Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (2): 353-360.DOI: 10.6048/j.issn.1001-4330.2022.02.011
• Crop Genetics and Breeding·Molecular Genetics·Cultivation Physiology·Germplasm Resources • Previous Articles Next Articles
LI Junru(), QIN Ning, LI Wenlong, DU Hui(
), LI Xihuan, ZHANG Caiying
Received:
2021-06-09
Online:
2022-02-20
Published:
2022-03-22
Correspondence author:
DU Hui
Supported by:
李俊茹(), 秦宁, 李文龙, 杜汇(
), 李喜焕, 张彩英
通讯作者:
杜汇
作者简介:
李俊茹(1994-),女,河北张家口人,硕士研究生,研究方向为大豆遗传育种与分子生物学,(E-mail) 1451339484@qq.com
基金资助:
CLC Number:
LI Junru, QIN Ning, LI Wenlong, DU Hui, LI Xihuan, ZHANG Caiying. Identification of Seed Oligosaccharide and Its Components and Screening for Elite Germplasms in Soybean[J]. Xinjiang Agricultural Sciences, 2022, 59(2): 353-360.
李俊茹, 秦宁, 李文龙, 杜汇, 李喜焕, 张彩英. 大豆籽粒低聚糖及其组分含量鉴定与特异种质筛选[J]. 新疆农业科学, 2022, 59(2): 353-360.
低聚糖 Oligosaccharide | 回归方程 Linear regression equation | 决定系数 Coefficient of determination |
---|---|---|
蔗糖 Sucrose | Y=88 909X+177.6 | 0.999 7 |
棉籽糖 Raffinose | Y=70 846X+6445.9 | 0.993 6 |
水苏糖 Stachyose | Y=84 945X-6 584.3 | 0.999 8 |
Table 1 Linear regression equation and correlation coefficient of oligosaccharides
低聚糖 Oligosaccharide | 回归方程 Linear regression equation | 决定系数 Coefficient of determination |
---|---|---|
蔗糖 Sucrose | Y=88 909X+177.6 | 0.999 7 |
棉籽糖 Raffinose | Y=70 846X+6445.9 | 0.993 6 |
水苏糖 Stachyose | Y=84 945X-6 584.3 | 0.999 8 |
流动相配比 Ratio of mobile phase | 蔗糖Sucrose | 棉籽糖Raffinose | 水苏糖Stachyose | |||
---|---|---|---|---|---|---|
保留时间 Retention time | 理论塔板数 Theoretical plate | 保留时间 Retention time | 理论塔板数 Theoretical plate | 保留时间 Retention time | 理论塔板数 Theoretical plate | |
65∶35 | 5.583 | 5 342.550 | 7.035 | 5 975.969 | 9.085 | 6 199.417 |
70∶30 | 6.143 | 6 434.377 | 8.170 | 7 861.163 | 11.271 | 8 115.926 |
75∶25 | 8.725 | 6 758.959 | 14.315 | 8 833.943 | 24.685 | 9 113.752 |
Table 2 Results of different ratio of mobile phase’s experiment
流动相配比 Ratio of mobile phase | 蔗糖Sucrose | 棉籽糖Raffinose | 水苏糖Stachyose | |||
---|---|---|---|---|---|---|
保留时间 Retention time | 理论塔板数 Theoretical plate | 保留时间 Retention time | 理论塔板数 Theoretical plate | 保留时间 Retention time | 理论塔板数 Theoretical plate | |
65∶35 | 5.583 | 5 342.550 | 7.035 | 5 975.969 | 9.085 | 6 199.417 |
70∶30 | 6.143 | 6 434.377 | 8.170 | 7 861.163 | 11.271 | 8 115.926 |
75∶25 | 8.725 | 6 758.959 | 14.315 | 8 833.943 | 24.685 | 9 113.752 |
编号 Number | 蔗糖 Sucrose(%) | 棉籽糖 Raffinose(%) | 水苏糖 Stachyose(%) |
---|---|---|---|
1 | 5.25 | 0.45 | 3.13 |
2 | 5.26 | 0.46 | 3.17 |
