早熟陆地棉种萌发中种胚保护性酶活性对盐胁迫的响应

doi:10.6048/j.issn.1001-4330.2023.01.001

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (1): 1-10.DOI: 10.6048/j.issn.1001-4330.2023.01.001

• 作物遗传育种 · 耕作栽培 · 种质资源 · 生理生化 • 上一篇下一篇

早熟陆地棉种萌发中种胚保护性酶活性对盐胁迫的响应

张红霞¹(), 雍晓宇¹, 何飞¹, 夏军¹, 李慧琴², 王潭刚²()

1.石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室,新疆石河子 832003
2.新疆生产建设兵团第三师农业科学研究所,新疆图木舒克 843901

收稿日期:2022-04-23 出版日期:2023-01-20 发布日期:2023-03-07
通信作者: 王潭刚(1984-),男,甘肃平凉人,副研究员,研究方向为棉花栽培,(E-mail)381762798@qq.com
作者简介:张红霞(1995-),女,甘肃通渭人,硕士研究生,研究方向为作物高产栽培, (E-mail) 2635172750@qq.com
基金资助:
国家棉花产业技术体系叶尔羌河综合试验站(CARS-15-50)

Effects of Different Concentrations of NaCl Stress on Seed Embryo Protective Enzyme Activity during Cotton Germination

ZHANG Hongxia¹(), YONG Xiaoyu¹, HE Fei¹, XIA Jun¹, LI Huiqin², WANG Tangang²()

1. Key Laboratory of Oasis Eco-Agriculture of Xinjiang Production and Construction Corps / College of Agronomy, Shihezi University, Shihezi Xinjiang 832003, China
2. Agricultural Science Institute of the Third Division of Xinjiang Production and Construction Corps, Tumushuke Xinjiang 843901, China

Received:2022-04-23 Published:2023-01-20 Online:2023-03-07
Correspondence author: WANG Tangang (1984 -), male, associate researcher, research direction is cotton cultivation technology, (E-mail)381762798@qq.com
Supported by:
China Agriculture Research System(CARS-15-50)

摘要/Abstract

摘要：

【目的】研究不同浓度盐胁迫对早熟棉花种子萌发特性及关键酶活性的影响。【方法】以新陆早45号和新陆早65号为试材,设置不同浓度的盐胁迫处理,测定棉种萌发过程中种胚相对含水量、发芽势、发芽率以及脯氨酸(Pro)、可溶性蛋白(SP)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)含量的变化。【结果】随盐胁迫处理时间的延长,0~15 h内相对含水量持续上升,15~60 h逐渐平缓,63 h时相对含水量有上升趋势。新陆早45号种子的相对含水量在不同盐浓度下表现为:0.2%比对照的相对含水量高,0.8%相对含水量最低;新陆早65号的不同浓度处理间无明显差异。吸水速率新陆早65号>新陆早45号,且相对含水量新陆早65号>新陆早45号;随盐浓度的增加,2个品种的发芽势均有下降;处理前60 h在0.2%、0.4%、0.6%和0.8%盐浓度处理下,新陆早65号的平均超氧化物歧化酶(SOD)活性较新陆早45号分别高出15%、19%、2%和13%,相同NaCl浓度胁迫的80 h内,新陆早65号过氧化氢酶(CAT)活性、游离脯氨酸(Pro)含量在0.2%、0.4%、0.6%和0.8%NaCl浓度下均比新陆早45号高,且均呈先增后减的趋势,可溶性蛋白(SP)呈增加趋势;相同浓度NaCl胁迫下,新陆早45号种子丙二醛含量均高于新陆早65号,且随NaCl浓度的增加丙二醛含量逐渐增加。新陆早45号和新陆早65号的SOD、CAT的载荷值较高。耐盐性新陆早65号>新陆早45号。【结论】盐胁迫下棉种可通过提高种胚超氧化物歧化酶(SOD)、过氧化氢酶(CAT)酶活性和降低丙二醛(MDA)含量来提高耐盐性,减轻盐胁迫对其出苗的伤害。

关键词: 早熟陆地棉; 盐胁迫; 萌发; 酶活性

Abstract:

