Optimization of high-efficiency decomposition conditions and transformation parameters of grapevine branches eaten by the Protaetia brevitarsis Lewis

doi:10.6048/j.issn.1001-4330.2024.12.022

Abstract

Abstract:

【Objective】 Optimization of high-efficiency decomposition conditions and transformation parameters of grapevine branches eaten by the Protaetia brevitarsis Lewis,which has provided a new way for the resource utilization of grape branches.【Methods】 Crushed grape branches were taken as the main raw materials, the orthogonal experiment of 3 factors and 5 levels was performed, which included the decomposition inoculants, the ratio of cow dung (%) and the number of fermentation days (d).By measuring the conversion power of larvae to the materials, the optimal combination of feeding grape branches by the larva was screened and verified.【Results】 The results showed that the proportion of cow dung reached a significant difference level at the 10 th, 15 th, 25 th and 30 th days.The higher the proportion of cow dung, the higher the temperature, indicating the better fermentation rate, and the addition of decomposition inoculants and cow dung had a better promotion effect on the fermentation of materials.After fermentation for 25 days, 96 g of compound feed was obtained from each 120 g of raw grape branches, supplemented with 80g of cow dung and 1‰ VT decomposition inoculants.After the conversion of the 3rd larva, the insect sand content was (77.53±22.43)g, the insect body conversion rate was (37.16%±0.04%), and the insect sand conversion rate was (84.99%±0.01%).The harvest coefficient of insect sand was (59.47%±0.04%), and the utilization rate of organic materials was (61.17%±0.02%).【Conclusion】 The 3rd instar larvae of the Protaetia brevitars has a good conversion ability to ferment grape branch feed.Adding 1%VT decomposition inoculants, 40% cow dung and after 25 d fermentation is the best fermentation scheme.

Key words: grape branches; larvae of Protaetia brevitarsis Lewis; decomposing inoculants; biotransformation; resource utilization

摘要：

【目的】 研究白星花金龟取食葡萄枝条的高效腐解条件和转化参数优化,为葡萄枝条的资源化利用提供新路径。【方法】 以粉碎的葡萄枝条为主要原料,开展腐解菌剂、牛粪配比(%)和酵化天数(d)的3因素5水平正交试验,测定幼虫对物料的转化力,筛选并验证白星花金龟幼虫取食葡萄枝条的最优组合。【结果】 牛粪配比在第10、15、25和30 d达到显著差异水平,牛粪配比越高,其温度越高,酵化速度越好,添加菌剂和牛粪对物料的酵化有较好的促进作用;每120 g的葡萄枝条原料,辅加80 g的牛粪,添加1‰VT菌剂,发酵25 d后,获得配合饲料96 g,经白星花金龟3龄幼虫转化后,虫砂量为(77.53±22.43) g,虫体转化率(37.16%±0.04%),虫砂转化率为(84.99%±0.01%),虫砂收获系数(59.47%±0.04%),有机物料利用率在(61.17%±0.02%)。【结论】 白星花金龟3龄幼虫对发酵葡萄枝条饲料具有较好的转化力,添加1%VT菌剂、40%牛粪,酵化25 d为最佳酵化方案。

关键词: 葡萄枝条, 白星花金龟幼虫, 腐解菌剂, 生物转化, 资源化利用

CLC Number:

S432

XU Andong, ZHANG Guangjie, FU Rao, MENG Zhuo, YAN Yu, LI Baoguo, MA Deying. Optimization of high-efficiency decomposition conditions and transformation parameters of grapevine branches eaten by the Protaetia brevitarsis Lewis[J]. Xinjiang Agricultural Sciences, 2024, 61(12): 3067-3077.

徐安东, 张广杰, 付娆, 孟卓, 颜宇, 李保国, 马德英. 白星花金龟取食葡萄枝条的高效腐解条件和转化参数优化[J]. 新疆农业科学, 2024, 61(12): 3067-3077.

Figures/Tables 16

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腐解菌剂 Decomposition inoculant	主要功能菌 Main functional bacteria	有效活菌数 Effective number ofviable bacteria (100 million/g)	推荐用量 Recomm ended dosage (kg/t)
RW特配型 (鹤壁人元)	细菌(枯草、地衣、胶冻)、丝状菌和酵母菌	100	10
VT-1000 (北京沃土)	芽孢、放线、乳酸和霉菌	200	1
LK (中农绿康)	芽孢、木霉和酵母	8	0.5
LL(山东绿陇)	枯草/地衣芽孢杆菌, 酵母、绿色木霉	200	10
EM(日本)	酵母菌、乳酸菌	100	10

腐解菌剂 Decomposition inoculant	主要功能菌 Main functional bacteria	有效活菌数 Effective number ofviable bacteria (100 million/g)	推荐用量 Recomm ended dosage (kg/t)
RW特配型 (鹤壁人元)	细菌(枯草、地衣、胶冻)、丝状菌和酵母菌	100	10
VT-1000 (北京沃土)	芽孢、放线、乳酸和霉菌	200	1
LK (中农绿康)	芽孢、木霉和酵母	8	0.5
LL(山东绿陇)	枯草/地衣芽孢杆菌, 酵母、绿色木霉	200	10
EM(日本)	酵母菌、乳酸菌	100	10

水平 Levels	因素Factors
水平 Levels	腐解菌剂 Decomposition inoculants	牛粪配比 Manure proportion (%)	酵化天数 Fermentation days (d)
1	RW	10	10
2	VT	20	15
3	LK	30	20
4	LL	40	25
5	EM	50	30

水平 Levels	因素Factors
水平 Levels	腐解菌剂 Decomposition inoculants	牛粪配比 Manure proportion (%)	酵化天数 Fermentation days (d)
1	RW	10	10
2	VT	20	15
3	LK	30	20
4	LL	40	25
5	EM	50	30

处理 Treatments	因素Factors
处理 Treatments	腐解菌剂 Decomposition inoculants	牛粪配比 Manure proportion (%)	酵化天数 Fermentation days (d)
CK	0	0	0
CK₁	0	0	10
CK₂	0	0	15
CK₃	0	0	20
CK₄	0	0	25
CK₅	0	0	30