3 | 5.28 | 0.46 | 3.11 |
4 | 5.30 | 0.47 | 3.11 |
5 | 5.31 | 0.46 | 3.07 |
6 | 5.24 | 0.46 | 3.13 |
均值 | 5.27 | 0.46 | 3.12 |
标准差 | 0.03 | 0.01 | 0.03 |
相对标准偏差 | 0.53 | 1.72 | 1.04 |
Table 3 The precision analysis of soybean oligosaccharides methods on technical duplication
编号 Number | 蔗糖 Sucrose(%) | 棉籽糖 Raffinose(%) | 水苏糖 Stachyose(%) |
---|---|---|---|
1 | 5.25 | 0.45 | 3.13 |
2 | 5.26 | 0.46 | 3.17 |
3 | 5.28 | 0.46 | 3.11 |
4 | 5.30 | 0.47 | 3.11 |
5 | 5.31 | 0.46 | 3.07 |
6 | 5.24 | 0.46 | 3.13 |
均值 | 5.27 | 0.46 | 3.12 |
标准差 | 0.03 | 0.01 | 0.03 |
相对标准偏差 | 0.53 | 1.72 | 1.04 |
编号 Number | 蔗糖 Sucrose(%) | 棉籽糖 Raffinose(%) | 水苏糖 Stachyose(%) |
---|---|---|---|
1 | 5.17 | 0.45 | 3.15 |
2 | 5.22 | 0.45 | 3.21 |
3 | 5.10 | 0.44 | 3.14 |
4 | 5.18 | 0.45 | 3.21 |
5 | 5.18 | 0.45 | 3.20 |
6 | 5.16 | 0.44 | 3.19 |
均值 | 5.17 | 0.45 | 3.18 |
标准差 | 0.04 | 0.01 | 0.03 |
相对标准偏差 | 0.76 | 1.24 | 0.98 |
Table 4 The precision analysis of soybean oligosaccharides methods on biological duplication
编号 Number | 蔗糖 Sucrose(%) | 棉籽糖 Raffinose(%) | 水苏糖 Stachyose(%) |
---|---|---|---|
1 | 5.17 | 0.45 | 3.15 |
2 | 5.22 | 0.45 | 3.21 |
3 | 5.10 | 0.44 | 3.14 |
4 | 5.18 | 0.45 | 3.21 |
5 | 5.18 | 0.45 | 3.20 |
6 | 5.16 | 0.44 | 3.19 |
均值 | 5.17 | 0.45 | 3.18 |
标准差 | 0.04 | 0.01 | 0.03 |
相对标准偏差 | 0.76 | 1.24 | 0.98 |
性状 Trait | 均值 Mean(%) | 变异范围 Range(%) | 标准差 SD(%) | 变异系数 CV(%) |
---|---|---|---|---|
蔗糖 Sucrose | 4.01 | 2.13~6.26 | 0.61 | 15.21 |
棉籽糖 Raffinose | 1.16 | 0.30~2.00 | 0.29 | 25.51 |
水苏糖 Stachyose | 2.60 | 0.95~3.87 | 0.50 | 19.16 |
低聚糖 Oligosaccharide | 7.77 | 5.63~11.13 | 0.70 | 9.04 |
Table 5 The genetic variation of oligosaccharide and its component contents in soybean varieties
性状 Trait | 均值 Mean(%) | 变异范围 Range(%) | 标准差 SD(%) | 变异系数 CV(%) |
---|---|---|---|---|
蔗糖 Sucrose | 4.01 | 2.13~6.26 | 0.61 | 15.21 |
棉籽糖 Raffinose | 1.16 | 0.30~2.00 | 0.29 | 25.51 |
水苏糖 Stachyose | 2.60 | 0.95~3.87 | 0.50 | 19.16 |
低聚糖 Oligosaccharide | 7.77 | 5.63~11.13 | 0.70 | 9.04 |
蔗糖 Sucrose | 棉籽糖 Raffinose | 水苏糖 Stachyose | 低聚糖 Oligosaccharide | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
组限 Group limits (%) | 次数 Number (个) | 频率 Percent (%) | 组限 Group limits (%) | 次数 Number (个) | 频率 Percent (%) | 组限 Group limits (%) | 次数 Number (个) | 频率 Percent (%) | 组限 Group limits (%) | 次数 Number (个) | 频率 Percent (%) |
1.99~2.27 | 1 | 0.30 | 0.24~0.36 | 1 | 0.30 | 0.85~1.04 | 1 | 0.30 | 5.45~5.82 | 2 | 0.60 |
2.27~2.54 | 1 | 0.30 | 0.36~0.47 | 1 | 0.30 | 1.04~1.24 | 2 | 0.60 | 5.82~6.18 | 2 | 0.60 |
