Identification of Pathogens Causing Leaf Spot Disease at Cotton Seedling Stage in Shihezi Area

doi:10.6048/j.issn.1001-4330.2017.11.013

Xinjiang Agricultural Sciences ›› 2017, Vol. 54 ›› Issue (11): 2060-2066.DOI: 10.6048/j.issn.1001-4330.2017.11.013

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Identification of Pathogens Causing Leaf Spot Disease at Cotton Seedling Stage in Shihezi Area

LI Ying-cheng, ZHANG Guo-li, YANG Chao, REN Yu-zhong, LI Zhuo, ZHANG Li, LI Guo-ying

College of Agronomy, Shihezi University / Key Laboratory for Oasis Agricultural Pest Management and Plant Resource Utilization at Universities of Xinjiang Uygur Autonomous Region, Shihezi Xinjiang 832003, China

Received:2017-08-28 Online:2017-11-20 Published:2017-12-06
Correspondence author: LI Guo-ying(1941-), male, native place: Hebei, Professor, research field: plant pathogenic fungi and fungal diseases. (E-mail)lgyshzu@163.com;ZHANG Li(1970-), female, native place: Jiangsu, Ph. D., research field: plant pathology. (E-mail)1602784618@qq.com
Supported by:
Selection of High-yield and Fine-quality Peanuts Varieties and Study on the Cultivation(2016B01008-2)

石河子地区棉花苗期叶斑病病原菌种类鉴定

李映程, 张国丽, 杨超, 任毓忠, 李卓, 张莉, 李国英

石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用自治区高校重点实验室,新疆石河子 832003

通讯作者: 李国英(1941-),男,河北人,教授,研究方向为植物病原真菌和真菌病害,(E-mail)lgyshzu@163.com;张莉(1970-),女,江苏人,教授,博士,研究方向为植物病理学,(E-mail)1602784618@qq.com
作者简介:李映程(1994-),男,甘肃人,硕士,研究方向为植物病理学,(E-mail)1363731850@qq.com
基金资助:
科技部中小企业创新项目(14C26216513812)

Abstract

Abstract: 【Objective】 The aim of the study is to verify the pathogens that cause cotton seedling leaf spot in Shihezi area.【Method】120 samples of cotton leaf spot were collected from Shihezi area, and 105 strains of Alternaria spp were isolated and purified by conventional method, and 15 representative strains were identified through survey of morphological characteristics, test of pathogenicity and sequence analysis of histone 3 gene.【Result】The selected 15 representative strains were pathogenic. The pathogens were preliminarily identified as A.alternata and A.tenuissima according to morphological characteristics, respectively. Based on histone 3 gene sequence analysis, the results showed that the similarity of nucleotide homogeneity of A.alternata (AF404620, KF997067 and KR866858) was 99% and A.tenuissima (KT384348) was more than 99%, respectively. 【Conclusion】The pathogens causing cotton seedling leaf spot are A.alternata and A.tenuissima in Shihezi in 2016.

Key words: cotton seedling leaf spot; pathogen; identification

摘要： 【目的】研究石河子地区棉花苗期叶斑病病原菌种类。【方法】棉花苗期,采集石河子地区典型叶斑病样120个,用常规组织分离法进行分离和纯化,共得到105个链格孢属(Alternaria spp.)菌株,选取其中15个代表性菌株,通过致病性测定、常规形态学鉴定及组蛋白3基因序列分析,对病原菌种类进行鉴定。【结果】供试15个代表性菌株均具有致病性。形态学测定,可将其鉴定为A.alternata和A.tenuissima两类;基于组蛋白3基因序列分析,前一类与A.alternata(AF404620、KF997067和KR866858)相似性达99%,后一类与A.tenuissima(KT384348)相似性达99%以上。【结论】2016年引起石河子地区棉花苗期叶斑病的病原菌为A.alternata和A.tenuissima。

关键词: 棉苗叶斑病, 病原菌, 鉴定

CLC Number:

S435.621

LI Ying-cheng, ZHANG Guo-li, YANG Chao, REN Yu-zhong, LI Zhuo, ZHANG Li, LI Guo-ying. Identification of Pathogens Causing Leaf Spot Disease at Cotton Seedling Stage in Shihezi Area[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2060-2066.

李映程, 张国丽, 杨超, 任毓忠, 李卓, 张莉, 李国英. 石河子地区棉花苗期叶斑病病原菌种类鉴定[J]. 新疆农业科学, 2017, 54(11): 2060-2066.

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Identification of Pathogens Causing Leaf Spot Disease at Cotton Seedling Stage in Shihezi Area

石河子地区棉花苗期叶斑病病原菌种类鉴定

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