不同绵羊品种的产羔数候选基因遗传效应分析

doi:10.6048/j.issn.1001-4330.2024.06.029

摘要/Abstract

摘要：

【目的】研究不同绵羊品种产羔数候选基因的遗传效应。【方法】以4个绵羊品种(多浪羊468只、德国美利奴羊152只、萨福克羊157只、湖羊600只)为样本,利用扩增受阻突变体系PCR技术(ARMS-PCR)、限制性内切酶片段长度多态性技术(RFLP)等方法,检测4个繁殖力候选基因COIL、FSHR、GUCY1A1、BMPRIB遗传多态性,并分析其多态性与产羔数相关性。【结果】COIL基因g.7 321 466G>C位点在多浪、德国美利奴、萨福克、湖羊上突变纯合GG基因型频率在0.3左右,在多浪羊中CC基因型极显著高于CG、GG,CG基因型极显著高于GG;德国美利奴羊中GG基因型显著高于CG、CC,CG基因型显著高于GG;在萨福克、湖羊中GG、CG基因型极显著高于CC。BMPRIB基因在湖羊中BB、B+基因型产羔数极显著高于++,但其他品种效果不理想。FSHR基因g.75 320 741C>T位点、CUCY基因g.43266624G>A位点对绵羊产羔数影响较低。【结论】COIL基因g.7321466G>C位点有望替代BMPRIB基因成为新的产羔数性状选育的分子标记。

关键词: 绵羊; COIL; FSHR; GUCY1A1; BMPRIB; 产羔数

Abstract:

【Objective】 To explore candidate genes affecting lambing number in different sheep breeds. 【Methods】 Four sheep breeds (468 multi-wave sheep, 152 German merino sheep, 157 Suffolk sheep and 600 lake sheep) were used as samples, and the genetic polymorphisms of four fecundity candidate genes COIL, FSHR, GUCY1A1 and BMPRIB were detected by amplification hindered mutation system PCR technology (ARMS-PCR) and restriction enzyme fragment length polymorphism (RFLP), and the correlation between polymorphisms and lambing number was analyzed. 【Results】 The frequency of homozygous GG genotype in C-site of coil gene g.7321466G>C was about 0.3 in Duolang, German Merino, Suffolk and Hu sheep, CC genotype was significantly higher than that of CG and GG, and CG genotype was significantly higher than that of GG.The GG genotype in German merino sheep was significantly higher than that of CG and CC, and the CG genotype was significantly higher than that of GG.In Suffolk and Hu sheep, the GG and CG genotypes were significantly higher than those of CC.The association between BMPRIB gene genotype and lambing number in Hu sheep showed that the number of lambing of BB and B+ genotypes was significantly higher than that of ++, but the effect of other breeds was not satisfactory.The T locus of FSHR gene g.75320741C> and the g.43266624G>A locus of CUCY1A1 gene had low effects on lambing in sheep. 【Conclusion】 The g.7321466G>C locus of COIL gene is expected to replace BMPRIB gene as a molecular marker for the breeding of lamb number traits.

Key words: sheep; COIL; FSHR; GUCY1A1; BMPRIB; number of lambs

樊殊, 孙国智, 曹行, 史香云, 宋湘怡, 朱梦瑶, 刘玲玲, 刘武军. 不同绵羊品种的产羔数候选基因遗传效应分析[J]. 新疆农业科学, 2024, 61(6): 1544-1552.

FAN Shu, SUN Guozhi, CAO Hang, SHI Xiangyun, SONG Xiangyi, ZHU Mengyao, LIU Lingling, LIU Wujun. Analysis of genetic effects of candidate genes for lambing numbers in different sheep breeds[J]. Xinjiang Agricultural Sciences, 2024, 61(6): 1544-1552.

图/表 10

参考文献 21

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基因 Gene	位置 Position	引物序列 Primer Sequence
FSHR	75320741	F1: CTTCGCAAACTGGAGGCGGCACAT R1: GCTGGACAGGGAAGACTCACTCCCG
		F2: AAAATCTTAAGAAGCTGCGGGCCAAGTCA R2: AGCTGGGTAGCGCTCTCCTTGTGAACTC
COIL	7321466	F1: AGACTCAGACTCCGAGGAGGAATGGC
		R1: CATGGTCGTCCGTACAAAAGACAGACC
		F2: CTGGAAGATGATGTTCTGGAGGTCTTGC R2: AAATCGCCAAAGAAGAAGGAGAAACGTG
GUCY1A1	43266624	F1: AGACGCTCGGCTGCCCTGTGA R1: GGGAGTGGGCCAGCAGCGAC F2: GGCAGGCACTCACCTCTGGGAAAA R2: CCTGGACTCCCTGGTCACCAGCAT