2.54~2.82 | 2 | 0.60 | 0.47~0.58 | 4 | 1.19 | 1.24~1.43 | 3 | 0.89 | 6.18~6.55 | 10 | 2.98 |
2.82~3.09 | 16 | 4.76 | 0.58~0.70 | 6 | 1.79 | 1.43~1.63 | 2 | 0.60 | 6.55~6.91 | 13 | 3.87 |
3.09~3.37 | 26 | 7.74 | 0.70~0.81 | 28 | 8.33 | 1.63~1.82 | 10 | 2.98 | 6.91~7.28 | 46 | 13.69 |
3.37~3.64 | 44 | 13.10 | 0.81~0.92 | 38 | 11.31 | 1.82~2.02 | 20 | 5.95 | 7.28~7.65 | 68 | 20.24 |
3.64~3.92 | 65 | 19.35 | 0.92~1.03 | 43 | 12.80 | 2.02~2.22 | 35 | 10.42 | 7.65~8.01 | 79 | 23.51 |
3.92~4.20 | 63 | 18.75 | 1.03~1.15 | 50 | 14.88 | 2.22~2.41 | 45 | 13.39 | 8.01~8.38 | 68 | 20.24 |
4.20~4.47 | 46 | 13.69 | 1.15~1.26 | 50 | 14.88 | 2.41~2.61 | 44 | 13.10 | 8.38~8.75 | 29 | 8.63 |
4.47~4.75 | 32 | 9.52 | 1.26~1.37 | 40 | 11.90 | 2.61~2.80 | 55 | 16.37 | 8.75~9.11 | 9 | 2.68 |
4.75~5.02 | 23 | 6.85 | 1.37~1.49 | 32 | 9.52 | 2.80~3.00 | 49 | 14.58 | 9.11~9.48 | 2 | 0.60 |
5.02~5.30 | 7 | 2.08 | 1.49~1.60 | 16 | 4.76 | 3.00~3.19 | 31 | 9.23 | 9.48~9.85 | 2 | 0.60 |
5.30~5.57 | 5 | 1.49 | 1.60~1.71 | 15 | 4.46 | 3.19~3.39 | 19 | 5.65 | 9.85~10.21 | 3 | 0.89 |
5.57~5.85 | 3 | 0.89 | 1.71~1.83 | 6 | 1.79 | 3.39~3.58 | 13 | 3.87 | 10.21~10.58 | 1 | 0.30 |
5.85~6.13 | 1 | 0.30 | 1.83~1.94 | 3 | 0.89 | 3.58~3.78 | 6 | 1.79 | 10.58~10.94 | 1 | 0.30 |
6.13~6.40 | 1 | 0.30 | 1.94~2.05 | 3 | 0.89 | 3.78~3.97 | 1 | 0.30 | 10.94~11.31 | 1 | 0.30 |
Table 6 The distribution of oligosaccharide and its component contents in soybean varieties
蔗糖 Sucrose | 棉籽糖 Raffinose | 水苏糖 Stachyose | 低聚糖 Oligosaccharide | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
组限 Group limits (%) | 次数 Number (个) | 频率 Percent (%) | 组限 Group limits (%) | 次数 Number (个) | 频率 Percent (%) | 组限 Group limits (%) | 次数 Number (个) | 频率 Percent (%) | 组限 Group limits (%) | 次数 Number (个) | 频率 Percent (%) |
1.99~2.27 | 1 | 0.30 | 0.24~0.36 | 1 | 0.30 | 0.85~1.04 | 1 | 0.30 | 5.45~5.82 | 2 | 0.60 |
2.27~2.54 | 1 | 0.30 | 0.36~0.47 | 1 | 0.30 | 1.04~1.24 | 2 | 0.60 | 5.82~6.18 | 2 | 0.60 |
2.54~2.82 | 2 | 0.60 | 0.47~0.58 | 4 | 1.19 | 1.24~1.43 | 3 | 0.89 | 6.18~6.55 | 10 | 2.98 |
2.82~3.09 | 16 | 4.76 | 0.58~0.70 | 6 | 1.79 | 1.43~1.63 | 2 | 0.60 | 6.55~6.91 | 13 | 3.87 |
3.09~3.37 | 26 | 7.74 | 0.70~0.81 | 28 | 8.33 | 1.63~1.82 | 10 | 2.98 | 6.91~7.28 | 46 | 13.69 |
3.37~3.64 | 44 | 13.10 | 0.81~0.92 | 38 | 11.31 | 1.82~2.02 | 20 | 5.95 | 7.28~7.65 | 68 | 20.24 |
3.64~3.92 | 65 | 19.35 | 0.92~1.03 | 43 | 12.80 | 2.02~2.22 | 35 | 10.42 | 7.65~8.01 | 79 | 23.51 |
3.92~4.20 | 63 | 18.75 | 1.03~1.15 | 50 | 14.88 | 2.22~2.41 | 45 | 13.39 | 8.01~8.38 | 68 | 20.24 |
