新疆农业科学 ›› 2023, Vol. 60 ›› Issue (8): 1879-1886.DOI: 10.6048/j.issn.1001-4330.2023.08.008
• 作物遗传育种·种质资源·分子遗传学·土壤肥料 • 上一篇 下一篇
鞠乐1,2(), 齐军仓1(
), 陈培育2, 牛银亭2, 阴志刚2
收稿日期:
2022-11-20
出版日期:
2023-08-20
发布日期:
2023-08-14
通信作者:
齐军仓(1971-),男,陕西宝鸡人,教授,博士生导师,研究方向为大麦遗传育种与栽培,(E-mail)shzqjc@qq.com作者简介:
鞠乐(1987-),女,河南邓州人,助理研究员,研究方向为杂粮遗传育种与栽培,(E-mail)695112004@qq.com
基金资助:
JU Le1,2(), QI Juncang1(
), CHEN Peiyu2, NIU Yinting2, YIN Zhigang2
Received:
2022-11-20
Published:
2023-08-20
Online:
2023-08-14
Correspondence author:
QI Juncang (1971-), male, Han, born in Baoji, Shaanxi Province, professor, doctoral supervisor. Research interests: Genetic breeding and cultivation techniques of barley,(E-mail)shzqjc@qq.comSupported by:
摘要:
【目的】研究干旱胁迫对大麦种子萌发、幼苗生长及生理特性的影响,筛选出PEG处理液最佳浓度,为构建大麦种子萌发期抗旱性评价提供理论依据。【方法】采用不同分子量、不同浓度PEG溶液模拟干旱胁迫进行大麦种子发芽试验,测定发芽势、发芽率、根长、芽长、芽鞘长、苗干重、根干重、芽干重,以及SOD、CAT、POD活性及丙二醛、脯氨酸、可溶性蛋白、可溶性糖含量等指标。【结果】(1)随着PEG浓度增加,大麦种子发芽率差异不显著;而发芽势仅在20%PEG处理下与对照差异显著。(2)随着PEG浓度的增加,对大麦幼苗生长具有显著的抑制作用。(3)随着PEG浓度的增加,丙二醛、脯氨酸、可溶性糖含量以及SOD、POD活性均总体呈显著上升趋势,而CAT活性与可溶性蛋白含量变化与对照相比差异不显著。【结论】20%PEG 8 000是大麦种子萌发期比较理想的抗旱性鉴定浓度。
中图分类号:
鞠乐, 齐军仓, 陈培育, 牛银亭, 阴志刚. 干旱胁迫对大麦种子萌发、幼苗生长及生理特性的影响[J]. 新疆农业科学, 2023, 60(8): 1879-1886.
JU Le, QI Juncang, CHEN Peiyu, NIU Yinting, YIN Zhigang. Effects of drought stress on seed germination, seedling growth and physiological characteristics of barley[J]. Xinjiang Agricultural Sciences, 2023, 60(8): 1879-1886.
