盐环境下氮素对苗期盐角草氮同化关键酶活性的影响

doi:10.6048/j.issn.1001-4330.2020.07.016

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (7): 1314-1320.DOI: 10.6048/j.issn.1001-4330.2020.07.016

盐环境下氮素对苗期盐角草氮同化关键酶活性的影响

黄建, 段婧婧, 马晓东, 祁通, 付彦博

新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830091

收稿日期:2020-02-28 出版日期:2020-07-20 发布日期:2020-06-22
通信作者: 付彦博(1986-),男,河南人,助理研究员,硕士,研究方向为土壤生态与农业节水,(E-mail)371293063@qq.com
作者简介:黄建(1986-),男,四川射洪人,助理研究员,硕士,研究方向为土壤生态与农业节水,(E-mail)287591792@qq.com
基金资助:
新疆维吾尔自治区自然科学基金"干旱区氮高效利用盐角草氮素吸收累积特性研究"(2016D01B039)

Effects of Nitrogen on the Activities of Key Enzymes for Nitrogen Assimilation of Salicornia europaea L. during Seedling Stage

HUANG Jian, DUAN Jingjing, MA Xiaodong, QI Tong, FU Yanbo

Research Institute of Soil, Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2020-02-28 Online:2020-07-20 Published:2020-06-22
Correspondence author: FU Yanbo(1986),male,(E-mail)371293063@qq.com

摘要/Abstract

摘要： 【目的】 研究盐环境下施氮水平对盐角草幼苗对氮催化和同化的能力,了解盐角草对氮素吸收转化的机制。【方法】 采用盆栽试验方法,研究不同施氮水平对盐环境下生长的盐角草幼苗氮同化关键酶活性的影响。【结果】 与不施氮处理相比,在重度盐环境条件下,施氮可现在提高盐角草幼苗根、茎、同化枝NR、GS、GOGAT、GLDH的活性;施N(1.2 g/kg)时,盐角草幼苗根、茎、同化枝活体NR活性、茎活体GS、GOGAT活性达到最大值;施N(2.4 g/kg)时,盐角草幼苗根、同化枝活体GS、GOGAT活性及根、茎、同化枝活体GLDH活性达到最大值。【结论】 盐环境下施氮能够显著增强盐角草幼苗氮同化关键酶活性,提高了盐角草对氮素胁迫能力,增强其对高氮环境的适应性。

关键词: 盐角草; 盐分; 氮素; 氮同化酶

Abstract: 【Objective】 In order to reveal the nitrogen catalysis and assimilation capacity of Salicornia europaea L. seedlings under nitrogen application in salt environment, and to deeply understand the mechanism of the grass to nitrogen uptake and transformation. 【Method】 The pot experiment method was used to study the effects of different nitrogen application levels on the key enzyme activities of nitrogen assimilation of Salicornia europaea L. seedlings growing under salt environment. 【Results】 The results showed that, compared with the treatment without nitrogen application, under severe salt environment conditions, nitrogen application increased the activities of roots, stems, assimilated shoots NR, GS, GOGAT, GLDH of the plant. Under N 1.2 g/kg, the NR activity, GS, GOGAT activity of the root, stem, and assimilated branches of Salicornia europaea L. seedlings reached their maximum values; When N (2.4 g / kg) was applied, the roots and assimilated branches of GS, GOGAT activity and GLDH activity of root, stem and assimilated branches reached the maximum. 【Conclusion】 Nitrogen application in salt environment can significantly enhance the key enzyme activities of nitrogen assimilation of Salicornia europaea L. seedlings, increase its ability to nitrogen stress, thus enhancing its adaptability to high nitrogen environment.

Key words: Salicornia europaea L.; salinity; nitrogen; nitrogen assimilation enzyme

中图分类号:

S188

黄建, 段婧婧, 马晓东, 祁通, 付彦博. 盐环境下氮素对苗期盐角草氮同化关键酶活性的影响[J]. 新疆农业科学, 2020, 57(7): 1314-1320.

HUANG Jian, DUAN Jingjing, MA Xiaodong, QI Tong, FU Yanbo. Effects of Nitrogen on the Activities of Key Enzymes for Nitrogen Assimilation of Salicornia europaea L. during Seedling Stage[J]. Xinjiang Agricultural Sciences, 2020, 57(7): 1314-1320.