4.20~4.47 | 46 | 13.69 | 1.15~1.26 | 50 | 14.88 | 2.41~2.61 | 44 | 13.10 | 8.38~8.75 | 29 | 8.63 |
4.47~4.75 | 32 | 9.52 | 1.26~1.37 | 40 | 11.90 | 2.61~2.80 | 55 | 16.37 | 8.75~9.11 | 9 | 2.68 |
4.75~5.02 | 23 | 6.85 | 1.37~1.49 | 32 | 9.52 | 2.80~3.00 | 49 | 14.58 | 9.11~9.48 | 2 | 0.60 |
5.02~5.30 | 7 | 2.08 | 1.49~1.60 | 16 | 4.76 | 3.00~3.19 | 31 | 9.23 | 9.48~9.85 | 2 | 0.60 |
5.30~5.57 | 5 | 1.49 | 1.60~1.71 | 15 | 4.46 | 3.19~3.39 | 19 | 5.65 | 9.85~10.21 | 3 | 0.89 |
5.57~5.85 | 3 | 0.89 | 1.71~1.83 | 6 | 1.79 | 3.39~3.58 | 13 | 3.87 | 10.21~10.58 | 1 | 0.30 |
5.85~6.13 | 1 | 0.30 | 1.83~1.94 | 3 | 0.89 | 3.58~3.78 | 6 | 1.79 | 10.58~10.94 | 1 | 0.30 |
6.13~6.40 | 1 | 0.30 | 1.94~2.05 | 3 | 0.89 | 3.78~3.97 | 1 | 0.30 | 10.94~11.31 | 1 | 0.30 |
性状 Trait | 蔗糖 Sucrose | 棉籽糖 Raffinose | 水苏糖 Stachyose |
---|---|---|---|
棉籽糖 Raffinose | 0.351** | ||
水苏糖 Stachyose | -0.415** | -0.298** | |
低聚糖 Oligosaccharide | 0.721** | 0.513** | 0.224** |
Table 7 The correlation analysis of oligosaccharide and its component contents in soybean
性状 Trait | 蔗糖 Sucrose | 棉籽糖 Raffinose | 水苏糖 Stachyose |
---|---|---|---|
棉籽糖 Raffinose | 0.351** | ||
水苏糖 Stachyose | -0.415** | -0.298** | |
低聚糖 Oligosaccharide | 0.721** | 0.513** | 0.224** |
类别 Type | 品种 Representative variety | 品种个数 Number | 低聚糖含量均值 Mean of Oligosaccharide(%) |
---|---|---|---|
Ⅰ | 吉林3号、大粒黄、青杂豆、黑河9号、呼交282 | 9 | 10.08 |
合丰29、黑河1号、宝丰8号、疆丰23-3412、 | |||
Ⅱ | 六十天还家、海伦嘟噜豆、小白豆、桦南小金豆、宝青绿大豆、 | 323 | 7.72 |
茶秣食豆、黑鉴1、合丰26、绥农6、褐脐、蓑衣领、涝洲白脐大豆 | |||
蛟河紫花1、蓝脐、舒兰满仓金、伊通满仓金、大红脐黑豆、黑大豆、 | |||
绿瓤黑豆、方正秣食豆、小金黄1、金元1、哈1、小白脐、珲春豆、 | |||
吉育47、东农4号、丰收1号、黑河3号、黑河51、黑河54、牡丰1号、 | |||
元宝金、荆山扑、毛豆、羊角蜜、采种圃、双城4、压破车、白毛霜等 | |||
Ⅲ | 黑秣食豆、黑秣豆、吉育105、哈12-4547 | 4 | 6.54 |
Table 8 The cluster analysis of oligosaccharide and its component contents in soybean
类别 Type | 品种 Representative variety | 品种个数 Number | 低聚糖含量均值 Mean of Oligosaccharide(%) |
---|---|---|---|
Ⅰ | 吉林3号、大粒黄、青杂豆、黑河9号、呼交282 | 9 | 10.08 |
合丰29、黑河1号、宝丰8号、疆丰23-3412、 | |||
Ⅱ | 六十天还家、海伦嘟噜豆、小白豆、桦南小金豆、宝青绿大豆、 | 323 | 7.72 |
茶秣食豆、黑鉴1、合丰26、绥农6、褐脐、蓑衣领、涝洲白脐大豆 | |||
蛟河紫花1、蓝脐、舒兰满仓金、伊通满仓金、大红脐黑豆、黑大豆、 | |||
绿瓤黑豆、方正秣食豆、小金黄1、金元1、哈1、小白脐、珲春豆、 | |||
吉育47、东农4号、丰收1号、黑河3号、黑河51、黑河54、牡丰1号、 | |||
元宝金、荆山扑、毛豆、羊角蜜、采种圃、双城4、压破车、白毛霜等 | |||
Ⅲ | 黑秣食豆、黑秣豆、吉育105、哈12-4547 | 4 | 6.54 |
类型 Type | 低聚糖 Oligosaccharide(%) | 蔗糖 Sucrose(%) | 棉籽糖 Raffinose(%) | 水苏糖 Stachyose(%) |
---|---|---|---|---|
高含量 | 合丰29(10.01) | 吉林3号(5.59) | 黑河21(1.86) | 辽08012(3.64) |
High content | 疆丰23-3412(10.21) | 呼交282(5.69) | 呼交282(1.92) | 合丰38(3.69) |
