Analysis of the volatile aromatic components in fresh horse milk by headspace solid phase microextraction-Gas chromatography-Mass spectrometry

doi:10.6048/j.issn.1001-4330.2023.12.025

Abstract

Abstract:

【Objective】To compare and study the volatile aromatic components in fresh horse milk from different areas of Xinjiang, so as to provide scientific and technical support for the industrialization development and related research of Xinjiang horse milk and its products.【Methods】Fresh horse milk from Yili, Altay and Tacheng in northern Xinjiang was selected as the research object, and the volatile aromatic components in fresh horse milk were analyzed by headspace solid phase microextraction combined with gas chromatography-mass spectrometry.【Results】A total of 32 volatile aroma components were detected, including 21 aromatic components in fresh horse milk from Yili region, 17 aromatic components from Altay region and 21 aromatic components from Tacheng region. There were 11 volatile aroma components in the three regions, including three esters of ethyl octanoate, methyl octanoate and ethyl decanoate, five acids of octanoate, capric acid, caproic acid, nonanoic acid and lauric acid, and 1, 3-di-tert-butyl benzene, 2, 4-di-butyl phenol and dimethyl sulfone.【Conclusion】The volatile aromatic components in fresh horse milk are mainly esters and acids, and the main esters are methyl octanoate, ethyl octanoate and ethyl decanoate. The main acid components are octanoic acid and capric acid. The superposition, synergy and inhibition of these volatile aroma components make fresh horse milk have aroma different from cow milk and camel milk.

Key words: fresh mares milk; headspace solid phase micro -extraction (HS -SPME); gas chromatography-mass spectrometry (GC-MS); aroma components

摘要：

【目的】比较和研究新疆不同地区鲜马乳中的挥发性芳香成分,为新疆马乳及制品的产业化发展和相关研究提供科学依据。【方法】以新疆伊犁、阿勒泰、塔城三地的鲜马乳为研究对象,采用顶空固相微萃取-气相色谱-质谱联用技术分析新鲜马乳中挥发性芳香成分。【结果】在鲜马乳中检测出32种挥发性香气成分,伊犁地区鲜马乳中芳香成分21种,阿勒泰地区17种,塔城地区21种;三地共有挥发性香气成分11种,包括辛酸乙酯、辛酸甲酯、癸酸乙酯等3种酯类,辛酸、癸酸、己酸、壬酸、月桂酸这5种酸类,以及1,3-二叔丁基苯、2,4-二特丁基苯酚、二甲基砜。【结论】鲜马乳中的挥发性芳香成分均以酯类和酸类为主,酯类成分中辛酸甲酯、辛酸乙酯、癸酸乙酯为主;酸类成分以辛酸和癸酸为主。这些挥发性香气成分之间的叠加、协同、抑制等作用,使得鲜马乳具有区别于牛乳、驼乳等鲜乳的香气。

关键词: 鲜马乳, 顶空固相微萃取, 气质联用, 芳香成分

CLC Number:

S811.6

Bahatiguli Manatibai, YAO Weiqin, Gaoshaer Kayierhali, GUO Hengxin, Dulati Kayimaerdan, Ailati gemadi, SHI Bo, GONG Zhiguo. Analysis of the volatile aromatic components in fresh horse milk by headspace solid phase microextraction-Gas chromatography-Mass spectrometry[J]. Xinjiang Agricultural Sciences, 2023, 60(12): 3080-3085.

巴哈提古丽·马那提拜, 姚伟琴, 高沙尔·卡依尔哈力, 郭恒新, 杜拉提·卡衣马尔旦, 艾拉提·格玛迪, 史博, 巩志国. 采用顶空固相微萃取-气相色谱-质谱法检测及分析鲜马乳中挥发性芳香成分[J]. 新疆农业科学, 2023, 60(12): 3080-3085.

Figures/Tables 4

References 24

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化合物种类 Type of compound	化合物名称 Name of compound	相对百分含量 Relative content(%)
化合物种类 Type of compound	化合物名称 Name of compound	伊犁 Yili	阿勒泰 Aletai	塔城 Tacheng
酯类 Esters	丁酸乙酯	6.44	—	—
	己酸甲酯	0.90	—	1.87
	己酸乙酯	6.62	—	—
	辛酸甲酯	15.73	3.1	21.35
	辛酸乙酯	28.34	16.49	4.16
	癸酸乙酯	0.67	1.56	0.73
	癸酸甲酯	—	1.08	14.72
	甲酸甲酯	—	5.66	0.53
	4-癸烯酸甲酯	—	—	3.3.
	月癸酸甲酯	—	—	0.51
	辛乙烯二醇单正十二烷基酯	—	—	0.25
酸类 Acids	己酸	0.49	2.74	1.44
	辛酸	13.65	10.69	18.5
	壬酸	0.20	2.78	0.29
	癸酸	12.73	9.42	11.44
	9-癸烯酸	0.77	—	2.45
	月桂酸	0.75	2.27	2.93
	苯甲酸	—	4.22	—
	丁酸	—	—	0.75
其它 Other	甲苯	0.68	—	—
	2-庚酮	0.93	—	—
	3-甲基-1-丁醇	3.74	—	—
	苯乙烯	2.18	—	3.36
	2-壬酮	0.21	—	—
	E,E-2,4-庚二烯醛	0.36	—	—
	1,3-二叔丁基苯	3.74	10.46	9.78
	2,4-二特丁基苯酚	0.55	4.10	1.20
	二甲基砜	0.32	2.06	1.62
	2-乙基-1-己醇	—	20.30	—
	对甲苯酚	—	1.50	—
	3-甲基苯酚	—	1.57	—
	侧柏酮	—	—	2.12

化合物种类 Type of compound	化合物名称 Name of compound	相对百分含量 Relative content(%)
化合物种类 Type of compound	化合物名称 Name of compound	伊犁 Yili	阿勒泰 Aletai	塔城 Tacheng
酯类 Esters	丁酸乙酯	6.44	—	—
	己酸甲酯	0.90	—	1.87
	己酸乙酯	6.62	—	—
	辛酸甲酯	15.73	3.1	21.35
	辛酸乙酯	28.34	16.49	4.16
	癸酸乙酯	0.67	1.56	0.73
	癸酸甲酯	—	1.08	14.72
	甲酸甲酯	—	5.66	0.53
	4-癸烯酸甲酯	—	—	3.3.
	月癸酸甲酯	—	—	0.51
	辛乙烯二醇单正十二烷基酯	—	—	0.25
酸类 Acids	己酸	0.49	2.74	1.44
	辛酸	13.65	10.69	18.5
	壬酸	0.20	2.78	0.29
	癸酸	12.73	9.42	11.44
	9-癸烯酸	0.77	—	2.45
	月桂酸	0.75	2.27	2.93
	苯甲酸	—	4.22	—
	丁酸	—	—	0.75
其它 Other	甲苯	0.68	—	—
	2-庚酮	0.93	—	—
	3-甲基-1-丁醇	3.74	—	—
	苯乙烯	2.18	—	3.36
	2-壬酮	0.21	—	—
	E,E-2,4-庚二烯醛	0.36	—	—
	1,3-二叔丁基苯	3.74	10.46	9.78
	2,4-二特丁基苯酚	0.55	4.10	1.20
	二甲基砜	0.32	2.06	1.62
	2-乙基-1-己醇	—	20.30	—
	对甲苯酚	—	1.50	—
	3-甲基苯酚	—	1.57	—
	侧柏酮	—	—	2.12