补喂竹叶提取物对伊犁马挤奶期间心率变异性的影响

doi:10.6048/j.issn.1001-4330.2022.11.025

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (11): 2793-2799.DOI: 10.6048/j.issn.1001-4330.2022.11.025

• 马运动生理与健康养殖 • 上一篇下一篇

补喂竹叶提取物对伊犁马挤奶期间心率变异性的影响

刘婷¹^,²(), 姚新奎¹^,²^,³(), 孟军¹^,²^,³, 曾亚琦¹^,²^,³, 王川坤¹^,², 任万路¹^,², 布沙热木·阿不都卡地尔¹, 高婉婷¹

1.新疆农业大学动物科学学院,乌鲁木齐 830052
2.新疆农业大学马产业研究院,乌鲁木齐 830052
3.新疆马繁育与运动生理重点实验室,乌鲁木齐 830052

收稿日期:2022-01-11 出版日期:2022-11-20 发布日期:2022-12-28
通信作者: 姚新奎(1961-),男,新疆奎屯人,教授,博士生导师,研究方向为动物生产学,(E-mail)YXK61@126.com
作者简介:刘婷(1996-),女,新疆乌鲁木齐人,硕士研究生,研究方向为动物生产学,(E-mail)344034697@qq.com
基金资助:
自治区创新环境(人才、基地)建设专项(PT2220);新疆维吾尔自治区重大科技专项(2022A02007-1);新疆伊犁马产业集群项目(2022xjmlt-z-02)

Effects of Bamboo Leaf Extract on Heart Rate Variability of Yili Horses during Milking

LIU Ting¹^,²(), YAO Xinkui¹^,²^,³(), MENG Jun¹^,²^,³, ZENG Yaqi¹^,²^,³, WANG Chuankun¹^,², REN Wanlu¹^,², Busharemu Abudukadier¹, GAO Wanting¹

1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
2. Institute of Horse Industry, Xinjiang Agricultural University, Urumqi 830052, China
3. Xinjiang Key Laboratory of Equine Breeding and Exercise Physiology, Urumqi 830052, China

Received:2022-01-11 Published:2022-11-20 Online:2022-12-28
Supported by:
The Innovation Environment (Talent, Base) Construction Project of Xinjiang Uygur Autonomous Region(PT2220);Major science and technology projects of Xinjiang Uygur Autonomous Region(2022A02007-1);Xinjiang Yili Horse Industry Cluster Project(2022xjmlt-z-02)

摘要/Abstract

摘要：

【目的】研究补喂竹叶提取物后伊犁马挤奶期间心率变异性的变化,分析不同剂量和不同饲喂试验期对伊犁马挤奶应激的影响。【方法】选取24匹伊犁母马,其体型结构相似,胎次相近,进行为期60 d的补饲竹叶提取物,0~20 d为第1阶段,21~40 d为第2阶段,41~60 d为第3阶段。采集马匹挤奶期间心率变异性指标。将24匹伊犁马随机分为对照组0 g/d;试验Ⅰ组10 g/d;试验Ⅱ组20 g/d;试验Ⅲ组30 g/d四组,分析不同时期和剂量的HRV差异性。【结果】第3阶段SDT HR显著高于第1阶段(P<0.05);第2和第3阶段RMSSD均显著高于第1阶段(P<0.05);第3阶段SD₁显著高于第1阶段(P<0.05);第3阶段SD₂极显著高于第2阶段(P<0.01),且第2阶段又极显著高于第1阶段(P<0.01)。第3阶段VLF极显著高于第1和第2阶段(P<0.01);第3阶段的LF和HF两个指标均极显著高于第1阶段和第2阶段(P<0.01);第3阶段TP极显著高于第1阶段和第2阶段(P<0.01)。对照组Mean HR显著高于试验Ⅰ组和试验Ⅲ组(P<0.05);第Ⅱ组SD₂极显著高于第Ⅳ组(P<0.01),且显著高于对照组(P<0.05);对照组SD₂和VLF显著低于试验Ⅰ组(P<0.05);对照组和试验Ⅰ组LF显著高于试验Ⅲ组(P<0.05);试验Ⅰ组中HF、TP显著高于试验Ⅲ组(P<0.05);对照组LF/HF显著低于试验Ⅰ组(P>0.05)。【结论】伊犁马补喂竹叶提取物能有效降低马匹交感神经活动,减少其挤奶时的应激反应。

