Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (10): 2551-2557.DOI: 10.6048/j.issn.1001-4330.2023.10.025
• Plant Protection·Microbes·Soil Fertilizer • Previous Articles Next Articles
LI Feng1(), LAI Ganggang1,2, ZHAO Zhihui1, CHEN Xiaofei1, ZHU Tiansheng1(
)
Received:
2023-01-13
Online:
2023-10-20
Published:
2023-11-01
Correspondence author:
ZHU Tiansheng (1974-),male, native place: Pingliang, Gansu Province. professor, research field: plant pathology (E-mail)Supported by:
李丰1(), 赖刚刚1,2, 赵志惠1, 陈小飞1, 朱天生1(
)
通讯作者:
朱天生(1974-),男,甘肃平凉人,教授,硕士生导师,研究方向为植物病理,(E-mail)作者简介:
李丰(1998-),男,甘肃定西人,硕士研究生,研究方向为植物病理,(E-mail)2925008071@qq.com
基金资助:
CLC Number:
LI Feng, LAI Ganggang, ZHAO Zhihui, CHEN Xiaofei, ZHU Tiansheng. Identification and 16S rRNA gene diversity analysis of salt cedar(Tamarix chinensis)witches'-broom[J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2551-2557.
李丰, 赖刚刚, 赵志惠, 陈小飞, 朱天生. 柽柳丛枝植原体鉴定及16S rRNA基因多样性分析[J]. 新疆农业科学, 2023, 60(10): 2551-2557.
等级 Grade | 分级标准 Standard | 柽柳感病情况 Susceptibility of tamarix chinensis |
---|---|---|
0 | 健康 | 未发病 |
I | 轻度 | 开始发病,不显著 |
Ⅱ | 中度 | 病枝数量达总枝数量的1/3, 其余枝条正常 |
Ⅲ | 较严重 | 病枝数量达总枝数量的2/3, 尚有正常枝条 |
IV | 严重 | 病枝数量达总枝数量的2/3以上, 正常枝条较少 |
Tab.1 Grading standard of disease index of SCWB disease
等级 Grade | 分级标准 Standard | 柽柳感病情况 Susceptibility of tamarix chinensis |
---|---|---|
0 | 健康 | 未发病 |
I | 轻度 | 开始发病,不显著 |
Ⅱ | 中度 | 病枝数量达总枝数量的1/3, 其余枝条正常 |
Ⅲ | 较严重 | 病枝数量达总枝数量的2/3, 尚有正常枝条 |
IV | 严重 | 病枝数量达总枝数量的2/3以上, 正常枝条较少 |
调查地区 Survey area | 调查总数 Total number of investigations | 发病总数 Total number of cases | 平均 发病率 Average incidence (%) | 平均病 情指数 Mean disease index |
---|---|---|---|---|
温宿县 Wensu | 116 | 22 | 19.08a | 3.45a |
阿拉尔市12团 Aral city 12th regiment | 390 | 72 | 18.20ab | 8.25b |
图木舒克市 Tumxuk | 153 | 24 | 15.71bc | 5.47b |
巴楚县 Bachu | 294 | 47 | 15.54bc | 3.18b |
阿拉尔市 Aral | 271 | 36 | 13.40d | 4.172c |
伽师县 Jiashi | 179 | 20 | 11.18d | 3.69c |
阿克苏市 Akesu | 206 | 14 | 6.60e | 7.56c |
昆玉市 Kunyu | 267 | 0 | 0.00f | 0.00d |
和田地区 Hetian | 858 | 235 | 0.00f | 0.00d |
平均 Average | 9.06 | 5.66 |
Tab.2 Incidence and disease index of SCWB disease in different areas
调查地区 Survey area | 调查总数 Total number of investigations | 发病总数 Total number of cases | 平均 发病率 Average incidence (%) | 平均病 情指数 Mean disease index |
---|---|---|---|---|
温宿县 Wensu | 116 | 22 | 19.08a | 3.45a |
阿拉尔市12团 Aral city 12th regiment | 390 | 72 | 18.20ab | 8.25b |
图木舒克市 Tumxuk | 153 | 24 | 15.71bc | 5.47b |
巴楚县 Bachu | 294 | 47 | 15.54bc | 3.18b |
阿拉尔市 Aral | 271 | 36 | 13.40d | 4.172c |
伽师县 Jiashi | 179 | 20 | 11.18d | 3.69c |
阿克苏市 Akesu | 206 | 14 | 6.60e | 7.56c |
昆玉市 Kunyu | 267 | 0 | 0.00f | 0.00d |
和田地区 Hetian | 858 | 235 | 0.00f | 0.00d |
平均 Average | 9.06 | 5.66 |
登录号 Login Number | OL687359 | OL687355 | OL687360 | OL687364 | OL687373 | OL687370 | OL687381 |
---|---|---|---|---|---|---|---|
OL687359 | 100.0 | 2.0 | 2.1 | 2.4 | 2.0 | 2.1 | 3.6 |
OL687355 | 98.0 | 100.0 | 0.4 | 1.8 | 0.4 | 0.3 | 2.6 |
OL687360 | 97.9 | 99.6 | 100.0 | 1.7 | 0.3 | 0.2 | 2.7 |
OL687364 | 97.7 | 98.2 | 98.3 | 100.0 | 1.6 | 1.7 | 3.3 |
OL687373 | 98.0 | 99.6 | 99.7 | 98.4 | 100.0 | 0.3 | 2.7 |
OL687370 | 97.9 | 99.7 | 99.8 | 98.3 | 99.8 | 100.0 | 2.5 |
OL687381 | 96.5 | 97.5 | 97.3 | 96.8 | 97.4 | 97.6 | 100.0 |
Tab.3 Nucleotide homology of 16S rRNA gene of SCWB phytoplasma in different regions
登录号 Login Number | OL687359 | OL687355 | OL687360 | OL687364 | OL687373 | OL687370 | OL687381 |
---|---|---|---|---|---|---|---|
OL687359 | 100.0 | 2.0 | 2.1 | 2.4 | 2.0 | 2.1 | 3.6 |
OL687355 | 98.0 | 100.0 | 0.4 | 1.8 | 0.4 | 0.3 | 2.6 |
OL687360 | 97.9 | 99.6 | 100.0 | 1.7 | 0.3 | 0.2 | 2.7 |
OL687364 | 97.7 | 98.2 | 98.3 | 100.0 | 1.6 | 1.7 | 3.3 |
OL687373 | 98.0 | 99.6 | 99.7 | 98.4 | 100.0 | 0.3 | 2.7 |
OL687370 | 97.9 | 99.7 | 99.8 | 98.3 | 99.8 | 100.0 | 2.5 |
OL687381 | 96.5 | 97.5 | 97.3 | 96.8 | 97.4 | 97.6 | 100.0 |
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