, 9(1):1-5.
[9]	Amjad, H., Qamar-ul-Islam, Forsyth, M., Barrett, T., & Rossiter, P. B. (1996). Peste des petits ruminants in goats in pakistan. Veterinary Record, 139(5): 118-119.
[10]	王志亮, 包静月, 吴晓东, 等. 我国首例小反刍兽疫诊断报告[J]. 中国动物检疫, 2007, 24(8): 24-26.WANG Zhi-liang, BAO Jing-yue, WU Xiao-dong, et al.(2007). The first report on the diagnosis of peste des petits ruminants in China [J]. Chinese Journal of Animal Health Inspection, 24(8): 24-26. (in Chinese)
[11]	次仁罗布. 西藏阿里地区小反刍兽疫的流行情况与综合防控[J]. 山东畜牧兽医, 2013, 34(5): 44-45.Cirenluobu.(2013). The epidemic situation and comprehensive prevention and control of Tibet Ali region of peste des petits ruminants [J]. Shandong Journal of Animal Science and Veterinary Medicine, 34(5): 44-45. (in Chinese)
[12]	Bao, J., Wang, Z., Li, L., Wu, X., Sang, P., & Wu, G., et al. (2011). Detection and genetic characterization of peste des petits ruminants virus in free-living bharals (pseudois nayaur) in Tibet, China. Research in Veterinary Science, 90(2): 238-240.
[13]	王乐元, 次真, 吴国珍, 等. 中国西藏小反刍兽疫的发生状况与防控[J]. 畜牧兽医学报, 2011, 42(5): 717-720.WANG Le-yuan, CI Zhen, WU Guo-zhen, et al.(2011). Occurrence and control strategy of peste des petits ruminants in Tibet autonomous region of China [J]. Acta Veterinaria et Zootechnica Sinica, 42(5): 717-720. (in Chinese)
[14]	吴锦艳, 尚佑军, 田宏,等. 2007-2014年国内外小反刍兽疫流行现状及分析[J]. 中国兽医学报, 2016, 36(4): 687-693.WU Jin-yan, SHANG You-jun, TIAN Hong, et al.(2016). The epidemic situation and analysis of peste des petits ruminants in China and worldwide from 2007 to 2014[J]. Chinese Journal of Veterinary Science, 36(4): 687-693. (in Chinese)
[15]	Bao, J., Wang, Q., Zhang, Y., Liu, C., Li, L., & Wang, Z. (2014). Complete genome sequence of a novel variant strain of peste des petits ruminants virus, china/xjyl/2013. Genome Announcements, 2(5):65-73.
[16]	中华人民共和国农业部. 10月16日全国小反刍兽疫确诊疫情情况[EB/OL]. http://www.syj.moa.gov.cn/dwyqdt/qt/201610/t20161018_5308509.htm.Ministry of Agriculture of the People's Republic of China. (2016). Epidemic situation of confirmed ruminants in China [EB/OL]. http://www.syj.moa.gov.cn/dwyqdt/qt/201610/t20161018_5308509.htm.
[17]	杨香坊. 小反刍兽疫病毒F蛋白与H蛋白相互作用及F蛋白免疫原性研究[D]. 兰州: 甘肃农业大学硕士学位论文, 2013.YANG Xiang-Fang.(2013). The study of F protein immunogenicity and interaction of F and H protein of PPRV[D]. Master Thesis. GanSu Agricultural University, Lanzhou. (in Chinese)
[18]	Brindha, K., Raj, G. D., Ganesan, P. I., Thiagarajan, V., Nainar, A. M., & Nachimuthu, K. (2001). Comparison of virus isolation and polymerase chain reaction for diagnosis of peste des petits ruminants. Acta Virologica, 45(3):169-172.
[19]	Rahman, M. M., Shaila, M. S., & Gopinathan, K. P. (2003). Baculovirus display of fusion protein of peste des petits ruminants virus and hemagglutination protein of rinderpest virus and immunogenicity of the displayed proteins in mouse model. Virology, 317(1):36-49.
[20]	王春霞. 一起小反刍兽疫的诊断及病原分离鉴定[D]. 长春: 吉林大学硕士学位论文, 2015.WANG Chun-xia.(2015). Diagnosis of peste des petits ruminants and identification of peste des petits ruminants virus[D]. Master Thesis. Jilin University, Changchun. (in Chinese)
