Effects of Different Concentrations of S-nitrosoglutathione (GSNO) on the Growth of Tomato Seedlings under Salt Stress

doi:10.6048/j.issn.1001-4330.2021.04.005

Abstract

Abstract: 【Objective】 To investigate the effects of different concentrations of S-nitrosoglutathione (GSNO) on the growth of tomato seedlings under salt stress in the hope of screening out the appropriate concentration to improve the salt tolerance of tomato. 【Methods】 The tomato variety Zhongshu 4 was used as the experimental material and nutrient solution cultivation method was used. Under the stress of NaCl (100 mmol/L), the leaves of tomato seedlings were sprayed with different concentrations of GSNO (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6 mmol/L). The effects of exogenous GSNO on the growth, root activity, chlorophyll content, malondialdehyde (MDA) content, electrolyte permeability and proline content of tomato seedlings under salt stress were determined. 【Results】 (1) NaCl stress significantly reduced plant height, stem diameter, dry weight, fresh weight, root activity, chlorophyll a, chlorophyll b and total chlorophyll content of tomato seedlings; significantly increased MDA content and proline content of tomato seedlings; had no significant effect on electrolyte permeability; (2) exogenous application of 0.1-0.3 mmol/L GSNO can effectively alleviate the inhibition of salt stress on Tomato Seedling Growth and improve salt adaptability by increasing chlorophyll content, root activity and proline content, reducing membrane lipid peroxidation and membrane integrity damage Although the application of GSNO did harm to the growth of Tomato Seedlings under salt stress, it did not inhibit the growth of Tomato Seedlings under salt stress. 【Conclusion】 NaCl stress significantly affected the normal growth of tomato seedlings, and exogenous GSNO with appropriate concentration could effectively alleviate the effect of salt stress on tomato seedlings. In this experiment, 0.1 mmol/L GSNO was the best.

Key words: tomato seedling; salt stress; S-nitrosoglutathione

摘要： 【目的】 研究不同浓度S-亚硝基谷胱甘肽(GSNO)对盐胁迫下番茄幼苗生长的影响,筛选出提高番茄耐盐性的适宜施用浓度。【方法】 选用番茄品种中蔬4号。采用营养液栽培法。NaCl(100 mmol/L)胁迫下,番茄幼苗叶面分别喷施不同浓度的GSNO(0、0.1、0.2、0.3、0.4、0.5、0.6 mmol/L)。测定分析不同浓度外源GSNO对盐胁迫下番茄幼苗生长及根系活力、叶绿素及丙二醛(MDA)含量、电解质渗透率和脯氨酸(Pro)含量的影响。【结果】 (1)NaCl胁迫显著降低了番茄幼苗的株高、茎粗、地上部和地下部干重、鲜重、根系活力、叶绿素a、叶绿素b和叶绿素总量;显著提高了番茄幼苗的丙二醛含量和脯氨酸含量;对电解质渗透率无显著影响;(2)外源施用0.1~0.3 mmol/L GSNO可通过不同程度地提高叶绿素含量、根系活力和脯氨酸含量,降低膜脂过氧化和膜完整性的破坏程度而有效缓解盐胁迫对番茄幼苗生长的抑制作用和提高盐适应性,而实验施用的高浓度(0.5~0.6 mmol/L)GSNO的施用虽对盐胁迫下番茄幼苗的已造成伤害,但尚未达到抑制盐胁迫下番茄幼苗生长的作用。【结论】 NaCl胁迫显著影响了番茄幼苗的正常生长,外源施加适宜浓度的GSNO能有效缓解盐胁迫对番茄幼苗的影响。以0.1 mmol/L GSNO施用效果最佳。

关键词: 番茄幼苗, 盐胁迫, S-亚硝基谷胱甘肽

CLC Number:

S641.3

SUN Yanyun, ZHU Pusheng, DIAO Ming, CUI Jinxia, XU Wei, LIU Huiying. Effects of Different Concentrations of S-nitrosoglutathione (GSNO) on the Growth of Tomato Seedlings under Salt Stress[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 625-633.

孙艳芸, 朱普生, 刁明, 崔金霞, 徐巍, 刘慧英. 不同浓度GSNO对盐胁迫下番茄幼苗生长的影响[J]. 新疆农业科学, 2021, 58(4): 625-633.

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