茉莉酸甲酯对红地球葡萄采后贮藏品质和病害的影响

doi:10.6048/j.issn.1001-4330.2022.01.022

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (1): 190-198.DOI: 10.6048/j.issn.1001-4330.2022.01.022

• 园艺特产·植物保护·生理生化 • 上一篇下一篇

茉莉酸甲酯对红地球葡萄采后贮藏品质和病害的影响

郑素慧¹(), 何庆², 张健¹, 关军锋³, 秦南南², 杨怡靖铭², 刘雪艳², 吴斌¹()

1.新疆农业科学院农产品贮藏加工研究所,乌鲁木齐 830091
2.新疆农业大学食品科学与药学学院,乌鲁木齐 830052
3.河北省农林科学院遗传生理研究所,石家庄 050051

收稿日期:2020-08-19 出版日期:2022-01-20 发布日期:2022-02-18
通信作者: 吴斌(1973-),男,江苏人,研究员,博士,硕/博士生导师,研究方向为果品采后生物,(E-mail) wx@126.com
作者简介:郑素慧(1982-),女,新疆人,副研究员,硕士,研究方向为果蔬采后贮运保鲜,(E-mail) 94468969@qq.com
基金资助:
国家重点研发计划(2018YFD0401302);国家自然科学基金(31860460);新疆农业科学院青年基金(xjnkq-2016020)

Effects of Exogenous Methyl Jasmonate on Quality and Disease of Grapefruit

ZHENG Suhui¹(), HE Qing², ZHANG Jian¹, GUAN Junfeng³, QIN Nannan², YANG Yijingming², LIU Xueyan², WU Bin¹()

1. Institute of Agricultural Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China
3. Institute of Genetics and Physiology,Hebei Academy of Agricultwre and Forestry Sciences,Shijiazhuang 050051,China

Received:2020-08-19 Published:2022-01-20 Online:2022-02-18
Supported by:
National Key R & D Program Project(2018YFD0401302);National Natural Science Foundation of China(31860460);Youth Fund Project of Xinjiang Academy of Agricultural Sciences(xjnkq-2016020)

摘要/Abstract

摘要：

【目的】研究茉莉酸甲酯(MeJA)浸泡处理对葡萄采后病害的控制及贮藏品质的影响。【方法】以新疆红地球葡萄为试材,采用50、100和200 μmol/L茉莉酸甲酯(methyl jasmonate,MeJA)室温(25℃)浸泡处理葡萄,(0±1)℃贮藏,每隔10 d测定果实硬度、可溶性固形物含量、可滴定酸含量及腐烂率的变化;果实采后损伤接种Botrytis cinerea,统计病斑直径和发病率的变化,测定不同处理苯丙氨酸解氨酶(PAL)、几丁质酶(CHI)、β-1,3葡聚糖酶(GLU)和多酚氧化酶(PPO)活性变化。【结果】MeJA处理可延缓果实硬度的下降,维持葡萄果实中可溶性固形物和可滴定酸含量,能抑制果实的腐烂;MeJA处理能有效抑制Botrytis cinerea侵染造成灰霉病发病率上升和病斑直径的扩大,与单一接种病原菌和MeJA处理相比,先用MeJA处理后接种Botrytis cinerea葡萄果实展现出更强的抗病反应,PAL、CHI、GLU和PPO活性显著提高(P<0.05)。【结论】葡萄采后MeJA处理对于保持红地球葡萄贮期果实品质和灰霉病病害控制效果显著。

关键词: 茉莉酸甲酯; 红地球葡萄; 贮藏品质; 灰霉病

Abstract:

【Objective】 To explore the effects of exogenous methyl jasmonate (MeJA) soaking on postharvest disease control and postharvest storage quality of grape in the hope of finding safe and efficient grape preservation preparation and technique to replace traditional methods. 【Methods】 The grapes (Vitis vinifera L. cv. Red Globe) were immersed with 50, 100 and 200 μmol/L methyl jasmonate (MeJA) for 10 min at room temperature (25℃), then stored at (0±1)℃. Fruit firmness, soluble solid content, titratable acid content and decay rate were tested every ten days respectively. Grapes were inoculated with Botrytis cinerea by epidermis damaged method, the diameter changes and incidence of spots were counted after being inoculated with Botrytis cinerea in grapes,meanwhile, the activity changes of phenylalanine ammonia lyase (PAL), Chitinase(CHI), β-1,3-glucanase (GLU) and Polyphenol oxidase (PPO) in different treatments were determined. 【Result】 The results showed that MeJA treatment could delay the decrease of fruit firmness and inhibit the decay during the storage. Botrytis cinerea grape pre-treated with MeJA showed stronger and faster response to the challenge of pathogen inoculation compared with either treatments alone, and exhibited a significant increase in the activities of defense-related enzymes including PAL, CHI, GLU and PPO as well as total phenols content(P<0.05). 【Conclusion】 The study further clarified the significant effect of exogenous MeJA on maintaining grape quality and reducing gray mold in the post-harvest storage period of grape, and also provided theoretical support and laid the foundation for the further application of MeJA in fruit preservation.

Key words: methyl jasmonate; Vitis vinifera L. cv. Red Globe; storage quality; Botrytis cinerea

中图分类号:

S663.1

郑素慧, 何庆, 张健, 关军锋, 秦南南, 杨怡靖铭, 刘雪艳, 吴斌. 茉莉酸甲酯对红地球葡萄采后贮藏品质和病害的影响[J]. 新疆农业科学, 2022, 59(1): 190-198.

ZHENG Suhui, HE Qing, ZHANG Jian, GUAN Junfeng, QIN Nannan, YANG Yijingming, LIU Xueyan, WU Bin. Effects of Exogenous Methyl Jasmonate on Quality and Disease of Grapefruit[J]. Xinjiang Agricultural Sciences, 2022, 59(1): 190-198.

图/表 8

图1 不同MeJA处理下葡萄果实自然腐烂率变化注:不同小写字母表示处理间差异显著(P<0.05),下同

Fig.1 Effects of MeJA treatment at different concentrations on decay rate of grapes Note:Different lower case letters indicate significant difference at 0.05 level. The same as below.

图2 不同MeJA处理下红地球葡萄果实硬度变化

Fig.2 Effects of MeJA treatment at different concentrations on the hardness of grapes

图3 不同MeJA处理下红地球葡萄果实可溶性固形物变化注:(A)和可滴定酸(B)含量的影响

Fig.3 Effects of MeJA treatment at different concentrations on soluble solids content Note:(A) and titratable acid (B) content of grapes

图4 MeJA不同浓度处理下葡萄果实发病率(A)和病斑直径(B)变化(第6 d)

Fig.4 Effects of different MeJA concentrations treatment on grape incidence (A) and lesion diameter (B) (No.6 d)

图5 不同MeJA处理下葡萄果实PAL活性变化

Fig.5 Effects of MeJA treatment on PAL activity of grapes

图6 不同MeJA处理下葡萄果实CHI活性变化

Fig.6 Effects of MeJA treatment on CHI activity of grapes

图7 不同MeJA下葡萄果实GLU活性变化

Fig.7 Effects of MeJA treatment on GLU activity of grapes

图8 不同MeJA处理对葡萄果实PPO活性变化

Fig.8 Effects of MeJA treatment on PPO activity of grapes

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茉莉酸甲酯对红地球葡萄采后贮藏品质和病害的影响

Effects of Exogenous Methyl Jasmonate on Quality and Disease of Grapefruit

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