PEG 分子量 PEG molecular weight | PEG 浓度 PEG concen tration (W/V)(%) | 发芽势 Germin- ation poten tial (%) | 发芽率 Germin- ation rate (%) | 相对 发芽势 Relative germin- ation potential | 相对 发芽率 Relative germin- ation rate |
---|---|---|---|---|---|
4 000 | 0 | 90ab | 95ab | 1.00a | 1.00a |
5 | 89ab | 93abc | 0.99a | 0.97ab | |
10 | 87ab | 90abcd | 0.96a | 0.94ab | |
15 | 83ab | 91abcd | 0.93a | 0.96ab | |
20 | 47c | 82d | 0.52b | 0.86b | |
6 000 | 0 | 90ab | 95ab | 1.00a | 1.00a |
5 | 91a | 96ab | 1.02a | 1.01a | |
10 | 87ab | 96ab | 0.96a | 1.01a | |
15 | 81ab | 96ab | 0.90a | 1.01a | |
20 | 52c | 85cd | 0.58b | 0.89ab | |
8 000 | 0 | 90ab | 95ab | 1.00a | 1.00a |
5 | 79b | 87bcd | 0.88a | 0.92ab | |
10 | 81ab | 90abcd | 0.90a | 0.94ab | |
15 | 88ab | 95ab | 0.98a | 1.00a | |
20 | 81ab | 96ab | 0.90a | 1.01a | |
10 000 | 0 | 90ab | 95ab | 1.00a | 1.00a |
5 | 91ab | 93abc | 1.01a | 0.97ab | |
10 | 86ab | 91abcd | 0.96a | 0.95ab | |
15 | 89ab | 97a | 0.99a | 1.02a | |
20 | 41c | 91abcd | 0.45b | 0.95ab |
表1 干旱胁迫下大麦种子萌发变化
Tab.1 Effects of drought stress on seed germination of barley
PEG 分子量 PEG molecular weight | PEG 浓度 PEG concen tration (W/V)(%) | 发芽势 Germin- ation poten tial (%) | 发芽率 Germin- ation rate (%) | 相对 发芽势 Relative germin- ation potential | 相对 发芽率 Relative germin- ation rate |
---|---|---|---|---|---|
4 000 | 0 | 90ab | 95ab | 1.00a | 1.00a |
5 | 89ab | 93abc | 0.99a | 0.97ab | |
10 | 87ab | 90abcd | 0.96a | 0.94ab | |
15 | 83ab | 91abcd | 0.93a | 0.96ab | |
20 | 47c | 82d | 0.52b | 0.86b | |
6 000 | 0 | 90ab | 95ab | 1.00a | 1.00a |
5 | 91a | 96ab | 1.02a | 1.01a | |
10 | 87ab | 96ab | 0.96a | 1.01a | |
15 | 81ab | 96ab | 0.90a | 1.01a | |
20 | 52c | 85cd | 0.58b | 0.89ab | |
8 000 | 0 | 90ab | 95ab | 1.00a | 1.00a |
5 | 79b | 87bcd | 0.88a | 0.92ab | |
10 | 81ab | 90abcd | 0.90a | 0.94ab | |
15 | 88ab | 95ab | 0.98a | 1.00a | |
20 | 81ab | 96ab | 0.90a | 1.01a | |
10 000 | 0 | 90ab | 95ab | 1.00a | 1.00a |
5 | 91ab | 93abc | 1.01a | 0.97ab | |
10 | 86ab | 91abcd | 0.96a | 0.95ab | |
15 | 89ab | 97a | 0.99a | 1.02a | |
20 | 41c | 91abcd | 0.45b | 0.95ab |
PEG 分子量 PEG molecular weight | PEG浓度 PEG concen- tration (W/V)(%) | 根长 Root length (cm) | 芽长 Buds long (cm) | 芽鞘长 Bud sheath long(cm) |
---|---|---|---|---|
4 000 | 0 | 4.88efg | 6.51cd | 1.80i |
5 | 5.40cdef | 6.61c | 2.07h | |
10 | 4.73efg | 5.32e | 2.09gh | |
15 | 4.34fg | 4.17fg | 2.21efgh | |
20 | 3.00h | 2.53h | 2.11gh | |
6 000 | 0 | 4.88efg | 6.51cd | 1.80i |
5 | 6.84a | 6.47cd | 2.22defgh | |