关键词: 伊犁马; 心率变异性; 竹叶提取物; 挤奶期间; 应激反应

Abstract:

【Objective】 To study the changes in heart rate variability (HRV) of Yili horses during milking after supplementary feeding with bamboo leaf extract, and explore the effects of different doses and different feeding periods on milking stress of Yili horses. 【Methods】 In this experiment, 24 Yili mares with similar body shape and structure and similar parity were selected and they were fed with bamboo leaf extract for 60 days. 0-20 days was taken as stage 1, 21-40 days was taken as stage 2, and 41-60 days was taken as stage 3. The indexes of HRV were collected during milking. The selected Yili horses were randomly divided into control group (0 g/d), group Ⅰ (10 g/d), group Ⅱ (20 g/d) and group Ⅲ (30 g/d). HRV differences in different periods and doses were analyzed. 【Results】 The results during different feeding test periods showed that SDT HR in stage 3 was significantly higher than that in stage 1 (P<0.05); RMSSD in stage 2 and 3 were significantly higher than that in stage 1 (P<0.05); SD₁ in stage 3 was significantly higher than that in stage 1 (P<0.05); SD₂ in stage 3 was significantly higher than that in stage 2 (P<0.01), and stage 2 was significantly higher than that in stage 1 (P<0.01). VLF in stage 3 was significantly higher than that in stage 1 and 2 (P<0.01); LF and HF in stage 3 were significantly higher than those in stage 1 and 2 (P<0.01); TP in stage 3 was significantly higher than that in stage 1 and 2 (P<0.01). The analysis results in different groups showed that the Mean HR in control group was significantly higher than that in test group I and the test group III (P<0.05); SD₂ in group Ⅱ was significantly higher than that in group Ⅳ (P<0.01) and significantly higher than that in control group (P<0.05); SD₂ and VLF in control group were significantly lower than those in test group I (P<0.05); LF in control group and test group Ⅰ was significantly higher than that in test group Ⅲ (P<0.05); HF and TP in group Ⅰ were significantly higher than those in group Ⅲ (P<0.05); LF/HF in control group was significantly lower than that in test group I (P>0.05). 【Conclusion】 Considering the HRV changes of horses, supplementary feeding with bamboo leaf extract to Yili horses can effectively reduce the sympathetic nerve activity and stress response of horses during milking under the test conditions.

Key words: Yili horse; heart rate variability; bamboo leaf extract; during milking; stress response

中图分类号:

S821.4

刘婷, 姚新奎, 孟军, 曾亚琦, 王川坤, 任万路, 布沙热木·阿不都卡地尔, 高婉婷. 补喂竹叶提取物对伊犁马挤奶期间心率变异性的影响[J]. 新疆农业科学, 2022, 59(11): 2793-2799.

LIU Ting, YAO Xinkui, MENG Jun, ZENG Yaqi, WANG Chuankun, REN Wanlu, Busharemu Abudukadier, GAO Wanting. Effects of Bamboo Leaf Extract on Heart Rate Variability of Yili Horses during Milking[J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2793-2799.