[21]	Rowland, A. C., Scott, G. R., Ramachandran, S., & Hill, D. H. (1971). A comparative study of peste des petits ruminants and kata in west african dwarf goats. Tropical Animal Health & Production, 3(4): 241-247.
[22]	Brown, C. C., Mariner, J. C., & Olander, H. J. (1991). An immunohistochemical study of the pneumonia caused by peste des petits ruminants virus. Veterinary Pathology, 28(2):166-170.
[23]	王光祥, 贾宁, 方梅, 等. 绵羊小反刍兽疫临床病例的病理学观察[J]. 畜牧兽医学报, 2015, 46(6): 1 011-1 017.WANG Guang-xiang, JIA Ning, FANG Mei, et al.(2015) Studies on the pathology of the peste des petits ruminants of sheep [J]. Acta Veterinaria et Zootechnica Sinica, 46 (6): 1,011-1,017. (in Chinese)
[24]	Singh, B., Bardhan, D., Verma, M. R., Prasad, S., & Sinha, D. K. (2014). Estimation of economic losses due to peste de petits ruminants in small ruminants in india. Veterinary World, 7(4):194-199.
[25]	王清华, 刘春菊, 吴晓东,等.新疆小反刍兽疫疫情诊断[J]. 中国动物检疫, 2014, 31(1): 72-75.WANG Qing-hua, LIU Chun-ju, WU Xiao-dong, et al.(2014). Diagnosis of peste des petits ruminants in goats in Xinjiang China [J]. China Animal Health Inspection, 31(1): 72-75. (in Chinese)
[26]	包静月, 王志亮, 李林, 等.我国西藏小反刍兽疫病毒China/Tib/Gej/07-30核衣壳蛋白基因和基因组启动子区的分子特征分析[J]. 病毒学报, 2008, 24(6): 464-471.BAO Jing-yue, WANG Zhi-liang, LI Lin, et al (2008).Sequence analysis of the nucleocapsid gene and genome promoter region of peste des petits ruminants virus of Chinese origin [J]. Chinese Journal of Virology, 24(6): 464-471. (in Chinese)
[27]	Mahapatra, M., Parida, S., Baron, M., & Barrett, T. (2006). Matrix protein and glycoproteins f and h of peste-des-petits-ruminants virus function better as a homologous complex. Journal of General Virology, 87(7): 2,021-2,029.
[28]	Shaila, M. S., Shamaki, D., Forsyth, M. A., Diallo, A., Goatley, L., & Kitching, R. P., et al. (1996). Geographic distribution and epidemiology of peste des petits ruminants viruses. Virus Research, 43(2):149-153.
[29]	Kwiatek, O., Minet, C., Grillet, C., Hurard, C., Carlsson, E., & Karimov, B., et al. (2007). Peste des petits ruminants (ppr) outbreak in tajikistan. Journal of Comparative Pathology, 136(2-3):111-119.
[30]	刘永宏, 曹胜波, 赵丽, 等.中国小反刍兽疫疫情分析[J]. 西北农业学报, 2014, 2(9): 19-26.LIU Yong-hong, CAO Sheng-bo, ZHAO Li, et al.(2014). Analysis of epidemic Chinese peste des petits ruminants [J]. Acta Agriculturae Aoreali-occidentalis Ainica, 2(9): 19-26. (in Chinese)
[31]	阮洋. 小反刍兽疫病毒F、H基因的核酸疫苗及N蛋白的原核表达研究[D]. 长春: 吉林农业大学硕士学位论文, 2011.RUAN Yang.(2011). Experimental study on DNA vaccine of PPRV F H gene and prokaryotic expression of N gene[D]. Master Thesis. Jilin Agricultural University, Changchun. (in Chinese)
[32]	罗静, 何宏轩. 小反刍兽疫病毒的分子生物学特性及其在全球的流行[J]. 河北师范大学学报, 2009, 4(33): 543-549.LUO Jing, HE Hong-xuan.(2009). The molecular biological characterization of peste des petits ruminants virus and its prevalence in the world [J]. Journal of Hebei Normal University, 4(33): 543-549. (in Chinese)

