荨麻皮刺水提液对小鼠离体肠平滑肌运动的影响

doi:10.6048/j.issn.1001-4330.2022.03.027

摘要/Abstract

摘要：

【目的】研究荨麻促进消化道平滑肌运动的植株主要部位,分析其可能的受体机制。【方法】用生物信号处理系统监测麻叶荨麻皮刺与内茎(不含皮刺)水提液对小鼠离体小肠平滑肌的影响。【结果】2倍浓度递增(0.1%~3.2%)的皮刺剂量依赖性增加小肠收缩幅度、收缩张力和收缩峰值(P<0.05),但对收缩频率无影响(P>0.05);而内茎浓度达0.4%后使小肠收缩频率减慢(P<0.05)。0.8%皮刺一次性用药后小肠收缩幅度、收缩张力、收缩峰值均显著升高(P<0.05),而同剂量内茎对小肠收缩无影响(P>0.05)。M-受体阻断剂ATr与0.8%皮刺联合用药可使收缩幅度显著减弱(P<0.05)。【结论】荨麻植株的皮刺对消化道平滑肌具有促进作用,且可能部分作用是通过激活M-受体而实现。

关键词: 麻叶荨麻; 小鼠; 小肠; 平滑肌; M-受体; 皮刺

Abstract:

【Objective】 The purpose of this study aims to test the main parts of urtica simulating smooth muscle movement, and to explore its possible receptor mechanism.【Methods】 The biological signal processing system was used to monitor the effects of aqueous extract from urtica prickle or inner stem (without prickle) on the isolated small intestinal smooth muscle of mice.【Results】 A 2-fold increasing concentration (0.1% - 3.2%) of prickles increased the intestinal contract amplitude, tension and peak in a dose-dependent manner (P < 0.05), but had no effect on contract frequency (P > 0.05); When the concentration of inner stem reached 0.4%, the contract frequency of the small intestine was reduced (P < 0.05). The contract amplitude, tension, and peak of the small intestine were significantly increased (P < 0.05) after 0.8% prickle administration, but the same dose of inner stem had no effect on the small intestine contraction (P > 0.05). The combination of M-receptor blocker ATr and 0.8% prickle significantly reduced the contraction (P < 0.05).【Conclusion】 The prickle of urtica can promote the smooth muscle of the digestive tract, and the effect may be partly achieved by activating M-receptors.

Key words: Urtica cannabina; mouse; small intestine; smooth muscle; M-receptor; prickle

中图分类号:

S811.2

蔡鹏, 孙小燕, 阿迪莱·艾力, 雷艳, 单宝轩, 赵红琼. 荨麻皮刺水提液对小鼠离体肠平滑肌运动的影响[J]. 新疆农业科学, 2022, 59(3): 767-773.

CAI Peng, SUN Xiaoyan, Adilai Ali, LEI Yan, SHAN Baoxuan, ZHAO Hongqiong. Effects of Aqueous Extract of Urtica Prickle on the Motility of Isolated Intestinal Smooth Muscle in Mice[J]. Xinjiang Agricultural Sciences, 2022, 59(3): 767-773.

图/表 5

图1 不同KCl下小鼠离体肠平滑肌运动变化注:A:描记曲线,B:用药后3 min内统计数据;与用药前比较差异显著用*表示(P<0.05),差异极显著用#表示(P<0.01)

Fig.1 Effect of KCl on intestinal smooth muscle movement in mice Note: A:the tracing curve, B:the statistical results within 3 min after administration; the difference between the two groups is significantly expressed by* (P < 0.05), and the difference is extremely significant by# (P < 0.01)

图2 浓度递增下皮刺对小鼠离体肠平滑肌运动变化注:A:描记曲线,B:用药后10 s内统计数据;与用药前比较差异显著用*表示(P<0.05),差异极显著用#表示(P<0.01)

Fig.2 Effect of increasing concentration of prickly on intestinal smooth muscle movement of mice in vitro Note: A:the tracing curve, B:the statistical results within 10 s after administration; the difference between the two groups is significantly expressed by* (P<0.05), and the difference is extremely significant by# (P<0.01)

