盐角草PsaH基因的克隆及生物信息学分析

doi:10.6048/j.issn.1001-4330.2017.09.006

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (9): 1613-1620.DOI: 10.6048/j.issn.1001-4330.2017.09.006

盐角草PsaH基因的克隆及生物信息学分析

郝晓燕, 李建平, 足木热木·吐尔逊, 高升旗, 黄全生

新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091

收稿日期:2017-07-18 发布日期:2020-07-22
通信作者: 黄全生(1964-),男,新疆乌鲁木齐人,研究员,博士,博士生导师,研究方向为作物耐逆分子生物学,(E-mail)hquansheng@126.com
作者简介:郝晓燕(1980-),女,新疆人,副研究员,硕士,研究方向为植物抗逆分子生物学,(E-mail)hxy29@126.com
基金资助:
新疆维吾尔自治区自然科学基金项目“碱蓬SsPSI基因提高转基因烟草耐旱性的研究”(2015211A028);新疆农业科学院优秀青年科技人才基金“盐生植物盐角草耐盐基因的发掘与功能研究”(xjnky-2012-003)

Cloning and Bioinformatics Analysis of Salt-tolerance of PsaH Gene from Salicornia europaea

HAO Xiao-yan, LI Jian-ping, Zumuremu Turxun, GAO Sheng-qi, HUANG Quan-sheng

Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2017-07-18 Published:2020-07-22
Correspondence author: HUANG Quan-sheng(1964-),male, Urumqi, Xinjiang, Professor, supervisor, the main research directions for crop adversity molecular biology,(E-mail) hquansheng@126.com
Supported by:
Natural Science Foundation of Xinjiang "Study on Improving Drought Resistance of Transgenic Tobacco by Suaeda Salsa SsPSI Gene" (2015211A028) and Outstanding Young Scientific Talent Foundation of Xinjaing Academy of Agricultural Sciences "Discovery and Function of Salt Tolerant Genes in Halophytes" (xjnky-2012-003)

摘要/Abstract

摘要： 【目的】从盐生植物盐角草(Salicornia europaea)中克隆得到一个参与光合反应光系统I(PSI)复合蛋白H亚基的PsaH基因,分析该基因进行生物信息学,为研究该基因的作用奠定基础并为改良作物耐盐性分子育种提供候选基因。【方法】以盐生植物盐角草为实验材料,采用RT-PCR方法克隆PsaH基因,利用NCBI、MEGA、Expasy等生物信息学工具对其核酸序列、编码的氨基酸序列及蛋白质的结构和功能进行分析。【结果】克隆得到一个与耐盐有关的基因,命名为SePsaH,属于光系统I(PSI)家族H亚基成员,其开放阅读框(ORF)为438 bp,基因编码145个氨基酸,预测蛋白分子量为15.3 kD,等电点9.84,是亲水性蛋白;系统进化树分析表明其与菠菜亲缘关系较近,通过对该蛋白保守性分析发现其含有4个保守性结构域。【结论】获得盐角草SePsaH基因,为进一步研究该基因耐盐功能及其在盐生植物耐盐机制中的作用提供了基础。

关键词: 盐角草; SePsaH基因; 生物信息

Abstract: 【Objective】 To clone a novel PsaH gene from Salicornia europaea, and analyze its biological information for better understanding its role in function of salt-tolerance.【Method】Salicornia europaea was used as the plant material to clone the full-length cDNA sequence of SePsaH by RT-PCR. The encoding region and amino acid sequence of PsaH gene, and the structure and function of protein encoded by protein were analyzed by NCBI, MEGA and Expasy and other online Bioinformatics bioinformatics software for SePsaH gene.【Result】 Full-length cDNA sequence encoding photosystem I reaction center subunit H was cloned from Salicornia europaea and designated by the name of SePsaH, which was an opening reading frame of 438bp encoding 145 amino acids. The putative protein molecular weight was 15.3kD and its theoretical isoelectric points was 9.84, SePsaH was a hydrophilic protein; Phylogenetic analysis showed that SePsaH gene and Spinacia oleracea were closely related; Through the conservative analysis of the protein, we found that there were 4 conserved domain structures.【Conclusion】SePsaH gene was cloned, which has laid the foundation for further study on the gene function and the role in salt-tolerance of Salicornia europaea.

Key words: Salicornia europaea; photosystem I reaction center subunit H; bioinformatics

中图分类号:

S188

郝晓燕, 李建平, 足木热木·吐尔逊, 高升旗, 黄全生. 盐角草PsaH基因的克隆及生物信息学分析[J]. 新疆农业科学, 2017, 54(9): 1613-1620.

HAO Xiao-yan, LI Jian-ping, Zumuremu Turxun, GAO Sheng-qi, HUANG Quan-sheng. Cloning and Bioinformatics Analysis of Salt-tolerance of PsaH Gene from Salicornia europaea[J]. Xinjiang Agricultural Sciences, 2017, 54(9): 1613-1620.

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盐角草PsaH基因的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Salt-tolerance of PsaH Gene from Salicornia europaea

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