图/表 7

参考文献 23

[1]	徐虎, 黄庆国, 马卫国, 等. 基于HRV指标变化监测昆明犬的运动效果及工作表现[J]. 西北农业学报, 2019, 28(7): 1061-1067.
	XU Hu, HUANG Qingguo, MA Weiguo, et al. Monitoring of Exercise Effect and Performance of Kunming Canine Based on Changes of HRV Indicators[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2019, 28(7): 1061-1067.
[2]	栗明月, 焦梦荷, 蒋林树, 等. 竹叶黄酮的生理功能及其应用前景[J]. 中国农学通报, 2018, 34(32):144-149.
	LI Mingyue, JIAO Menghe, JIANG Shulin, et al. Physiological Functiongs and Application Prospect of Bamboo Leaf Flavonoids[J]. Chinese Agricultural Science Bulletin, 2018, 34(32):144-149.
[3]	陆柏益, 张英, 吴晓琴. 竹叶黄酮的抗氧化性及其心脑血管药理活性研究进展[J]. 林产化学与工业, 2005,(3):120-124.
	LU Boyi, ZHANG Ying, WU Xiaoqin, et al. Advances in Studies on Antioxidative Activity and Cardiocerebrovascular Pharmacology of Bamboo Leaf Flavonoids[J]. Chemistry and Industry of Forest Products, 2005,(3):120-124.
[4]	Cipryan L, Stejskal P, O Bartáková, et al. Autonomic Nervous System Observation Through to Use of Spectral Analysis of Heart Rate Variability in Ice Hockey Players[J]. Acta Universitatis Palackianae OlomucensisGymnica, 2007, 37(4):17-21.
[5]	Sumi K, Suzuki S, Matsubara M, et al. Heart rate variability during high‐intensity field exercise in female distance runners[J]. Scandinavian Journal of Medicine & Science in Sports, 2006, 16(5):314-320.
[6]	李义, 童津津, 栗明月, 等. 竹叶提取物对热应激奶牛泌乳性能及血清生化、抗氧化和免疫指标的影响[J]. 动物营养学报, 2021, 33(2):900-912.
	LI Yi, TONG Jinjing, LI Mingyue, et al. Effects of Bamboo Leaf Extract on Lactation Performance and Indexes of Biochemical, Antioxidant and Immune in Serum of Dairy Cows during Heat Stress[J]. Acta Zoonutrimenta Sinica, 2021, 33(2): 900-912.
[7]	Kinnunen S, Laukkanen R, Haldi J, et al. Heart rate variability in trotters during different training periods[J]. Equine Veterinary Journal Supplement, 2006,(36):214-217. PMID
[8]	CN1517096A.竹叶黄酮在制药中的应用[P].
	CN1517096A. Application of bamboo leaf flavonoids in pharmacy[P].
[9]	钱和, 韩婵, 刘利兵. 食品中化学添加剂的功能与风险控制[J]. 化学进展, 2009, 21(11):2424-2434.
	QIAN He, HAN Chan, LIU Libing. The Function and Risk Control of Chemical Additives in Foods[J]. Progress in Chemistry, 2009, 21(11):2424-2434.
[10]	何跃君, 岳永德. 竹叶提取物的有效成分及其应用研究进展[J]. 生物质化学工程, 2008, (3):31-38.
	HE Yuejun, YUE Yongde. A Review of the Effective Component and Applications of Extracts from Bamboo Leaves[J]. Biomass Chemical Engineering, 2008,(3):31-38.
[11]	张银生. 音乐对心率变异性影响的研究[D]. 南京: 南京农业大学, 2007.
	ZHANG Yinsheng. Effects of Music on Heart Rate Variability[D]. Nanjing: Nanjing Agricultural University, 2007.
[12]	戴海伦. 有训练者和无训练者安静状态与中等强度运动状态心率变异性特征研究[D]. 金华: 浙江师范大学, 2020.