PPRV中国新疆南疆株F基因遗传演化分析

Genetic Evolution Analysis of F gene of PPRV in Southern Xinjiang

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献 32

相关文章 15

编辑推荐

Metrics

[1]	杨川, 张凯, 陈冰, 张慧, 柳萍, 常松, 盛建东. 棉花植株形态特征对不同水分状况的响应[J]. 新疆农业科学, 2023, 60(9): 2120-2127.
[2]	靳娟, 苏比娜·肖克来提, 阿布都卡尤木·阿依麦提, 杨磊, 郝庆, 樊丁宇. 灰枣扩展蛋白基因ZjEXPA8的克隆及序列分析[J]. 新疆农业科学, 2023, 60(9): 2223-2230.
[3]	王曼, 张政, 伊丽达娜·迪力夏提, 吴斌. 木纳格葡萄谷胱甘肽-S-转移酶VvGST1基因克隆与序列分析[J]. 新疆农业科学, 2023, 60(8): 1922-1930.
[4]	娜斯拜·阿卜杜瓦哈普, 高晓娟, 坤杜孜阿依·阿布都沙拉木, 李江伟. 新疆双峰驼C3d基因cDNA的克隆与序列分析[J]. 新疆农业科学, 2023, 60(2): 493-500.
[5]	李丰, 赖刚刚, 赵志惠, 陈小飞, 朱天生. 柽柳丛枝植原体鉴定及16S rRNA基因多样性分析[J]. 新疆农业科学, 2023, 60(10): 2551-2557.
[6]	刘丽娅, 叶锋, 马晓菁, 谷文喜, 谢彩云, 钟旗, 易新萍. 布鲁氏菌病不同初筛方法及诊断试剂检测差异性分析[J]. 新疆农业科学, 2022, 59(1): 215-222.
[7]	王重, 樊哲儒, 张跃强, 李剑峰, 高新, 时佳. 小麦叶绿素酸酯a氧化酶(TaCAO)基因的克隆与表达分析[J]. 新疆农业科学, 2021, 58(9): 1569-1576.
[8]	谭京红, 吴启侠, 朱建强, 佘子浩, 柯鑫瑶, 马泓雨. 麦后移栽棉氮素营养诊断与追肥模型[J]. 新疆农业科学, 2021, 58(7): 1225-1235.
[9]	杨波, 车玉红, 郭春苗, 木巴热克·阿尤普, 吴津蓉, 杜鹃. 榅桲4CL基因的克隆与生物信息学分析[J]. 新疆农业科学, 2021, 58(6): 1055-1063.
[10]	车玉红, 杨波, 郭春苗, 木巴热克·阿尤普, 吴津蓉, 杜鹃. 榅桲果实CAD基因的克隆、序列分析及表达[J]. 新疆农业科学, 2020, 57(5): 814-821.
[11]	朱宗财, 张王斌, 李亚鹏, 严海璘, 仪子博. 新疆果园不同寄主腐烂病菌交互侵染研究[J]. 新疆农业科学, 2019, 56(6): 1112-1121.
[12]	郑文强, 岳春芳, 曹伟. 基于冠气温差的阿克苏成龄红枣缺水诊断及预测[J]. 新疆农业科学, 2019, 56(12): 2256-2262.
[13]	张校立,徐叶挺,艾沙江·买买提,许娟,邓莉,王继勋. 新疆地方梨品种S基因型的鉴定[J]. 新疆农业科学, 2018, 55(2): 246-252.
[14]	孙亮,文凤,刘峰,张新宇,孙杰. 棉花GhFAD2-1基因5’UTR内含子的克隆与序列分析[J]. 新疆农业科学, 2018, 55(1): 1-8.
[15]	吴建勇;吴星星;杨学云;李建军;王登峰;金映红;王光雷;王治才. 绵羊副结核病的病原学检测与分析[J]. , 2016, 53(8): 1562-1568.