Determination of StaphlococcusAureus in Food and Evaluation of the Fast Kit

doi:10.6048/j.issn.1001-4330.2022.02.018

Abstract

Abstract:

【Objective】 To analyze content of Staphylococcus aureus in food samples, and evaluate the accuracy and operability of the rapid kit for S.aureus. 【Methods】 Apple chips in bulk and fruit corn in vacuum packaging were sampled, and S.aureus was determined by the method in GB 4789,10-2006 and the rapid kit.S.aureus was taken as the target bacteria, artificial contamination treatments were used, and according to the Technical Specification for Evaluation of Rapid Food Testing Methods, the indexes of the rapid detection kit for S.aureus were evaluated. 【Results】 The highest content of S.aureus in samples of apple chips and fruit corn was 20 and 45 CFU/g, respectively, which were lower than the limit of pathogens in related foods.The sensitivity, specificity, false-negative rate, false-positive rate and relative accuracy of the kit were 93%, 100%, 6.6%, 0, and 96.7%, respectively.Significant difference was not found between the rapid kit and the reference method. 【Conclusion】 The rapid kit is easy to operate and detect S.aureus accurately, which is suitable for fast checks.

Key words: Staphylococcus aureus; rapid detection; evaluation

摘要：

【目的】 分析食品样品中金黄色葡萄球菌的含量,评估金黄色葡萄球菌快检试剂盒的可操作性和准确性。【方法】 分别购买市售苹果脆片和真空包装水果玉米,采用国标GB 4789.10-2006和快检试剂盒测定其金黄色葡萄球菌含量,并以金黄色葡萄球菌为目标菌,采用人工污染样品,根据《食品快速检测方法评价技术规范》评估其快检试剂盒的相关指标。【结果】 苹果脆片和水果玉米中金黄色葡萄球菌含量最高分别为20和50 CFU/g,均低于国标中相关食品中金黄色葡萄球菌限量要求。金黄色葡萄球菌快检试剂盒的灵敏度为93%,特异性为100%、假阴性率6.6%,假阳性率为0,显著性差异为0.5,相对准确度为96.7%,与参比方法检测数据差异不显著。【结论】 金黄色葡萄球菌快检试剂盒检验食品中的金黄色葡萄球菌操作简单,检验结果较为准确,适合基层开展金黄色葡萄球菌的快速筛查。

关键词: 金黄色葡萄球菌, 快速检测, 评估

CLC Number:

TAN Huilin, WU Zhonghong, JIN Yongseng, NIU Guiyang, BAO Dongdong, CHEN Haiyuan, ZHANG Zhidong. Determination of StaphlococcusAureus in Food and Evaluation of the Fast Kit[J]. Xinjiang Agricultural Sciences, 2022, 59(2): 410-416.

谭慧林, 吴忠红, 金永生, 牛贵洋, 包东东, 陈海元, 张志东. 食品中金黄色葡萄球菌的快速检测及其评估[J]. 新疆农业科学, 2022, 59(2): 410-416.

Figures/Tables 6

Table 1 The calculation of properties indexes of the rapid detection method

样品情况^a Sample	检测结果^bResult		总数 Total
样品情况^a Sample	阳性Positive	阴性Negative	总数 Total
阳性Positive	N₁₁	N₁₂	N₁.=N₁₁+N₁₂
阴性Negative	N₂₁	N₂₂	N₂.=N₂₁+N₂₂
总数Total	N.1=N₁₁+N₂₁	N.2=N₁₂+N₂₂	N=N₁.+N₂.
显著性差异( $χ^{2}$ )Significant difference( $χ^{2}$ )	$χ^{2}$ =(\|N₁₂-N₂₁\|-1)²/(N₁₂+N₂₁)		自由度(df)=1
灵敏度Sensitivity(p+,%)	p+=N₁₁/N₁.
特异性Specificity(p-,%)	p-=N₂₂/N₂.
假阴性率False negative rate(pf-,%	pf-=N₁₂/N₁.=100-灵敏度
假阳性率False negative rate(pf+,%)	pf+=N₂₁/N₂.=100-特异性
相对准确度Relative accuracy(%^c)	(N₁₁+N₂₂)/(N₁.+N₂.)

