Correlation analysis of lignin degradation and enzyme activity changes in solid fermentation of cotton stalks

doi:10.6048/j.issn.1001-4330.2023.06.015

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (6): 1423-1432.DOI: 10.6048/j.issn.1001-4330.2023.06.015

• Microbes·Horticultural Special Local Products·Storage and Preservation Processing • Previous Articles Next Articles

Correlation analysis of lignin degradation and enzyme activity changes in solid fermentation of cotton stalks

LI Peiqi¹(), SUN Qingpei¹, WANG Zhihui², QIN Xinzheng²(), FAN Yonghong¹()

1. College of Life Science and Technology, Xinjiang University / Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China
2. Xinjiang Special Environmental Microbiology Laboratory Research/ Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2022-09-29 Online:2023-06-20 Published:2023-06-20
Correspondence author: QIN Xinzheng(1977-), male, native place: Shihezi, Xinjiang. Associate researcher. Research field: Environmental microbiology, (E-mail) qinxinzheng@163.com; FAN Yonghong(1974-), female, native place: Jinning, Gansu. Professor. Research field: Environmental microbiology, (E-mail) yhfanzyb2004@163.com
Supported by:
Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China(KY2020106);The Natural Science Foundation of Xinjiang Uygur Autonomous Region(2017D01A49)

棉秆固体发酵中木质素降解与酶活性变化的关联分析

李佩琪¹(), 孙庆培¹, 王志慧², 秦新政²(), 樊永红¹()

1.新疆大学生命科学与技术学院/新疆生物资源基因工程重点实验室,乌鲁木齐 830046
2.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091

通讯作者: 秦新政(1977-),新疆石河子人,副研究员,硕士,研究方向为环境微生物学,(E-mail) qinxinzheng@163.com; 樊永红(1974-),女,甘肃静宁人,教授,博士,硕士生导师,研究方向为环境微生物学,(E-mail) yhfanzyb2004@163.com
作者简介:李佩琪(1997-),女,硕士研究生,研究方向为环境微生物学,(E-mail)2394767902@qq.com
基金资助:
新疆维吾尔自治区公益性科研院所基本科研业务经费项目(KY2020106);新疆维吾尔自治区自然科学基金项目(2017D01A49)

Abstract

Abstract:

【Objective】 To elaborate on the correlation between lignin degradation and lignin degradation enzyme activity in the process of solid fermentation into organic matrix of cotton straw.【Methods】 The solid fermentation of cotton stalks was performed and meanwhile, the temperature, cellulose and hemicellulose content were determined. After that, the content changes of acid-soluble and acid-insoluble lignin and the activities of three main lignin-degrading enzymes during fermentation were determined by UV detection and high-temperature burning. 【Results】 The results showed that during the solid fermentation of cotton stalks, the lignin content of cotton stalks was significantly reduced from 31.51%to 25.38% (P<0.05), and the lignin degradation rate increased significantly after the high temperature stage, continued to increase at the curing stage, and reached the highest value at the end of fermentation, which was 13.84 g/d. The activities of the three enzymes continued to increase at the ripening stage, and reached the highest value at the end of fermentation, in which the manganese peroxidase activity was 12.91 U/g, lignin peroxidase activity was 55.28 U/g, and laccase activity was 0.19 U/g. Pearson correlation analysis showed that there was a significant positive correlation between lignin degradation enzyme activity and lignin degradation rate (P<0.01), and there was also a significant positive correlation between the three lignin degradation enzyme activities (P<0.01); principal component analysis showed that manganese peroxidase played a major role in the degradation of lignin in the solid fermentation of cotton stalks. 【Conclusion】 The lignin degradation and its relationship with lignin degradation enzyme activity in solid fermentation process of cotton stalk are related, which has laid a foundation for further research on the mechanism of lignin degradation.

Key words: cotton stalks; solid fermentation; lignin degradation; lignin-degrading enzyme; correlation

摘要：

【目的】分析棉花秸秆经固体发酵转化为有机基质过程中的木质素降解与木质素降解酶活性的关联性,为研究木质素降解机理奠定基础。【方法】对棉秆进行固体发酵,测定温度、纤维素及半纤维素含量,利用紫外检测和高温灼烧法分别测定发酵过程中酸溶性木质素和酸不溶性木质素的含量变化以及3种主要的木质素降解酶的活性。【结果】在棉秆固体发酵过程中,棉秆木质素含量由31.51%显著降低至25.38%(P<0.05),木质素降解速率在高温期后显著升高,在熟化期时持续升高,直至发酵结束时达到最高,为13.84 g/d。木质素降解酶活性在熟化期时显著升高,在发酵结束时达到最高值,其中锰过氧化物酶活性12.91 U/g,木质素过氧化物酶活性55.28 U/g,漆酶活性0.19 U/g。木质素降解酶活性和木质素降解速率呈显著正相关(P<0.01),3种木质素降解酶活性之间也呈显著正相关(P<0.01);锰过氧化物酶在棉秆固体发酵的木质素降解中起到主要作用。【结论】棉秆固体发酵过程中木质素降解的规律及其与木质素降解酶活性的关联。

关键词: 棉秆, 固体发酵, 木质素降解, 木质素降解酶, 相关性

CLC Number:

S435.112.6

LI Peiqi, SUN Qingpei, WANG Zhihui, QIN Xinzheng, FAN Yonghong. Correlation analysis of lignin degradation and enzyme activity changes in solid fermentation of cotton stalks[J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1423-1432.