青杂豆(10.28) | 宝丰8号(5.81) | 疆丰23-3412(1.95) | 黑秣食豆(3.70) | |
大粒黄(10.64) | 大粒黄(5.89) | 毛豆(1.98) | 黑秣豆(3.77) | |
呼交282(11.13) | 青杂豆(6.26) | 合丰44(2.00) | 哈14-2146(3.87) | |
低含量 | 宾县黑豆(5.63) | 哈12-4547(2.13) | 黑秣食豆(0.30) | 绥无腥豆3号(0.95) |
Low content | 哈12-4547(5.79) | 吉育105(2.43) | 吉育105(0.45) | 合农69(1.08) |
绥农1号(6.12) | 黑秣豆(2.62) | 吉育86(0.51) | 黑河27(1.26) | |
东农绿芽豆1号(6.14) | 东农绿芽豆1号(2.64) | 青秣食豆(0.56) | 丰收10号(1.32) | |
绥农20(6.29) | 金山茶秣食豆(2.85) | 龙泉大豆(0.56) | 宾县黑豆(1.34) |
Table 9 The selected soybean germplasms of oligosaccharide and its component contents
类型 Type | 低聚糖 Oligosaccharide(%) | 蔗糖 Sucrose(%) | 棉籽糖 Raffinose(%) | 水苏糖 Stachyose(%) |
---|---|---|---|---|
高含量 | 合丰29(10.01) | 吉林3号(5.59) | 黑河21(1.86) | 辽08012(3.64) |
High content | 疆丰23-3412(10.21) | 呼交282(5.69) | 呼交282(1.92) | 合丰38(3.69) |
青杂豆(10.28) | 宝丰8号(5.81) | 疆丰23-3412(1.95) | 黑秣食豆(3.70) | |
大粒黄(10.64) | 大粒黄(5.89) | 毛豆(1.98) | 黑秣豆(3.77) | |
呼交282(11.13) | 青杂豆(6.26) | 合丰44(2.00) | 哈14-2146(3.87) | |
低含量 | 宾县黑豆(5.63) | 哈12-4547(2.13) | 黑秣食豆(0.30) | 绥无腥豆3号(0.95) |
Low content | 哈12-4547(5.79) | 吉育105(2.43) | 吉育105(0.45) | 合农69(1.08) |
绥农1号(6.12) | 黑秣豆(2.62) | 吉育86(0.51) | 黑河27(1.26) | |
东农绿芽豆1号(6.14) | 东农绿芽豆1号(2.64) | 青秣食豆(0.56) | 丰收10号(1.32) | |
绥农20(6.29) | 金山茶秣食豆(2.85) | 龙泉大豆(0.56) | 宾县黑豆(1.34) |
[1] | 秦振顺, 陈桂茹, 常凤启, 等. 大豆及其制品中大豆低聚糖的测定方法[J]. 中国卫生工程学, 2002, 1(2):34-35. |
QIN Zhengshun, CHEN Guiru, CHANG Fengqi, et al. Determination of oligosaccharides in soybean and its products[J]. Chinese Journal of Public Health Engineering, 2002, 1(2):34-35. | |
[2] | 向小丽, 杨立怡, 孙怡, 等. 高效液相色谱法分析大豆和大豆低聚糖浆中糖的组成与含量[J]. 食品科学, 2007, 28(12):412-415. |
XIANG Xiaoli, YANG Liyi, SUN Yi, et al. Analysis of Free Sugars in Soybean Seed and Soybean Oligosaccharide Syrup by High Performance Liquid Chromatography[J]. Food Science, 2007, 28(12):412-415. | |
[3] | 李文治, 杨连生, 黄强, 等. 大豆低聚糖研究进展[J]. 粮食与油脂, 2002, (7):50-51. |
LI Wenzhi, YANG Liansheng, HUANG Qiang, et al. Advanced research of soybean oligosaccharides[J]. Cereals & Oils, 2002, (7):50-51. | |
[4] | 张延坤, 张东样, 刘国忠, 等. 高效液相色谱法测定大豆低聚糖[J]. 食品工业, 2000, (2):45-46. |
ZHANG Yankun, ZHANG Dongxiang, LIU Guozhong, et al. Determination of soybean oligosaccharides by high performance liquid chromatography[J]. The food Science, 2000, (2):45-46. | |
[5] | 潘城, 欧阳立群, 林钦, 等. 高效液相色谱-示差折光法测定饲料中大豆低聚糖含量[J]. 中国饲料, 2015, (5):32-36. |
PAN Cheng, OUYANG Liqun, LIN Qin, et al. Determination of soybean oligosaccharides in feed by HIGH performance liquid chromatography-differential refractive index methods[J]. China Feed, 2015, (5):32-36. | |