10 | 6.15abcd | 5.64de | 2.36bcdef | |
15 | 5.11def | 3.64g | 2.18fgh | |
20 | 3.97gh | 2.75h | 2.12gh | |
8 000 | 0 | 4.88efg | 6.51cd | 1.80i |
5 | 6.30abc | 8.56ab | 2.26defgh | |
10 | 6.26abc | 7.85b | 2.33cdefg | |
15 | 6.74a | 6.51cd | 2.42bcde | |
20 | 5.63bcde | 4.77ef | 2.46bcd | |
10 000 | 0 | 4.88efg | 6.51cd | 1.80i |
5 | 6.49ab | 8.95a | 2.30cdefgh | |
10 | 7.08a | 8.12ab | 2.51bc | |
15 | 6.92a | 6.66c | 2.76a | |
20 | 5.22cdef | 4.80ef | 2.59ab |
表2 干旱胁迫下大麦幼苗生长变化
Tab.2 Effects of drought stress on barley seedling growth
PEG 分子量 PEG molecular weight | PEG浓度 PEG concen- tration (W/V)(%) | 根长 Root length (cm) | 芽长 Buds long (cm) | 芽鞘长 Bud sheath long(cm) |
---|---|---|---|---|
4 000 | 0 | 4.88efg | 6.51cd | 1.80i |
5 | 5.40cdef | 6.61c | 2.07h | |
10 | 4.73efg | 5.32e | 2.09gh | |
15 | 4.34fg | 4.17fg | 2.21efgh | |
20 | 3.00h | 2.53h | 2.11gh | |
6 000 | 0 | 4.88efg | 6.51cd | 1.80i |
5 | 6.84a | 6.47cd | 2.22defgh | |
10 | 6.15abcd | 5.64de | 2.36bcdef | |
15 | 5.11def | 3.64g | 2.18fgh | |
20 | 3.97gh | 2.75h | 2.12gh | |
8 000 | 0 | 4.88efg | 6.51cd | 1.80i |
5 | 6.30abc | 8.56ab | 2.26defgh | |
10 | 6.26abc | 7.85b | 2.33cdefg | |
15 | 6.74a | 6.51cd | 2.42bcde | |
20 | 5.63bcde | 4.77ef | 2.46bcd | |
10 000 | 0 | 4.88efg | 6.51cd | 1.80i |
5 | 6.49ab | 8.95a | 2.30cdefgh | |
10 | 7.08a | 8.12ab | 2.51bc | |
15 | 6.92a | 6.66c | 2.76a | |
20 | 5.22cdef | 4.80ef | 2.59ab |
PEG分子量 PEG molecular weight | PEG浓度 PEG concentration (W/V)(%) | 苗干重 Seedling dry weight (mg) | 根干重 Root dry weight (mg) | 芽干重 Shoot dry weight (mg) | 根冠比 Root shoot ratio | 物质转运速率 Material transport rate (%) |
---|---|---|---|---|---|---|
4 000 | 0 | 144.8bc | 56.6ef | 88.2abc | 0.64g | 42.86abc |
5 | 149.5ab | 68.1abcd | 81.4bcd | 0.84de | 41.82abcd | |
10 | 118.5de | 58.6de | 59.9efg | 0.98bcd | 34.61ef | |
15 | 106.4ef | 56.1ef | 50.3gh | 1.11ab | 28.39g | |
20 | 84.9fg | 45.7fg | 39.1h | 1.17a | 20.00i | |
6 000 | 0 | 144.8bc | 56.6ef | 88.2abc | 0.64g | 42.86abc |
5 | 157.2ab | 70.0abc | 87.3bc | 0.80ef | 40.58bcd | |
10 | 142.4bcd | 69.4abcd | 73.0cde | 0.95cd | 34.94ef | |
15 | 104.0efg | 53.9ef | 50.1gh | 1.08abc | 25.95gh | |
20 | 80.5g | 41.6g | 39.0h | 1.07abc | 20.94hi | |
8 000 | 0 | 144.8bc | 56.6ef | 88.2abc | 0.64g | 42.86abc |
5 | 171.6a | 68.4abcd | 103.2a | 0.66fg | 46.73a | |
10 | 162.4ab | 70.3abc | 92.1ab | 0.76efg | 41.57abcd | |
15 | 151.9ab | 76.1a | 75.8cd | 1.00bc | 36.80de | |
20 | 123.3cde | 63.9bcde | 59.4efg | 1.08abc | 30.52fg | |
