新疆果园不同寄主腐烂病菌交互侵染研究

doi:10.6048/j.issn.1001-4330.2019.06.014

摘要/Abstract

摘要： 【目的】研究在新疆果园为微生态环境下不同寄主间腐烂病交互侵染情况。【方法】以新疆阿克苏地区五团中的苹果(M.pumila Mill)、新疆杨(Populus bolleana)和库尔勒香梨(Pyrus sinkiangensis)腐烂病病样为材料,采用枝条烫伤接种法进行交互侵染实验;利用β-tubulin和EF-1α基因序列,在NCBI数据库中查询同源基因的对应序列,再用邻结法(Neighbor-joining,NJ)构建系统发育树,进行序列分析。【结果】不同寄主来源的腐烂病菌在苹果、新疆杨和库尔勒香梨之间能够相互侵染发病。其中来源于香梨的腐烂病菌发病最早。其次为来源于新疆杨的腐烂病菌,来源于苹果腐烂病菌发病最晚。β-tubulin和EF-1α基因序列分析显示这三种不同寄主来源的腐烂病菌各自聚为一类。来源于苹果腐烂病菌的同源序列的寄主都为苹果。来源于新疆杨的同源序列的寄主有苹果和梨。来源于库尔勒香梨腐烂病菌的同源序列的寄主为杨树。【结论】新疆果园微生态环境下的苹果、香梨和新疆杨的腐烂病菌之间存在交互侵染现象。

关键词: 果园; 不同寄主腐烂病; 序列分析; 交互侵染

Abstract: 【Objective】 To find out whether there is a cross infection of rot pathogens in different hosts of orchard in Xinjiang in a micro-ecological environment. 【Method】Using the apple (M. pumila Mill), Xinjiang Populus bolleana and Pyrus sinkiangensis rot disease materials in the fifth Regimental Farm of Aksu prefecture of Xinjiang as the experimental material, the cross infection experiment was carried out by the shoot inoculation method; The tubulin and EF-1α gene sequences were constructed by Neighbor-joining (NJ) to analyze the phylogenetic tree. (M.pumila Mill)、(Populus bolleana)(Pyrus sinkiangensis)Using β-tubulin and EF-1 α gene sequences, the corresponding sequences of homologous genes were queried in NCBI database, and then the phylogenetic tree was constructed by neighbor-joining (NJ) method, and the sequence analysis was carried out. (Neighbor-joining,NJ)。【Result】The results of cross-infection showed that the rot pathogens of different host sources could infect each other between apple, Xinjiang poplar and Korla pear. Among them, the rot pathogens derived from pears were the earliest, followed by the rot pathogens originating from Xinjiang poplar; Those originated from apple rot pathogens had the latest onset. Sequence analysis of the β-tubulin and EF-1α genes revealed that the three different host-derived rot pathogens were grouped together. The host of the homologous sequence derived from the apple rot pathogen was apple. Hosts derived from the homologous sequences of Xinjiang poplar are apples and pears. The host of the homologous sequence derived from Korla pear rot pathogen was poplar. 【Conclusion】There existed am cross infection between apple, fragrant pear and poplar in Xinjiang orchard in microecological environment.

Key words: orchard ; different host rot disease; sequence analysis; cross infection

中图分类号:

S436.6

朱宗财, 张王斌, 李亚鹏, 严海璘, 仪子博. 新疆果园不同寄主腐烂病菌交互侵染研究[J]. 新疆农业科学, 2019, 56(6): 1112-1121.

ZHU Zong-cai, ZHANG Wang-bin, LI Ya-peng, YAN Hai-lin, YI Zi-bo. Study on the Cross Infection of Rot Pathogens in Different Hosts of Orchard in Xinjiang[J]. Xinjiang Agricultural Sciences, 2019, 56(6): 1112-1121.

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