李佩琪, 孙庆培, 王志慧, 秦新政, 樊永红. 棉秆固体发酵中木质素降解与酶活性变化的关联分析[J]. 新疆农业科学, 2023, 60(6): 1423-1432.

Figures/Tables 11

References 45

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固体发酵时期 Solid fermentation period	失重率 Weight loss rate (%)	纤维素含量 Cellulose content (%)	半纤维素含量 Hemicellulose content (%)	木质素含量 Lignin content (%)
初始 Initial stage	0.00^a	29.32±1.81^a	15.17±0.71^a	31.51±0.98^a
升温期 Warming period	5.86±1.05^b	28.47±1.49^a	13.30±0.67^b	31.45±0.88^a
高温期 High temperature period	6.42±0.9^b	28.36±0.87^a	11.48±0.16^c	30.60±1.19^ab
熟化期 Maturation period	29.30±0.75^c	21.27±0.38^b	8.42±0.15^d	27.52±1.25^ab
末期 Final stage	35.59±1.55^d	18.18±0.82^c	7.75±0.34^d	25.38±0.98^b

固体发酵时期 Solid fermentation period	失重率 Weight loss rate (%)	纤维素含量 Cellulose content (%)	半纤维素含量 Hemicellulose content (%)	木质素含量 Lignin content (%)
初始 Initial stage	0.00^a	29.32±1.81^a	15.17±0.71^a	31.51±0.98^a
升温期 Warming period	5.86±1.05^b	28.47±1.49^a	13.30±0.67^b	31.45±0.88^a
高温期 High temperature period	6.42±0.9^b	28.36±0.87^a	11.48±0.16^c	30.60±1.19^ab
熟化期 Maturation period	29.30±0.75^c	21.27±0.38^b	8.42±0.15^d	27.52±1.25^ab
末期 Final stage	35.59±1.55^d	18.18±0.82^c	7.75±0.34^d	25.38±0.98^b

指标 Index	发酵温度 Fermentation temperature	Lac活性 Lac activity	LiP活性 LiP activity	MnP活 MnP activity	木质素降解速率 Lignin degradation rate	纤维素降解速 Cellulose degradation rate	半纤维素降解速率 Hemicellulose degradation rate
发酵温度 Fermentation temperature	1
Lac活性Lac activity	-0.461	1
LiP活性LiP activity	-0.29	0.848^**	1
MnP活性MnP activity	-0.281	0.888^**	0.908^**	1
木质素降解速率 Lignin degradation rate	-0.088	0.611^**	0.698^**	0.695^**	1
纤维素降解速率 Cellulose degradation rate	0.344	0.467^*	0.477^*	0.617^**	0.666^**	1
半纤维素降解速率 Hemicellulose degradation rate	0.919^**	-0.363	-0.205	-0.164	-0.049	0.403	1

指标 Index	发酵温度 Fermentation temperature	Lac活性 Lac activity	LiP活性 LiP activity	MnP活 MnP activity	木质素降解速率 Lignin degradation rate	纤维素降解速 Cellulose degradation rate	半纤维素降解速率 Hemicellulose degradation rate
发酵温度 Fermentation temperature	1
Lac活性Lac activity	-0.461	1
LiP活性LiP activity	-0.29	0.848^**	1
MnP活性MnP activity	-0.281	0.888^**	0.908^**	1
木质素降解速率 Lignin degradation rate	-0.088	0.611^**	0.698^**	0.695^**	1
纤维素降解速率 Cellulose degradation rate	0.344	0.467^*	0.477^*	0.617^**	0.666^**	1
半纤维素降解速率 Hemicellulose degradation rate	0.919^**	-0.363	-0.205	-0.164	-0.049	0.403	1

成分 Components	初始特征值 Initial eigenvalue			提取平方和载入 Extraction sums of squared loadings
成分 Components	特征值 Eigenvalue	方差贡献率 Variance contribution rate (%)	累积方差贡献率 Cumulative variance contribution rate (%)	特征值 Eigenvalue	方差贡献率 Variance contribution rate (%)	累积方差贡献率 Cumulative variance contribution rate (%)
1	6.259	62.591	62.591	6.259	62.591	62.591
2	2.280	22.800	85.391	2.280	22.800	85.391
3	0.501	5.013	90.404
4	0.369	3.687	94.091
5	0.276	2.760	96.851
6	0.110	1.101	97.952
7	0.087	0.874	98.826
8	0.073	0.730	99.555
9	0.035	0.352	99.907
10	0.009	0.093	100.000

Correlation analysis of lignin degradation and enzyme activity changes in solid fermentation of cotton stalks

棉秆固体发酵中木质素降解与酶活性变化的关联分析

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Figures/Tables 11

References 45

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指标 Index	成分 Components
指标 Index	1	2
纤维素含量Cellulose content	-0.959	0.145
锰过氧化物酶活性Manganese peroxidase activity	0.957	0.070
半纤维素含量Hemicellulose content	-0.955	-0.187
木质素过氧化物酶活性Lignin peroxidases activity	0.931	-0.155
漆酶活性Laccase activity	0.909	0.007
木质素降解速率Lignin degradation rate	0.790	0.260
木质素含量Lignin content	-0.775	0.026
半纤维素降解速率Hemicellulose degradation rate	-0.278	0.918
温度Temperature	-0.362	0.898
纤维素降解速率Cellulose degradation rate	0.621	0.690

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