多异瓢虫成虫对日本通草蛉集团内捕食作用的分子检测技术

doi:10.6048/j.issn.1001-4330.2020.04.007

摘要/Abstract

摘要： 【目的】研究利用分子检测技术评价棉田多异瓢虫对日本通草蛉的集团内捕食作用。【方法】根据基因库 NCBI 中的日本通草蛉的线粒体细胞色素氧化酶 I(COI)的基因序列(登录号为 KY806757),设计日本通草蛉的特异引物,并测定特异引物的灵敏度。利用 DNA 检测技术,在室内以该引物检测喂食5粒日本通草蛉卵的多异瓢虫成虫中肠内靶标食物的衰变情况,估算多异瓢虫对日本通草蛉的半衰期。【结果】所设计引物只对日本通草蛉DNA具有扩增效果,对与其同域发生的其它害虫和天敌不具扩增作用,其扩增片段大小约为220 bp,其最低检出浓度为0.117 ng/μL。多异瓢虫成虫喂食日本通草蛉卵后,检出率随消化时间呈现逐渐下降的趋势,到16 h检出率为0,多异瓢虫成虫半衰期为8.32 h。【结论】利用该技术可定量评价多异瓢虫与日本通草蛉集团内竞争。

关键词: 日本通草蛉; 细胞色素氧化酶 I 基因; 多异瓢虫成虫; 中肠内容物; 集团内竞争

Abstract: 【Objective】 To study on intraguild predation by adult Hippodamia variegata (Coleoptera:Coccinellidae) on Chrysoperla nipponensis by using molecular gut content analysis.【Method】 Molecular analysis were used to detect the DNA of C. nipponensis in the gut contents of H. variegate. Primers for the C. nipponensis were designed according to cytochrome oxidase subunit I gene in Genbank (accession No.KY806757), and the sensitivity of this primer was tested. Moreover, feeding experiments were performed to determine how long prey DNA remained detectable in the guts of H. variegate.【Result】 Species-specific tests showed that the primers were only used to amplify the DNA of the lacewing in C. nipponensis, and had no effect on other pests and natural enemies occurring in the The specificity tests performed with the primers showed that the single band presented in C. nipponensis, and no amplification to other pest and natural enemy of same ecological system. Primer The band was about 220 bp. The sensitivity tests detected with the primers indicat ed that 0.117 ng/μL of prey DNA can could be detected. Feeding experiments showed the detectability of prey DNA declined with digestion time extended. The half-life for adult H. variegata was estimated to be 8.32 h.【Conclusion】 This method can be used to study quantificationally the IGP by H. variegata on C. nipponensis predators in the field.

Key words: Chrysoperla nipponensis; Cytochrome oxidase subunit I gene; adult Hippodamia variegata; gut content analysis; intraguild predation

中图分类号:

S562

王冬梅, 李彩虹, 刘建. 多异瓢虫成虫对日本通草蛉集团内捕食作用的分子检测技术[J]. 新疆农业科学, 2020, 57(4): 636-641.

WANG Dongmei, LI Caihong, LIU Jian. Study of Molecular Analysis of the Gut Contents of Hippodamia variegata as a Method for Detecting Intraguild Predation of Chrysoperla nipponensis[J]. Xinjiang Agricultural Sciences, 2020, 57(4): 636-641.