[6] | 吴朝阳, 马玲云, 魏锋, 等. RP-HPLC-RID法测定保健食品中大豆低聚糖的含量[J]. 药物分析杂志, 2010, 30(11):2081-2085. |
WU Zhaoyang, MA Lingyun, WEI Feng, et al. Determination of soybean oligosaccharide in health foods by RP-HPLC-RID[J]. Chinese journal of Pharmaceutical Analysis, 2010, 30(11):2081-2085. | |
[7] | 黄美. 我国大豆地方品种群体籽粒可溶性糖含量的遗传变异和关联定位研究[D]. 南京: 南京农业大学, 2012. |
HUANG Mei. Genetic variability and QTL association mapping of seed soluble sugar content in Chinese landrace population of soybean[D]. Nanjing: Nanjing Agricultural University, 2012. | |
[8] | 王玉军, 纪伟东, 李永平, 等. 大豆低聚糖粉中棉子糖和水苏糖的测定方法研究[J]. 现代食品科技, 2010, (4):23-25. |
WANG Yujun, JI Weidong, LI Yongping, et al. Study on the determination of raffinose and stachyose in soybean oligosaccharide powder[J]. Modern Food Science & Technology, 2010,(4):23-25. | |
[9] | 程云辉, 贾振宝. 大豆低聚糖的研究进展[J]. 食品与机械, 2004, (6):57-60. |
CHENG Yunhui, JIA Zhenbao. Research progress in soybean oligosaccharides[J]. Food & Machinery, 2004, (6):57-60. | |
[10] | 李怡然, 赵丽芹, 贠婷婷, 等. 常见大豆制品中水溶性生物活性物质的分析[J]. 中国食品学报, 2016, 16(2):258-265. |
LI Yiran, ZHAO Liqin, YUN Tingting, et al. Analysis of Water-soluble Bioactive Substances in Soybean Products[J]. Journal of Chinese Institute of Food Science and Technology, 2016, 16(2):258-265. | |
[11] | 孟繁磊, 魏春雁, 宋志峰, 等. HPLC-ELSD测定大豆中水溶性糖含量[J]. 食品科技, 2016, 41(10):258-261. |
MENG Fanlei, WEI Chunyan, SONG Zhifeng, et al. Determination of content of free sugar in soybean by HPLC-ELSD[J]. Food Science and Technology, 2016, 41(10):258-261. | |
[12] | 宋志峰, 金卫东, 王丽, 等. 吉林省部分大豆品种(系)低聚糖含量分析[J]. 大豆科学, 2009, 28(3):516-523. |
SONG Zhifeng, JIN Weidong, WANG Li, et al. Determination of Oligosaccharides in Some Soybean Varieties (Lines) in Jilin Province[J]. Soybean Science, 2009, 28(3):516-523. | |
[13] | 王晓岩, 郝再彬, 邱丽娟, 等. 大豆Harosoy近等基因系低聚糖及其组分含量的变异分析[J]. 植物遗传资源学报, 2010, 11(5):527-534. |
WANG Xiaoyan, HAO Zaibin, QIU Lijuan, et al. The Analysis of Variation with Oligosaccharide and ItsComponent Contents of Harosoy Isolines[J]. Journal of Plant Genetic Resources, 2010, 11(5):527-534. | |
[14] | 王曙明, 胡明祥, 胡传璞, 等. 吉林省大豆品质资源低聚糖含量的初步分析[J]. 吉林农业科学, 1990, (1):92-94. |
WANG Shuming, HU Mingxiang, HU Chuanpu, et al. Preliminary analysis of oligosaccharide content of soybean quality resources in Jilin Province[J]. Journal of Jilin Agricultural Sciences, 1990, (1):92-94. | |
[15] |
Yoshikawa Y, Chen P, Zhang B, et al. Evaluation of seed chemical quality traits and sensory properties of natto soybean[J]. Food Chemistry, 2014, 153(6):186-192.