图3 浓度递增下内茎对小鼠离体肠平滑肌运动变化注:A:描记曲线,B:用药后10 s内统计数据,C-用药后3 min内统计数据;与用药前比较差异显著用*表示(P<0.05),差异极显著用#表示(P<0.01)

Fig.3 Effect of increasing prickly bark concentration on intestinal smooth muscle movement of mice in vitro Note: A:the tracing curve, B:the statistical results within 10 s after administration, C-the statistical results within 3 min after administration; the difference between the two groups is significantly expressed by* (P<0.05), and the difference is extremely significant by# (P<0.01)

图4 荨麻皮刺与内茎对小鼠离体肠平滑肌运动影响的比较注:A:皮刺描记曲线,B:内茎描记曲线,C:用药后10 s内统计数据;与用药前比较差异显著用*表示(P<0.05),差异极显著用**表示(P<0.01);皮刺与内茎之间比较差异显著用#表示(P<0.05),差异极显著用##表示(P<0.01)

Fig.4 Effect of urtica prickle and inner stem on intestinal smooth muscle movement of mice in vitro Note: A:the prick tracing curve, B:the inner stem tracing curve, C:the statistical data within 10 s after administration; the difference between a-prick and inner stem was significantly expressed by* (P<0.05), and the difference between a-prick and internal stem was significantly expressed by** (P<0.01); the difference between prick and internal stem was significantly expressed by# (P<0.05), and the difference was extremely significant expressed by## (P<0.01)

图5 荨麻皮刺与M-受体阻断剂联合用药下小鼠离体肠平滑肌运动变化注:A:描记曲线,B:用药后10 s内统计数据;与用药前比较差异显著用*表示(P<0.05),差异极显著用#表示(P<0.01)

Fig.5 Effect of urtica prickle combined with M-receptor blocker on intestinal smooth muscle movement of mice in vitro Note: A:the tracing curve, B:the statistical data within 10 s after administration; the difference between before administration and before administration was significantly indicated by* (P<0.05), and the difference was extremely significant indicated by# (P<0.01)