10 000 | 0 | 144.8bc | 56.6ef | 88.2abc | 0.64g | 42.86abc |
5 | 155.3ab | 62.8cde | 92.4ab | 0.68fg | 43.68ab | |
10 | 154.8ab | 71.1abc | 83.7bcd | 0.85de | 43.32ab | |
15 | 144.9bc | 74.3ab | 70.7def | 1.05abc | 37.64cde | |
20 | 111.8e | 56.6ef | 55.2fg | 1.02abc | 28.50g |
表3 干旱胁迫下大麦种子幼苗干物质积累变化
Tab.3 Effects of drought stress on dry matter accumulation in barley seed seedlings
PEG分子量 PEG molecular weight | PEG浓度 PEG concentration (W/V)(%) | 苗干重 Seedling dry weight (mg) | 根干重 Root dry weight (mg) | 芽干重 Shoot dry weight (mg) | 根冠比 Root shoot ratio | 物质转运速率 Material transport rate (%) |
---|---|---|---|---|---|---|
4 000 | 0 | 144.8bc | 56.6ef | 88.2abc | 0.64g | 42.86abc |
5 | 149.5ab | 68.1abcd | 81.4bcd | 0.84de | 41.82abcd | |
10 | 118.5de | 58.6de | 59.9efg | 0.98bcd | 34.61ef | |
15 | 106.4ef | 56.1ef | 50.3gh | 1.11ab | 28.39g | |
20 | 84.9fg | 45.7fg | 39.1h | 1.17a | 20.00i | |
6 000 | 0 | 144.8bc | 56.6ef | 88.2abc | 0.64g | 42.86abc |
5 | 157.2ab | 70.0abc | 87.3bc | 0.80ef | 40.58bcd | |
10 | 142.4bcd | 69.4abcd | 73.0cde | 0.95cd | 34.94ef | |
15 | 104.0efg | 53.9ef | 50.1gh | 1.08abc | 25.95gh | |
20 | 80.5g | 41.6g | 39.0h | 1.07abc | 20.94hi | |
8 000 | 0 | 144.8bc | 56.6ef | 88.2abc | 0.64g | 42.86abc |
5 | 171.6a | 68.4abcd | 103.2a | 0.66fg | 46.73a | |
10 | 162.4ab | 70.3abc | 92.1ab | 0.76efg | 41.57abcd | |
15 | 151.9ab | 76.1a | 75.8cd | 1.00bc | 36.80de | |
20 | 123.3cde | 63.9bcde | 59.4efg | 1.08abc | 30.52fg | |
10 000 | 0 | 144.8bc | 56.6ef | 88.2abc | 0.64g | 42.86abc |
5 | 155.3ab | 62.8cde | 92.4ab | 0.68fg | 43.68ab | |
10 | 154.8ab | 71.1abc | 83.7bcd | 0.85de | 43.32ab | |
15 | 144.9bc | 74.3ab | 70.7def | 1.05abc | 37.64cde | |
20 | 111.8e | 56.6ef | 55.2fg | 1.02abc | 28.50g |
PEG分子量 PEG molecular weight | PEG浓度 PEG concentration (W/V)(%) | SOD活性 SOD activity (μ/(g·min FW)) | CAT活性 CAT activity (μ/(g·min FW)) | POD活性 POD activity (μ/(g·min FW)) |
---|---|---|---|---|
4 000 | 0 | 49.23efgh | 561.11abcd | 78.33i |
5 | 41.19k | 471.11def | 293.75efgh | |
10 | 46.46hij | 390.00fgh | 310.58defg | |
15 | 47.51ghi | 313.33gh | 223.42gh | |
20 | 44.49ijk | 261.11h | 391.75abcd | |
6 000 | 0 | 49.23efgh | 561.11abcd | 78.33i |
5 | 43.18jk | 500.00def | 348.58cdef | |
10 | 53.60bcd | 551.11abcde | 368.92bcde | |
15 | 51.44def | 510.00def | 355.67cdef | |
20 | 58.90a | 467.78def | 263.67fgh | |
8 000 | 0 | 49.23efgh | 561.11abcd | 78.33i |
5 | 51.82cde | 540.00bcde | 318.42def | |
10 | 53.94bcd | 670.00ab | 471.33a | |
15 | 56.40ab | 417.78efg | 292.58efgh | |
