Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (6): 1527-1534.DOI: 10.6048/j.issn.1001-4330.2024.06.027

• Microbes • Prataculture • Previous Articles     Next Articles

Extraction and decolorization of polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang

Nuerziya Yalimaimaiti1(), LUO Ying1, JIA Peisong1, HAO Jingzhe1(), JIA Wenjie1(), ZHU Qi1, LIANG Zhihao2, LIN Siru2   

  1. 1. Key Laboratory of Integrated Crop Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs/Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
    2. College of Agricalture Xinjiang Agriculture University, Urumqi 830052, China
  • Received:2023-12-22 Online:2024-06-20 Published:2024-08-08
  • Correspondence author: HAO Jingzhe, JIA Wenjie
  • Supported by:
    National Natural Science Foundation Projects(32060709);National Natural Science Foundation Projects(31560577);Earmarked Fund for CARS(CARS-20)

新疆野生巴尔喀什蘑菇子实体多糖的提取与脱色工艺

努尔孜亚·亚力买买提1(), 罗影1, 贾培松1, 郝敬喆1(), 贾文捷1(), 朱琦1, 梁志豪2, 蔺思茹2, 赵振蒙2   

  1. 1.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091
    2.新疆农业大学农学院,乌鲁木齐 830091
  • 通讯作者: 郝敬喆,贾文捷
  • 作者简介:努尔孜亚·亚力买买提(1977-),女,新疆伊宁人,副研究员,研究方向为食用菌驯化与栽培, (E-mail)nrzy729@163.com
  • 基金资助:
    国家自然科学基金地区基金项目(32060709);国家自然科学基金地区基金项目(31560577);现代农业产业技术体系建设专项资金(CARS-20)

Abstract:

【Objective】 To study the extraction and decolorization process of crude polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang, and provide theoretical basis and technical support for its in-depth development. 【Methods】 This study adopted ethanol subsiding to extract the fruit body polysaccharides of wild A. balchaschensisin, and explored the influence of extraction temperature, time and frequency on the yield of polysaccharides from A. balchaschensisin. With the decolorization time, decolorization temperature, content of activated carbon and H2O2 as influencing factors and the decolorization rate of polysaccharides as research index, the study optimized the decolorization process of polysaccharides from A. balchaschensisin through orthogonal test, so as to determine the optimal decolorization conditions. 【Results】 According to the results, the optimal conditions for extracting fruit body polysaccharides of wild A. balchaschensis by ethanol subsiding were 6 h extraction time, 60℃ extraction temperature, and 2 times extraction, under which the yield of fruit body polysaccharides of wild A.balchaschensisin was 15.6%. The optimal decolorization process for activated carbon was 25% activated carbon, 60℃ decolorization temperature, and 45 min decolorization time at pH3, under which the decolorization rate was 80.4%. The optimal decolorization process for hydrogen peroxide was 10% H2O2 content, 60℃ decolorization temperature, and 3 h decolorization time at pH 9, under which the decolorization rate was 86.23%. 【Conclusion】 This technology has verified the feasibility and practical value of applying ethanol subsiding to extract fruit body polysaccharides of wild A.balchaschensis in and the effects of H2O2 and activated carbon decolorization methods on the decolorization of polysaccharides from A. balchaschensis varied greatly under different process conditions. The former has a higher decolorization rate than the latter and is feasible for the decolorization of polysaccharides from A.balchaschensisin.

Key words: Agaricus balchaschensis; carbon; hydrogen peroxide (H2O2 ) decolorization

摘要:

【目的】研究新疆野生巴尔喀什蘑菇子实体粗多糖提取及多糖脱色工艺,为新疆野生巴尔喀什蘑菇多糖的纯化及深度开发提供理论依据和技术支持。【方法】釆用热水提醇沉法提取巴尔喀什蘑菇子实体粗多糖,研究提取温度、时间、次数对巴尔喀什蘑菇多糖得率的影响;选择脱色时间、脱色温度、活性炭和H2O2含量为影响因子,以多糖脱色率为研究指标,采用正交试验优化巴尔喀什蘑菇多糖脱色工艺,确定最佳脱色工艺条件。【结果】热水提醇沉法提取巴尔喀什菇子实体粗多糖的最佳工艺条件:提取时间6 h、提取温度60℃、提取次数2次,在此条件下巴尔喀什菇子实体粗多糖得率为15.6%。活性炭最佳脱色工艺为活性炭用量 25%、脱色温度 60℃、脱色时间45 min、pH值 3,在此条件下脱色率 为80.4%;双氧水最佳脱色工艺为H2O2含量10%、脱色温度60℃、脱色时间3 h、pH值9,在此条件下脱色率为86.23%。【结论】利用热水提醇沉法提取巴尔喀什蘑菇粗多糖工艺具有实际应用价值。H2O2脱色法和活性炭脱色法对巴尔喀什蘑菇多糖的脱色效果受不同工艺条件的影响差异较大,H2O2脱色率比活性炭脱色率高,是巴尔喀什蘑菇多糖脱色的可行方法。

关键词: 巴尔喀什蘑菇, 多糖提取, 活性炭脱色, 双氧水(H2O2)脱色

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