	DAI Hailun. Study on the Characteristics of Heart Rate Variability of the Trained and the Untrained in the State of Quiet and Moderate Intensity Exercise[D]. Hangzhou: Zhejiang Normal University, 2020.
[13]	肖熠, 陈代文, 余冰, 等. 竹叶黄酮对断奶仔猪生长性能及生理生化指标的影响[A].中国畜牧兽医学会.中国畜牧兽医学会2013年学术年会论文集[C]. 2013.
	XIAO Yi, CHEN Daiwen, YU Bing, et al. Effects of Bamboo Leaf Flavonoids on Growth Performance and Physiological and Biochemical Indexes of Weaned Piglets[A]. Chinese Association of Animal Science and Veterinary Medicine. Chinese Association of Animal Science and Veterinary Medicine[C]. 2013.
[14]	刘德义, 贺绍君, 刘世清, 等. 大豆黄酮对热应激奶牛血细胞数和生理常数的影响[J]. 中国奶牛, 2013, (1):23-26.
	LIU Deyi, HE Shaojun, LIU Shiqing, et al. The Effects of Daidzein on the Routine Physical Parameters in Heat-stressed Dairy Cows[J]. China Dairy Cattle, 2013,(1):23-26.
[15]	Lili Z, Yizhen M, Jiajun P, et al. Bamboo leaf extract ameliorates cardiac fibrosis possibly via alleviating inflammation, oxidative stress and apoptosis[J]. Biomedicine & pharmacotherapy, 2017, (95):808.
[16]	Uetake K, Hurnik J F, Johnson L. Effect of music on voluntary approach of dairy cows to an automatic milking system[J]. Applied Animal Behaviour Science, 1997, 53(3):175-182. DOI URL
[17]	Wells D L, Hepper P G, Coleman D, et al. A note on the effect of olfactory stimulation on the behaviour and welfare of zoo-housed gorillas[J]. Applied Animal Behaviour Science, 2006, 106(1):155-160. DOI URL
[18]	Makoto I, Asami K, Chie K. Heart rate variability with repetitive exposure to music[J]. Biological psychology, 2005, 70(1):61-66. PMID
[19]	Junichiro H, Yusaku S, Akira Y, et al. Accuracy of assessment of cardiac vagal tone by heart rate variability in normal subjects[J]. Excerpta Medica, 1991, 67(2):199-204.
[20]	舒刚. 竹叶黄酮对肉鸡的生物效应及作用机理研究[D]. 成都: 四川农业大学, 2015.
	SHU Gang. Study on the biological effect and mechanism of bamboo leaf flavonoid in broilers[D]. Chengdou: Sichuan Agricultural University, 2015.
[21]	Piskorski J, Ellert J, Krauze T, et al. Testing heart rate asymmetry in long, nonstationary 24 hour RR-interval time series[J]. Physiological Measurement, 2019, 40(10):105001. DOI URL
[22]	严璘璘, 骆宏, 危静, 等. 心理压力的测量方法及新技术[J]. 应用心理学, 2019, 25(1):32-47.
	YAN Linlin, LUO Hong, WEI Jing, et al. The Measurements of Mental Stress and New Methodologies[J]. Chinese Journal of Applied Psychology, 2019, 25(1):32-47.
[23]	王京, 赵莉, 赵婷婷. 伊伐布雷定对老年心力衰竭患者心功能、心率变异性及NT-proBNP水平的影响[J]. 药物评价研究, 2020, 43(11):2250-2253.
	WANG Jing, ZHAO Li, ZHAO Tingting. Effects of ivabradine on heart function, heart rate variability and NT-proBNP levels in elderly patients with heart failure[J]. Drug Evaluation Research, 2020, 43(11):2250-2253.