20 | 55.33abc | 408.89efg | 215.17h | |
10 000 | 0 | 49.23efgh | 561.11abcd | 78.33i |
5 | 31.71l | 513.33def | 217.25gh | |
10 | 43.50jk | 685.56a | 390.58abcd | |
15 | 48.09fghi | 664.44abc | 415.67abc | |
20 | 51.03defg | 526.67cdef | 461.67ab |
表4 干旱胁迫下大麦幼苗抗氧化酶活性变化
Tab.4 Changes of antioxidant enzyme activities in barley seedlings under drought stress
PEG分子量 PEG molecular weight | PEG浓度 PEG concentration (W/V)(%) | SOD活性 SOD activity (μ/(g·min FW)) | CAT活性 CAT activity (μ/(g·min FW)) | POD活性 POD activity (μ/(g·min FW)) |
---|---|---|---|---|
4 000 | 0 | 49.23efgh | 561.11abcd | 78.33i |
5 | 41.19k | 471.11def | 293.75efgh | |
10 | 46.46hij | 390.00fgh | 310.58defg | |
15 | 47.51ghi | 313.33gh | 223.42gh | |
20 | 44.49ijk | 261.11h | 391.75abcd | |
6 000 | 0 | 49.23efgh | 561.11abcd | 78.33i |
5 | 43.18jk | 500.00def | 348.58cdef | |
10 | 53.60bcd | 551.11abcde | 368.92bcde | |
15 | 51.44def | 510.00def | 355.67cdef | |
20 | 58.90a | 467.78def | 263.67fgh | |
8 000 | 0 | 49.23efgh | 561.11abcd | 78.33i |
5 | 51.82cde | 540.00bcde | 318.42def | |
10 | 53.94bcd | 670.00ab | 471.33a | |
15 | 56.40ab | 417.78efg | 292.58efgh | |
20 | 55.33abc | 408.89efg | 215.17h | |
10 000 | 0 | 49.23efgh | 561.11abcd | 78.33i |
5 | 31.71l | 513.33def | 217.25gh | |
10 | 43.50jk | 685.56a | 390.58abcd | |
15 | 48.09fghi | 664.44abc | 415.67abc | |
20 | 51.03defg | 526.67cdef | 461.67ab |
PEG分子量 PEG molecular weight | PEG浓度 PEG concentration (W/V)(%) | 丙二醛含量 Malondialdehyde content(μmol/g) | 脯氨酸含量 Proline content (μg/g) | 可溶性蛋白含量 Soluble protein content(mg/g) | 可溶性糖含量 Soluble sugar content(mg/g) |
---|---|---|---|---|---|
4 000 | 0 | 13.95l | 31.79kl | 11.68cdef | 14.03kl |
5 | 15.53k | 33.90kl | 10.34fg | 17.50i | |
10 | 25.48f | 55.55j | 11.15defg | 12.19l | |
15 | 23.90gh | 126.86h | 11.73cdef | 14.93jk | |
20 | 23.51h | 541.35a | 12.83abc | 28.65e | |
6 000 | 0 | 13.95l | 31.79kl | 11.68cdef | 14.03kl |
5 | 23.79gh | 28.95l | 9.99g | 13.83kl | |
10 | 32.56c | 60.36j | 11.89bcdef | 16.04ij | |
15 | 32.91c | 225.45f | 12.13bcde | 21.80h | |
20 | 30.80d | 365.26d | 12.38bcd | 25.90f | |
8 000 | 0 | 13.95l | 31.79kl | 11.68cdef | 14.03kl |
5 | 19.84j | 58.51j | 10.55efg | 24.15fg | |
10 | 22.56i | 110.17i | 12.51abcd | 30.28de | |
15 | 28.25e | 175.87g | 11.98bcde | 31.11d | |
20 | 34.81a | 413.63c | 13.03abc | 34.80c | |
10 000 | 0 | 13.95l | 31.79kl | 11.68cdef | 14.03kl |
5 | 20.27j | 38.17k | 11.77cdef | 21.78h | |
10 | 24.50g | 101.80i | 13.48ab | 23.26gh | |
