不同马铃薯耐盐性转录组比较及耐盐基因的挖掘

doi:10.6048/j.issn.1001-4330.2025.05.009

新疆农业科学 ›› 2025, Vol. 62 ›› Issue (5): 1121-1130.DOI: 10.6048/j.issn.1001-4330.2025.05.009

• 作物遗传育种·耕作栽培·生理生化 • 上一篇下一篇

不同马铃薯耐盐性转录组比较及耐盐基因的挖掘

王亚玲(), 江应红, 孙慧, 刘易()

新疆维吾尔自治区农业科学院乌鲁木齐综合试验站,乌鲁木齐 830012

收稿日期:2024-10-07 出版日期:2025-05-20 发布日期:2025-07-09
通信作者: 刘易(1983-),男,河北保定人,副研究员,博士研究生,研究方向为作物栽培育种,(E-mail)liuyun_5511@163.com
作者简介:王亚玲(1994-),女,新疆乌鲁木齐人,助理研究员,博士研究生,研究方向为马铃薯抗逆育种,(E-mail)yalingwangx@163.com
基金资助:
新疆维吾尔自治区自然科学青年基金项目“基于转录组测序马铃薯耐盐基因的挖掘”(2021D01B69);新疆维吾尔自治区重点研发任务专项“新疆马铃薯产业提质增效关键技术研究”(2022B02044);国家马铃薯产业技术体系-乌鲁木齐综合试验站(CARS-09-ES36)

Comparative transcriptome analysis between two potato varieties with different salt-tolerance and further identification of potato salt-tolerance genes

WANG Yaling(), JIANG Yinghong, SUN Hui, LIU Yi()

Urumqi Comprehensive Experimental Station Xinjiang Uyghur Autonomous Region Academy of Agriculture Sciences, Urumqi 830012, China

Received:2024-10-07 Published:2025-05-20 Online:2025-07-09
Supported by:
Natural Science Foundation of Xinjiang Uyghur Autonomous Region “Comparative transcriptome analysis between two potato varieties with different salt-tolerance and further identification of potato salt-tolerance genes”(2021D01B69);Project of the Key R & D Special Task of Xinjiang Uygur Autonomous Region “Research on Key Technologies for Quality Improvement and Efficiency Enhancement in Potato Industry”(2022B02044);China Agriculture Research System — Urumqi Comprehensive Experimental Station(CARS-09-ES36)

摘要/Abstract

摘要：

【目的】研究与马铃薯耐盐性相关的候选基因,为马铃薯耐盐性鉴定与评价和耐盐基因的挖掘提供理论依据。【方法】以耐盐品种晋薯16号及盐敏感品种冀张薯12号为试材,采用0%、0.5%、1%和1.5% NaCl溶液进行盐胁迫处理,在第8 d时,分析植株表型和生理生化指标,随后选择1%的盐浓度处理,分析2个品种进行转录组测序和生物信息学。【结果】相较于冀张薯12号,晋薯16号在盐胁迫条件下,具有较低的丙二醛含量。植物激素信号转导途径和类黄酮代谢途径在2个马铃薯品种中显示出明显的差异。【结论】筛选出与植物激素信号转导和类黄酮代谢相关的差异表达基因。

关键词: 马铃薯; 盐胁迫; 转录组测序; 植物激素信号途径; 类黄酮代谢途径

Abstract:

【Objective】 To discover candidate genes related to salt tolerance in potatoes and provide theoretical basis for the identification and evaluation of salt tolerance in potatoes and the exploration of salt tolerance genes. 【Methods】 The salt tolerant variety Jinshu 16 and salt sensitive variety Jizhangshu 12 were used as test materials. Salt stress treatments were carried out using 0%, 0.5%, 1%, and 1.5% NaCl solutions. On the 8th day, plant phenotypes and physiological and biochemical indicators were analyzed. Subsequently, a 1% salt concentration was selected for treatment, and transcriptome sequencing and bioinformatics analysis were performed on the two varieties. 【Results】 Compared to Jizhangshu 12, Jinshu 16 had lower malondialdehyde content under salt stress conditions. Transcriptome data showed significant differences in plant hormone signaling pathways and flavonoid metabolism pathways between the two varieties. 【Conclusion】 Based on the above results, several differentially expressed genes related to plant hormone signal transduction and flavonoid metabolism are screened, which provides genetic resources for improving salt tolerance in potatoes.

Key words: potato; salt stress; transcriptome sequencing; plant hormone signaling pathways; flavonoids metabolic pathways

中图分类号:

S532

王亚玲, 江应红, 孙慧, 刘易. 不同马铃薯耐盐性转录组比较及耐盐基因的挖掘[J]. 新疆农业科学, 2025, 62(5): 1121-1130.

WANG Yaling, JIANG Yinghong, SUN Hui, LIU Yi. Comparative transcriptome analysis between two potato varieties with different salt-tolerance and further identification of potato salt-tolerance genes[J]. Xinjiang Agricultural Sciences, 2025, 62(5): 1121-1130.