	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial groupⅡ	试验Ⅲ组 Trial groupⅢ
马匹数量 The number of horses	6	6	6	6
补饲剂量 Supple mentary feeding dose(g/d)	0	10	20	30

	对照组 Control group	试验Ⅰ组 Trial groupⅠ	试验Ⅱ组 Trial groupⅡ	试验Ⅲ组 Trial groupⅢ
马匹数量 The number of horses	6	6	6	6
补饲剂量 Supple mentary feeding dose(g/d)	0	10	20	30

项目 Items	第1阶段 The first stage	第2阶段 The second stage	第3阶段 The third stage	标准误 SE	P值 P-value
项目 Items	第1阶段 The first stage	第2阶段 The second stage	第3阶段 The third stage	标准误 SE	Trt	Date	Trt×Date
相邻R-R间期平均值 Mean RR	1 113.08	1 065.28	1 098.19	88.44	0.897	0.969	0.989
平均心率Mean HR	60.27	57.87	56.09	2.89	0.076	0.522	0.226
标准心率STD HR	8.47^b	18.27^ab	31.52^a	4.84	0.772	0.093	0.556
R-R间隔均值标准差 RMSSD	65.45^b	174.99^a	254.17^a	25.29	0.302	0.034	0.149
相邻心跳时间间隔>50 ms的百分比 NN50	20.50	61.78	93.00	27.28	0.967	0.297	0.767
相邻间期差值>50 ms的百分比 pNN50	7.69	21.73	34.17	8.24	0.937	0.213	0.739

项目 Items	第1阶段 The first stage	第2阶段 The second stage	第3阶段 The third stage	标准误 SE	P值 P-value
项目 Items	第1阶段 The first stage	第2阶段 The second stage	第3阶段 The third stage	标准误 SE	Trt	Date	Trt×Date
相邻R-R间期平均值 Mean RR	1 113.08	1 065.28	1 098.19	88.44	0.897	0.969	0.989
平均心率Mean HR	60.27	57.87	56.09	2.89	0.076	0.522	0.226
标准心率STD HR	8.47^b	18.27^ab	31.52^a	4.84	0.772	0.093	0.556
R-R间隔均值标准差 RMSSD	65.45^b	174.99^a	254.17^a	25.29	0.302	0.034	0.149
相邻心跳时间间隔>50 ms的百分比 NN50	20.50	61.78	93.00	27.28	0.967	0.297	0.767
相邻间期差值>50 ms的百分比 pNN50	7.69	21.73	34.17	8.24	0.937	0.213	0.739

项目 Items	第1阶段 The first stage	第2阶段 The second stage	第3阶段 The third stage	标准误 SE	P值 P-value
项目 Items	第1阶段 The first stage	第2阶段 The second stage	第3阶段 The third stage	标准误 SE	Trt	Date	Trt×Date
极低频功率VLF	132.06^B	392.31^B	1 397.99^A	141.71	0.897	0.969	0.989
低频功率LF	438.66^B	3 903.00^B	17 220.69^A	929.33	0.076	0.522	0.226
高频功率HF	611.81^B	7 118.49^B	26 356.42^A	2 044.01	0.772	0.093	0.556
总频TP	1 184.68^B	11 605.25^B	39 732.99^A	2 797.92	0.302	0.034	0.149
低频功率与高频功率的比值LF/HF	0.79	0.58	0.43	0.14	0.967	0.297	0.767

补喂竹叶提取物对伊犁马挤奶期间心率变异性的影响

Effects of Bamboo Leaf Extract on Heart Rate Variability of Yili Horses during Milking

