中国新疆部分地区马泰勒虫感染的差异性及系统发育分析

doi:10.6048/j.issn.1001-4330.2017.11.021

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (11): 2130-2137.DOI: 10.6048/j.issn.1001-4330.2017.11.021

中国新疆部分地区马泰勒虫感染的差异性及系统发育分析

刘世芳¹, 郭庆勇¹, 闻秀秀¹, 张梦园¹, 党娜娜², 阿拉西·阿滨³, 巴音查汗¹

1.新疆农业大学动物医学学院, 830052;
2.和静畜牧兽医站,新疆和静 841300;
3.昭苏县畜牧兽医站,新疆昭苏 835604

收稿日期:2017-09-14 出版日期:2017-11-20 发布日期:2017-12-06
通信作者: 巴音查汗(1964-),女,新疆巴州人,教授,研究方向为寄生虫免疫学及原虫分子生物学,(E-mail)bynch@hotmail.com,2514062881@qq.com
作者简介:刘世芳(1990-),男,云南曲靖人,硕士研究生,研究方向为临床兽医学,(E-mail)727640930@qq.com
基金资助:
国家自然科学基金项目-新疆联合基金重点项目“新疆马梨形虫病流行规律及其优势媒介蜱生物学特性和检测技术的研究”(U1403283);新疆农业大学研究生科研创新项目课题“马泰勒虫病中西药物效果筛选及治疗”(XJAUGRI2016-009)

Differences in Theileria equi Infection and Its Phylogenetic Development Analysis in Some Areas of Xinjiang

LIU Shi-fang¹, GUO Qing-yong¹, WEN Xiu-xiu¹, ZHANG Meng-yuan¹, DANG Na-na², Alax Abing³, Bayinchahan¹

1.College of Animal Medicine, Xinjiang Agricultural University, Urumqi 830052, China;
2. Hejing Animal Husbandry and Veterinary Station, Hejing Xinjiang 841300, China;
3. Zhaosu Animal Husbandry and Veterinary Station, Zhaosu Xinjiang 835604, China

Received:2017-09-14 Online:2017-11-20 Published:2017-12-06
Correspondence author: Bayinchahan(1964-),female,Bazhou,Xinjiang,Master. research field:Parasitic immunology and molecular biology of protozoa. (E-mail)bynch@hotmail.com,2514062881@qq.com
Supported by:
National Natural Science Foundation-Xinjiang Joint Fund Key Projects"The EquinePiroplasmosis Epidemic Regularity and Advantages of Ticks in Xinjiang: Biological Characteristics and Detection Techniques of Vectors Research" (U1403283)and Research and Innovation Projects of Graduate Students of Xinjiang Agricultural University "Screening and treatment of Chinese and Western medicine for Theileriosis Equi" (XJAUGRI2016-009)

摘要/Abstract

摘要： 【目的】分析新疆南北疆马泰勒虫感染的差异性及地方流行株遗传进化距离,研究马泰勒虫遗传多样性及其感染率。【方法】采自南北疆478份疑似马匹的血样,经马泰勒虫PCR方法检测,分析马泰勒虫感染情况并应用最大似然法(ML)基于18 S rRNA基因的遗传进化树及构建系统发育树。【结果】在所采集的样品中,中国新疆北疆地区马泰勒虫感染阳性率为13.96%(25/179),中国新疆南疆地区马泰勒虫感染阳性率为27.09%(78/299)。印度、南非、西班牙、伊朗等地方株聚为一支,扩增的18S rRNA基因(MF398476、MF398477)与瑞典地方株聚为一支。【结论】中国新疆南疆马泰勒虫感染率高于北疆,南北疆马泰勒虫感染存在显著性差异(P=0.001<0.05),在不同年龄段马匹的感染无显著性差异(P>0.05);扩增的马泰勒虫阿勒泰、托克逊地方流行株(MF398477、MF398476)与瑞士地方株(KM046918.1)亲缘关系最近。

关键词: 马泰勒虫; 感染; 系统发育

Abstract: 【Objective】 To understand the situation of Theileria equi and the genetic distance of local endemic strains strain of Theileria equi in Xinjiang.【Method】478 horse blood samples were collected from Southern and Northern Xinjiang, and Equine Theileriosis was investigated by PCR, the 18S rRNA gene was analyzed and the phylogeny tree was constructed based on Maximum Likelihood algorithm.【Result】The result showed that the infection rate in Northern Xinjiang was 13.96% (25/179), and the infection rate in Southern Xinjiang was 27.09% (78/299); It was thought that the strains in India, South Africa, Spain, Iran and other places clustered into one branch and the strains with the genes (MF398476, MF398477) and Swedish local strains clustered into another branch.【Conclusion】The infection rate of Equine Theileriosis between Northern and Southern Xinjiang displayed significant difference(P=0.001<0.05),but there was no significant difference(P>0.05)in different age groups. The amplified Theileria equi in Altay and Tuokexun endemic strains (MF398477, MF398476) and the local strains of Switzerland (KM046918.1) had the closest relationship. The test provided a data to support the control of Equine Theileriosis and the accurate classifying status of Theileria equi.

Key words: Theileria equi; northern and southern Xinjiang; phylogeny

中图分类号:

S852.7

刘世芳, 郭庆勇, 闻秀秀, 张梦园, 党娜娜, 阿拉西·阿滨, 巴音查汗. 中国新疆部分地区马泰勒虫感染的差异性及系统发育分析[J]. 新疆农业科学, 2017, 54(11): 2130-2137.

LIU Shi-fang, GUO Qing-yong, WEN Xiu-xiu, ZHANG Meng-yuan, DANG Na-na, Alax Abing, Bayinchahan. Differences in Theileria equi Infection and Its Phylogenetic Development Analysis in Some Areas of Xinjiang[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2130-2137.

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中国新疆部分地区马泰勒虫感染的差异性及系统发育分析

Differences in Theileria equi Infection and Its Phylogenetic Development Analysis in Some Areas of Xinjiang

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