图/表 9

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基因号 Gene ID	上游引物 Up-stream primer	下游引物 Down-stream primer
PGSC0003DMT400016742	CTGTTGTGGCCGTCCTAACT	ATCAGGCTTGTGGTCAGTGG
PGSC0003DMT400018004	ATGGAAGAGATGTTTCCAGCACT	GCAAGCTAGTCGTATTCGGG
PGSC0003DMT400027196	GTGCGACTTCGGTATGTGGA	AGCCATGGCCATCGTAAACA
PGSC0003DMT400029264	AAGTCGGTGGAGACTTATGCTT	AGCACGGATTAAAGCCCTC
StEF1a	GATGTTGTGCCAAAGGATGT	AACTTGTGGTCAATGCGAGA

基因号 Gene ID	上游引物 Up-stream primer	下游引物 Down-stream primer
PGSC0003DMT400016742	CTGTTGTGGCCGTCCTAACT	ATCAGGCTTGTGGTCAGTGG
PGSC0003DMT400018004	ATGGAAGAGATGTTTCCAGCACT	GCAAGCTAGTCGTATTCGGG
PGSC0003DMT400027196	GTGCGACTTCGGTATGTGGA	AGCCATGGCCATCGTAAACA
PGSC0003DMT400029264	AAGTCGGTGGAGACTTATGCTT	AGCACGGATTAAAGCCCTC
StEF1a	GATGTTGTGCCAAAGGATGT	AACTTGTGGTCAATGCGAGA

样品 Sample	有效序列 Clean reads	有效碱基(G) Clean bases	Q30 含量 Q30 content (%)	GC 含量 GC content (%)	比对效率 Mapped reads ratio (%)
JC	42058489	6.27	93.89	43.89	80.01
JT	41683414	6.26	93.67	43.98	85.13
ZC	41728022	6.22	93.62	43.36	87.58
ZT	41015686	6.12	93.71	43.95	87.72

样品 Sample	有效序列 Clean reads	有效碱基(G) Clean bases	Q30 含量 Q30 content (%)	GC 含量 GC content (%)	比对效率 Mapped reads ratio (%)
JC	42058489	6.27	93.89	43.89	80.01
JT	41683414	6.26	93.67	43.98	85.13
ZC	41728022	6.22	93.62	43.36	87.58
ZT	41015686	6.12	93.71	43.95	87.72

名称 Name	基因ID Gene ID	表达模式 Expression pattern
蛋白激酶 Protein kinase	PGSC0003DMG400003790 PGSC0003DMG400009843 PGSC0003DMG400014064 PGSC0003DMG400015666 PGSC0003DMG400017943 PGSC0003DMG400018840 PGSC0003DMG400019720 PGSC0003DMG400021993 PGSC0003DMG401019681 PGSC0003DMG402029906	下调 Down
PP2C Protein phosphatase 2C	PGSC0003DMG400002573 PGSC0003DMG400009112 PGSC0003DMG400016742 PGSC0003DMG400018004 PGSC0003DMG400019604 PGSC0003DMG400027196 PGSC0003DMG400030332	上调UP
生长素转运蛋白 Auxin transporter- like protein 3	PGSC0003DMG400006550	UP上调
ABF ABRE binding factor	PGSC0003DMG400008011	UP上调
脱落酸受体 Abscisic acid receptor protein	PGSC0003DMG400011033 PGSC0003DMG400015897 PGSC0003DMG400023949	DOWN下调
脱落酸-不敏感蛋白 ABSCISIC ACID- INSENSITIVE 5- like protein 4	PGSC0003DMG400025889	UP上调
生长素应答蛋白 Auxin-responsive protein	PGSC0003DMG400026010 PGSC0003DMG402002635	UP上调 UP上调
吲哚-3-乙酸酰胺合成酶 Indole-3-acetic acid-amido synthetase	PGSC0003DMG401018368 PGSC0003DMG402018368	DOWN下调 DOWN下调
MYC4转录因子 Transcription factor MYC4	PGSC0003DMG400005525 PGSC0003DMG401010822 PGSC0003DMG402010822	DOWN下调

不同马铃薯耐盐性转录组比较及耐盐基因的挖掘

Comparative transcriptome analysis between two potato varieties with different salt-tolerance and further identification of potato salt-tolerance genes

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参考文献 29

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Metrics

名称 Name	基因ID Gene ID	表达模式 Expression pattern
查尔酮合成酶 Chalcone synthase G	PGSC0003DMG400008633 PGSC0003DMG400019110 PGSC0003DMG400029620	UP上调 UP上调 DOWN下调
乙酰辅酶A苄醇乙酰转移酶 Acetyl-CoA-benzylalcohol acetyltransferase-like	PGSC0003DMG400029256 PGSC0003DMG400029262	DOWN下调
黄烷酮3-羟化酶 Flavanone 3-hydroxylase	PGSC0003DMG400003563 PGSC0003DMG400011929	DOWN下调 UP上调
ECERIFERUM 1 蛋白 protein ECERIFERUM 1	PGSC0003DMG400002049	DOWN下调

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