在线阅读

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 23

相关文章 15

编辑推荐

Metrics

[1]	常笑康, 曾亚琦, 孟军, 王建文, 张亚昂, 李林玲, 邓海峰, 郑文祥, 姚新奎, 周静. 耐力运动对伊犁马血液生理指标的影响[J]. 新疆农业科学, 2024, 61(3): 742-748.
[2]	刘晔, 王彤亮, 孟军, 曾亚琦, 王建文, 衡士兵, 王培明, 姚新奎, 樊志伟. 不同强度遛马对伊犁马900 m测试赛成绩及血气指标的影响[J]. 新疆农业科学, 2024, 61(3): 749-756.
[3]	杨利平, 王川坤, 尚婷婷, 曾亚琦, 王建文, 诺鲁甫, 马康伟, 姚新奎, 孟军. 调教训练对伊犁马2 000 m速步赛血气指标的影响[J]. 新疆农业科学, 2024, 61(3): 757-765.
[4]	张仕琦, 李晓斌, 张文杰, 韩明, 王世昌, 郑文祥, 欧阳文, 祁居中, 杨开伦. 基于LC/MS的伊犁马3 600 m速度赛赛前、赛后血浆代谢组学差异变化[J]. 新疆农业科学, 2023, 60(2): 501-510.
[5]	刘律, 李晓斌, 马艳, 杨昊, 张文杰, 鲁豪, 卫鑫岚, 高俊杰, 欧阳文, 杨开伦. 补喂胍基乙酸对运动训练伊犁马血浆胍基乙酸、肌酸浓度的影响[J]. 新疆农业科学, 2022, 59(9): 2267-2275.
[6]	王洁, 孟军, 曾亚琦, 王建文, 吐尔逊江·吾木尔艾力, 王川坤, 袁鑫鑫, 王彤亮, 姚新奎. 伊犁马PFKM基因表达及酶活性分析[J]. 新疆农业科学, 2022, 59(11): 2758-2764.
[7]	王建文, 丁玉泽, 李璐伶, 姚新奎, 曾亚琦, 闫睛, 任万路, 王川坤, 孟军. 伊犁马LPL基因多态性及其与肉质性状关联性分析[J]. 新疆农业科学, 2022, 59(11): 2765-2771.
[8]	吴睿宸, 姚新奎, 孟军, 曾亚琦, 王川坤, 阿拉法特·艾合买提, 努尔塔依·库尔班塔依. 补饲不同剂量竹叶提取物对泌乳期伊犁马血液生化指标的影响[J]. 新疆农业科学, 2022, 59(11): 2772-2778.
[9]	张雪敏, 姚新奎, 孟军, 曾亚琦, 王建文, 叶尔哈拿提·卡得尔汗, 哈丽斯汗·巴哈依丁. 母源补喂竹叶提取物对伊犁马幼驹血液生化指标的影响[J]. 新疆农业科学, 2022, 59(11): 2779-2785.
[10]	马兵强, 姚新奎, 孟军, 曾亚琦, 王建文, 王川坤, 阿皮代姆·图尔荪. 补喂不同水平竹叶提取物对泌乳期伊犁马产奶量及乳成分的影响[J]. 新疆农业科学, 2022, 59(11): 2786-2792.
[11]	钟浩杰, 姚新奎, 罗鹏辉, 孟军, 姚岳扬, 王川坤, 任万路. 基于代谢组学分析补喂发酵豆粕对伊犁马驹粪便代谢物的影响[J]. 新疆农业科学, 2022, 59(11): 2800-2807.
[12]	高晨霞, 孟军, 吕燕, 曾亚琦, 王川坤, 麦迪娜姆・布力布力, 胡泽旭, 艾力卡木・阿比来提, 姚新奎. 连续波中频电疗对伊犁马1 200 m速度赛后血气指标的影响[J]. 新疆农业科学, 2022, 59(11): 2808-2814.
[13]	吴金秋, 张冲, 孟军, 曾亚琦, 加娜尔・努尔顿, 王川坤, 袁鑫鑫, 王彤亮, 姚新奎. 伊犁马MCT1基因多态性与速度性能的关联性分析[J]. 新疆农业科学, 2022, 59(11): 2815-2821.
[14]	马超鑫, 阿迪娜·吾斯曼, 贾怡琪, 陈晓, 孟军, 曾亚琦, 王建文, 王川坤, 姚新奎. 不同赛道维护方式对1 600 m速度赛伊犁马步态特征和比赛成绩的影响[J]. 新疆农业科学, 2022, 59(11): 2822-2830.
[15]	倪秀琳, 孟军, 欧阳文, 王建文, 车矿兵, 热孜艳木・努尔艾, 赛尔达尔・毛拉吐尔迪, 祖丽胡玛尔・阿卜来提, 姚新奎. 不同音乐对伊犁马运动性能及心率变异性的影响[J]. 新疆农业科学, 2022, 59(11): 2831-2837.