15 | 34.00b | 294.82e | 11.89bcdef | 42.98b | |
20 | 34.39ab | 518.07b | 14.07a | 50.94a |
表5 干旱胁迫下大麦幼苗丙二醛和渗透调节物质含量变化
Tab.5 Changes of malondialdehyde and osmotic regulatory substances in barley seedlings under drought stress
PEG分子量 PEG molecular weight | PEG浓度 PEG concentration (W/V)(%) | 丙二醛含量 Malondialdehyde content(μmol/g) | 脯氨酸含量 Proline content (μg/g) | 可溶性蛋白含量 Soluble protein content(mg/g) | 可溶性糖含量 Soluble sugar content(mg/g) |
---|---|---|---|---|---|
4 000 | 0 | 13.95l | 31.79kl | 11.68cdef | 14.03kl |
5 | 15.53k | 33.90kl | 10.34fg | 17.50i | |
10 | 25.48f | 55.55j | 11.15defg | 12.19l | |
15 | 23.90gh | 126.86h | 11.73cdef | 14.93jk | |
20 | 23.51h | 541.35a | 12.83abc | 28.65e | |
6 000 | 0 | 13.95l | 31.79kl | 11.68cdef | 14.03kl |
5 | 23.79gh | 28.95l | 9.99g | 13.83kl | |
10 | 32.56c | 60.36j | 11.89bcdef | 16.04ij | |
15 | 32.91c | 225.45f | 12.13bcde | 21.80h | |
20 | 30.80d | 365.26d | 12.38bcd | 25.90f | |
8 000 | 0 | 13.95l | 31.79kl | 11.68cdef | 14.03kl |
5 | 19.84j | 58.51j | 10.55efg | 24.15fg | |
10 | 22.56i | 110.17i | 12.51abcd | 30.28de | |
15 | 28.25e | 175.87g | 11.98bcde | 31.11d | |
20 | 34.81a | 413.63c | 13.03abc | 34.80c | |
10 000 | 0 | 13.95l | 31.79kl | 11.68cdef | 14.03kl |
5 | 20.27j | 38.17k | 11.77cdef | 21.78h | |
10 | 24.50g | 101.80i | 13.48ab | 23.26gh | |
15 | 34.00b | 294.82e | 11.89bcdef | 42.98b | |
20 | 34.39ab | 518.07b | 14.07a | 50.94a |
PEG分子量 PEG molecular weight | PEG浓度 PEG concentr- ation (W/V)(%) | 抗旱系数Drought resistance coefficient | 均值 Mean value | |||||||
---|---|---|---|---|---|---|---|---|---|---|
发芽势 Germination potential | 发芽率 Germination rate | 根长 Root length | 芽长 Buds long | 芽鞘长 Bud sheath long | 根干重 Seedling dry weight | 芽干重 Shoot dry weight | 苗干重 Seedling dry weight | |||
0 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
5 | 0.99 | 0.97 | 1.11 | 1.02 | 1.15 | 1.20 | 0.92 | 1.03 | 1.05 | |
4 000 | 10 | 0.96 | 0.94 | 0.97 | 0.82 | 1.16 | 1.04 | 0.68 | 0.82 | 0.92 |
15 | 0.93 | 0.96 | 0.89 | 0.64 | 1.23 | 0.99 | 0.57 | 0.73 | 0.87 | |
20 | 0.52 | 0.86 | 0.62 | 0.39 | 1.17 | 0.81 | 0.44 | 0.59 | 0.67 | |
0 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
5 | 1.02 | 1.01 | 1.40 | 0.99 | 1.24 | 1.24 | 0.99 | 1.09 | 1.12 | |
6 000 | 10 | 0.96 | 1.01 | 1.26 | 0.87 | 1.31 | 1.23 | 0.83 | 0.98 | 1.06 |
15 | 0.90 | 1.01 | 1.05 | 0.56 | 1.21 | 0.95 | 0.57 | 0.72 | 0.87 | |
20 | 0.58 | 0.89 | 0.81 | 0.42 | 1.18 | 0.73 | 0.44 | 0.56 | 0.70 | |
0 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
