Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (1): 253-259.DOI: 10.6048/j.issn.1001-4330.2024.01.027
• Agricultural Equipment Engineering and Mechanization·Animal Husbandry Veterinarian • Previous Articles
MA Xiaojing(), GU Wenxi, YE Feng, LIU Shuai, LIU Liya, XIE Caiyun, ZHONG Qi, YI Xinping(
)
Received:
2023-05-11
Online:
2024-01-20
Published:
2024-02-21
Correspondence author:
YI Xinping(1970-),female,Hubei province,Researcher,Doctor,research field:Diagnose and prevention technology of Animal infectious diseases,(E-mail)Supported by:
马晓菁(), 谷文喜, 叶锋, 刘帅, 刘丽娅, 谢彩云, 钟旗, 易新萍(
)
通讯作者:
易新萍(1970-),女,湖北公安人,研究员,博士,研究方向为动物传染病诊断与防控,(E-mail)作者简介:
马晓菁(1983-),女,河南荥阳人,高级实验师,硕士,研究方向为布鲁氏菌病分子生物学诊断,(E-mail)mxj20030520@163.com
基金资助:
CLC Number:
MA Xiaojing, GU Wenxi, YE Feng, LIU Shuai, LIU Liya, XIE Caiyun, ZHONG Qi, YI Xinping. Sequence analysis and one-step RT-PCR establishment of VP7 protein coding gene of bluetongue isolated in Xinjiang[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 253-259.
马晓菁, 谷文喜, 叶锋, 刘帅, 刘丽娅, 谢彩云, 钟旗, 易新萍. 蓝舌病新疆分离株VP7蛋白编码基因序列分析及一步法RT-PCR方法的建立[J]. 新疆农业科学, 2024, 61(1): 253-259.
引物名称 Primers | 目的基因 Target gene | 序列(5’-3’) Sequence(5’-3’) | 片段大小(bp) Fragment size(bp) |
---|---|---|---|
F-7X | BTV S7 | CAGCAAGGGCGCTCACTGTGATG | 1010 |
F-7R | GCGTGTGAGCGGGCCCGGCATAGCATTCACA |
Tab.1 One step RT-PCR amplification of primers
引物名称 Primers | 目的基因 Target gene | 序列(5’-3’) Sequence(5’-3’) | 片段大小(bp) Fragment size(bp) |
---|---|---|---|
F-7X | BTV S7 | CAGCAAGGGCGCTCACTGTGATG | 1010 |
F-7R | GCGTGTGAGCGGGCCCGGCATAGCATTCACA |
Fig.1 The results of nested PCR amplification of BTV Xinjiang isolates Note:M:DNAMarkerDM2000;1-2:RNA extraction from BHK cells;3-4:RNA extraction from BTV Xinjiang isolate
Fig.2 One-step RT-PCR amplification results of BTV Xinjiang isolate Note:M:DNAMarkerDL2000; 1:RNA extraction from BHK cells;2:ddH20 blank control; 3:RNAextraction from Bovine Viral Diarrhoes Mucosal Virus;4:DNA extraction from Infectious Bovine Rhinotracheitis Virus;5:RNA extraction from BTV Xinjiang isolate
Fig.3 Sensitivity results of one-step RT-PCR for BTV Xinjiang isolate Note:M:DNAMarkerDL2000;1:RNA extraction from BHK cells;2:Standard plasmids 4×106copies/μL;3:Standard plasmids 4×105copies/μL;4:Standard plasmids 4×104copies/μL;5:Standard plasmids 4×103copies/μL;6:Standard plasmids 4×102copies/μL
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