富氢水对干旱胁迫下大麦种子萌发的影响

doi:10.6048/j.issn.1001-4330.2022.01.010

摘要/Abstract

摘要：

【目的】研究富氢水对干旱胁迫下大麦种子萌发的影响,分析富氢水在干旱应答中的作用。【方法】以大麦品种新啤6号为试材,采用不同浓度(0、25%、50%、75%、100%)富氢水浸种处理,测定半致死聚乙二醇-6000浓度下大麦种子发芽特性、渗透调节物质含量、抗氧化酶活性及丙二醛含量变化。【结果】25%及50%富氢水浸种能明显改善干旱胁迫下大麦种子萌发质量。富氢水处理下,种子发芽率、发芽势、发芽指数显著增加;可溶性糖、可溶性蛋白及游离脯氨酸含量均有不同程度提升;丙二醛积累量显著下降;超氧化物歧化酶、过氧化物酶、过氧化氢酶活性明显增强。【结论】适宜浓度的富氢水能够通过提升渗透调节能力和抗氧化能力的途径增强大麦种子对干旱胁迫的耐受性。

关键词: 富氢水; 干旱胁迫; 大麦(Hordeum Vulgare L.); 萌发

Abstract:

【Objective】 To study the effects of hydrogen-rich water (HRW) on barley seeds germination under drought stress and clarify the role of HRW in drought response. 【Method】 The changes of germination characteristics, osmotic regulation substance content, antioxidant enzyme activity and malondialdehyde content of barley seeds (Xinpi 6) under the concentration of semi-lethal polyethylene glycol-6000 were measured by soaking seeds with different concentrations (0,25%, 50%,75%,100%).【Result】 25% and 50% HRW soaking seeds could significantly improve the germination quality of barley seeds under drought stress. Under the above concentration of HRW treatment, the germination potential, germination rate and germination index increased significantly (P<0.05); the contents of soluble protein, soluble sugar and free proline increased to different degrees; the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) also increased to different levels and the expression of malondialdehyde (MDA) decreased significantly (P<0.05). 【Conclusion】 The appropriate concentration of HRW can enhance the tolerance of barley seeds to drought stress by enhancing the ability of osmotic regulation and antioxidation.

Key words: hydrogen-rich water; drought stress; barley(Hordeum Vulgare L.); germination

中图分类号:

S512.3

宋瑞娇, 冯彩军, 齐军仓. 富氢水对干旱胁迫下大麦种子萌发的影响[J]. 新疆农业科学, 2022, 59(1): 79-85.

SONG Ruijiao, FENG Caijun, QI Juncang. Effects of Hydrogen-Rich Water on Barley Seed Germination under Drought Stress[J]. Xinjiang Agricultural Sciences, 2022, 59(1): 79-85.

图/表 4

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富氢水浓度 HRW level (%)	发芽率 Germination rate(%)	发芽势 Germination potential(%)	发芽指数 Germination index
0(CK)	60.0±2.0^c	34.0±1.2^b	52.51±0.16^c
25	73.3±0.7^a	49.3±4.1^a	75.72±4.08^a
50	69.3±0.7^b	47.3±4.4^a	70.48±3.81^a
75	67.3±0.7^b	34.0±1.2^b	60.23±0.26^b
100	62.7±1.3^c	28.0±2.3^b	51.92±0.69^c

富氢水浓度 HRW level (%)	发芽率 Germination rate(%)	发芽势 Germination potential(%)	发芽指数 Germination index
0(CK)	60.0±2.0^c	34.0±1.2^b	52.51±0.16^c
25	73.3±0.7^a	49.3±4.1^a	75.72±4.08^a
50	69.3±0.7^b	47.3±4.4^a	70.48±3.81^a
75	67.3±0.7^b	34.0±1.2^b	60.23±0.26^b
100	62.7±1.3^c	28.0±2.3^b	51.92±0.69^c

富氢水浓度 HRW level (%)	可溶性糖含量 Soluble sugar content (mg/g FW)	可溶性蛋白含量 Soluble protein content (mg/g FW)	游离脯氨酸含量 Free proline content (μg/g FW)
0(CK)	206.12±1.069^c	39.03±1.78^c	486.90±44.59^b
25	280.67±2.972^a	45.51±1.12^ab	565.19±19.69^ab
50	271.04±1.281^a	46.85±2.03^a	645.39±23.72^a
75	224.46±5.351^b	40.85±2.45^bc	632.69±21.24^a
100	226.64±8.753^b	40.34±0.84^bc	511.51±39.86^b

富氢水浓度 HRW level (%)	可溶性糖含量 Soluble sugar content (mg/g FW)	可溶性蛋白含量 Soluble protein content (mg/g FW)	游离脯氨酸含量 Free proline content (μg/g FW)
0(CK)	206.12±1.069^c	39.03±1.78^c	486.90±44.59^b
25	280.67±2.972^a	45.51±1.12^ab	565.19±19.69^ab
50	271.04±1.281^a	46.85±2.03^a	645.39±23.72^a
75	224.46±5.351^b	40.85±2.45^bc	632.69±21.24^a
100	226.64±8.753^b	40.34±0.84^bc	511.51±39.86^b

富氢水浓度 HRW level (%)	丙二醛含量 MDA content [μmol/(g FW)]	超氧化物歧化酶活性 SOD activity [U/(gFW·min)]	过氧化物酶活性 POD activity [U/(gFW·min)]	过氧化氢酶活性 CAT activity [U/(gFW·min)]
0(CK)	4.77±0.04^a	229.33±10.00^b	36.36±1.69^c	212.95±12.63^d
25	4.08±0.07^b	343.23±18.28^a	46.45±2.66^ab	337.26±52.20^bc
50	4.37±0.21^b	290.17±3.04^ab	50.76±4.43^a	467.44±8.20^a
75	4.36±0.06^b	318.97±13.98^a	41.72±0.69^bc	404.35±44.21^ab
100	4.46±0.09^a	259.20±11.86^ab	40.58±0.91^bc	285.14±17.13^cd