]*>","")" /> Construction of Phospholipase D RNAi Vector of Flat Peach Fruit
Xinjiang Agricultural Sciences

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Construction of Phospholipase D RNAi Vector of Flat Peach Fruit

SHI Yuan-min;HE Tian-ming;YUAN Hai-ying   

  • Received:2012-08-25 Revised:2012-08-25 Online:2012-08-25 Published:2012-08-25

蟠桃磷脂酶D基因RNAi表达载体构建

史元敏;何天明;袁海英   

  1. 新疆农业大学林学与园艺学院,乌鲁木齐,830052





摘要： [目的]构建蟠桃磷脂酶D基因的RNAi表达载体.[方法]以英格尔蟠桃为材料,将CTAB改良法提取的蟠桃总RNA反转录成cDNA作为模板,通过RT - PCR扩增约347 bp的保守片段,将目标片段插入到pSK - int中间表达载体中,获得pSK - PLD - RNAi中间表达载体,然后将其转入植物双元表达载体pCAMBI1301中,构建该基因的shRNAi表达载体pCAMBIA - PLD - RNAi.[结果]经限制性内切酶酶切和测序鉴定证明蟠桃磷脂酶D基因的RNAi表达载体pCAMBIA - PLD - RNAi已构建成功.[结论]蟠桃磷脂酶D基因的RNAi表达载体的构建为进一步通过RNAi技术延长蟠桃果实的贮藏寿命奠定了基础.