DOI URL |
[16] | 王跃强. 大豆低聚糖与白粉病的遗传分析及相关基因分子标记[D]. 哈尔滨: 东北农业大学, 2012. |
WANG Yueqiang. Study on genetic analysis and molecular markers’ mapping of soybean oligosaccharides and powdery mildew[D]. Harbin: Northeast Agricultural University, 2012. | |
[17] | Kerr P S, Sebastian S A. Soybean products with improved carbohydrate composition and soybean plants [P]. U. S. Patent, 2000, (34):1123-1132. |
[18] |
Neus J D, Fehr W R, Schnebly S R. Agronomic and seed characteristics of soybean with reduced raffinose and stachyose[J]. Crop Science, 2005, 45(2):589-592.
DOI URL |
[19] | 王晓岩, 郝再彬, 邱丽娟. HPLC法快速检测大豆籽粒中大豆低聚糖的含量[J]. 食品科技, 2010, 35(7):287-290. |
WANG Xiaoyan, HAO Zaibin, QIU Lijuan. The method for rapid detection of soybean oligosaccharidecontent by HPLC[J]. Food Science and Technology, 2010, 35(7):287-290. | |
[20] | 宋春丽, 王文侠, 曾风彩, 等. 超声波和微波辅助提取大豆低聚糖的工艺比较[J]. 食品与机械, 2011, 27(2):47-50. |
SONG Chunli, WANG Wenxia, ZENG Fengcai, et al. Comparison of ultrasonic-assisted and microwave-assisted extraction of oligosaccharides from high denatured soybean meal[J]. Food & Machinery, 2011, 27(2):47-50. | |
[21] | 张昌军, 原方圆, 邵红兵, 等. 超声波法在提取多糖类化合物中的应用研究[J]. 化工时刊, 2007, (2):54-56. |
ZHANG Changjun, YUAN Fangyuan, SHAO Hongbing, et al. Application of Extracting Polysaccharide Compound by Ultrasonic Method[J]. Chemical Industry Times, 2007, (2):54-56. | |
[22] | 詹晓丹. 大豆低聚糖检测方法优化初探[J]. 现代畜牧科技, 2018, (3):14. |
ZHAN Xiaodan. Preliminary Study on optimization of detection Method for soybean oligosaccharides[J]. Modern animal Husbandry Science & Technology, 2018, (3):14. | |
[23] | 郝岩平, 姜金斗, 杨秀茹. HPLC法测定食品中大豆低聚糖的含量[J]. 中国甜菜糖业, 2003, (1):8-11. |
HAO Yanping, JIANG Jindou, YANG Xiuru. Determination of Soybean Oligosaccharide in Food by HPLC[J]. China Beet & Sugar, 2003, (1):8-11. | |
[24] | 刘立洋, 金龙国, 刘章雄, 等. 微波和超声两种技术提取大豆低聚糖的效果[J]. 大豆科学, 2008, 27(5):843-844. |
LIU Liyang, JIN Longguo, LIU Zhangxiong, et al. Extraction of Soybean Oligosaccharides by Microwave and Ultrasonic Technique[J]. Soybean Science, 2008, 27(5):843-844. | |
[25] | 王晓岩. 大豆低聚糖含量检测技术及其应用研究[D]. 桂林: 桂林理工大学, 2007. |
WANG Xiaoyan. The Detection Technology of Soybean Oligosaccharide Content and Its Applied Research[D]. Guilin: Guilin University of Technology, 2007. |
[1] | YANG Xiangbo, CHEN Liangyu, YANG Songnan, CHEN Xifeng, XING Weiming, LI Xueying, CONG Weixuan, ZANG Zhenyuan, ZANG Yuanbo, ZHANG Jun. Phenotype analysis and comprehensive evaluation of spring soybean germplasm resources from northeast China [J]. Xinjiang Agricultural Sciences, 2024, 61(12): 2921-2933. |
[2] | SHI Xiaolei, DING Sunlei, CONG Hua, ZHANG Jinbo, QU Kejia, WANG Xingzhou, HAN dai, YAN Yongliang. Gray correlation analysis of reast soybean yield correlation traits [J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1641-1652. |
[3] | YAN Yongliang, ZHANG Heng, QU Kejia, SHI Xiaolei, WANG Xingzhou, ZHANG Jinbo, CONG Hua. Comparative study on the agronomic traits and yields of different soybean varieties [J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1653-1662. |
[4] | ZHAO Lianjia, LI Gan, XU Lin, YAN Guorong, LIU Ning, WANG Fan, DENG Chaohong, Abudukeyoumu Abudurezike, WANG Cong, WANG Wei. Analysis of the main characters of soybean varieties in Xinjiang and their correlation with yield [J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1663-1670. |