Table 1 The calculation of properties indexes of the rapid detection method

样品情况^a Sample	检测结果^bResult		总数 Total
样品情况^a Sample	阳性Positive	阴性Negative	总数 Total
阳性Positive	N₁₁	N₁₂	N₁.=N₁₁+N₁₂
阴性Negative	N₂₁	N₂₂	N₂.=N₂₁+N₂₂
总数Total	N.1=N₁₁+N₂₁	N.2=N₁₂+N₂₂	N=N₁.+N₂.
显著性差异( $χ^{2}$ )Significant difference( $χ^{2}$ )	$χ^{2}$ =(\|N₁₂-N₂₁\|-1)²/(N₁₂+N₂₁)		自由度(df)=1
灵敏度Sensitivity(p+,%)	p+=N₁₁/N₁.
特异性Specificity(p-,%)	p-=N₂₂/N₂.
假阴性率False negative rate(pf-,%	pf-=N₁₂/N₁.=100-灵敏度
假阳性率False negative rate(pf+,%)	pf+=N₂₁/N₂.=100-特异性
相对准确度Relative accuracy(%^c)	(N₁₁+N₂₂)/(N₁.+N₂.)

Table 2 Amount of S.aureus in the samplesby the standard method

样品 Sample	计数 Counting (CFU)				统计 Statistics (CFU/g)
样品 Sample	1∶10		1∶100		统计 Statistics (CFU/g)
苹果脆片 Apple chips	2	2	0	0	20
	2	2	0	0	20
	2	3	0	0	25
	3	2	0	0	25
	3	2	0	0	25
真空包装的水果玉米 Fruit corns in vacuum packaging	3	3	0	0	30
	4	3	0	0	35
	5	5	0	0	50
	5	4	0	0	45
	4	5	0	0	45

Table 3 Amount of S.aureus in the samples by the rapid kit

样品 Sample	计数 Counting (CFU)				统计 Statistics (CFU/g)
样品 Sample	1∶10		1∶100		统计 Statistics (CFU/g)
苹果脆片 Apple chips	3	2	0	0	25
	3	2	0	0	25
	2	2	0	0	20
	4	2	0	0	30
	2	2	0	0	20
真空包装水果玉米 Fruit corns in vacuum packaging	4	3	0	0	35
	4	4	0	0	40
	3	3	0	0	30
	3	3	0	0	30
	2	5	0	0	35

Table 4 Average recovery in different spiked levels for S.aureus by the standard method

样品 Sample	计数Accounting						统计 Statistics (CFU/g)	回收率 Rate of recovery (%)
样品 Sample	1∶10			1∶100			统计 Statistics (CFU/g)	回收率 Rate of recovery (%)
苹果脆片 (加标100 CFU/g) Apple chips (artificial pollution treatments, 100 CFU/g )	9	9	11	0	0	0	93	93.3
	8	10	9	0	0	0	93	93.3
苹果脆片 (加标1 000 CFU/g) Apple chips (artificial pollution treatments, 1,000 CFU/g )	97	96	108	10	7	8	1 053	102.5
	107	102	105	9	8	9	1 053	102.5

Table 5 Comparison of results detectedby the standard method and the rapid kit

	T₁	T₂	T₃	T₄	T₅
国标法检测结果 Results by the GB method (CFU/g)	26	86	123	862	1 053
测试片法检测结果 Results by the rapid kit method (CFU/g)	20	80	120	810	1 140
	20	90	110	910	1 180
	20	80	100	870	1 100
	30	80	110	860	1 020
	30	80	120	880	1 040
	20	90	120	910	1 030
	20	80	110	820	1 120
	20	90	110	840	1 030
	30	90	110	900	1 150
	30	80	100	810	1 010

Table 6 Estimation and analysis the fast kit for S.aureusdetermination

样品实际状态^a Sample	测试片检测结果^b Results		合计Total
样品实际状态^a Sample	阳性Postive	阴性 Negative	合计Total
阳性Postive(>100 CFU/g)	28	2	30
阴性Negative(≤100 CFU/g)	0	30	30
合计	30	30	60
评估指标Evaluation indexs
灵敏度 Sensitivity	特异性 Specificity	假阴性率 False negative rate	假阳性率 False negative rate
93%	100%	6.6%	0%

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