Occurrence and detection of begonia infected by apple stem pitting virus

doi:10.6048/j.issn.1001-4330.2024.12.021

Abstract

Abstract:

【Objective】 To identify the species and field infection of viral diseases on ornamental Malus spp.【Methods】 A total of 158 samples from 7 areas of Xinjiang were detected by macro virus sequencing technique combined with RT-PCR.【Results】 Only one plant virus was compared with apple stem pitting virus (ASPV).Among 158 samples of ornamental begonia, the ASPV detection rates were 0.00%, 12.00%, 0.00%, 31.71%, 29.41%, 17.65% and 0.00% in Bayingoleng Mongolian Autonomous Prefecture, Aksu Region, Kashgar Region, Changji Hui Autonomous Prefecture, Shihezi City, Yili Kazak Autonomous Prefecture and Kuitun City, respectively.Among the 7 areas tested, Changji had the highest detection rate (31.71%).The detection rate of Shihezi City was 29.41%.ASPV was not detected in Kashgar Prefecture and Kuitun City.【Conclusion】 The results showed that the detection rate of ASPV on ornamental begonia is higher in northern Xinjiang and lower in southern Xinjiang.ASPV is detected in ornamental begonia samples, and the virus detected samples were all symptomatic leaves in the field, which was consistent with the disease observed in the field.Ornamental begonia is one of the natural hosts of ASPV.

Key words: ornamental begonia; macro virus sequencing; apple stem pitting virus; RT-PCR; virus detection

摘要：

【目的】 研究海棠(Malus spp.)上病毒病的种类及田间侵染情况。【方法】 利用宏病毒组测序技术结合RT-PCR方法,对采自新疆7个海棠种植区的158份样品进行病毒检测。【结果】 宏病毒组测序结果只对比到苹果茎痘病毒(Apple stem pitting virus, ASPV)一种植物病毒。158份海棠样品中巴音郭楞蒙古自治州(简称巴州)、阿克苏地区、喀什地区、昌吉回族自治州(简称昌吉州)、石河子市、伊犁哈萨克自治州(简称伊犁州)、奎屯市ASPV检出率分别为0.00%、12.00%、0.00%、31.71%、29.41%、17.65%和0.00%。在检测的7个种植区中,昌吉州检出率最高,为31.71%;石河子市检出率次之,为29.41%;巴州、喀什地区和奎屯市未检测到ASPV。【结论】 海棠上的ASPV在北疆检出率较高,在南疆检出率较低。在观赏海棠样品中检测到ASPV,其病毒检出样本均为田间发病症状叶片,与田间观察到的发病情况相符合,海棠为ASPV的自然寄主之一。

关键词: 海棠, 宏病毒组测序, 苹果茎痘病毒, RT-PCR, 病毒检测

CLC Number:

S436

ZHANG Liya, LIU Baojun, SHAN Jiaqi, WANG Shu, GU Aixing, BAI Jianyu. Occurrence and detection of begonia infected by apple stem pitting virus[J]. Xinjiang Agricultural Sciences, 2024, 61(12): 3061-3066.

张李娅, 刘保军, 单佳祁, 王澍, 顾爱星, 白剑宇. 海棠感染苹果茎痘病毒发生与检测[J]. 新疆农业科学, 2024, 61(12): 3061-3066.

Figures/Tables 5

Fig.1 The Leaves of ornamental begonia phenotypes of healthy and suspected viral disease Note: A: Healthy leaves; B, C, D: suspected susceptible leaves

Fig.2 Detection of apple stem pitting virus specific primers by PCR Note: M: DL2000 DNA Marker; 1: Apple stem pox virus; CK: Purified water

Fig.3 Cloning positive identification PCR detection Note:M: DL5000 DNA Marker; 1-8: Clone positive identification of bacterial samples

Fig.4 Results of PCR detection of apple stem poxvirus in some samples Note: M: DL2000 DNA Marker; 1-16: Test sample; CK: Purified water

Tab.1 Field test results

采样地点 Sampling site	采集样品数 Sample collection	检出样品数 Number of samples detected	检出率 Detection rate(%)
库尔勒 Korla	15	0	0.00
阿克苏 Aksu	25	3	12.00
喀什 Kashgar	7	0	0.00
昌吉 Changji	41	13	31.71
石河子 Shihezi	17	5	29.41
伊犁 Ili	34	6	17.65
奎屯 Kuytun	19	0	0.00
总计 Total	158	27	17.09

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