[5] | ZHU Baoguo, KUANG Enjun, TENG Zhanglin, MENG Qingying, WANG Nannan, FENG Haoyuan, QIU Lei, GAO Xuedong, ZHANG Chunfeng. Effects of different bio-organic fertilizers application combined with conventional fertilization on growth, disease resistance and yield of soybean [J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1127-1133. |
[6] | ZHANG Jianxun, REN Jintao, WU Haijun, WANG Cong, ZHANG Jianxin. Effect of Densities on Flower and Pod Formation and Yield of Spring Soybean Varieties with Different Plant Types [J]. Xinjiang Agricultural Sciences, 2023, 60(3): 539-546. |
[7] | Alibieligen Hazitai, Hanipa Hazaisi, Zulehumaer Wusimanjiang, YAO Qing, Shouhereti Yodika, HOU Xanfei, LI Qiang, ZHANG Zheng. Adaptability Analysis of Introduced Soybean Varieties in Yili Valley of Xinjiang [J]. Xinjiang Agricultural Sciences, 2023, 60(2): 326-335. |
[8] | YI Yuanyang, XIE Fang, TIAN Shiying, ZHANG Zhidong, GU Meiying, PENG Xiaowu. Effect of Organic Fertilizer of Thioerythromycin Residue on Soybean Soil Drug-resistant Bacteria and Their Resistance Genes [J]. Xinjiang Agricultural Sciences, 2023, 60(2): 424-431. |
[9] | WU Shu, XU Yue, XU Yaxin, HUANG Xingjun, WU Quanzhong, CHEN Guodong, ZHAI Yunlong. Effects of Rhizobium Inoculation Mode on Growth and Development of Multiple Soybean [J]. Xinjiang Agricultural Sciences, 2023, 60(1): 52-60. |
[10] | XU Yue, LI Ling, XU Yaxin, HUANG Xingjun, WU Quanzhong, CHEN Guodong, ZHAI Yunlong. Effects of Rhizobium Inoculation Methods on Growth and Nodulation of Multiple Sowing Soybeans [J]. Xinjiang Agricultural Sciences, 2022, 59(4): 810-817. |
[11] | ZHONG Haojie, YAO Xinkui, LUO Penghui, MENG Jun, YAO Yueyang, WANG Chuankun, REN Wanlu. Effects of Supplemental Fermented Soybean Meal on Fecal Metabolites in Yili Foal Based on Metabolomics Analysis [J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2800-2807. |
[12] | DAI Xiaohua, GU Hongfei, JIN Kexu, SHU Jiamin, YANG Ruolan, WU Menglan, PAN Hui, ZHAO Hongqiong. Determination of Flavonoids in Wild Apricot Flesh by RP-HPLC [J]. Xinjiang Agricultural Sciences, 2021, 58(8): 1476-1485. |
[13] | GAO Yufang, ZHANG Yanjun, ZHAO Zhenning, ZHAO Baoxie, DU Shikun. Adaptability and Genetic Diversity Analysis of Introduced Soybean Germplasm Resources [J]. Xinjiang Agricultural Sciences, 2021, 58(6): 1029-1041. |
[14] | DU Xiaojing, AN Chongxiao, XU Wenxiu, TANG Jianghua, FANG Yanfei, ZHANG Na, LI Ling. Effects of Different Amounts of Drip Irrigation under Mulch Film on Soil Respiration and Organic Carbon in Soybean Field [J]. Xinjiang Agricultural Sciences, 2021, 58(3): 457-466. |
[15] | Qiong LI, Shihao CHANG, Wentao SHU, Qingchun YANG, Jinhua LI, Donghui ZHANG, Baoliang ZHANG, Laicheng ZHANG, Zhen GENG. Comprehensive Analysis of Agronomic Characters of 46 Soybean Varieties (Lines) of Summer Soybeans (South) in Huang-huai-hai Region [J]. Xinjiang Agricultural Sciences, 2021, 58(10): 1765-1774. |
Viewed | ||||||||||||||||||||||||||||||||||||||||||||||||||
Full text 48
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||
Abstract 1005
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||