5 | 0.88 | 0.92 | 1.29 | 1.32 | 1.25 | 1.21 | 1.17 | 1.19 | 1.15 | |
8 000 | 10 | 0.90 | 0.94 | 1.28 | 1.21 | 1.29 | 1.24 | 1.04 | 1.12 | 1.13 |
15 | 0.98 | 1.00 | 1.38 | 1.00 | 1.34 | 1.34 | 0.86 | 1.05 | 1.12 | |
20 | 0.90 | 1.01 | 1.15 | 0.73 | 1.37 | 1.13 | 0.67 | 0.85 | 0.98 | |
0 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
5 | 1.01 | 0.97 | 1.33 | 1.37 | 1.28 | 1.11 | 1.05 | 1.07 | 1.15 | |
10 000 | 10 | 0.96 | 0.95 | 1.45 | 1.25 | 1.40 | 1.26 | 0.95 | 1.07 | 1.16 |
15 | 0.99 | 1.02 | 1.42 | 1.02 | 1.53 | 1.31 | 0.80 | 1.00 | 1.14 | |
20 | 0.45 | 0.95 | 1.07 | 0.74 | 1.44 | 1.00 | 0.63 | 0.77 | 0.88 |
表6 大麦种子萌发期抗旱性评价
Tab.6 Evaluation of drought resistance of barley seed at germination stage
PEG分子量 PEG molecular weight | PEG浓度 PEG concentr- ation (W/V)(%) | 抗旱系数Drought resistance coefficient | 均值 Mean value | |||||||
---|---|---|---|---|---|---|---|---|---|---|
发芽势 Germination potential | 发芽率 Germination rate | 根长 Root length | 芽长 Buds long | 芽鞘长 Bud sheath long | 根干重 Seedling dry weight | 芽干重 Shoot dry weight | 苗干重 Seedling dry weight | |||
0 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
5 | 0.99 | 0.97 | 1.11 | 1.02 | 1.15 | 1.20 | 0.92 | 1.03 | 1.05 | |
4 000 | 10 | 0.96 | 0.94 | 0.97 | 0.82 | 1.16 | 1.04 | 0.68 | 0.82 | 0.92 |
15 | 0.93 | 0.96 | 0.89 | 0.64 | 1.23 | 0.99 | 0.57 | 0.73 | 0.87 | |
20 | 0.52 | 0.86 | 0.62 | 0.39 | 1.17 | 0.81 | 0.44 | 0.59 | 0.67 | |
0 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
5 | 1.02 | 1.01 | 1.40 | 0.99 | 1.24 | 1.24 | 0.99 | 1.09 | 1.12 | |
6 000 | 10 | 0.96 | 1.01 | 1.26 | 0.87 | 1.31 | 1.23 | 0.83 | 0.98 | 1.06 |
15 | 0.90 | 1.01 | 1.05 | 0.56 | 1.21 | 0.95 | 0.57 | 0.72 | 0.87 | |
20 | 0.58 | 0.89 | 0.81 | 0.42 | 1.18 | 0.73 | 0.44 | 0.56 | 0.70 | |
0 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
5 | 0.88 | 0.92 | 1.29 | 1.32 | 1.25 | 1.21 | 1.17 | 1.19 | 1.15 | |
8 000 | 10 | 0.90 | 0.94 | 1.28 | 1.21 | 1.29 | 1.24 | 1.04 | 1.12 | 1.13 |
15 | 0.98 | 1.00 | 1.38 | 1.00 | 1.34 | 1.34 | 0.86 | 1.05 | 1.12 | |
20 | 0.90 | 1.01 | 1.15 | 0.73 | 1.37 | 1.13 | 0.67 | 0.85 | 0.98 | |
0 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | |
5 | 1.01 | 0.97 | 1.33 | 1.37 | 1.28 | 1.11 | 1.05 | 1.07 | 1.15 | |
10 000 | 10 | 0.96 | 0.95 | 1.45 | 1.25 | 1.40 | 1.26 | 0.95 | 1.07 | 1.16 |
15 | 0.99 | 1.02 | 1.42 | 1.02 | 1.53 | 1.31 | 0.80 | 1.00 | 1.14 | |
20 | 0.45 | 0.95 | 1.07 | 0.74 | 1.44 | 1.00 | 0.63 | 0.77 | 0.88 |
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