参考文献

[1] Garnica M, Houdusse F, Zamarre A M, Garcia-Mina J M. Nitrate modifies the assimilation pattern of ammonium and urea in wheat seedlings [J]. Journal of the Science of Food and Agriculture, 2010, 90(3): 357-369.
[2] Du L F, Zhao T K, Zhang C J, An Z Z, Wu Q,Liu B C, Li P, Ma M T. Investigation on nitrate pollution in soils, ground water and vegetables ofthree typical farmlands in Beijing region [J]. Scientia Agricultura Sinica, 2009, 42(8): 2837-2843.
[3] Rieh T E, Ungar I A. Growth and Ion Accumulation in Salicornia Europaea under Saline Field Conditions [J]. Oecologia, 1982,54: 193–199.
[4] 王界平, 田长彦.氮肥对盐角草生长及矿质灰分累积的影响[J]. 干旱地区农业研究, 2011,1(29): 102–107.
WANG Jieping, TIAN Changyan. Effects of N fertilization on growth, mineral ash absorption and accumulation of Salicornia europaea L.[J]. Agricultural Research in The Arid Areas, 2011,1(29): 102–107.
[5] Dwyer D D, Wolde-Yohannis K. Germination, emergence, water use, and production of Russian thistle (Salsola kali L.)[J]. Agronomy Journal News Articles, 1972, 64(1): 52-55.
[6] 赵惠明. 盐生植物盐角草的资源特点及开发利用[J]. 科技通报, 2004, 20(2): 167–171.
ZHAO Huiming. Resourcecharacteristics and development of halophyte Salicornia [J]. Science Bulletin, 2004.20(2):102-107.
[7] Tikhomirova N A, Ushakova S A, Tikhomirov A A. Possibility of Salicornia europaea use for the human liquid wastes inclusion into BLSS intrasystemmass exchange [J]. Acta Astronautica,2008, 63: 1106–1110.
[8]Ushakova S A, Kovaleva N P, Tikhomirova N A, et al. Effect of Photosynthetically Active Radiation, Salinization, and Type of Nitrogen Nutrition on Growth of Salicornia europaea Plants[J]. Bioresource Technology, 2006, 53: 785–792.
[9] 汤玉玮, 林振武, 陈敬祥. 硝酸还原酶活力与作物耐肥性的相关及其在生化育种上应叫的探讨[J]. 中国农业科学, 1985,18(6):2055-2063.
TANG Yuwei, LIN Zhenwu, CHEN Jingxiang. Correlation between nitrate reductase activity and crop fertilizer tolerance and response to biochemical breeding [J].Scientia Agricultura Sinica, 1985,18(6):2055-2063.
[10] Li B Z, Mike M, Li S M, et al. Molecular basis and regulation of ammonium transporter in rice [J]. Rice Science, 2009, 16(4):314-322.
[11] 张华珍, 徐恒玉. 植物氮素同化过程中相关酶的研究进展[J]. 北方园艺, 2011,(20):180-183.
ZHANG Huazhen, XU Hengyu. Research progress on related enzymes in plant nitrogen assimilation [J]. Northern Horticulture, 2011,(20):180-183.
[12] 叶利庭, 朱文静, 吕华军, 等. 不同氮效率水稻生育后期氮素积累转运特征[J]. 土壤学报, 2010,47(2):303-310.
YE Liting, ZHU Wenjing, LÜ Huajun. Characteristics of nitrogen accumulation and translocation in late growth stage of rice with different nitrogen efficiency [J]. Bulletin of the Soil Science Society of China, 2010, 47(2):303-310.
[13] 陈胜勇, 李观康, 汪云, 等. 谷氨酰胺合成酶的研究进展[J]. 中国农学通报, 2010,26(22):45-49.
CHEN Shenyong, LI Guankang, WANG Yun. Research progress of glutamine synthetase [J]. Agricultural Science Bulletin, 2010, 26(22):45-49.
[14] 焦峰, 吴金花, 张兴梅, 等. 不同氮肥水平影响下的水稻根系吸氮率特性分析[J]. 土壤通报, 2009,40(6):1353-1355.
JIAO Feng, WU Jinhua, ZHANG Xingmei. Analysis of nitrogen uptake by rice roots under different nitrogen fertilizer levels [J]. Chinese Journal of Soil Science, 2009, 40(6):1353-1355.
[15] 张华珍, 徐恒玉. 植物氮素同化过程中相关酶的研究进展[J]. 北方园艺, 2011,(20):180-183.
ZHANG Huazhen, XU Hengyu. Research Progress on related enzymes in plant nitrogen assimilation [J]. Northern Horticulture, 2011, (20):180-183.
[16] 黄冰艳, 高伟, 苗利娟, 等. 谷氨酰胺合成酶基因研究进展及其在植物氮代谢调控中的应用[J]. 中国农学通报, 2010,26(23):53-57.
HUANG Bingyan, GAO Wei, MIAO Lijuan. Research progress of glutamine synthetase gene and its application in plant nitrogen metabolism regulation [J]. Chinese Agricultural Science Bulletin, 2010, 26(23):53-57.
[17] Sun H, Huang Q M, Su J. Highly effective expression of glutamine synthetase genes GS1 and GS2 in transgenic rice plants increase nitrogen-deficiency tolerance [J]. Journal of Plant Physiology and Molecular Biology, 2005, 31(5):492-498.

盐环境下氮素对苗期盐角草氮同化关键酶活性的影响

Effects of Nitrogen on the Activities of Key Enzymes for Nitrogen Assimilation of Salicornia europaea L. during Seedling Stage

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