参考文献 18

[1]	杜明伟. 荨麻中植物多糖的提取分离及药理作用的研究[D]. 哈尔滨:哈尔滨商业大学, 2015.
	DU Mingwei. Extraction, Separation And Pharmacological Study Of Plant Polysaccharides From Urtica[D]. Harbin: Harbin Business University, 2015.
[2]	许长乐. 荨麻饲用性能及其毒副作用观察[J]. 新疆农垦科技, 1981, (6): 17-18.
	XU Changle. Observation on feeding performance and toxic and side effects of Urtica[J]. Xinjiang Farm Research of Science and Technology, 1981, (6): 17-18.
[3]	苏日娜. 藏药高原荨麻降尿酸、抗良性前列腺增生物质基础及作用机制研究[D]. 南昌:江西中医药大学, 2019.
	Surina . Study On The Material Basis And Mechanism Of Action Of Tibetan Medicine High Altitude Nettle In Lowering Uric Acid And Anti BPH[D]. Nanchang: Jiangxi University of Traditional Chinese Medicine, 2019.
[4]	谭麒麟, 陶恩, 李红玲, 等. 荨麻的药理作用研究进展[J]. 医学综述, 2018, 24(20): 4118-4122.
	TAN Qilin, TAO En, LI Hongling, et al. Research progress in the pharmacological action of Urtica[J]. Medical Review, 2018, 24(20): 4118-4122.
[5]	苏日娜, 罗维早, 朱继孝, 等. 荨麻属药用植物研究进展[J]. 中草药, 2018, 49(11): 2722-2728.
	SU Rina, LUO Weizao, ZHU Jixiao, et al. Research progress of Urtica medicinal plants[J]. Chinese Herbal Medicine, 2018, 49(11): 2722-2728.
[6]	李成义, 杨扶德, 林丽, 等. 麻叶荨麻的初步研究[C]// 中国中药学会全国第五届中药鉴定学术大会, 2001.
	LI Chengyi, YANG Fude, LIN Li, et al. A preliminary study on Urtica Sinica[C]. The Fifth National Congress of identification of traditional Chinese medicine, Chinese Academy of Traditional Chinese Medicine, 2001.
[7]	闫书平, 卡力比夏提·艾木拉江, 哈力旦·阿合买提, 等. 干鲜荨麻提取物对小鼠离体小肠运动影响的比较研究[J]. 动物医学进展, 2017, 38(9): 55-60.
	YAN Shuping, Kalibixiati , Aimulajiang , Halidan , et al. Comparative study on the effect of extract of fresh and dried nettle on the movement of small intestine in mice in vitro[J]. Advances in Animal Medicine, 2017, 38(9): 55-60.
[8]	闫书平. 荨麻对小鼠和羊消化道运动机能影响的研究[D]. 乌鲁木齐:新疆农业大学, 2017.
	YAN Shuping. Study on the effect of Urtica on the gastrointestinal motility of mice and sheep[D]. Urumqi: Xinjiang Agricultural University, 2017.
[9]	周玉东. 荨麻对鼠小肠和子宫运动影响的研究[D]. 乌鲁木齐:新疆农业大学, 2015.
	ZHOU Yudong. Study on the effect of nettle on the movement of small intestine and uterus in rats[D]. Urumqi: Xinjiang Agricultural University, 2015.
[10]	敖特根白音, 李运起, 韩艳华, 等. 国内外荨麻的研究进展[J]. 中国野生植物资源, 2015, 34(1): 32-36.
	Aotegen Baiyin, LI Yunqi, HAN Yanhua, et al. Research progress of nettle at home and abroad[J]. China Wild Plant Resources, 2015, 34(1): 32-36.
[11]	Maitai C K, Talalaj S, Njoroge D, et al. Effect of extract of hairs from the herb Urtica massaica, on smooth muscle[J]. Toxicon: Official Journal of the International Society on Toxinology, 1980, 18(2).
[12]	张丹. 苦豆子对鼠小肠和子宫运动影响的研究[D]. 乌鲁木齐:新疆农业大学, 2016.
	ZHANG Dan. Study on the effect of Sophora alopecuroides on the movement of small intestine and uterus in mice[D]. Urumqi: Xinjiang Agricultural University, 2016.
[13]	杨阳, 姚晓慧, 周玉东, 等. 辣椒、花椒和胡椒联合用药对小鼠离体小肠运动影响的研究[J]. 黑龙江畜牧兽医, 2016, (13): 197-200.
	YANG Yang, YAO Xiaohui, ZHOU Yudong, et al. Effect of co-administration of chili, wild pepper and black pepper on motility of the isolated Small intestine from mice[J]. Heilongjiang Animal Science and Veterinary Medicine, 2016,(13): 197-200.
[14]	刘悦秋, 孙向阳. 异株荨麻的活性成分及开发利用[J]. 国外医学(中医植物药学分册), 2005,(3): 144-148.
	LIU Yueqiu, SUN Xiangyang. Active components and development and utilization of different Urticas[J]. Foreign Medicine (Herbal Medicine of Traditional Chinese Medicine), 2005,(3): 144-148.
[15]	王梦月, 卫莹芳, 黄晓燕. 荨麻属药用植物研究进展[J]. 中药材, 2002, (1): 58-60.
	WANG Mengyue, WEI Yingfang, HUANG Xiaoyan. Research progress of Urtica medicinal plants[J]. Chinese Herbal Medicine, 2002,(1): 58-60.
[16]	李小平, 张菁华. 反相高效液相色谱法同时测定荨麻中绿原酸与咖啡酸[J]. 中国医药导报, 2012, 9(31): 112-114.
	LI Xiaoping, ZHANG Jinghua. Simultaneous determination of chlorogenic acid and caffeic acid in Urtica by RP-HPLC[J]. China Medical Herald, 2012, 9(31): 112-114.
[17]	张经济. 关于胃电的研究[J]. 生理科学进展, 1979,(4): 312-317.
	ZHANG Jingji. Research on gastric electricity[J]. Progress in Physiological Science, 1979, (4): 312-317.
[18]	刘荣珍. 阿托品药理作用以及在动物临床上的应用[J]. 中国畜牧兽医文摘, 2012, 28(10): 209.
	LIU Rongzhen. Pharmacological action of atropine and its clinical application in animals[J]. China Animal Husbandry and Veterinary Abstracts, 2012, 28(10): 209.

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