参考文献

[1]Hemptinne JL, Magro A, Saladin C, et al. Role of intraguild predation in aphidophagous guilds [J]. Journal of Applied Entomology, 2012, 136(3):161-170.
[2]Chen Y, Giles KL, Payton ME,et al. Identifying key cereal aphid predators by molecular gut analysis [J]. Molecular Ecology, 2000, 9(11):1887-1898.
[3]Symondson WOC. Molecular identification of prey in predator diets [J]. Molecular Ecology, 2002, 11(4):627-641.
[4]Cuthbertson AGS, Fleming CC, Murchie AK. Detection of Rhopalosiphumin sertum (apple-grassaphid) predation by the predatory mite Anystis baccarum using molecular gut analysis [J]. Agricultural and Forest Entomology, 2003, 5(3):219-225.
[5]Harwood AD, Obrycki JJ. Quantifying aphid predation rates of generalist predators in the field [J]. Europen Journal Entomology, 2005, 102(3): 335-350.
[6]Sheppard SK, Harwood JD. Advances in molecular ecology: tracking trophic links through predator-prey food-webs [J]. Functional Ecology, 2005, 19(5):751-762.
[7]J King RA, Read DS, Traugott M, et al. Molecular analysis of predation: a review of best practice for DNA-based approaches [J]. Molecular Ecology, 2008, 17(4): 947-963.
[8]宋新元,丛斌,钱海涛,等.大豆蚜捕食性天敌捕食行为的 COI 基因标记检测[J].中国农业科学,2008,41(9):2881-2888.
SONG Xinyuan, CONG Bin, QIAN Haitao, et al. Identification of the key predators of Aphis glycines Matsumura (Homoptera: Aphididae) using COI gene markers [J]. Scientia Agricultura Sinica, 2008, 41(9):2881-2888.
[9] Galimberti A, Mattia FD, Losa A, et al. DNA barcoding as a new tool for food traceability[J] .Food Research International, 2013, 50(1) :55-63.
[10] Gagnon AE, Doyon J, Heimpel GE, et al. Prey DNA detection success following digestion by intraguild predators: influence of prey and predator species [J]. Molecular Ecology Resources, 2011, 11(6):1022-1032.
[11] Thomas AP, Trotman J, Wheatley A, et al. Predation of native coccinellids by the invasive alien Harmonia axyridis (Coleoptera: Coccinellidae): detection in Britain by PCR-based gut analysis [J]. Insect Conservation and Diversity, 2013,6(1):20-27.
[12] Rondoni G, Athey KJ, Harwood JD, et al. Development and application of molecular gut-content analysis to detect aphid and coccinellid predation by Harmonia axyridis (Coleoptera: Coccinellidae) in Italy [J]. Insect Science,2015, 22: 719-730.
[13] Yang F, Wang Q, Wang DM, et al. Intraguild predation Among three common Coccinellids (Coleoptera: Coccinellidae) in China: detection using DNA-based gut-content analysis [J]. Environmental Entomology, 2016, 1-10.
[14] Harwood JD, Desneux N, Yoo HJS, Rowley DL, et al. Tracking the role of alternative prey in soybean aphid predation by Orius insidiosus: a molecular approach [J]. Molecular Ecology, 2007, 16: 4390-4400.
[15] Mullins CB, Giles KL, Ye CM, et al. Using PCR to detect Intraguild predation of Lysiphlebus testaceipes by coccinellids[J]. Southwestern Entomologist, 2011, 36(3):295-304.
[16] Ingels BR, Abei AL, Hautuer L, et al. Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intraguild predation by this species on aphidophagous predators other than coccinellids [J]. European Journal of Entomology, 2013, 110( 4):567-576.
[17] 阿力甫·那思尔,祖母拉提·阿布都热依木,热依曼·阿迪,等.多异瓢虫与七星瓢虫、大草蛉不同虫态间的集团内捕食[J].生态学报,2014,34(22):6560-6567.
Alip Naser,Zumulati Abuduroim,Reheman Ady,et al. Intraguild predation among predatory insects Hippodamia variegata,Coccinella septempunctata and Chrysopa pallens at different stages [J].Acta Ecologica Sinica,2014,34(22):6560-6567.
[18] Greenstone M H, Rowley D L, Weber D C, et al. Feeding mode and prey detectability half-lives in molecular gut-content analysis: an example with two predators of the Colorado potato beetle[J]. Bulletin of Entomological Research, 2007, 97(2):201-209.
[19] Gomez-Polo P , Alomar O , Casta é, Cristina, et al. Molecular assessment of predation by hoverflies (Diptera: Syrphidae) in Mediterranean lettuce crops[J]. Pest Management Science, 2015, 71(9):1219-1227.
[20] Shokralla S, Spall JL, Gibson JF, et al. Next-generation sequencing technologies for environmental DNA research [J]. Molecular Ecology, 2012, 21(8):1794-1805.
[21] Pompanon F, Deagle B E, Symondson W O C, et al. Who is eating what: diet assessment using next generation sequencing [J]. Molecular Ecology, 2012, 21(8):1931-1950.
[22] Weber D C, Lundgren J G. Assessing the trophic ecology of the Coccinellidae: Their roles as predators and as prey [J]. Biological Control, 2009, 51(2):199-214.
[23] 杨帆,王倩,陆宴辉,等.瓢虫的集团内捕食作用.中国生物防治学报,2014,30(2):253-259.
YANG Fan, WANG Qian, LU Yanhui, et al. Intraguild predation of Coccinellid species [J]. Chinese Journal of Biological Control, 2014,30(2): 253-259.

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	0	0	0	98

来源	本网站	其他网站

次数	18	80
比例	18%	82%

摘要

308

最新录用	在线预览	正式出版

0	0	308

来源	本网站	其他网站

次数	84	225
比例	27%	73%