基因 Gene	位置 Position	引物序列 Primer Sequence
FSHR	75320741	F1: CTTCGCAAACTGGAGGCGGCACAT R1: GCTGGACAGGGAAGACTCACTCCCG
		F2: AAAATCTTAAGAAGCTGCGGGCCAAGTCA R2: AGCTGGGTAGCGCTCTCCTTGTGAACTC
COIL	7321466	F1: AGACTCAGACTCCGAGGAGGAATGGC
		R1: CATGGTCGTCCGTACAAAAGACAGACC
		F2: CTGGAAGATGATGTTCTGGAGGTCTTGC R2: AAATCGCCAAAGAAGAAGGAGAAACGTG
GUCY1A1	43266624	F1: AGACGCTCGGCTGCCCTGTGA R1: GGGAGTGGGCCAGCAGCGAC F2: GGCAGGCACTCACCTCTGGGAAAA R2: CCTGGACTCCCTGGTCACCAGCAT

基因 Gene	基因型 Genotype	基因型频率Genotype frequency				等位基因 Alleles	等位基因频率 Allele frequency
基因 Gene	基因型 Genotype	多浪	德国美利奴	萨福克	湖羊	等位基因 Alleles	多浪	德国美利奴	萨福克	湖羊
Coil	CC	0.034	0.02	0.478	0.073	C	0.360	0.336	0.523	0.401
	CG	0.652	0.63	0.21	0.655	G	0.640	0.664	0.417	0.599
	GG	0.314	0.35	0.312	0.272
FSHR	CC	0.608	0.539	0.828	0.683	C	0.804	0.773	0.914	0.842
	CT	0.392	0.461	0.172	0.317	T	0.196	0.227	0.086	0.158
	TT	0	0	0	0
GUCY1A1	GG	0.797	0.796	0.783	0.92	G	0.896	0.898	0.892	0.96
	GA	0.203	0.204	0.217	0.08	A	0.104	0.102	0.108	0.04
	AA	0	0	0	0
BMPRIB	++	0.87	0.9	1	0.01	+	0.935	0.95	1	0.235
	B+	0.13	0.1	0	0.45	B	0.065	0.05	0	0.765
	BB	0	0	0	0.54

基因 Gene	基因型 Genotype	基因型频率Genotype frequency				等位基因 Alleles	等位基因频率 Allele frequency
基因 Gene	基因型 Genotype	多浪	德国美利奴	萨福克	湖羊	等位基因 Alleles	多浪	德国美利奴	萨福克	湖羊
Coil	CC	0.034	0.02	0.478	0.073	C	0.360	0.336	0.523	0.401
	CG	0.652	0.63	0.21	0.655	G	0.640	0.664	0.417	0.599
	GG	0.314	0.35	0.312	0.272
FSHR	CC	0.608	0.539	0.828	0.683	C	0.804	0.773	0.914	0.842
	CT	0.392	0.461	0.172	0.317	T	0.196	0.227	0.086	0.158
	TT	0	0	0	0
GUCY1A1	GG	0.797	0.796	0.783	0.92	G	0.896	0.898	0.892	0.96
	GA	0.203	0.204	0.217	0.08	A	0.104	0.102	0.108	0.04
	AA	0	0	0	0
BMPRIB	++	0.87	0.9	1	0.01	+	0.935	0.95	1	0.235
	B+	0.13	0.1	0	0.45	B	0.065	0.05	0	0.765
	BB	0	0	0	0.54

基因 Gene	品种 Varieties	P	纯合度 Ho	杂合度 He	有效等位基因数 Ne	多态性信息 PIC
Coil	多浪羊	<0.05	0.539	0.461	1.85	0.355
	德国美利奴羊	<0.05	0.554	0.446	1.805	0.346
	萨福克羊	<0.05	0.514	0.486	1.946	0.368
	湖羊	>0.05	0.520	0.480	1.924	0.365
FSHR	多浪羊	<0.05	0.685	0.316	1.46	0.265
	德国美利奴羊	<0.05	0.649	0.351	1.54	0.289
	萨福克羊	>0.05	0.843	0.157	1.187	0.145
	湖羊	<0.05	0.733	0.267	1.363	0.231
GUCY1A1	多浪羊	<0.05	0.817	0.182	1.233	0.166
	德国美利奴羊	>0.05	0.817	0.183	1.224	0.166
	萨福克羊	>0.05	0.807	0.193	1.239	0.174
	湖羊	>0.05	0.923	0.077	1.083	0.074
BMPRIB	多浪羊	>0.05	0.878	0.121	1.14	0.114
	德国美利奴羊	>0.05	0.906	0.094	1.1	0.09
	湖羊	<0.05	0.64	0.36	1.56	0.300