Effects of Temperature Stress on the Main Antioxidant Enzymes to Pupae in Carpomya vesuviana  Costa(Diptera: Tephritidae)

doi:10.6048/j.issn.1001-4330.2019.11.012

Abstract

Abstract: 【Objective】 To explore the adaptive mechanism of Carpomya vesuviana Costa with high temperature in Turpan.【Method】 In this project, the pupae of C. vesuviana were treated with temperature stress to measured the activities of Catalase (CAT), Peroxidase (POD), Superoxide dismutase (SOD) and Malondialdehyde (MDA) at different temperatures (45℃, 43℃, 41℃, 37℃, 33℃, 28℃ (CK), 4℃, and 0℃) for 3, 6 and 9 h, respectively, thus reflecting the changes of the antioxidant enzyme system.【Result】 The results showed that the MDA content in the pupae of C. vesuviana was significantly higher under high temperature and low temperature stress than that in CK (28℃), The MDA content was the highest at 33℃/6h, and the lowest MDA content was (0.039±0.012) nmol/(mg protein) at 0℃/9h. Besides, the activity of peroxidase (POD) was highest at 0 ℃/9h and the activity of catalase (CAT) was highest at 4℃/9h in C. vesuviana pupae after heat stress. The activity of superoxide dismutase (SOD) was the highest at 41 ℃ / 9 h, three peaks appeared at low temperature of 0 ℃, 4℃ and high temperature of 41℃. Therefore, the activity of antioxidant enzymes decreased significantly after the peaks of 0-4℃ in the low temperature zone and 41-45 ℃ in the high temperature zone. This decreasing trend was not significant at the treatment temperature of 33-37 ℃. The total antioxidant capacity (T-AOC) activity was highest at 41℃ / 6 h, and it could maintain a high activity value at higher or lower temperatures, and played a role of antioxidant stress stably. 【Conclusion 】 At extreme temperature, heat stress has an extremely significant effect on C. vesuviana, which reveals the effect of temperature stress on the antioxidant enzyme activity of C. vesuviana, and provides a scientific basis for explaining the heat tolerance of C. vesuviana from the physiological and biochemical perspective.

Key words: Carpomya vesuviana Costa; temperature; thermal stress; antioxidant enzyme

摘要： 【目的】研究枣实蝇(Carpomya vesuviana Costa)在吐鲁番高温的适应性机制,分析枣实蝇蛹逆境适应性,从生理生化角度解释枣实蝇蛹耐热性。【方法】通过温度胁迫处理枣实蝇的蛹,分别测定其体内的过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)以及丙二醛(MDA)等在45、43、41、37、33、28(CK)、4、0℃处理3、6和9 h下的活性,分析枣实蝇体内抗氧化物酶系统变化。【结果】高温和低温胁迫下枣实蝇蛹体内的MDA含量都较CK(28℃)显著升高,33℃/6 h时MDA含量最高,0℃/9 h时MDA含量最低(0.039±0.012) nmol/(mg protein);枣实蝇蛹遭受热胁迫后过氧化物酶(POD)活性在0℃/9 h下最高、过氧化氢酶(CAT)活性在4℃/9 h下最高,超氧化物歧化酶(SOD)活性在41℃/9 h下最高,在低温0、4℃和高温41℃下出现三个峰值;故抗氧化物酶活性在低温区0~4℃和高温区41~45℃的峰值后显著降低,此降低趋势在处理温度33~37℃下不显著;总抗氧化能力(T-AOC)活性在41℃/6 h下最高,且在较高或较低温度下均能保持较高的活性值,稳定地发挥着抗氧化胁迫的作用。【结论】在极端温度下,热胁迫对枣实蝇的蛹产生极显著的影响。

关键词: 枣实蝇, 温度, 热胁迫, 抗氧化物酶

CLC Number:

S436.65

LI Lan-jie, Adil Sattar. Effects of Temperature Stress on the Main Antioxidant Enzymes to Pupae in Carpomya vesuviana Costa(Diptera: Tephritidae)[J]. Xinjiang Agricultural Sciences, 2019, 56(11): 2062-2071.

李岚洁, 阿地力·沙塔尔. 温度胁迫对枣实蝇蛹体内主要抗氧化物酶的影响[J]. 新疆农业科学, 2019, 56(11): 2062-2071.

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Effects of Temperature Stress on the Main Antioxidant Enzymes to Pupae in Carpomya vesuviana Costa(Diptera: Tephritidae)

温度胁迫对枣实蝇蛹体内主要抗氧化物酶的影响

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