肉羊胚胎移植成活的影响因素实例分析

doi:10.6048/j.issn.1001-4330.2017.11.022

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (11): 2138-2145.DOI: 10.6048/j.issn.1001-4330.2017.11.022

肉羊胚胎移植成活的影响因素实例分析

郭延华¹, 刘长彬¹, 万鹏程¹, 倪建宏¹, 王立民¹, 周平¹, 杨永林¹, 方志忠², 顾宏伟², 付炳哲³, 卢全胜³

1.省部共建绵羊遗传改良与健康养殖国家重点实验室/新疆农垦科学院畜牧兽医研究所,新疆石河子 832000;
2.克拉玛依肉羊研究所,新疆克拉玛依 834000;
3.新疆华牛天俊生物技术有限公司,新疆昌吉 831100

收稿日期:2017-07-24 出版日期:2017-11-20 发布日期:2017-12-06
作者简介:郭延华(1983-),男,新疆喀什人,助理研究员,硕士,研究方向为动物遗传育种与繁殖,(E-mail)guoyanhuatlr@sohu.com
基金资助:
兵团院士基金“绵羊高效MOET生产及受体处理程序优化研究”(2013BB023);兵团科技攻关与成果转化计划项目“新品种选育与种质资源创新(超细毛羊)”(2016AC027);国家自然科学基金“基于羔羊体外成熟卵母细胞转录组分析及核质成熟相关机制研究”(31160460)

Case Study on the Factors Influencing the Survival of Mutton Sheep Embryo Transfer

GUO Yan-hua¹, LIU Chang-bin¹, WAN Peng-cheng¹, NI Jian-hong¹, WANG Li-min¹, ZHOU Ping¹, YANG Yong-lin¹, FANG Zhi-zhong², GU Hong-wei², FU Bing-zhe³, LU Quan-sheng³

1.Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences / State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Shihezi Xinjiang 832000 China;
2.The Sheep Research Institute of Karamay, Karamay Xinjiang 834000, China;
3.Xinjiang Huaniu Tianjun Livestock Breeding Engineering Co. Ltd., Changji Xinjiang 831100, China

Received:2017-07-24 Published:2017-11-20 Online:2017-12-06
Supported by:
The Academician fund of Xinjiang Production and Construction Corps "Optimization of high efficiency MOET production and receptor processing in sheep" (2013BB023); Programs of tackling key scientific problems and achievements transformation of XPCC "Breeding of new varieties and innovation of germplasm resources (superfine wool sheep)" (2016AC027) ; National Natural Scientific Foundation of China "Study on the transcriptome analysis and cytoplasmic maturation of lamb mature oocytes in vitro" (31160460)

摘要/Abstract

摘要： 【目的】胚胎移植技术是获得优秀种质资源的一种重要应用技术,研究影响胚胎存活和妊娠的因素。【方法】回顾性调查分析影响胚胎移植后成活的胚胎和受体因素。【结果】三种胚胎运输方式的妊娠率极显著低于就近移植的妊娠率(P＜0.01);胚胎卵裂期与囊胚期移植妊娠率差异不显著(P＞0.05),但以 2 细胞移植的妊娠率最低;鲜胚与冻胚的妊娠率差异不显著(P＞0.05),2 枚胚胎的妊娠率高于1枚胚胎的妊娠率,胚胎妊娠率随着胚胎质量而下降;囊胚与受体同期性差-24 h,妊娠率差异不显著(P＞0.05);放牧与舍饲的受体羊在胚胎妊娠率方面没有显著差异性(P＞0.05)。【结论】胚胎和受体因素对胚胎发生成功妊娠具有重要的影响。

关键词: 绵羊; 胚胎; 受体; 影响因素

Abstract: 【Objective】 Multiple ovulation and embryo transfer (MOET) technology is an important technique for obtaining excellent germplasm resources, but many factors affect embryo survival and pregnancy, and a large amount of data is needed to support it. The purpose of this study is to investigate the influence of embryonic and receptor factors on survival after embryo transfer.【Method】A retrospective survey about embryonic and recipient factors on the survival rate of transferred embryo was done in our application in mutton sheep.【Result】These results showed that the remote transition of donor, recipient and fresh embryo were adverse to gestation than on site transition (P＜0.01). There was no significant difference on pregnancy between embryonic cleavage and blastocyst stage(P＞0.05), but the rate of pregnancy with two cell transplantation on the embryonic cleavage stage was the lowest. And it was the same to embryo survival and recipient pregnancy rates after transfer of the fresh and vitrified in vitro produced ovine blastocysts (P＞0.05). The pregnancy rate of 2 embryos was higher than that of 1 embryo. The rate of embryo pregnancy decreased with the quality of embryo. The recipient pregnancy rate was not be affected by the blastocysts earlier than the synchronizing recipient on 24 hours after embryonic transfer (P＞0.05). There was no significant difference in the receptor pregnancy rate between the grazing and feeding sheep embryos (P＞0.05).【Conclusion】These results confirm that both embryonic and recipient factors play an important role in the success after embryo transfer in sheep.

Key words: sheep; embryo; recipient; factors

中图分类号:

S826.8

郭延华, 刘长彬, 万鹏程, 倪建宏, 王立民, 周平, 杨永林, 方志忠, 顾宏伟, 付炳哲, 卢全胜. 肉羊胚胎移植成活的影响因素实例分析[J]. 新疆农业科学, 2017, 54(11): 2138-2145.

GUO Yan-hua, LIU Chang-bin, WAN Peng-cheng, NI Jian-hong, WANG Li-min, ZHOU Ping, YANG Yong-lin, FANG Zhi-zhong, GU Hong-wei, FU Bing-zhe, LU Quan-sheng. Case Study on the Factors Influencing the Survival of Mutton Sheep Embryo Transfer[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2138-2145.

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肉羊胚胎移植成活的影响因素实例分析

Case Study on the Factors Influencing the Survival of Mutton Sheep Embryo Transfer

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