【Objective】 To study the effects of different concentrations of NaCl stress on germination characteristics and key enzyme activities of cotton seeds. 【Method】 Xinluzao 45 and Xinluzao 65 were used as the testing materials and different concentrations of NaCl stress were set to determine relative water content, germination potential, germination rate, proline(Pro), soluble protein(SP), superoxide dismutase, (SOD), catalase (CAT), malonaldehyde(MDA). 【Result】 With the prolongation of the salt stress treatment time, the relative water content continued to increase within 0-15 h, gradually flattened from 15-60 h, and the relative water content showed an upward trend at 63 h.The relative water content of Xinluzao 45 seeds under different NaCl concentrations showed that: 0.2% was higher than the control relative water content, and 0.8% relative water content was the lowest; there was no significant difference between the different concentration treatments of Xinluzao 65.Among the varieties, Xinluzao 65 had a higher water absorption rate than Xinluzao 45, and the relative water content was: Xinluzao 65 > Xinluzao 45. 【Conclusion】 Salt stress in a certain range makes cotton seeds improve their salt tolerance by increasing SOD and CAT activities and reducing MDA content, thus reducing the damage of salt stress on seedling emergence.

Key words: cotton; salt stress; germination; enzymatic activity

中图分类号:

S562

张红霞, 雍晓宇, 何飞, 夏军, 李慧琴, 王潭刚. 早熟陆地棉种萌发中种胚保护性酶活性对盐胁迫的响应[J]. 新疆农业科学, 2023, 60(1): 1-10.

ZHANG Hongxia, YONG Xiaoyu, HE Fei, XIA Jun, LI Huiqin, WANG Tangang. Effects of Different Concentrations of NaCl Stress on Seed Embryo Protective Enzyme Activity during Cotton Germination[J]. Xinjiang Agricultural Sciences, 2023, 60(1): 1-10.

图/表 11

图1 不同浓度NaCl胁迫下2棉花品种相对含水量变化

Fig.1 Relative water content of two cotton varieties under different concentrations of NaCl stress

图2 不同NaCl胁迫下2棉花品种的相对发芽率、相对发芽势变化

Fig.2 Comparison between the relative germination rate and the relative germination potential of the two cotton varieties under different concentrations of NaCl stress

图3 不同NaCl胁迫下2棉花品种发芽指数比较

Fig.3 Comparison between germination indexes of the two cotton varieties under different concentrations of NaCl stress

表1 不同棉花品种盐害指数和盐害级别

Table 1 Salt damage index and salt harm level of different cotton varieties

NaCl浓度 NaCl concen tration (%)	盐害指数 Salt damage index		盐害级别 Salt damage level
NaCl浓度 NaCl concen tration (%)	新陆早45号 Xinlu zao 45	新陆早65号 Xinlu zao 65	新陆早45号 Xinlu zao 45	新陆早65号 Xinlu zao 65
0.2 0.4 0.6 0.8	0.26 0.40 0.46 0.63	0 0 0 0.3	2 2 3 4	无盐害无盐害无盐害 2

图4 不同NaCl胁迫下棉花种子脯氨酸(Pro)含量变化

Fig.4 Effects of different concentrations of NaCl stress on proline (Pro) content in cottonseeds

图5 不同NaCl胁迫下棉花种子可溶性蛋白含量变化(80 h)

Fig.5 Effects of different concentrations of NaCl stress on soluble protein content in cottonseeds(80 h)

图6 不同NaCl胁迫下棉花种子丙二醛(MDA)含量变化(80 h)

Fig.6 Effects of different concentrations of NaCl stress on alpodialdehyde (MDA) content in cottonseeds(80 h)

图7 不同NaCl胁迫下棉花种子超氧化物歧化酶(SOD)活性变化

Fig.7 Effects of different concentrations of NaCl stress on superoxide dismutase (SOD) content in cottonseeds

图8 不同浓度NaCl胁迫下棉种过氧化氢酶(CAT)活性变化

Fig.8 Effects of different concentrations of NaCl stress on catalase (CAT) content in cottonseeds

图9 盐胁迫下棉花发芽参数的主成分变异

Fig.9 Principal component analysis of NaCl stress and effects on germination parameters in cotton

表2 参数矩阵

Table 2 Parameter matrix analysis

棉花品种 Cotton varieties	新陆早45号 Xinluzao 45		新陆早65号 Xinluzao 65
参数 Paramter	提取成分 Extracted component		提取成分 Extracted component
参数 Paramter	PC₁	PC₂	PC₁	PC₂
SP	-0.559 57	0.086 63	-0.512 07	0.318 25
Pro	-0.501 11	0.439 58	0.411 08	-0.260 71
SOD	0.339 83	0.595 14	0.431 93	0.602 95
CAT	0.310 07	0.613 75	0.456 78	0.472 09
MDA	0.473 43	-0.261 5	-0.416 64	0.494 29

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早熟陆地棉种萌发中种胚保护性酶活性对盐胁迫的响应

Effects of Different Concentrations of NaCl Stress on Seed Embryo Protective Enzyme Activity during Cotton Germination

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